pycom15g03570

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007541
Physical Location & Seq
Forward (+)
2175274 .. 2177558
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1521 bp
ATGAACCGAATTGATGAGTATATTGTGTTCCAGCCTTTGGATTCGAAAGAGATCGGTAACATTGTCGAGATACAGATGAACCGACTGAAAGACAGGCTGAAGCAGAGGAAAATTGATCTTCATTACACAAAGGAAGCCGTGGAGCTTCTCGGGACACTAGGCTTCGATCCCAACTTTGGCGCAAGGCCGGTGAAGCGGGTGATACAACAGTTGGTTGAGAATGAAATTGCAATGGGAGTCCTGAGAGGAGATTTCAACGAGGAACACTCGCTTATTGTCGATGCCGAGGCGTCCCCGTCCGCCAAACACCTTCCTCCCCAGAAGAGGTTGCGTATCAAGAAATTGGAGAGCACTTCTGCTGCAGATGCTATGGTTGTGAACGATTTCTGGGGTTACAAGGAAATAGACAAAGTTATCTTCAGTTTGAAAGTTTTGAGTTTTAACGATAAGGAAAAAATAAAAAATGGAGTGAATAGTATCATGATTGATTTTTTAACTCTTTCAATTTTCAAAAGTTTGTGTAAGGGACACTGGAGCGTAATCGAATATTTTGACAATATTGACCTTAACGAAAGGAAACCAAATCTATCTGTGTGTGTACACGAAAGACGAAATAAGCAGATGCAGGGTCGCAGGTCACATGGCAATTTCTCCACGTTAGCCTCTTACTTACGTAATTGTAGACCCCACATAAAAAATGTGTCACGCTTTCCATCTTCCCCCAGCCTGTTAGCGGATGGACATATTTGCATGGCCAACTATGGATCTCACTGTCATTGTCGTACCAATTACTACTTTTCAGATCTAATTAGTATTATGGGTACTCTTTTGGTAATTATAAATGCTAATCTTCCTCGTATTAGGCGATCTCCTGTCATATATGAGAAATCTTTGATTGAATTCAAATATTTTAGATTCATAAGAGTTGTTTGGTGCGTCAGGACAAAAGCTTATGTAAAGGCACTCGACTATGTCATCTGTAAGAAATTTAAACTTTCGAGGTATTTGGTTTTGCTCCTAAATCCGTTCATATTAAAACTCGTCAATAAGTGTACTACAACCATTGAAACAATACTTAATCTCTCACAAAAATATGAGATTTTATATATTTTAGTGTTATCTGATTGTACGCTAATTTCTCTTAGTATAAGAATTATCCTAATTAGACAAATGTACCCAATTTTTTATGTATGTTTTGGGCGTTTTCAATGGCTAATAAGTATTAACTTAATCAACTATTTTTTTAGCTCGATATGTGACAATATTATCATCACGGAACAATATGCATGCAACCCTAACATCCAGTCATTGCATCAATGTGTAAACTTAATAATAATAAAAAAGTACCTTGTGAGTCTGATTTTCTTTTACGTCGTTGACAAAATTGAAGTCATAATTTTTTTGATAAAACAAATACGTACCAAGATATGTAAGTATGCTTTTATAAGGAGCAATTTAGTTGATTTAGAGCACCCAAATTTACATTCAAGATTACAAATACCTTGTAGCTCAATTTATTAA

Protein Analysis

507

Amino Acids

59.01

Weight (kDa)

