pycom14g11810

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003064
Physical Location & Seq
Reverse (-)
14884166 .. 14884988
823 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGGAAGGAAGCACTAATTGTTACTTGGCAATGATACTGGTTCAGCTGATATACGGCGGATCAAACGTCCTCATGAAACTATCCCTTCAGGAAGGACTCAATCAGATTGTCTTCGTGGTTTACAGGCATGTTCTTGCCATGGTTTTGGTAGGACCATTTGCTTATATGCTTGAAAGGAAGCAGAGGCCTTCGCTCTCATTTTCGATAGCTGCAAAGATTTTTGCTCTTGCATCACTCGGGACTACCATCCATCTTAATATGTTCTATGCTGGTTTAGCATACACTTCTGCAACAGTTGGATGTGCCTTGAGTAATGTTATCCCTGCTTTCACGTTTCTAGTGGCAGTTCTTCTCAGGATGGAGAAACTGAACATTAGAAGCACTCGAGGTCAAGCTAAGGTGGTCGGAACCCTTCTTTGCCTTGGCGGTTCTCTTATTTTCACCTTCTGGAAAGGAGGATACCTATATAAAGGTTTTCTTGAGAAGCCACTGATAGATGGTTTCAACGCTGAGTCTGTTGGCAAGATTCAGCATGTCAAAGAAAACTGGATCAAGGGTTCTGTGCTGATTCTGACGAGTTACACTGCATGGAGTGCATGGCTAATCCTCCAGGTGATTGTATACTCCATTCAAGATGGTGCTCTCCAAACAAATATAATGTAA

Protein Analysis

221

Amino Acids

24.23

Weight (kDa)

9.47

Isoelectric Point (pI)

