pycom14g12350

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004579
Physical Location & Seq
Forward (+)
15370951 .. 15375285
4335 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2667 bp
ATGTTGTGTACTTTACGAGCACCTGCATTGAGAATGAGCTGTTACACAGGACTCCGGAGTGTTAATGCTTTAGATATTAGCGTAAAGCCCAGGCGAGACTTATTTTCTTCTGTGAAATTCGCAAACACTAAACGGTGTGAACGAGCTAGCAGATGTGTGCCTAAAGCCATGTTTGAGCGCTTCACAGAAAAAGCAATTAAAGTAATTATGCTTGCACAAGAGGAAGCAAGGCGACTAGGTCACAATTTTGTTGGCACGGAGCAGATACTTTTGGGTCTTATTGGTGAAGGGACTGGGATTGCTGCCAAGGTTCTCAAGTCCATGGGAATTAATCTTAAAGATGCACGAGTTGAAGTGGAGAAGATAATTGGAAGGGGTAGTGGATTTGTTGCTGTTGAAATTCCATTCACTCCTCGTGCAAAGCGAGTTTTGGAGCTGTCACTGGAGGAAGCTCGCCAACTTGGTCATAACTATATTGGATCCGAACATTTGCTTCTGGGTTTGCTTCGGGAGGGTGAGGGTGTAGCAGCTCGTGTTCTTGAGAATTTAGGTGCTGACCCTAGTAACATTCGCACACAGGTTATTCGTATGGTCGGTGAGAGCACAGAAGCTGTTGGTGCTGGTGTTGGAGGAGGGACCAGTGGTAATAAAACGCCGACTCTAGAAGAATATGGGACCAATTTGACTAAGCTAGCAGAGGAGGGTAAGTTGGATCCTGTTGTTGGAAGACAACAGCAAATTGAACGTGTCGTCCAAATTTTGGGTAGGAGGACTAAGAATAATCCCTGCCTTATTGGAGAACCTGGTGTTGGGAAGACTGCAATTGCAGAAGGTCTTGCTCAACGGATTTCAACTGGTGATGTTCCTGAAACAATAGAGGGAAAGAAGGTTATAACTCTGGATATGGGTCTTCTTGTTGCCGGTACAAAGTACCGTGGAGAGTTTGAGGAAAGATTGAAGAAGCTAATGGAGGAAATCAAACAAAGTGATGAGATTATTTTGTTTATTGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGGGCAATTGATGCTGCAAATATATTAAAACCGGCTCTTGCAAGAGGTGAACTGCAGTGCATTGGAGCCACAACTCTCGATGAGTACAGAAAGCACATTGAGAAAGATCCAGCTTTGGAAAGGCGATTCCAACCAGTCAAAGTTCCTGAACCAACTGTGGATGAAACCATTCAGATTTTGAGAGGGCTTAGGGAGCGTTACGAGATTCACCACAAGCTCCGCTACACTGATGAAGCCTTGGTATCTGCTGCACAGCTATCATACCAGTACATCAGTGATCGTTTTCTGCCTGATAAGGCAATTGACTTGGTTGATGAAGCTGGTTCTCGAGTTCGCCTTCGTCATGCACAGCTACCTGAAGAAGCCAGAGAGATTGAGAAAGAGCTGAGGGCGATCACTAAAGAGAAGAACGAGGCTGTTAGAAGTCAAGACTTTGAAAAGGCGGGGGAGTTACGTGACAAAGAAATGGACATAAAGGCACAGATCACAGCTGTTGTTGATAAAGGCAAGGAGATGAGCAAGGCAGAGAGTGAGGCAGGAGATGTAGGTCCTCTTGTGACTGAAGTGGATATTCAACATATTGTGTCCTCCTGGACTGGAATTCCAGTTGAGAAAGTATCAACCGATGAATCAGACCGCCTCCTCAAGATGGAAGAGACCCTCCATAAGCGAGTTATTGGTCAGGATGAAGCAGTCAAGGCTATTAGTCGTGCTATTCGCCGAGCCCGTGTTGGACTGAAGAATCCTAATCGTCCTATTGCCAGTTTCATCTTTTCTGGCCCCACTGGTGTTGGGAAATCTGAGCTCGCTAAAGCTTTGGCTGCTTACTACTTTGGTTCTGAAGAAGCCATGATTCGGCTTGATATGAGTGAGTTCATGGAAAGGCACACTGTTTCCAAGCTCATTGGTTCTCCCCCGGGTTATGTTGGTTACACTGAGGGAGGTCAGCTGACTGAAGCTGTTCGACGCCGTCCTTACACTGTAGTGCTTTTTGATGAAATTGAGAAGGCTCATCCCGATGTCTTCAACATGATGCTCCAAATACTCGAGGATGGAAGATTAACCGACAGCAAGGGCAGAACCGTGGATTTCAAGAATACACTTCTGATAATGACATCTAATGTTGGAAGCAGTGTGATCGAGAAAGGAGGCCGACGCATTGGATTTGACCTTGATTATGATGAGAAGGATAGCAGTTACAATAGAATTAAGAGCTTGGTGACGGAGGAACTAAAACAGTACTTCAGACCAGAGTTCTTGAATAGGTTGGACGAAATGATTGTTTTCCGACAACTCACCAAGCTGGAGGTGAAGGAGATAGCGGATATAATGCTCAAGGAGGTTTTCGAGAGGTTGAAGAAGAAAGAGATAGAGCTTCAAGTGACAGAGAGGTTCAGAGATAGAGTGGTTGACGAAGGATACAACCCGAGCTATGGAGCCAGGCCTCTAAGAAGAGCTATCATGAGACTCTTGGAGGACAGCATGGCTGAGAAGATGCTTGCAAGGGAGATCAAAGAAGGCGACTCGGTCATTGTGGATGTCGATTCTGATGGAAACGTGACCGTGCTCAACGGCAGCAGCGGTTCTCCCGAGTCCTTGCCAGAGCCGATTCCTGTTTGA

Protein Analysis

889

Amino Acids

98.86

Weight (kDa)

5.99

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 60 - 182 1.3e-36 Clp repeat (R) N-terminal domain
Clp_N PF02861 136 - 206 1.1e-16 Clp repeat (R) N-terminal domain
AAA PF00004 263 - 375 5.4e-14 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 402 - 503 2.5e-34 AAA lid domain
UVR PF02151 475 - 510 9.8e-08 UvrB/uvrC motif
AAA_2 PF07724 600 - 774 9.2e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 605 - 726 1.1e-11 AAA domain (dynein-related subfamily)
AAA PF00004 606 - 725 3.1e-07 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 781 - 861 1.1e-24 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 760
AccIII TCCGGA 1 cut(s) 54
AciI CCGC 5 cut(s) 1267, 1490, 1684, 2369, 2628
AclWI GGATC 5 cut(s) 474, 487, 707, 720, 1148
AcsI RAATTY 5 cut(s) 116, 399, 544, 756, 1647
AcuI CTGAAG 6 cut(s) 1425, 1629, 1805, 1908, 2022, 2275
AcyI GRCGYC 1 cut(s) 2014
AfaI GTAC 6 cut(s) 10, 925, 932, 1133, 1316, 2288
AfeI AGCGCT 1 cut(s) 179
AfiI CCNNNNNNNGG 6 cut(s) 722, 760, 809, 1696, 1902, 2480
AflIII ACRYGT 1 cut(s) 745
AjnI CCWGG 4 cut(s) 89, 802, 1637, 2486
AjuI GAANNNNNNNTTGG 2 cut(s) 792, 824
AleI CACNNNNGTG 1 cut(s) 2030
AloI GAACNNNNNNTCC 2 cut(s) 735, 767
Alw21I GWGCWC 5 cut(s) 22, 605, 1020, 1854, 2616
Alw26I GTCTC 3 cut(s) 90, 1697, 2506
Alw44I GTGCAC 1 cut(s) 1016
AlwI GGATC 5 cut(s) 474, 487, 707, 720, 1148
AlwNI CAGNNNCTG 1 cut(s) 611
Ama87I CYCGRG 5 cut(s) 1374, 1963, 2093, 2473, 2636
Aor13HI TCCGGA 1 cut(s) 54
Aor51HI AGCGCT 1 cut(s) 179
AoxI GGCC 3 cut(s) 1825, 2197, 2489
ApaLI GTGCAC 1 cut(s) 1016
