pycom14g19760

Prostaglandin E synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003258
Physical Location & Seq
Reverse (-)
21009613 .. 21012410
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
ATGAGAAGGGCTTCCACTCTCGCCTCACTCCCCCTCCTCTCCCGATCCCTTTCCACCGCCCAGGGCGGCGGCGCCGTCACCTCAGCCCCCGCCACCTACGGTCTTCTCCAGGTTGCTCTGTACGGGACCACCTCCACCGGTTCTCAGGCTCCTCGTCGATGGCTCGATTCGTTTTCCGGAGGTTCGGGTGGGAAGCTGGCAATCGGCGTCGCCGGAACTCTGGCTTCGGTCGCGGTCGCCACGTCGCTCGCTCCGGAGGCCTATGCCAAAGAGCCGCCTCCGGCTGCGCTTGTTCCCAAGGAAGTTGTGCTTTACCAGTACGAAGCCTGCCCTTTCTGCAACAAAGTTAAAGCATTCTTGGATTACCACGATATACCCTACAAAGTTGTGGAGGTCAACCCACTTAGTAAGAAAGAGATTAAATGGTCCGATTATCAGAAGGTCCCTATACTGATGGTGGATGGGGAGCAGCTGGTTGATTCATCAGCTATAATTGATCAGTTGAATAGCAAAATTGTTCCTGAGAGAGCAGCTGCTTCTTCCTCAGATGACGATGAAGAGAAAAAGTGGCGCCAGTGGGTTGATAATCACTTGGTGCATATGTTGTCACCAAACATATACCGAAATACGTCGGAGGCCCTTGAGTCCTTTGACTATATCACAAGCAATGGTAATTTTAGCTACACAGAAAAAATTTCAGTGAAGTATGCTGGTGCTGCAGCTATGTACTTTGTGTCAAAGAAACTAAAGAAGAAATATAATATTACTGATGAGCGTGCATCCCTGTATGAAGCAGCAGAAACATGGGTTGATGCTCTTAACGGGCGGGACTTCCTTGGGGGCTCCAAACCTAATTTGGCAGACCTTGCTGTTTTTGGGGTGCTAAGACCAATCCGTTATTTGAGGTCGGGTAAAGAAATGGTGGAGCATACACGCATCGGTGAATGGTACTCAAGAATGGAGCGTGCCGTGGGAGAGCCTGCAAGGATTAAGGAGTAA

Protein Analysis

333

Amino Acids

36.49

Weight (kDa)

