pycom14g14220

High-affinity nitrate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004983
Physical Location & Seq
Reverse (-)
16995555 .. 16996892
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGGCATCTCATGGGATTCTGTTGTCTTCACTTCTTCTGCTCTCCTGCTTATCCCAAACTTCCTATGGGATTGTCCTCTTTTCTTCTCTCAAGGATAGTCTCTTTGTTACTGCTTCACACACAGAAAACCAGGTTATGAAAGCAGGGATAGACAAGCTCACCGTTTCATGGGGGTTAAACCAGAGCTACGAAGCCGGCACCGACTCAACCTTCAAGACCATCAAGGTCAAGTTATGCTACGCGCCGGTGAGCCAAGTCGACCGCGGGTGGAGGAAGACCGTCGACAACCTCGCCAAGGACAAGACATGCCTGTTCAAGATCGTCGAAAGGTCGTACACCAGCAAGTCCAACGACTCCGTCGAGTGGACCATCGAGCGCGACGTGCCGGGCGCCACGTACTTCGTACGCGCCTACGCTTTCAACGCGGCGGGTCAGGAGGTGGCGTATGGTCAGACTACGAACGCCAAAAAGACCGCCAACTTGTTTGTGGTGGAGGGAATCAGCGGCCGCCACGTGTCGCTTGACATTGCATCGGTTGTCTTCAGTGTTTTCTCGGTTTTGTCCCTGGCTGGTTTCTTTATTGCAGAGAAGAGAAGGACAAAGTCGTCATAA

Protein Analysis

204

Amino Acids

22.25

Weight (kDa)

9.22

Isoelectric Point (pI)