ApeKI GCWGC 8 cut(s) 302, 527, 1040, 1061, 1295, 1868, 2622, 2625
ApoI RAATTY 5 cut(s) 116, 399, 544, 756, 1647
AseI ATTAAT 1 cut(s) 330
Asp700I GAANNNNTTC 2 cut(s) 1215, 2007
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 4 cut(s) 636, 675, 1595, 1826
AsuC2I CCSGG 2 cut(s) 1964, 1965
AsuHPI GGTGA 9 cut(s) 296, 527, 608, 869, 1106, 1247, 2278, 2335, 2368
AsuNHI GCTAGC 2 cut(s) 146, 691
AvaI CYCGRG 5 cut(s) 1374, 1963, 2093, 2473, 2636
AvaII GGWCC 3 cut(s) 636, 675, 1595
BaeGI GKGCMC 1 cut(s) 1020
BamHI GGATCC 2 cut(s) 479, 712
BanII GRGCYC 2 cut(s) 1774, 1854
BauI CACGAG 3 cut(s) 345, 414, 531
BbsI GAAGAC 4 cut(s) 733, 821, 902, 2062
Bbv12I GWGCWC 5 cut(s) 22, 605, 1020, 1854, 2616
BbvCI CCTCAGC 1 cut(s) 1433
BbvI GCAGC 8 cut(s) 289, 539, 1048, 1052, 1282, 1855, 2634, 2637
BccI CCATC 3 cut(s) 1690, 2093, 2591
BceAI ACGGC 2 cut(s) 2001, 2635
BcgI CGANNNNNNTGC 2 cut(s) 2167, 2201
BciT130I CCWGG 4 cut(s) 91, 804, 1639, 2488
BciVI GTATCC 1 cut(s) 2459
BcnI CCSGG 2 cut(s) 1964, 1965
BcoDI GTCTC 3 cut(s) 90, 1697, 2506
BfaI CTAG 5 cut(s) 147, 236, 561, 662, 692
BfmI CTRYAG 2 cut(s) 1100, 2028
BfoI RGCGCY 1 cut(s) 181
BfuAI ACCTGC 1 cut(s) 31
BfuI GTATCC 1 cut(s) 2459
BisI GCNGC 8 cut(s) 303, 528, 1041, 1062, 1296, 1869, 2623, 2626
BlsI GCNGC 8 cut(s) 304, 529, 1042, 1063, 1297, 1870, 2624, 2627
BmcAI AGTACT 1 cut(s) 2288
Bme1390I CCNGG 6 cut(s) 91, 804, 1639, 1964, 1965, 2488
Bme18I GGWCC 3 cut(s) 636, 675, 1595
BmeT110I CYCGRG 5 cut(s) 1374, 1963, 2093, 2473, 2636
BmgT120I GGNCC 4 cut(s) 636, 675, 1595, 1826
BmiI GGNNCC 7 cut(s) 481, 637, 676, 714, 1114, 1828, 2485
BmrFI CCNGG 6 cut(s) 91, 804, 1639, 1964, 1965, 2488
BmrI ACTGGG 1 cut(s) 303
BmsI GCATC 4 cut(s) 331, 1048, 2070, 2532
BmtI GCTAGC 2 cut(s) 150, 695
BmuI ACTGGG 1 cut(s) 303
BpiI GAAGAC 4 cut(s) 733, 821, 902, 2062
BpmI CTGGAG 2 cut(s) 464, 2372
Bpu10I CCTNAGC 2 cut(s) 1235, 1433
BpuEI CTTGAG 4 cut(s) 299, 560, 1676, 2366
BpuMI CCSGG 2 cut(s) 1964, 1965
BsaAI YACGTR 1 cut(s) 1502
BsaHI GRCGYC 1 cut(s) 2014
BsaI GGTCTC 1 cut(s) 1697
BsaJI CCNNGG 8 cut(s) 89, 306, 321, 934, 1284, 1962, 1963, 2130
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 692, 722
Bsc4I CCNNNNNNNGG 6 cut(s) 722, 760, 809, 1696, 1902, 2480
Bse118I RCCGGY 2 cut(s) 920, 1078
