pycom14g13650

Oxygen-independent coproporphyrinogen-III oxidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009349
Physical Location & Seq
Forward (+)
16513256 .. 16515283
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1530 bp
ATGAGTAACCATTATGCAGCTAAACCAGTAAAGCCATTGCCAATTTCGCAAAAACAGGCAGAAAGCTATTTGCACGCGCTCCTCGTTTTATTATCCCGTATAGGCGGCTCCATTATGCTTAAATCAAGCTTCGCTCCCATTTTCTCAACCTTTCCCGCCAAACGCCTATCGCCGCCGAAGCCACCGCCGGGCACTATCTTCACCTGCTTCGCACACACTGCGCCAACTGTTCGACAAAATGCCTCAACCAAAACCCCCGCCACCATTCTTCCGCTTCCCACTTCCGCCTACATCCACCTCCCTTTCTGCCGGAAGCGCTGCCACTACTGCGACTTCACAATCGTCGCCCTCGGCTCTGCTTCCACCCAAACGGACGACGACCCGCGAATGACAAACTACGTCGAGTTACTCTGCCGAGAAATCAACGCCACGAAAGTCGAAAATAAGACCAACACGCCTCTGGAGACGGTGTTTTTTGGCGGCGGCACGCCGTCTCTTGTGCCGCCAAAGCTCGTTAATTCCGTTTTAGAGACGTTGAGGTTGAAATTCGGGTTGAGTTCGGAGGCTGAGGTGTCGATGGAGATGGACCCGGGGACTTTCGATGCGAAGAAGATGAGGGGAATGATGGAGCTGGGTGTGAATAGGGTGTCATTGGGAGTTCAGGCGTTTCAGGAAGAGCTGTTGAAAGCTTGTGGGAGAGCTCATGGTCTTAGGGAAGTCTACGAGGCTATGGAGATTGTTGGGGAATGTGGGGTTGAGAACTGGAGCGTGGATCTTATCTCTTCGCTGCCTCACCAGACCCCGGAAATGTGGGAGGAGAGCTTGAGGCTGACGGTTGAAGCTCGTCCAACCCATGTTTCGGTTTATGATCTGCAAGTCGAACAAGGCACGAAATTCGGGTTGTTGTACACACCCGGGGAGTTCCCGTTGCCTTCGGAAACACGGTCAGCTGAGTTTTACAAGATGGCGTCTAGGATGCTTTCGGATGCGGGTTACAACCACTACGAAATTAGCAGTTACTGCAAGAGCGGATATCACTGCAGACACAATCTCACTTATTGGAAGAACAAACCTTTCTATGCATTTGGCCTTGGCTCTGCTAGCTATGTCGATGGTGTGAGGTTTTCGAGACCAAAGAGGATGAAAGAGTATACGGGTTATGTGGAGAATTTGGAAAATGGATTGGTGGATCGCTGTGAAAATAATCTTACTGCACAGGATATGGCCACGGATGTTGTTATGCTCTCTCTTAGAACTTCAAGAGGTCTGGATTTGAAGTCTTTCGGAGAAGCATATGGCAGCTCTCTCGTTCTTTCTCTTTGCAAGGCCTATAAACCCTATGTTGAAAGTGGGCATGTGGTTTTCTTGGATGAACAGAGAAGGGCCATCGCTGCACATGAATTCGACGCCTTGCAAGTAAACAAGGAGGAGACTGAAAGAAGTCCTGCCTACATTCGGCTTAGTGATCCAGACGGTTTCCTTTTGTCGAATGAATTGATATCGCTTGCATTTGCAGTTGTAGCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HemN_C Radical_SAM

Protein Analysis

510

Amino Acids

56.66

Weight (kDa)

6.52

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 97 - 277 2.5e-25 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 212
AccBSI CCGCTC 1 cut(s) 1029
AccI GTMKAC 2 cut(s) 720, 1151
AccII CGCG 2 cut(s) 77, 385
AclWI GGATC 3 cut(s) 780, 1197, 1460
AcoI YGGCCR 1 cut(s) 1224
AcsI RAATTY 4 cut(s) 545, 893, 1168, 1400
AcyI GRCGYC 2 cut(s) 968, 1407
AfaI GTAC 1 cut(s) 908
AfeI AGCGCT 1 cut(s) 317
AfiI CCNNNNNNNGG 4 cut(s) 188, 802, 859, 1455
AgsI TTSAA 6 cut(s) 544, 685, 839, 1260, 1276, 1346
AjuI GAANNNNNNNTTGG 2 cut(s) 841, 873
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 5 cut(s) 458, 498, 524, 1123, 1424
AlwI GGATC 3 cut(s) 780, 1197, 1460