8.28

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 102 - 156 2.2e-11 Glutaredoxin
GST_N_3 PF13417 104 - 170 2.5e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 109 - 169 1.9e-08 Glutathione S-transferase, N-terminal domain
GST_N_4 PF17172 111 - 202 1.6e-10 Glutathione S-transferase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 71, 570
AccII CGCG 1 cut(s) 233
AccIII TCCGGA 2 cut(s) 176, 253
AciI CCGC 7 cut(s) 57, 66, 69, 90, 233, 275, 826
AclWI GGATC 1 cut(s) 39
AcsI RAATTY 1 cut(s) 693
AcyI GRCGYC 3 cut(s) 72, 207, 571
AdeI CACNNNGTG 1 cut(s) 595
AfaI GTAC 4 cut(s) 122, 320, 728, 950
AgeI ACCGGT 1 cut(s) 137
AgsI TTSAA 1 cut(s) 505
AjiI CACGTC 1 cut(s) 243
AjnI CCWGG 2 cut(s) 60, 108
AluBI AGCT 6 cut(s) 196, 472, 488, 533, 681, 722
AluI AGCT 6 cut(s) 196, 472, 488, 533, 681, 722
AlwI GGATC 1 cut(s) 39
Aor13HI TCCGGA 2 cut(s) 176, 253
AoxI GGCC 2 cut(s) 258, 636
ApeKI GCWGC 7 cut(s) 284, 469, 530, 533, 716, 719, 794
ApoI RAATTY 1 cut(s) 693
AsiGI ACCGGT 1 cut(s) 137
Asp700I GAANNNNTTC 1 cut(s) 10
AspLEI GCGC 3 cut(s) 74, 289, 573
AspS9I GGNCC 4 cut(s) 126, 426, 442, 637
AsuHPI GGTGA 3 cut(s) 70, 600, 953
AvaII GGWCC 3 cut(s) 126, 426, 442
BanI GGYRCC 2 cut(s) 71, 570
BanII GRGCYC 1 cut(s) 845
BbsI GAAGAC 1 cut(s) 95
BbvCI CCTCAGC 1 cut(s) 82
BbvI GCAGC 7 cut(s) 271, 481, 520, 542, 703, 731, 806
BccI CCATC 3 cut(s) 153, 448, 455
BceAI ACGGC 2 cut(s) 59, 953
BciT130I CCWGG 2 cut(s) 62, 110
BclI TGATCA 1 cut(s) 496
BfmI CTRYAG 1 cut(s) 717
BfoI RGCGCY 2 cut(s) 75, 574
Bme1390I CCNGG 2 cut(s) 62, 110
Bme18I GGWCC 3 cut(s) 126, 426, 442
BmgBI CACGTC 1 cut(s) 243
BmgT120I GGNCC 4 cut(s) 126, 426, 442, 637
BmiI GGNNCC 6 cut(s) 73, 127, 150, 444, 572, 844
BmrFI CCNGG 2 cut(s) 62, 110
BmsI GCATC 3 cut(s) 788, 802, 945
BpiI GAAGAC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 92
Bpu10I CCTNAGC 1 cut(s) 82
BpuEI CTTGAG 2 cut(s) 662, 937
BsaHI GRCGYC 3 cut(s) 72, 207, 571
BsaJI CCNNGG 5 cut(s) 60, 61, 297, 835, 969
BsaWI WCCGGW 3 cut(s) 137, 176, 253
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 316, 574
Bse3DI GCAATG 1 cut(s) 673
BseAI TCCGGA 2 cut(s) 176, 253
BseBI CCWGG 2 cut(s) 62, 110
BseDI CCNNGG 5 cut(s) 60, 61, 297, 835, 969
BseGI GGATG 2 cut(s) 466, 779
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 4 cut(s) 96, 158, 513, 558
BseNI ACTGG 2 cut(s) 316, 574
BseRI GAGGAG 2 cut(s) 26, 141
BseXI GCAGC 7 cut(s) 271, 481, 520, 542, 703, 731, 806
Bsh1236I CGCG 1 cut(s) 233
Bsh1285I CGRYCG 2 cut(s) 231, 237
BshFI GGCC 2 cut(s) 260, 638
BshNI GGYRCC 2 cut(s) 71, 570
BshTI ACCGGT 1 cut(s) 137
BsiEI CGRYCG 2 cut(s) 231, 237
BsiSI CCGG 5 cut(s) 138, 177, 213, 254, 281
BslFI GGGAC 3 cut(s) 139, 428, 842
BsmFI GGGAC 3 cut(s) 139, 428, 842
BsmI GAATGC 1 cut(s) 353
BsnI GGCC 2 cut(s) 260, 638
Bsp1286I GDGCHC 1 cut(s) 845
Bsp13I TCCGGA 2 cut(s) 176, 253
Bsp143I GATC 2 cut(s) 44, 496
BspACI CCGC 7 cut(s) 57, 66, 69, 90, 233, 275, 826
BspANI GGCC 2 cut(s) 260, 638
BspCNI CTCAG 4 cut(s) 95, 157, 514, 557
BspEI TCCGGA 2 cut(s) 176, 253
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 6 cut(s) 73, 127, 150, 444, 572, 844
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 1 cut(s) 39
BspT107I GGYRCC 2 cut(s) 71, 570
