pycom15g01800

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002741
Physical Location & Seq
Reverse (-)
1039146 .. 1041282
2137 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGACGAGCTCCTCCGCCACTTCACGCAAGGCTTTGAGCAAGATCGCCTGCAATCGGCTGCAGAAAGAGCTGTTGGAGTGGCAGGTGAATCCTCCGGCAGGTTTCAAGCACAAAGTCACCGATAATCTTCAGAGATGGGTCATCGAAGTCACCGGAGCCCCGGGAACTCTATACGCTAACGAAATTTATCAGCTCCAAGTCGATTTCCCCGAGCATTATCCCATGGAGGCACCACAGGTGATTTTTGTCCCTCCGGCTCCACTGCATCCTCACATTTACAGCAATGGCCATATCTGTTTAGATATTCTATATGATTCATGGCCCCCAGCCATGACGGTTAGTTCCATCTGTATCAGCATTCTCTCCATGTTATCGAGTTCCACGGTGAAGCAACGCCCAGCGGATAACGATCGATACGTGAAGAACTGCAGGAACGGCAGATCTCCCAAGGAGACGAGATGGTGGTTCCATGATGACAAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

162

Amino Acids

18.36

Weight (kDa)

8.35

Isoelectric Point (pI)

65.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 19 - 154 2.6e-38 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 2 cut(s) 73, 89
AccB1I GGYRCC 1 cut(s) 229
AciI CCGC 2 cut(s) 15, 401
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 54, 98
AgsI TTSAA 1 cut(s) 106
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 9, 70, 193
AluI AGCT 3 cut(s) 9, 70, 193
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 446
Ama87I CYCGRG 2 cut(s) 160, 209
AoxI GGCC 2 cut(s) 286, 320
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 1 cut(s) 183
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 2 cut(s) 161, 162
AsuHPI GGTGA 5 cut(s) 97, 109, 142, 250, 397
AvaI CYCGRG 2 cut(s) 160, 209
BalI TGGCCA 1 cut(s) 288
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 2 cut(s) 11, 160
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 45
BccI CCATC 3 cut(s) 129, 353, 453
BceAI ACGGC 1 cut(s) 451
BcnI CCSGG 2 cut(s) 161, 162
BcoDI GTCTC 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 59, 427
BfuAI ACCTGC 2 cut(s) 73, 89
BglII AGATCT 1 cut(s) 440
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 2 cut(s) 161, 162
BmeT110I CYCGRG 2 cut(s) 160, 209
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 5 cut(s) 157, 231, 258, 323, 467
BmrFI CCNGG 2 cut(s) 161, 162
BmsI GCATC 1 cut(s) 274
BpuMI CCSGG 2 cut(s) 161, 162
Bsa29I ATCGAT 1 cut(s) 412
BsaAI YACGTR 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 408
BsaJI CCNNGG 5 cut(s) 159, 160, 222, 381, 447
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 98
Bse3DI GCAATG 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 5 cut(s) 159, 160, 222, 381, 447
BseGI GGATG 1 cut(s) 265
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 54, 98
BseMI GCAATG 1 cut(s) 289
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 2 cut(s) 325, 397
Bsh1285I CGRYCG 1 cut(s) 412
BshFI GGCC 2 cut(s) 288, 322
BshNI GGYRCC 1 cut(s) 229
BshVI ATCGAT 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 2 cut(s) 160, 209
BsiSI CCGG 4 cut(s) 95, 153, 161, 254
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 54, 98
BsmAI GTCTC 1 cut(s) 446
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 233
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 288, 322
BsoBI CYCGRG 2 cut(s) 160, 209
Bsp1286I GDGCHC 2 cut(s) 11, 160
Bsp143I GATC 3 cut(s) 42, 409, 440
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 2 cut(s) 15, 401
BspANI GGCC 2 cut(s) 288, 322
BspDI ATCGAT 1 cut(s) 412
BspLI GGNNCC 5 cut(s) 157, 231, 258, 323, 467
BspMAI CTGCAG 2 cut(s) 63, 431
BspMI ACCTGC 2 cut(s) 73, 89
BspT107I GGYRCC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 5 cut(s) 159, 160, 222, 381, 447
BssMI GATC 3 cut(s) 42, 409, 440
BssT1I CCWWGG 2 cut(s) 222, 447
Bst4CI ACNGT 2 cut(s) 337, 385
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 49
BstDSI CCRYGG 2 cut(s) 222, 381
BstENI CCTNNNNNAGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 3 cut(s) 45, 412, 443
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 3 cut(s) 42, 409, 440
BstMCI CGRYCG 1 cut(s) 412
BstMWI GCNNNNNNNGC 2 cut(s) 67, 435
BstSCI CCNGG 2 cut(s) 159, 160
BstSFI CTRYAG 2 cut(s) 59, 427
BstV1I GCAGC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