AlwNI CAGNNNCTG 1 cut(s) 1020
Ama87I CYCGRG 2 cut(s) 589, 914
Aor51HI AGCGCT 1 cut(s) 317
AoxI GGCC 4 cut(s) 1087, 1224, 1326, 1383
ApeKI GCWGC 5 cut(s) 17, 318, 787, 1299, 1391
ApoI RAATTY 4 cut(s) 545, 893, 1168, 1400
Asp700I GAANNNNTTC 1 cut(s) 1280
AspLEI GCGC 3 cut(s) 79, 223, 318
AspS9I GGNCC 2 cut(s) 586, 1383
AsuC2I CCSGG 6 cut(s) 189, 590, 591, 803, 915, 916
AsuHPI GGTGA 2 cut(s) 193, 785
AsuNHI GCTAGC 1 cut(s) 1100
AvaI CYCGRG 2 cut(s) 589, 914
AvaII GGWCC 1 cut(s) 586
BaeGI GKGCMC 1 cut(s) 194
BalI TGGCCA 1 cut(s) 1226
BanII GRGCYC 1 cut(s) 703
Bbv12I GWGCWC 1 cut(s) 703
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 5 cut(s) 29, 305, 774, 1311, 1378
BccI CCATC 6 cut(s) 571, 577, 619, 958, 1106, 1394
BceAI ACGGC 1 cut(s) 475
BcgI CGANNNNNNTGC 4 cut(s) 877, 911, 1288, 1322
BcnI CCSGG 6 cut(s) 189, 590, 591, 803, 915, 916
BcoDI GTCTC 5 cut(s) 458, 498, 524, 1123, 1424
BfaI CTAG 2 cut(s) 972, 1101
BfmI CTRYAG 1 cut(s) 1039
BfoI RGCGCY 1 cut(s) 319
BfuAI ACCTGC 1 cut(s) 212
Bme1390I CCNGG 6 cut(s) 189, 590, 591, 803, 915, 916
Bme18I GGWCC 1 cut(s) 586
BmeT110I CYCGRG 2 cut(s) 589, 914
BmgT120I GGNCC 2 cut(s) 586, 1383
BmiI GGNNCC 2 cut(s) 109, 588
BmrFI CCNGG 6 cut(s) 189, 590, 591, 803, 915, 916
BmsI GCATC 3 cut(s) 592, 966, 976
BmtI GCTAGC 1 cut(s) 1104
BpmI CTGGAG 2 cut(s) 482, 784
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 844
BpuMI CCSGG 6 cut(s) 189, 590, 591, 803, 915, 916
BsaHI GRCGYC 2 cut(s) 968, 1407
BsaI GGTCTC 1 cut(s) 1123
BsaJI CCNNGG 8 cut(s) 349, 589, 590, 801, 914, 915, 1090, 1227
BsaXI ACNNNNNCTCC 4 cut(s) 455, 485, 911, 941
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 802, 859, 1455
Bse1I ACTGG 2 cut(s) 26, 767
Bse3DI GCAATG 1 cut(s) 35
BseDI CCNNGG 8 cut(s) 349, 589, 590, 801, 914, 915, 1090, 1227
BseGI GGATG 6 cut(s) 291, 981, 991, 1146, 1237, 1375
BseLI CCNNNNNNNGG 4 cut(s) 188, 802, 859, 1455
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 2 cut(s) 558, 942
BseNI ACTGG 2 cut(s) 26, 767
BseRI GAGGAG 3 cut(s) 71, 830, 1442
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 5 cut(s) 29, 305, 774, 1311, 1378
BseYI CCCAGC 1 cut(s) 631
BsgI GTGCAG 2 cut(s) 1197, 1377
Bsh1236I CGCG 2 cut(s) 77, 385
BshFI GGCC 4 cut(s) 1089, 1226, 1328, 1385
BsiHKAI GWGCWC 1 cut(s) 703
BsiHKCI CYCGRG 2 cut(s) 589, 914
BsiSI CCGG 5 cut(s) 188, 310, 590, 803, 915
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 4 cut(s) 188, 802, 859, 1455
BsmAI GTCTC 5 cut(s) 458, 498, 524, 1123, 1424
BsmBI CGTCTC 3 cut(s) 458, 498, 524
BsmFI GGGAC 1 cut(s) 607