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 2 cut(s) 316, 574
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 5 cut(s) 60, 61, 297, 835, 969
BssMI GATC 2 cut(s) 44, 496
BssNI GRCGYC 3 cut(s) 72, 207, 571
BssT1I CCWWGG 2 cut(s) 297, 835
Bst2UI CCWGG 2 cut(s) 62, 110
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 3 cut(s) 72, 207, 571
BstAPI GCANNNNNTGC 1 cut(s) 866
BstC8I GCNNGC 6 cut(s) 198, 249, 328, 777, 966, 981
BstDEI CTNAG 6 cut(s) 82, 144, 404, 522, 544, 884
BstDSI CCRYGG 1 cut(s) 969
BstF5I GGATG 2 cut(s) 466, 779
BstFNI CGCG 1 cut(s) 233
BstH2I RGCGCY 2 cut(s) 75, 574
BstHHI GCGC 3 cut(s) 74, 289, 573
BstKTI GATC 2 cut(s) 47, 499
BstMBI GATC 2 cut(s) 44, 496
BstMCI CGRYCG 2 cut(s) 231, 237
BstMWI GCNNNNNNNGC 5 cut(s) 230, 257, 336, 716, 866
BstNI CCWGG 2 cut(s) 62, 110
BstSCI CCNGG 2 cut(s) 60, 108
BstSFI CTRYAG 1 cut(s) 717
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 7 cut(s) 271, 481, 520, 542, 703, 731, 806
BstV2I GAAGAC 1 cut(s) 95
BsuRI GGCC 2 cut(s) 260, 638
BtgI CCRYGG 1 cut(s) 969
BtrI CACGTC 1 cut(s) 243
BtsCI GGATG 2 cut(s) 466, 779
BtsIMutI CAGTG 2 cut(s) 581, 705
Cac8I GCNNGC 6 cut(s) 198, 249, 328, 777, 966, 981
CfoI GCGC 3 cut(s) 74, 289, 573
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 4 cut(s) 126, 426, 442, 637
CseI GACGC 1 cut(s) 196
Csp6I GTAC 4 cut(s) 121, 319, 727, 949
CspAI ACCGGT 1 cut(s) 137
CviAII CATG 1 cut(s) 804
CviQI GTAC 4 cut(s) 121, 319, 727, 949
DdeI CTNAG 6 cut(s) 82, 144, 404, 522, 544, 884
DinI GGCGCC 2 cut(s) 73, 572
DpnI GATC 2 cut(s) 46, 498
DpnII GATC 2 cut(s) 44, 496
DraIII CACNNNGTG 1 cut(s) 595
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco130I CCWWGG 2 cut(s) 297, 835
Eco147I AGGCCT 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 845
Eco47I GGWCC 3 cut(s) 126, 426, 442
EcoO109I RGGNCCY 2 cut(s) 442, 637
EcoRII CCWGG 2 cut(s) 60, 108
EcoT14I CCWWGG 2 cut(s) 297, 835
EcoT38I GRGCYC 1 cut(s) 845
EgeI GGCGCC 2 cut(s) 73, 572
EheI GGCGCC 2 cut(s) 73, 572
ErhI CCWWGG 2 cut(s) 297, 835
FaeI CATG 1 cut(s) 807
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FaqI GGGAC 3 cut(s) 139, 428, 842
FatI CATG 1 cut(s) 803
FauI CCCGC 2 cut(s) 97, 819
FauNDI CATATG 1 cut(s) 600
FbaI TGATCA 1 cut(s) 496
FokI GGATG 2 cut(s) 473, 766
FriOI GRGCYC 1 cut(s) 845
GlaI GCGC 3 cut(s) 73, 288, 572
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 2 cut(s) 75, 574
HaeIII GGCC 2 cut(s) 260, 638
HapII CCGG 5 cut(s) 138, 177, 213, 254, 281
HgaI GACGC 1 cut(s) 196
HhaI GCGC 3 cut(s) 74, 289, 573
Hin1I GRCGYC 3 cut(s) 72, 207, 571
Hin1II CATG 1 cut(s) 807
Hin6I GCGC 3 cut(s) 72, 287, 571
HinP1I GCGC 3 cut(s) 72, 287, 571
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 3 cut(s) 167, 479, 644
HpaII CCGG 5 cut(s) 138, 177, 213, 254, 281
HphI GGTGA 3 cut(s) 70, 600, 953
Hpy166II GTNNAC 1 cut(s) 397
Hpy188I TCNGA 4 cut(s) 430, 438, 547, 634
Hpy188III TCNNGA 5 cut(s) 42, 177, 254, 521, 954
Hpy8I GTNNAC 1 cut(s) 397
Hpy99I CGWCG 4 cut(s) 159, 212, 247, 634
HpyAV CCTTC 1 cut(s) 433
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 2 cut(s) 242, 629
HpyCH4V TGCA 5 cut(s) 339, 598, 719, 779, 983
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 257, 336, 716, 866