Bsu15I ATCGAT 1 cut(s) 412
BsuRI GGCC 2 cut(s) 288, 322
BsuTUI ATCGAT 1 cut(s) 412
BtgI CCRYGG 2 cut(s) 222, 381
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 260
BtsIMutI CAGTG 1 cut(s) 260
BveI ACCTGC 2 cut(s) 73, 89
Cac8I GCNNGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 321
Cfr9I CCCGGG 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 412
CviAII CATG 5 cut(s) 223, 318, 331, 367, 470
DpnI GATC 3 cut(s) 44, 411, 442
DpnII GATC 3 cut(s) 42, 409, 440
EaeI YGGCCR 1 cut(s) 286
EciI GGCGGA 1 cut(s) 4
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 222, 447
Eco24I GRGCYC 2 cut(s) 11, 160
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 2 cut(s) 160, 209
EcoICRI GAGCTC 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 222, 447
EcoT38I GRGCYC 2 cut(s) 11, 160
ErhI CCWWGG 2 cut(s) 222, 447
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 5 cut(s) 226, 321, 334, 370, 473
FaiI YATR 9 cut(s) 172, 224, 291, 310, 312, 319, 332, 368, 471
FaqI GGGAC 1 cut(s) 233
FatI CATG 5 cut(s) 222, 317, 330, 366, 469
Fnu4HI GCNGC 1 cut(s) 59
FokI GGATG 1 cut(s) 252
FriOI GRGCYC 2 cut(s) 11, 160
Fsp4HI GCNGC 1 cut(s) 59
GluI GCNGC 1 cut(s) 59
GsaI CCCAGC 2 cut(s) 329, 401
HaeIII GGCC 2 cut(s) 288, 322
HapII CCGG 4 cut(s) 95, 153, 161, 254
Hin1II CATG 5 cut(s) 226, 321, 334, 370, 473
HinfI GANTC 2 cut(s) 88, 314
HpaII CCGG 4 cut(s) 95, 153, 161, 254
HphI GGTGA 5 cut(s) 97, 109, 142, 250, 397
Hpy188I TCNGA 1 cut(s) 132
HpyCH4III ACNGT 2 cut(s) 337, 385
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 4 cut(s) 51, 61, 265, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 435
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 5 cut(s) 226, 321, 334, 370, 473
Kzo9I GATC 3 cut(s) 42, 409, 440
LmnI GCTCC 4 cut(s) 14, 155, 198, 262
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 274
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 115, 148
MalI GATC 3 cut(s) 44, 411, 442
MboI GATC 3 cut(s) 42, 409, 440
MboII GAAGA 2 cut(s) 119, 433
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 2 cut(s) 11, 160
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 1 cut(s) 183
MluNI TGGCCA 1 cut(s) 288
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 5 cut(s) 22, 102, 220, 261, 279
Mox20I TGGCCA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 4 cut(s) 95, 153, 161, 254
MspR9I CCNGG 2 cut(s) 161, 162
Mva1269I GAATGC 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 67, 435
NciI CCSGG 2 cut(s) 161, 162
NcoI CCATGG 1 cut(s) 222
NdeII GATC 3 cut(s) 42, 409, 440
NlaIII CATG 5 cut(s) 226, 321, 334, 370, 473
NlaIV GGNNCC 5 cut(s) 157, 231, 258, 323, 467
NmuCI GTSAC 2 cut(s) 115, 148
PaqCI CACCTGC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 414
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 88, 314
PkrI GCNGC 1 cut(s) 60
Ple19I CGATCG 1 cut(s) 412
Ppu21I YACGTR 1 cut(s) 418
Psp124BI GAGCTC 1 cut(s) 11
PspFI CCCAGC 2 cut(s) 325, 397
PspN4I GGNNCC 5 cut(s) 157, 231, 258, 323, 467
PspPI GGNCC 1 cut(s) 321
PstI CTGCAG 2 cut(s) 63, 431
PsuI RGATCY 1 cut(s) 440
PvuI CGATCG 1 cut(s) 412
SacI GAGCTC 1 cut(s) 11
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 3 cut(s) 42, 409, 440
Sau96I GGNCC 1 cut(s) 321
ScrFI CCNGG 2 cut(s) 161, 162
SduI GDGCHC 2 cut(s) 11, 160
SetI ASST 7 cut(s) 11, 72, 87, 103, 195, 240, 420
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 2 cut(s) 59, 427
SmaI CCCGGG 1 cut(s) 162
Sse9I AATT 1 cut(s) 183
SsiI CCGC 2 cut(s) 15, 401
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 2 cut(s) 159, 160
StyI CCWWGG 2 cut(s) 222, 447
TaaI ACNGT 2 cut(s) 337, 385
TaiI ACGT 1 cut(s) 420
TaqI TCGA 4 cut(s) 144, 201, 374, 412
TasI AATT 1 cut(s) 183
TfiI GAWTC 2 cut(s) 88, 314
TscAI CASTG 1 cut(s) 267
TseFI GTSAC 2 cut(s) 115, 148
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 2 cut(s) 115, 148
TspDTI ATGAA 1 cut(s) 306
TspMI CCCGGG 1 cut(s) 160
TspRI CASTG 1 cut(s) 267
XagI CCTNNNNNAGG 1 cut(s) 96
XapI RAATTY 1 cut(s) 183
XmaI CCCGGG 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.