BsnI GGCC 4 cut(s) 1089, 1226, 1328, 1385
Bso31I GGTCTC 1 cut(s) 1123
BsoBI CYCGRG 2 cut(s) 589, 914
Bsp1286I GDGCHC 2 cut(s) 194, 703
Bsp1407I TGTACA 1 cut(s) 906
Bsp143I GATC 4 cut(s) 772, 868, 1189, 1465
BspANI GGCC 4 cut(s) 1089, 1226, 1328, 1385
BspCNI CTCAG 2 cut(s) 559, 943
BspFNI CGCG 2 cut(s) 77, 385
BspLI GGNNCC 2 cut(s) 109, 588
BspMAI CTGCAG 1 cut(s) 1043
BspMI ACCTGC 1 cut(s) 212
BspOI GCTAGC 1 cut(s) 1104
BspPI GGATC 3 cut(s) 780, 1197, 1460
BspQI GCTCTTC 1 cut(s) 669
BspTNI GGTCTC 1 cut(s) 1123
BsrBI CCGCTC 1 cut(s) 1029
BsrDI GCAATG 1 cut(s) 35
BsrGI TGTACA 1 cut(s) 906
BsrI ACTGG 2 cut(s) 26, 767
BssECI CCNNGG 8 cut(s) 349, 589, 590, 801, 914, 915, 1090, 1227
BssMI GATC 4 cut(s) 772, 868, 1189, 1465
BssNAI GTATAC 1 cut(s) 1152
BssNI GRCGYC 2 cut(s) 968, 1407
BssT1I CCWWGG 1 cut(s) 1090
Bst1107I GTATAC 1 cut(s) 1152
Bst4CI ACNGT 5 cut(s) 229, 469, 835, 945, 1475
Bst6I CTCTTC 2 cut(s) 669, 787
BstACI GRCGYC 2 cut(s) 968, 1407
BstAPI GCANNNNNTGC 2 cut(s) 218, 1020
BstAUI TGTACA 1 cut(s) 906
BstC8I GCNNGC 4 cut(s) 75, 488, 1102, 1506
BstDEI CTNAG 5 cut(s) 567, 710, 951, 1250, 1460
BstDSI CCRYGG 1 cut(s) 1227
BstF5I GGATG 6 cut(s) 291, 981, 991, 1146, 1237, 1375
BstFNI CGCG 2 cut(s) 77, 385
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 3 cut(s) 79, 223, 318
BstKTI GATC 4 cut(s) 775, 871, 1192, 1468
BstMAI GTCTC 5 cut(s) 458, 498, 524, 1123, 1424
BstMBI GATC 4 cut(s) 772, 868, 1189, 1465
BstNSI RCATGY 1 cut(s) 1358
BstSCI CCNGG 6 cut(s) 187, 588, 589, 801, 913, 914
BstSFI CTRYAG 1 cut(s) 1039
BstSLI GKGCMC 1 cut(s) 194
BstUI CGCG 2 cut(s) 77, 385
BstV1I GCAGC 5 cut(s) 29, 305, 774, 1311, 1378
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
BstZ17I GTATAC 1 cut(s) 1152
BsuRI GGCC 4 cut(s) 1089, 1226, 1328, 1385
BtgI CCRYGG 1 cut(s) 1227
BtgZI GCGATG 1 cut(s) 1372
BtsCI GGATG 6 cut(s) 291, 981, 991, 1146, 1237, 1375
BtsI GCAGTG 2 cut(s) 216, 1036
BtsIMutI CAGTG 2 cut(s) 216, 1036
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 4 cut(s) 75, 488, 1102, 1506
CaiI CAGNNNCTG 1 cut(s) 1020
CfoI GCGC 3 cut(s) 79, 223, 318
Cfr13I GGNCC 2 cut(s) 586, 1383
Cfr9I CCCGGG 2 cut(s) 589, 914
CseI GACGC 2 cut(s) 957, 1415
Csp6I GTAC 1 cut(s) 907
CspCI CAANNNNNGTGG 2 cut(s) 1216, 1251
CviAII CATG 4 cut(s) 704, 854, 1355, 1397
CviQI GTAC 1 cut(s) 907
DdeI CTNAG 5 cut(s) 567, 710, 951, 1250, 1460
DpnI GATC 4 cut(s) 774, 870, 1191, 1467
DpnII GATC 4 cut(s) 772, 868, 1189, 1465
EaeI YGGCCR 1 cut(s) 1224
Eam1104I CTCTTC 2 cut(s) 669, 787
EarI CTCTTC 2 cut(s) 669, 787
EciI GGCGGA 1 cut(s) 274