HpyF3I CTNAG 6 cut(s) 82, 144, 404, 522, 544, 884
HpySE526I ACGT 2 cut(s) 242, 629
Hsp92I GRCGYC 3 cut(s) 72, 207, 571
Hsp92II CATG 1 cut(s) 807
HspAI GCGC 3 cut(s) 72, 287, 571
KasI GGCGCC 2 cut(s) 71, 570
Kpn2I TCCGGA 2 cut(s) 176, 253
Ksp22I TGATCA 1 cut(s) 496
Kzo9I GATC 2 cut(s) 44, 496
LmnI GCTCC 6 cut(s) 154, 256, 466, 848, 925, 961
Lsp1109I GCAGC 7 cut(s) 271, 481, 520, 542, 703, 731, 806
LweI GCATC 3 cut(s) 788, 802, 945
MaeII ACGT 2 cut(s) 242, 629
MaeIII GTNAC 2 cut(s) 76, 606
MalI GATC 2 cut(s) 46, 498
MboI GATC 2 cut(s) 44, 496
MboII GAAGA 4 cut(s) 95, 531, 569, 763
MhlI GDGCHC 1 cut(s) 845
MluCI AATT 5 cut(s) 492, 513, 673, 693, 853
Mly113I GGCGCC 2 cut(s) 72, 571
MlyI GAGTC 1 cut(s) 653
MmeI TCCRAC 1 cut(s) 612
MroI TCCGGA 2 cut(s) 176, 253
MroXI GAANNNNTTC 1 cut(s) 10
MseI TTAA 4 cut(s) 348, 420, 819, 990
MspA1I CMGCKG 2 cut(s) 472, 533
MspI CCGG 5 cut(s) 138, 177, 213, 254, 281
MspR9I CCNGG 2 cut(s) 62, 110
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 2 cut(s) 62, 110
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 5 cut(s) 230, 257, 336, 716, 866
NarI GGCGCC 2 cut(s) 72, 571
NdeI CATATG 1 cut(s) 600
NdeII GATC 2 cut(s) 44, 496
NlaIII CATG 1 cut(s) 807
NlaIV GGNNCC 6 cut(s) 73, 127, 150, 444, 572, 844
NmuCI GTSAC 2 cut(s) 76, 606
PasI CCCWGGG 1 cut(s) 61
PceI AGGCCT 1 cut(s) 260
PctI GAATGC 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 167, 479
PinAI ACCGGT 1 cut(s) 137
PleI GAGTC 1 cut(s) 652
PluTI GGCGCC 2 cut(s) 75, 574
PpsI GAGTC 1 cut(s) 652
PpuMI RGGWCCY 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 2 cut(s) 60, 108
PspGI CCWGG 2 cut(s) 60, 108
PspN4I GGNNCC 6 cut(s) 73, 127, 150, 444, 572, 844
PspPI GGNCC 4 cut(s) 126, 426, 442, 637
PspPPI RGGWCCY 1 cut(s) 442
PstI CTGCAG 1 cut(s) 721
PvuII CAGCTG 2 cut(s) 472, 533
RsaI GTAC 4 cut(s) 122, 320, 728, 950
RsaNI GTAC 4 cut(s) 121, 319, 727, 949
SaqAI TTAA 4 cut(s) 348, 420, 819, 990
Sau3AI GATC 2 cut(s) 44, 496
Sau96I GGNCC 4 cut(s) 126, 426, 442, 637
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 2 cut(s) 62, 110
SduI GDGCHC 1 cut(s) 845
SfaNI GCATC 3 cut(s) 788, 802, 945
SfcI CTRYAG 1 cut(s) 717
SfoI GGCGCC 2 cut(s) 73, 572
SinI GGWCC 3 cut(s) 126, 426, 442
SmlI CTYRAG 2 cut(s) 641, 952
SmoI CTYRAG 2 cut(s) 641, 952
Sse9I AATT 5 cut(s) 492, 513, 673, 693, 853
SseBI AGGCCT 1 cut(s) 260
SsiI CCGC 7 cut(s) 57, 66, 69, 90, 233, 275, 826
SspDI GGCGCC 2 cut(s) 71, 570
SspI AATATT 1 cut(s) 763
StuI AGGCCT 1 cut(s) 260
StyD4I CCNGG 2 cut(s) 60, 108
StyI CCWWGG 2 cut(s) 297, 835
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 2 cut(s) 245, 632
TaqI TCGA 2 cut(s) 157, 165
TaqII GACCGA 1 cut(s) 217
TasI AATT 5 cut(s) 492, 513, 673, 693, 853
TatI WGTACW 1 cut(s) 726
TauI GCSGC 3 cut(s) 69, 72, 277
TfiI GAWTC 2 cut(s) 167, 479
Tru1I TTAA 4 cut(s) 348, 420, 819, 990
Tru9I TTAA 4 cut(s) 348, 420, 819, 990
TscAI CASTG 2 cut(s) 581, 705
TseFI GTSAC 2 cut(s) 76, 606
TseI GCWGC 7 cut(s) 284, 469, 530, 533, 716, 719, 794
Tsp45I GTSAC 2 cut(s) 76, 606
TspDTI ATGAA 3 cut(s) 471, 570, 804
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 2 cut(s) 581, 705
VpaK11BI GGWCC 3 cut(s) 126, 426, 442
XapI RAATTY 1 cut(s) 693
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.