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 4 cut(s) 91, 804, 1639, 2488
BseDI CCNNGG 8 cut(s) 89, 306, 321, 934, 1284, 1962, 1963, 2130
BseGI GGATG 5 cut(s) 1213, 1738, 2059, 2104, 2590
BseLI CCNNNNNNNGG 6 cut(s) 722, 760, 809, 1696, 1902, 2480
BseMII CTCAG 4 cut(s) 1424, 1839, 1974, 2526
BseRI GAGGAG 4 cut(s) 402, 645, 713, 1679
BseSI GKGCMC 1 cut(s) 1020
BseXI GCAGC 8 cut(s) 289, 539, 1048, 1052, 1282, 1855, 2634, 2637
BsgI GTGCAG 1 cut(s) 1281
BshFI GGCC 3 cut(s) 1827, 2199, 2491
BsiHKAI GWGCWC 5 cut(s) 22, 605, 1020, 1854, 2616
BsiHKCI CYCGRG 5 cut(s) 1374, 1963, 2093, 2473, 2636
BsiSI CCGG 4 cut(s) 55, 921, 1079, 1964
BslFI GGGAC 3 cut(s) 304, 649, 688
BslI CCNNNNNNNGG 6 cut(s) 722, 760, 809, 1696, 1902, 2480
BsmAI GTCTC 3 cut(s) 90, 1697, 2506
BsmFI GGGAC 3 cut(s) 304, 649, 688
BsnI GGCC 3 cut(s) 1827, 2199, 2491
Bso31I GGTCTC 1 cut(s) 1697
BsoBI CYCGRG 5 cut(s) 1374, 1963, 2093, 2473, 2636
Bsp1286I GDGCHC 6 cut(s) 22, 605, 1020, 1774, 1854, 2616
Bsp13I TCCGGA 1 cut(s) 54
Bsp143I GATC 8 cut(s) 479, 712, 1153, 1324, 1440, 1530, 2184, 2556
Bsp19I CCATGG 1 cut(s) 321
BspACI CCGC 5 cut(s) 1267, 1490, 1684, 2369, 2628
BspANI GGCC 3 cut(s) 1827, 2199, 2491
BspCNI CTCAG 4 cut(s) 1425, 1840, 1975, 2527
BspEI TCCGGA 1 cut(s) 54
BspHI TCATGA 1 cut(s) 2508
BspLI GGNNCC 7 cut(s) 481, 637, 676, 714, 1114, 1828, 2485
BspMAI CTGCAG 1 cut(s) 1104
BspMI ACCTGC 1 cut(s) 31
BspOI GCTAGC 2 cut(s) 150, 695
BspPI GGATC 5 cut(s) 474, 487, 707, 720, 1148
BspQI GCTCTTC 1 cut(s) 2494
BspTNI GGTCTC 1 cut(s) 1697
BsrFI RCCGGY 2 cut(s) 920, 1078
BssAI RCCGGY 2 cut(s) 920, 1078
BssECI CCNNGG 8 cut(s) 89, 306, 321, 934, 1284, 1962, 1963, 2130
BssMI GATC 8 cut(s) 479, 712, 1153, 1324, 1440, 1530, 2184, 2556
BssNI GRCGYC 1 cut(s) 2014
BssSI CACGAG 3 cut(s) 345, 414, 531
BssT1I CCWWGG 3 cut(s) 306, 321, 1284
Bst2BI CACGAG 3 cut(s) 345, 414, 531
Bst2UI CCWGG 4 cut(s) 91, 804, 1639, 2488
Bst4CI ACNGT 8 cut(s) 135, 935, 1204, 1939, 2029, 2131, 2286, 2611
Bst6I CTCTTC 2 cut(s) 1695, 2494
BstACI GRCGYC 1 cut(s) 2014
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstBAI YACGTR 1 cut(s) 1502
BstC8I GCNNGC 6 cut(s) 148, 213, 454, 693, 1854, 2547
BstDEI CTNAG 8 cut(s) 687, 774, 1235, 1433, 1848, 1983, 2495, 2535
BstDSI CCRYGG 3 cut(s) 321, 934, 2130