Ecl136II GAGCTC 1 cut(s) 701
Eco130I CCWWGG 1 cut(s) 1090
Eco147I AGGCCT 1 cut(s) 1328
Eco24I GRGCYC 1 cut(s) 703
Eco31I GGTCTC 1 cut(s) 1123
Eco32I GATATC 2 cut(s) 1034, 1500
Eco47I GGWCC 1 cut(s) 586
Eco47III AGCGCT 1 cut(s) 317
Eco53kI GAGCTC 1 cut(s) 701
Eco88I CYCGRG 2 cut(s) 589, 914
EcoICRI GAGCTC 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 1400
EcoRV GATATC 2 cut(s) 1034, 1500
EcoT14I CCWWGG 1 cut(s) 1090
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 1090
Esp3I CGTCTC 3 cut(s) 458, 498, 524
FaeI CATG 4 cut(s) 707, 857, 1358, 1400
FaqI GGGAC 1 cut(s) 607
FatI CATG 4 cut(s) 703, 853, 1354, 1396
FauI CCCGC 4 cut(s) 163, 265, 390, 982
FauNDI CATATG 1 cut(s) 1294
FblI GTMKAC 2 cut(s) 720, 1151
FokI GGATG 6 cut(s) 278, 988, 998, 1153, 1244, 1382
FriOI GRGCYC 1 cut(s) 703
FspBI CTAG 2 cut(s) 972, 1101
GlaI GCGC 3 cut(s) 78, 222, 317
GsaI CCCAGC 1 cut(s) 635
GsuI CTGGAG 2 cut(s) 482, 784
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 4 cut(s) 1089, 1226, 1328, 1385
HapII CCGG 5 cut(s) 188, 310, 590, 803, 915
HgaI GACGC 2 cut(s) 957, 1415
HhaI GCGC 3 cut(s) 79, 223, 318
Hin1I GRCGYC 2 cut(s) 968, 1407
Hin1II CATG 4 cut(s) 707, 857, 1358, 1400
Hin6I GCGC 3 cut(s) 77, 221, 316
HinP1I GCGC 3 cut(s) 77, 221, 316
HindIII AAGCTT 2 cut(s) 127, 687
HpaII CCGG 5 cut(s) 188, 310, 590, 803, 915
HphI GGTGA 2 cut(s) 193, 785
Hpy166II GTNNAC 4 cut(s) 721, 909, 1152, 1420
Hpy188I TCNGA 4 cut(s) 562, 937, 985, 1286
Hpy188III TCNNGA 6 cut(s) 461, 671, 1128, 1260, 1268, 1469
Hpy8I GTNNAC 4 cut(s) 721, 909, 1152, 1420
Hpy99I CGWCG 4 cut(s) 347, 380, 404, 1409
HpyAV CCTTC 2 cut(s) 942, 1374
HpyCH4III ACNGT 5 cut(s) 229, 469, 835, 945, 1475
HpyCH4IV ACGT 2 cut(s) 399, 533
HpyF3I CTNAG 5 cut(s) 567, 710, 951, 1250, 1460
HpySE526I ACGT 2 cut(s) 399, 533
Hsp92I GRCGYC 2 cut(s) 968, 1407
Hsp92II CATG 4 cut(s) 707, 857, 1358, 1400
HspAI GCGC 3 cut(s) 77, 221, 316
Kzo9I GATC 4 cut(s) 772, 868, 1189, 1465
LguI GCTCTTC 1 cut(s) 669
LmnI GCTCC 6 cut(s) 84, 113, 139, 628, 765, 1528
Lsp1109I GCAGC 5 cut(s) 29, 305, 774, 1311, 1378
LweI GCATC 3 cut(s) 592, 966, 976
MaeI CTAG 2 cut(s) 972, 1101
MaeII ACGT 2 cut(s) 399, 533
MaeIII GTNAC 4 cut(s) 5, 405, 992, 1016
MalI GATC 4 cut(s) 774, 870, 1191, 1467
MbiI CCGCTC 1 cut(s) 1029
MboI GATC 4 cut(s) 772, 868, 1189, 1465
MboII GAAGA 7 cut(s) 190, 260, 619, 622, 686, 774, 1075
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 2 cut(s) 194, 703
MlsI TGGCCA 1 cut(s) 1226
MluCI AATT 8 cut(s) 42, 517, 545, 893, 1008, 1168, 1400, 1493
MluNI TGGCCA 1 cut(s) 1226