25.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAR2 PF16974 27 - 169 9.2e-62 High-affinity nitrate transporter accessory, Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 604
AccB1I GGYRCC 2 cut(s) 197, 389
AccI GTMKAC 2 cut(s) 258, 282
AccII CGCG 5 cut(s) 242, 264, 378, 408, 425
AciI CCGC 7 cut(s) 262, 264, 425, 428, 474, 504, 508
AcoI YGGCCR 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 526
AcvI CACGTG 1 cut(s) 514
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 3 cut(s) 335, 398, 405
AfiI CCNNNNNNNGG 3 cut(s) 168, 295, 363
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 3 cut(s) 214, 316, 421
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 2 cut(s) 129, 564
AluBI AGCT 2 cut(s) 157, 186
AluI AGCT 2 cut(s) 157, 186
Alw26I GTCTC 1 cut(s) 104
AoxI GGCC 1 cut(s) 505
AspLEI GCGC 4 cut(s) 244, 378, 392, 410
AspS9I GGNCC 1 cut(s) 366
AsuC2I CCSGG 1 cut(s) 387
AsuHPI GGTGA 2 cut(s) 151, 259
AvaII GGWCC 1 cut(s) 366
BanI GGYRCC 2 cut(s) 197, 389
BbrPI CACGTG 1 cut(s) 514
BbsI GAAGAC 3 cut(s) 18, 281, 532
BccI CCATC 2 cut(s) 227, 377
BciT130I CCWGG 2 cut(s) 131, 566
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 104
BfoI RGCGCY 1 cut(s) 393
BisI GCNGC 3 cut(s) 426, 505, 508
BlsI GCNGC 3 cut(s) 427, 506, 509
Bme1390I CCNGG 3 cut(s) 131, 387, 566
Bme18I GGWCC 1 cut(s) 366
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 2 cut(s) 199, 391
BmrFI CCNGG 3 cut(s) 131, 387, 566
BmsI GCATC 2 cut(s) 14, 539
BpiI GAAGAC 3 cut(s) 18, 281, 532
BpuEI CTTGAG 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 387
BsaAI YACGTR 2 cut(s) 396, 514
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 3 cut(s) 262, 294, 564
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 295, 363
Bse118I RCCGGY 2 cut(s) 194, 244
Bse3DI GCAATG 1 cut(s) 525
BseBI CCWGG 2 cut(s) 131, 566
BseDI CCNNGG 3 cut(s) 262, 294, 564
BseLI CCNNNNNNNGG 3 cut(s) 168, 295, 363
BseMI GCAATG 1 cut(s) 525
BseX3I CGGCCG 1 cut(s) 505
Bsh1236I CGCG 5 cut(s) 242, 264, 378, 408, 425
Bsh1285I CGRYCG 2 cut(s) 262, 508
BshFI GGCC 1 cut(s) 507
BshNI GGYRCC 2 cut(s) 197, 389
BsiEI CGRYCG 2 cut(s) 262, 508
BsiSI CCGG 3 cut(s) 195, 245, 386
BsiWI CGTACG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 3 cut(s) 168, 295, 363
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 1 cut(s) 507
Bsp143I GATC 1 cut(s) 318
BspACI CCGC 7 cut(s) 262, 264, 425, 428, 474, 504, 508
BspANI GGCC 1 cut(s) 507
BspFNI CGCG 5 cut(s) 242, 264, 378, 408, 425
BspLI GGNNCC 2 cut(s) 199, 391
BspT107I GGYRCC 2 cut(s) 197, 389
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 2 cut(s) 194, 244
BssAI RCCGGY 2 cut(s) 194, 244
BssECI CCNNGG 3 cut(s) 262, 294, 564
BssMI GATC 1 cut(s) 318
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 131, 566
Bst4CI ACNGT 2 cut(s) 163, 280
Bst6I CTCTTC 1 cut(s) 584
BstACI GRCGYC 1 cut(s) 390
BstBAI YACGTR 2 cut(s) 396, 514
BstC8I GCNNGC 1 cut(s) 196
BstDSI CCRYGG 1 cut(s) 262
BstENI CCTNNNNNAGG 1 cut(s) 293
BstFNI CGCG 5 cut(s) 242, 264, 378, 408, 425
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 4 cut(s) 244, 378, 392, 410
BstKTI GATC 1 cut(s) 321
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 318
BstMCI CGRYCG 2 cut(s) 262, 508
BstMWI GCNNNNNNNGC 2 cut(s) 382, 422
BstNI CCWGG 2 cut(s) 131, 566
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 3 cut(s) 129, 385, 564
BstUI CGCG 5 cut(s) 242, 264, 378, 408, 425
BstV2I GAAGAC 3 cut(s) 18, 281, 532
BstZI CGGCCG 1 cut(s) 505
BsuRI GGCC 1 cut(s) 507
BtgI CCRYGG 1 cut(s) 262
BtrI CACGTC 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 550
Cac8I GCNNGC 1 cut(s) 196
CciNI GCGGCCGC 1 cut(s) 505
CfoI GCGC 4 cut(s) 244, 378, 392, 410
Cfr10I RCCGGY 2 cut(s) 194, 244
Cfr13I GGNCC 1 cut(s) 366
Cfr42I CCGCGG 1 cut(s) 265
CsiI ACCWGGT 1 cut(s) 129
Csp6I GTAC 3 cut(s) 334, 397, 404
CviAII CATG 3 cut(s) 11, 168, 306
CviJI RGCY 6 cut(s) 157, 186, 194, 252, 507, 569
CviKI_1 RGCY 6 cut(s) 157, 186, 194, 252, 507, 569
CviQI GTAC 3 cut(s) 334, 397, 404
DinI GGCGCC 1 cut(s) 391
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
DrdI GACNNNNNNGTC 1 cut(s) 604
DseDI GACNNNNNNGTC 1 cut(s) 604
EaeI YGGCCR 1 cut(s) 505
EagI CGGCCG 1 cut(s) 505
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
EclXI CGGCCG 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 366
Eco52I CGGCCG 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 526
Eco72I CACGTG 1 cut(s) 514