BstF5I GGATG 5 cut(s) 1213, 1738, 2059, 2104, 2590
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 8 cut(s) 482, 715, 1156, 1327, 1443, 1533, 2187, 2559
BstMAI GTCTC 3 cut(s) 90, 1697, 2506
BstMBI GATC 8 cut(s) 479, 712, 1153, 1324, 1440, 1530, 2184, 2556
BstMWI GCNNNNNNNGC 7 cut(s) 617, 1040, 1049, 1058, 1240, 1745, 1868
BstNI CCWGG 4 cut(s) 91, 804, 1639, 2488
BstSCI CCNGG 6 cut(s) 89, 802, 1637, 1962, 1963, 2486
BstSFI CTRYAG 2 cut(s) 1100, 2028
BstSLI GKGCMC 1 cut(s) 1020
BstV1I GCAGC 8 cut(s) 289, 539, 1048, 1052, 1282, 1855, 2634, 2637
BstV2I GAAGAC 4 cut(s) 733, 821, 902, 2062
BstX2I RGATCY 3 cut(s) 479, 712, 1153
BstYI RGATCY 3 cut(s) 479, 712, 1153
BsuI GTATCC 1 cut(s) 2459
BsuRI GGCC 3 cut(s) 1827, 2199, 2491
BtgI CCRYGG 3 cut(s) 321, 934, 2130
BtsCI GGATG 5 cut(s) 1213, 1738, 2059, 2104, 2590
BtsI GCAGTG 2 cut(s) 1109, 2185
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 6 cut(s) 148, 213, 454, 693, 1854, 2547
CaiI CAGNNNCTG 1 cut(s) 611
CciI TCATGA 1 cut(s) 2508
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 2 cut(s) 920, 1078
Cfr13I GGNCC 4 cut(s) 636, 675, 1595, 1826
Cfr9I CCCGGG 1 cut(s) 1963
CseI GACGC 2 cut(s) 2022, 2211
CsiI ACCWGGT 1 cut(s) 802
Csp6I GTAC 6 cut(s) 9, 924, 931, 1132, 1315, 2287
CspCI CAANNNNNGTGG 2 cut(s) 1819, 1854
CviAII CATG 8 cut(s) 169, 322, 1391, 1897, 1924, 2077, 2509, 2530
CviQI GTAC 6 cut(s) 9, 924, 931, 1132, 1315, 2287
DdeI CTNAG 8 cut(s) 687, 774, 1235, 1433, 1848, 1983, 2495, 2535
DpnI GATC 8 cut(s) 481, 714, 1155, 1326, 1442, 1532, 2186, 2558
DpnII GATC 8 cut(s) 479, 712, 1153, 1324, 1440, 1530, 2184, 2556
Eam1104I CTCTTC 2 cut(s) 1695, 2494
EarI CTCTTC 2 cut(s) 1695, 2494
Ecl136II GAGCTC 1 cut(s) 1852
Eco130I CCWWGG 3 cut(s) 306, 321, 1284
Eco147I AGGCCT 1 cut(s) 2491
Eco24I GRGCYC 2 cut(s) 1774, 1854
Eco31I GGTCTC 1 cut(s) 1697
Eco47I GGWCC 3 cut(s) 636, 675, 1595
Eco47III AGCGCT 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 1852
Eco57I CTGAAG 6 cut(s) 1425, 1629, 1805, 1908, 2022, 2275
Eco88I CYCGRG 5 cut(s) 1374, 1963, 2093, 2473, 2636
EcoICRI GAGCTC 1 cut(s) 1852
EcoO109I RGGNCCY 1 cut(s) 1595
EcoRI GAATTC 1 cut(s) 1647
EcoRII CCWGG 4 cut(s) 89, 802, 1637, 2486
EcoT14I CCWWGG 3 cut(s) 306, 321, 1284
EcoT38I GRGCYC 2 cut(s) 1774, 1854
ErhI CCWWGG 3 cut(s) 306, 321, 1284