27.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 7 - 147 6.4e-14 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 621
AciI CCGC 2 cut(s) 57, 426
AclWI GGATC 2 cut(s) 67, 557
AcuI CTGAAG 1 cut(s) 71
AgsI TTSAA 3 cut(s) 173, 505, 632
AjnI CCWGG 1 cut(s) 609
AloI GAACNNNNNNTCC 2 cut(s) 541, 573
AluBI AGCT 3 cut(s) 46, 209, 395
AluI AGCT 3 cut(s) 46, 209, 395
Alw21I GWGCWC 1 cut(s) 643
AlwI GGATC 2 cut(s) 67, 557
Ama87I CYCGRG 2 cut(s) 236, 384
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 209
AspS9I GGNCC 1 cut(s) 152
AsuHPI GGTGA 2 cut(s) 433, 625
AvaI CYCGRG 2 cut(s) 236, 384
AvaII GGWCC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 1 cut(s) 196
BccI CCATC 5 cut(s) 254, 258, 352, 491, 629
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 1 cut(s) 611
BciVI GTATCC 1 cut(s) 452
BfaI CTAG 1 cut(s) 338
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 211
Bme1390I CCNGG 1 cut(s) 611
Bme18I GGWCC 1 cut(s) 152
BmeT110I CYCGRG 2 cut(s) 236, 384
BmgT120I GGNCC 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 409
BmrFI CCNGG 1 cut(s) 611
BmsI GCATC 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 103
BpmI CTGGAG 1 cut(s) 593
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 2 cut(s) 328, 500
BsaJI CCNNGG 2 cut(s) 138, 421
Bse1I ACTGG 2 cut(s) 42, 551
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 611
BseDI CCNNGG 2 cut(s) 138, 421
BseGI GGATG 3 cut(s) 246, 305, 363
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 2 cut(s) 367, 501
BseNI ACTGG 2 cut(s) 42, 551
BseXI GCAGC 1 cut(s) 196
BshFI GGCC 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 643
BsiHKCI CYCGRG 2 cut(s) 236, 384
BslFI GGGAC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 253
BsnI GGCC 1 cut(s) 187
BsoBI CYCGRG 2 cut(s) 236, 384
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 2 cut(s) 59, 549
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 2 cut(s) 57, 426
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 2 cut(s) 366, 502
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 409
BspPI GGATC 2 cut(s) 67, 557
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 2 cut(s) 42, 551
BssECI CCNNGG 2 cut(s) 138, 421
BssMI GATC 2 cut(s) 59, 549
BssNAI GTATAC 1 cut(s) 622
BssT1I CCWWGG 2 cut(s) 138, 421
Bst1107I GTATAC 1 cut(s) 622
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 1 cut(s) 295
BstAPI GCANNNNNTGC 1 cut(s) 593
BstDEI CTNAG 3 cut(s) 353, 396, 510
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 3 cut(s) 246, 305, 363
BstKTI GATC 2 cut(s) 62, 552
BstMBI GATC 2 cut(s) 59, 549
BstMWI GCNNNNNNNGC 2 cut(s) 275, 593
BstNI CCWGG 1 cut(s) 611
BstNSI RCATGY 2 cut(s) 131, 536
BstSCI CCNGG 1 cut(s) 609
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 145
BstZ17I GTATAC 1 cut(s) 622
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 3 cut(s) 246, 305, 363
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 2 cut(s) 488, 582
CciI TCATGA 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 152
CviAII CATG 6 cut(s) 73, 128, 139, 533, 588, 597
CviJI RGCY 6 cut(s) 46, 187, 209, 395, 487, 601
CviKI_1 RGCY 6 cut(s) 46, 187, 209, 395, 487, 601
DdeI CTNAG 3 cut(s) 353, 396, 510
DpnI GATC 2 cut(s) 61, 551
DpnII GATC 2 cut(s) 59, 549
EciI GGCGGA 1 cut(s) 72
Eco130I CCWWGG 2 cut(s) 138, 421
Eco147I AGGCCT 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 71
Eco88I CYCGRG 2 cut(s) 236, 384
EcoRII CCWGG 1 cut(s) 609
EcoT14I CCWWGG 2 cut(s) 138, 421
ErhI CCWWGG 2 cut(s) 138, 421
FaeI CATG 6 cut(s) 76, 131, 142, 536, 591, 600
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 1 cut(s) 253
FatI CATG 6 cut(s) 72, 127, 138, 532, 587, 596
FblI GTMKAC 1 cut(s) 621
Fnu4HI GCNGC 1 cut(s) 210
FokI GGATG 3 cut(s) 233, 312, 370
Fsp4HI GCNGC 1 cut(s) 210
FspBI CTAG 1 cut(s) 338
GluI GCNGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 593
HaeIII GGCC 1 cut(s) 187
Hin1II CATG 6 cut(s) 76, 131, 142, 536, 591, 600
HinfI GANTC 4 cut(s) 96, 512, 526, 568
HphI GGTGA 2 cut(s) 433, 625
Hpy166II GTNNAC 2 cut(s) 121, 622
Hpy188I TCNGA 3 cut(s) 105, 407, 573
Hpy188III TCNNGA 7 cut(s) 73, 89, 238, 355, 448, 479, 632
Hpy8I GTNNAC 2 cut(s) 121, 622
HpyAV CCTTC 5 cut(s) 86, 95, 198, 422, 454
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 2 cut(s) 66, 332
HpyCH4V TGCA 5 cut(s) 212, 230, 290, 587, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 593
HpyF3I CTNAG 3 cut(s) 353, 396, 510
HpySE526I ACGT 2 cut(s) 66, 332
Hsp92II CATG 6 cut(s) 76, 131, 142, 536, 591, 600
Kzo9I GATC 2 cut(s) 59, 549
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 1 cut(s) 239
MaeI CTAG 1 cut(s) 338
MaeII ACGT 2 cut(s) 66, 332
MaeIII GTNAC 2 cut(s) 20, 578
MalI GATC 2 cut(s) 61, 551
MboI GATC 2 cut(s) 59, 549
MboII GAAGA 2 cut(s) 103, 341
MhlI GDGCHC 1 cut(s) 643
MluCI AATT 1 cut(s) 16
MlyI GAGTC 2 cut(s) 90, 521
MmeI TCCRAC 2 cut(s) 277, 385
MnlI CCTC 5 cut(s) 80, 177, 380, 449, 617
MseI TTAA 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 611
MvaI CCWGG 1 cut(s) 611
MwoI GCNNNNNNNGC 2 cut(s) 275, 593
NcoI CCATGG 1 cut(s) 138
NdeII GATC 2 cut(s) 59, 549
NlaIII CATG 6 cut(s) 76, 131, 142, 536, 591, 600
NlaIV GGNNCC 1 cut(s) 409
NspI RCATGY 2 cut(s) 131, 536
PaeR7I CTCGAG 1 cut(s) 384
PagI TCATGA 1 cut(s) 72
PceI AGGCCT 1 cut(s) 187
PfeI GAWTC 2 cut(s) 526, 568
PkrI GCNGC 1 cut(s) 211
PleI GAGTC 2 cut(s) 90, 520
PpsI GAGTC 2 cut(s) 90, 520
Psp6I CCWGG 1 cut(s) 609
PspGI CCWGG 1 cut(s) 609
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 1 cut(s) 152
PspXI VCTCGAGB 1 cut(s) 384
PvuII CAGCTG 1 cut(s) 46
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 210
Sau3AI GATC 2 cut(s) 59, 549
Sau96I GGNCC 1 cut(s) 152
SchI GAGTC 2 cut(s) 90, 521
ScrFI CCNGG 1 cut(s) 611
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 1 cut(s) 239
Sfr274I CTCGAG 1 cut(s) 384
SinI GGWCC 1 cut(s) 152
SlaI CTCGAG 1 cut(s) 384
SmlI CTYRAG 3 cut(s) 307, 384, 479
SmoI CTYRAG 3 cut(s) 307, 384, 479
Sse9I AATT 1 cut(s) 16
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 2 cut(s) 57, 426
SspMI CTAG 1 cut(s) 338
StuI AGGCCT 1 cut(s) 187
StyD4I CCNGG 1 cut(s) 609
StyI CCWWGG 2 cut(s) 138, 421
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 2 cut(s) 69, 335
TaqI TCGA 2 cut(s) 203, 385
TasI AATT 1 cut(s) 16
TfiI GAWTC 2 cut(s) 526, 568
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 2 cut(s) 495, 589
TseI GCWGC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 89
TspRI CASTG 2 cut(s) 495, 589
VpaK11BI GGWCC 1 cut(s) 152
XceI RCATGY 2 cut(s) 131, 536
XhoI CTCGAG 1 cut(s) 384
XmiI GTMKAC 1 cut(s) 621
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.