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoRII CCWGG 2 cut(s) 129, 564
EcoT14I CCWWGG 1 cut(s) 294
EgeI GGCGCC 1 cut(s) 391
EheI GGCGCC 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 3 cut(s) 14, 171, 309
FaiI YATR 8 cut(s) 12, 66, 137, 169, 235, 307, 447, 610
FaqI GGGAC 1 cut(s) 547
FatI CATG 3 cut(s) 10, 167, 305
FauI CCCGC 2 cut(s) 257, 421
FblI GTMKAC 2 cut(s) 258, 282
Fnu4HI GCNGC 3 cut(s) 426, 505, 508
Fsp4HI GCNGC 3 cut(s) 426, 505, 508
GlaI GCGC 4 cut(s) 243, 377, 391, 409
GluI GCNGC 3 cut(s) 426, 505, 508
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 1 cut(s) 507
HapII CCGG 3 cut(s) 195, 245, 386
HhaI GCGC 4 cut(s) 244, 378, 392, 410
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 3 cut(s) 14, 171, 309
Hin6I GCGC 4 cut(s) 242, 376, 390, 408
HinP1I GCGC 4 cut(s) 242, 376, 390, 408
HincII GTYRAC 2 cut(s) 259, 283
HindII GTYRAC 2 cut(s) 259, 283
HinfI GANTC 4 cut(s) 16, 203, 353, 498
HpaII CCGG 3 cut(s) 195, 245, 386
HphI GGTGA 2 cut(s) 151, 259
Hpy166II GTNNAC 4 cut(s) 259, 283, 336, 366
Hpy188I TCNGA 1 cut(s) 453
Hpy188III TCNNGA 3 cut(s) 214, 316, 434
Hpy8I GTNNAC 4 cut(s) 259, 283, 336, 366
Hpy99I CGWCG 4 cut(s) 284, 326, 362, 383
HpyAV CCTTC 2 cut(s) 220, 588
HpyCH4III ACNGT 2 cut(s) 163, 280
HpyCH4IV ACGT 3 cut(s) 381, 395, 513
HpyCH4V TGCA 2 cut(s) 530, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 422
HpySE526I ACGT 3 cut(s) 381, 395, 513
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 3 cut(s) 14, 171, 309
HspAI GCGC 4 cut(s) 242, 376, 390, 408
KasI GGCGCC 1 cut(s) 389
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
KspI CCGCGG 1 cut(s) 265
Kzo9I GATC 1 cut(s) 318
LweI GCATC 2 cut(s) 14, 539
MabI ACCWGGT 1 cut(s) 129
MaeII ACGT 3 cut(s) 381, 395, 513
MaeIII GTNAC 1 cut(s) 106
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MboII GAAGA 6 cut(s) 18, 26, 75, 286, 532, 601
Mly113I GGCGCC 1 cut(s) 390
MlyI GAGTC 2 cut(s) 197, 347
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 5 cut(s) 86, 264, 299, 430, 487
MroNI GCCGGC 1 cut(s) 194
MseI TTAA 1 cut(s) 176
MspA1I CMGCKG 2 cut(s) 264, 504
MspI CCGG 3 cut(s) 195, 245, 386
MspR9I CCNGG 3 cut(s) 131, 387, 566
MvaI CCWGG 2 cut(s) 131, 566
MvnI CGCG 5 cut(s) 242, 264, 378, 408, 425
MwoI GCNNNNNNNGC 2 cut(s) 382, 422
NaeI GCCGGC 1 cut(s) 196
NarI GGCGCC 1 cut(s) 390
NciI CCSGG 1 cut(s) 387
NdeII GATC 1 cut(s) 318
NgoMIV GCCGGC 1 cut(s) 194
NlaIII CATG 3 cut(s) 14, 171, 309
NlaIV GGNNCC 2 cut(s) 199, 391
NotI GCGGCCGC 1 cut(s) 505
NspI RCATGY 1 cut(s) 309
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 378
PdiI GCCGGC 1 cut(s) 196
PfeI GAWTC 2 cut(s) 16, 498
Pfl23II CGTACG 1 cut(s) 403
PflFI GACNNNGTC 2 cut(s) 356, 601
PkrI GCNGC 3 cut(s) 427, 506, 509
PleI GAGTC 2 cut(s) 197, 347
PluTI GGCGCC 1 cut(s) 393
PmaCI CACGTG 1 cut(s) 514
PmlI CACGTG 1 cut(s) 514
PpsI GAGTC 2 cut(s) 197, 347
Ppu21I YACGTR 2 cut(s) 396, 514
Psp6I CCWGG 2 cut(s) 129, 564
PspCI CACGTG 1 cut(s) 514
PspGI CCWGG 2 cut(s) 129, 564
PspLI CGTACG 1 cut(s) 403
PspN4I GGNNCC 2 cut(s) 199, 391
PspPI GGNCC 1 cut(s) 366
PsyI GACNNNGTC 2 cut(s) 356, 601
RsaI GTAC 3 cut(s) 335, 398, 405
RsaNI GTAC 3 cut(s) 334, 397, 404
SacII CCGCGG 1 cut(s) 265
SalI GTCGAC 2 cut(s) 257, 281
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 3 cut(s) 426, 505, 508
Sau3AI GATC 1 cut(s) 318
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 2 cut(s) 197, 347
ScrFI CCNGG 3 cut(s) 131, 387, 566
SexAI ACCWGGT 1 cut(s) 129
SfaNI GCATC 2 cut(s) 14, 539
SfoI GGCGCC 1 cut(s) 391
Sfr303I CCGCGG 1 cut(s) 265
SgrAI CRCCGGYG 1 cut(s) 244
SgrBI CCGCGG 1 cut(s) 265
SinI GGWCC 1 cut(s) 366
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
SsiI CCGC 7 cut(s) 262, 264, 425, 428, 474, 504, 508
SspDI GGCGCC 1 cut(s) 389
StyD4I CCNGG 3 cut(s) 129, 385, 564
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 163, 280
TaiI ACGT 3 cut(s) 384, 398, 516
TaqI TCGA 5 cut(s) 258, 282, 324, 360, 372
TauI GCSGC 3 cut(s) 428, 507, 510
TfiI GAWTC 2 cut(s) 16, 498
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 1 cut(s) 550
TspDTI ATGAA 2 cut(s) 152, 156
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 1 cut(s) 550
Tth111I GACNNNGTC 2 cut(s) 356, 601
VpaK11BI GGWCC 1 cut(s) 366
XagI CCTNNNNNAGG 1 cut(s) 293
XceI RCATGY 1 cut(s) 309
XcmI CCANNNNNNNNNTGG 2 cut(s) 62, 484
XmiI GTMKAC 2 cut(s) 258, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.