FaeI CATG 8 cut(s) 172, 325, 1394, 1900, 1927, 2080, 2512, 2533
FaqI GGGAC 3 cut(s) 304, 649, 688
FatI CATG 8 cut(s) 168, 321, 1390, 1896, 1923, 2076, 2508, 2529
FauI CCCGC 1 cut(s) 1483
Fnu4HI GCNGC 8 cut(s) 303, 528, 1041, 1062, 1296, 1869, 2623, 2626
FokI GGATG 5 cut(s) 1220, 1745, 2046, 2111, 2597
FriOI GRGCYC 2 cut(s) 1774, 1854
Fsp4HI GCNGC 8 cut(s) 303, 528, 1041, 1062, 1296, 1869, 2623, 2626
FspBI CTAG 5 cut(s) 147, 236, 561, 662, 692
GlaI GCGC 1 cut(s) 179
GluI GCNGC 8 cut(s) 303, 528, 1041, 1062, 1296, 1869, 2623, 2626
GsuI CTGGAG 2 cut(s) 464, 2372
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 3 cut(s) 1827, 2199, 2491
HapII CCGG 4 cut(s) 55, 921, 1079, 1964
HgaI GACGC 2 cut(s) 2022, 2211
HhaI GCGC 1 cut(s) 180
Hin1I GRCGYC 1 cut(s) 2014
Hin1II CATG 8 cut(s) 172, 325, 1394, 1900, 1927, 2080, 2512, 2533
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 2458
HindII GTYRAC 1 cut(s) 2458
HindIII AAGCTT 1 cut(s) 1860
HpaII CCGG 4 cut(s) 55, 921, 1079, 1964
HphI GGTGA 9 cut(s) 296, 527, 608, 869, 1106, 1247, 2278, 2335, 2368
Hpy166II GTNNAC 5 cut(s) 9, 140, 1018, 1097, 2458
Hpy8I GTNNAC 5 cut(s) 9, 140, 1018, 1097, 2458
Hpy99I CGWCG 2 cut(s) 2016, 2205
HpyCH4III ACNGT 8 cut(s) 135, 935, 1204, 1939, 2029, 2131, 2286, 2611
HpyCH4IV ACGT 3 cut(s) 745, 1501, 2604
HpyF10VI GCNNNNNNNGC 7 cut(s) 617, 1040, 1049, 1058, 1240, 1745, 1868
HpyF3I CTNAG 8 cut(s) 687, 774, 1235, 1433, 1848, 1983, 2495, 2535
HpySE526I ACGT 3 cut(s) 745, 1501, 2604
Hsp92I GRCGYC 1 cut(s) 2014
Hsp92II CATG 8 cut(s) 172, 325, 1394, 1900, 1927, 2080, 2512, 2533
HspAI GCGC 1 cut(s) 178
Kpn2I TCCGGA 1 cut(s) 54
Kzo9I GATC 8 cut(s) 479, 712, 1153, 1324, 1440, 1530, 2184, 2556
LguI GCTCTTC 1 cut(s) 2494
LmnI GCTCC 9 cut(s) 259, 433, 1031, 1037, 1112, 1240, 1269, 2088, 2483
Lsp1109I GCAGC 8 cut(s) 289, 539, 1048, 1052, 1282, 1855, 2634, 2637
LweI GCATC 4 cut(s) 331, 1048, 2070, 2532
MabI ACCWGGT 1 cut(s) 802
MaeI CTAG 5 cut(s) 147, 236, 561, 662, 692
MaeII ACGT 3 cut(s) 745, 1501, 2604
MalI GATC 8 cut(s) 481, 714, 1155, 1326, 1442, 1532, 2186, 2558
MboI GATC 8 cut(s) 479, 712, 1153, 1324, 1440, 1530, 2184, 2556
MfeI CAATTG 3 cut(s) 822, 1053, 1347
MflI RGATCY 3 cut(s) 479, 712, 1153
MhlI GDGCHC 6 cut(s) 22, 605, 1020, 1774, 1854, 2616