MmeI TCCRAC 1 cut(s) 872
Mox20I TGGCCA 1 cut(s) 1226
Mph1103I ATGCAT 1 cut(s) 1084
MroXI GAANNNNTTC 1 cut(s) 1280
MscI TGGCCA 1 cut(s) 1226
MseI TTAA 2 cut(s) 120, 516
Msp20I TGGCCA 1 cut(s) 1226
MspA1I CMGCKG 1 cut(s) 950
MspI CCGG 5 cut(s) 188, 310, 590, 803, 915
MspR9I CCNGG 6 cut(s) 189, 590, 591, 803, 915, 916
MvnI CGCG 2 cut(s) 77, 385
NciI CCSGG 6 cut(s) 189, 590, 591, 803, 915, 916
NdeI CATATG 1 cut(s) 1294
NdeII GATC 4 cut(s) 772, 868, 1189, 1465
NheI GCTAGC 1 cut(s) 1100
NlaIII CATG 4 cut(s) 707, 857, 1358, 1400
NlaIV GGNNCC 2 cut(s) 109, 588
NmeAIII GCCGAG 2 cut(s) 330, 440
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 1 cut(s) 1358
PaqCI CACCTGC 1 cut(s) 212
PceI AGGCCT 1 cut(s) 1328
PciSI GCTCTTC 1 cut(s) 669
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 519
PdmI GAANNNNTTC 1 cut(s) 1280
Psp124BI GAGCTC 1 cut(s) 703
PspFI CCCAGC 1 cut(s) 631
PspN4I GGNNCC 2 cut(s) 109, 588
PspPI GGNCC 2 cut(s) 586, 1383
PstI CTGCAG 1 cut(s) 1043
PstNI CAGNNNCTG 1 cut(s) 1020
PsuI RGATCY 1 cut(s) 772
PvuII CAGCTG 1 cut(s) 950
RsaI GTAC 1 cut(s) 908
RsaNI GTAC 1 cut(s) 907
SacI GAGCTC 1 cut(s) 703
SapI GCTCTTC 1 cut(s) 669
SaqAI TTAA 2 cut(s) 120, 516
Sau3AI GATC 4 cut(s) 772, 868, 1189, 1465
Sau96I GGNCC 2 cut(s) 586, 1383
ScrFI CCNGG 6 cut(s) 189, 590, 591, 803, 915, 916
SduI GDGCHC 2 cut(s) 194, 703
SfaNI GCATC 3 cut(s) 592, 966, 976
SfcI CTRYAG 1 cut(s) 1039
SinI GGWCC 1 cut(s) 586
SmaI CCCGGG 2 cut(s) 591, 916
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
Sse9I AATT 8 cut(s) 42, 517, 545, 893, 1008, 1168, 1400, 1493
SseBI AGGCCT 1 cut(s) 1328
SspMI CTAG 2 cut(s) 972, 1101
SstI GAGCTC 1 cut(s) 703
StuI AGGCCT 1 cut(s) 1328
StyD4I CCNGG 6 cut(s) 187, 588, 589, 801, 913, 914
StyI CCWWGG 1 cut(s) 1090
TaaI ACNGT 5 cut(s) 229, 469, 835, 945, 1475
TaiI ACGT 2 cut(s) 402, 536
TasI AATT 8 cut(s) 42, 517, 545, 893, 1008, 1168, 1400, 1493
TatI WGTACW 1 cut(s) 906
TauI GCSGC 5 cut(s) 108, 175, 483, 486, 505
Tru1I TTAA 2 cut(s) 120, 516
Tru9I TTAA 2 cut(s) 120, 516
TscAI CASTG 2 cut(s) 223, 1043
TseI GCWGC 5 cut(s) 17, 318, 787, 1299, 1391
TspDTI ATGAA 4 cut(s) 1157, 1386, 1413, 1506
TspGWI ACGGA 3 cut(s) 386, 511, 1244
TspMI CCCGGG 2 cut(s) 589, 914
TspRI CASTG 2 cut(s) 223, 1043
VpaK11BI GGWCC 1 cut(s) 586
XapI RAATTY 4 cut(s) 545, 893, 1168, 1400
XceI RCATGY 1 cut(s) 1358
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmaI CCCGGG 2 cut(s) 589, 914
XmiI GTMKAC 2 cut(s) 720, 1151
XmnI GAANNNNTTC 1 cut(s) 1280
XspI CTAG 2 cut(s) 972, 1101
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.