MlyI GAGTC 5 cut(s) 45, 652, 2508, 2564, 2648
MmeI TCCRAC 8 cut(s) 607, 690, 703, 1201, 1759, 2152, 2295, 2359
MroI TCCGGA 1 cut(s) 54
MroXI GAANNNNTTC 2 cut(s) 1215, 2007
MseI TTAA 8 cut(s) 63, 198, 330, 336, 1025, 1073, 2108, 2256
MslI CAYNNNNRTG 2 cut(s) 2030, 2064
MspA1I CMGCKG 3 cut(s) 1538, 1996, 2628
MspI CCGG 4 cut(s) 55, 921, 1079, 1964
MspR9I CCNGG 6 cut(s) 91, 804, 1639, 1964, 1965, 2488
MunI CAATTG 3 cut(s) 822, 1053, 1347
MvaI CCWGG 4 cut(s) 91, 804, 1639, 2488
MwoI GCNNNNNNNGC 7 cut(s) 617, 1040, 1049, 1058, 1240, 1745, 1868
NciI CCSGG 2 cut(s) 1964, 1965
NcoI CCATGG 1 cut(s) 321
NdeII GATC 8 cut(s) 479, 712, 1153, 1324, 1440, 1530, 2184, 2556
NheI GCTAGC 2 cut(s) 146, 691
NlaIII CATG 8 cut(s) 172, 325, 1394, 1900, 1927, 2080, 2512, 2533
NlaIV GGNNCC 7 cut(s) 481, 637, 676, 714, 1114, 1828, 2485
NmeAIII GCCGAG 1 cut(s) 1793
NmuCI GTSAC 7 cut(s) 239, 438, 1502, 1603, 2266, 2428, 2605
OliI CACNNNNGTG 1 cut(s) 2030
PaeR7I CTCGAG 2 cut(s) 1374, 2093
PagI TCATGA 1 cut(s) 2508
PaqCI CACCTGC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 2491
PciSI GCTCTTC 1 cut(s) 2494
PcsI WCGNNNNNNNCGW 3 cut(s) 139, 421, 1771
PdmI GAANNNNTTC 2 cut(s) 1215, 2007
PfeI GAWTC 7 cut(s) 1173, 1252, 1676, 1789, 1900, 2591, 2656
PflFI GACNNNGTC 3 cut(s) 237, 2016, 2573
PflMI CCANNNNNTGG 1 cut(s) 760
PfoI TCCNGGA 1 cut(s) 1637
PkrI GCNGC 8 cut(s) 304, 529, 1042, 1063, 1297, 1870, 2624, 2627
PleI GAGTC 5 cut(s) 45, 652, 2508, 2564, 2647
PpsI GAGTC 5 cut(s) 45, 652, 2508, 2564, 2647
Ppu21I YACGTR 1 cut(s) 1502
PpuMI RGGWCCY 1 cut(s) 1595
PshBI ATTAAT 1 cut(s) 330
PsiI TTATAA 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 1854
Psp5II RGGWCCY 1 cut(s) 1595
Psp6I CCWGG 4 cut(s) 89, 802, 1637, 2486
PspGI CCWGG 4 cut(s) 89, 802, 1637, 2486
PspN4I GGNNCC 7 cut(s) 481, 637, 676, 714, 1114, 1828, 2485
PspPI GGNCC 4 cut(s) 636, 675, 1595, 1826
PspPPI RGGWCCY 1 cut(s) 1595
PspXI VCTCGAGB 1 cut(s) 2093
PstI CTGCAG 1 cut(s) 1104
PstNI CAGNNNCTG 1 cut(s) 611
PsuI RGATCY 3 cut(s) 479, 712, 1153
PsyI GACNNNGTC 3 cut(s) 237, 2016, 2573
PvuII CAGCTG 2 cut(s) 1538, 1996
RsaI GTAC 6 cut(s) 10, 925, 932, 1133, 1316, 2288
RsaNI GTAC 6 cut(s) 9, 924, 931, 1132, 1315, 2287
RseI CAYNNNNRTG 2 cut(s) 2030, 2064
SacI GAGCTC 1 cut(s) 1854
SapI GCTCTTC 1 cut(s) 2494
SaqAI TTAA 8 cut(s) 63, 198, 330, 336, 1025, 1073, 2108, 2256
SatI GCNGC 8 cut(s) 303, 528, 1041, 1062, 1296, 1869, 2623, 2626
Sau3AI GATC 8 cut(s) 479, 712, 1153, 1324, 1440, 1530, 2184, 2556
Sau96I GGNCC 4 cut(s) 636, 675, 1595, 1826
ScaI AGTACT 1 cut(s) 2288
SchI GAGTC 5 cut(s) 45, 652, 2508, 2564, 2648
ScrFI CCNGG 6 cut(s) 91, 804, 1639, 1964, 1965, 2488
SduI GDGCHC 6 cut(s) 22, 605, 1020, 1774, 1854, 2616
SexAI ACCWGGT 1 cut(s) 802
SfaNI GCATC 4 cut(s) 331, 1048, 2070, 2532
SfcI CTRYAG 2 cut(s) 1100, 2028
Sfr274I CTCGAG 2 cut(s) 1374, 2093
SinI GGWCC 3 cut(s) 636, 675, 1595
SlaI CTCGAG 2 cut(s) 1374, 2093
SmaI CCCGGG 1 cut(s) 1965
SmiMI CAYNNNNRTG 2 cut(s) 2030, 2064
SmlI CTYRAG 6 cut(s) 314, 539, 1374, 1691, 2093, 2381
SmoI CTYRAG 6 cut(s) 314, 539, 1374, 1691, 2093, 2381
SseBI AGGCCT 1 cut(s) 2491
SsiI CCGC 5 cut(s) 1267, 1490, 1684, 2369, 2628
SspMI CTAG 5 cut(s) 147, 236, 561, 662, 692
SstI GAGCTC 1 cut(s) 1854
StuI AGGCCT 1 cut(s) 2491
StyD4I CCNGG 6 cut(s) 89, 802, 1637, 1962, 1963, 2486
StyI CCWWGG 3 cut(s) 306, 321, 1284
TaaI ACNGT 8 cut(s) 135, 935, 1204, 1939, 2029, 2131, 2286, 2611
TaiI ACGT 3 cut(s) 748, 1504, 2607
TaqI TCGA 7 cut(s) 1125, 1375, 2011, 2094, 2187, 2394, 2589
TaqII GACCGA 1 cut(s) 2563
TatI WGTACW 4 cut(s) 8, 1131, 1314, 2286
TfiI GAWTC 7 cut(s) 1173, 1252, 1676, 1789, 1900, 2591, 2656
Tru1I TTAA 8 cut(s) 63, 198, 330, 336, 1025, 1073, 2108, 2256
Tru9I TTAA 8 cut(s) 63, 198, 330, 336, 1025, 1073, 2108, 2256
TseFI GTSAC 7 cut(s) 239, 438, 1502, 1603, 2266, 2428, 2605
TseI GCWGC 8 cut(s) 302, 527, 1040, 1061, 1295, 1868, 2622, 2625
Tsp45I GTSAC 7 cut(s) 239, 438, 1502, 1603, 2266, 2428, 2605
TspDTI ATGAA 8 cut(s) 1224, 1293, 1377, 1689, 1749, 1804, 1912, 2058
TspGWI ACGGA 3 cut(s) 272, 859, 2285
TspMI CCCGGG 1 cut(s) 1963
Tth111I GACNNNGTC 3 cut(s) 237, 2016, 2573
Van91I CCANNNNNTGG 1 cut(s) 760
VneI GTGCAC 1 cut(s) 1016
VpaK11BI GGWCC 3 cut(s) 636, 675, 1595
VspI ATTAAT 1 cut(s) 330
XapI RAATTY 5 cut(s) 116, 399, 544, 756, 1647
XbaI TCTAGA 1 cut(s) 661
XhoI CTCGAG 2 cut(s) 1374, 2093
XmaI CCCGGG 1 cut(s) 1963
XmnI GAANNNNTTC 2 cut(s) 1215, 2007
XspI CTAG 5 cut(s) 147, 236, 561, 662, 692
ZrmI AGTACT 1 cut(s) 2288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.