9.38

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ClpB_D2-small PF10431 13 - 91 7.9e-23 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 839, 1445
Acc36I ACCTGC 1 cut(s) 624
AccB7I CCANNNNNTGG 1 cut(s) 37
AccI GTMKAC 1 cut(s) 682
AciI CCGC 3 cut(s) 196, 300, 734
AclWI GGATC 2 cut(s) 161, 772
AcoI YGGCCR 1 cut(s) 753
AcsI RAATTY 3 cut(s) 899, 986, 1477
AcuI CTGAAG 2 cut(s) 119, 403
AcyI GRCGYC 1 cut(s) 290
AfaI GTAC 8 cut(s) 600, 784, 823, 1054, 1129, 1175, 1346, 1420
AfiI CCNNNNNNNGG 4 cut(s) 37, 176, 324, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 1468, 1500
AluBI AGCT 4 cut(s) 145, 950, 1248, 1509
AluI AGCT 4 cut(s) 145, 950, 1248, 1509
Alw21I GWGCWC 2 cut(s) 353, 1473
AlwI GGATC 2 cut(s) 161, 772
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 185, 753
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 3 cut(s) 899, 986, 1477
ArsI GACNNNNNNTTYG 2 cut(s) 1374, 1406
Asp700I GAANNNNTTC 1 cut(s) 383
AspLEI GCGC 1 cut(s) 182
AsuHPI GGTGA 2 cut(s) 202, 211
AsuII TTCGAA 1 cut(s) 44
AvaI CYCGRG 1 cut(s) 149
BaeI ACNNNNGTAYC 4 cut(s) 194, 227, 1157, 1190
BalI TGGCCA 1 cut(s) 755
Bbv12I GWGCWC 2 cut(s) 353, 1473
BbvI GCAGC 1 cut(s) 346
BccI CCATC 2 cut(s) 721, 731
BceAI ACGGC 1 cut(s) 122
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 360
BfuAI ACCTGC 1 cut(s) 624
BglII AGATCT 1 cut(s) 802
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 149
BmsI GCATC 4 cut(s) 271, 355, 612, 1321
BplI GAGNNNNNCTC 2 cut(s) 251, 283
BpmI CTGGAG 1 cut(s) 553
Bpu14I TTCGAA 1 cut(s) 44
BsaAI YACGTR 2 cut(s) 674, 1418
BsaHI GRCGYC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 138, 285
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 176, 324, 733
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 2 cut(s) 536, 1303
Bse3DI GCAATG 2 cut(s) 237, 1307
BseDI CCNNGG 2 cut(s) 138, 285
BseGI GGATG 2 cut(s) 742, 1299
BseLI CCNNNNNNNGG 4 cut(s) 37, 176, 324, 733
BseMI GCAATG 2 cut(s) 237, 1307
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 2 cut(s) 536, 1303
BseRI GAGGAG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 346
BseYI CCCAGC 1 cut(s) 722
BshFI GGCC 2 cut(s) 187, 755
BsiHKAI GWGCWC 2 cut(s) 353, 1473
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 3 cut(s) 166, 277, 540
BslI CCNNNNNNNGG 4 cut(s) 37, 176, 324, 733
BsmFI GGGAC 3 cut(s) 166, 277, 540
BsnI GGCC 2 cut(s) 187, 755
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 353, 1473
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 6 cut(s) 51, 115, 166, 764, 802, 866
BspACI CCGC 3 cut(s) 196, 300, 734
BspANI GGCC 2 cut(s) 187, 755
BspCNI CTCAG 1 cut(s) 234
BspHI TCATGA 1 cut(s) 480
BspMAI CTGCAG 1 cut(s) 364
BspMI ACCTGC 1 cut(s) 624
BspPI GGATC 2 cut(s) 161, 772
BspT104I TTCGAA 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 237, 1307
BsrFI RCCGGY 1 cut(s) 187
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 2 cut(s) 536, 1303
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 2 cut(s) 138, 285
BssMI GATC 6 cut(s) 51, 115, 166, 764, 802, 866
BssNI GRCGYC 1 cut(s) 290
Bst4CI ACNGT 2 cut(s) 210, 773
Bst6I CTCTTC 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 290
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 2 cut(s) 674, 1418
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 1288
BstDEI CTNAG 2 cut(s) 242, 1142
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 2 cut(s) 742, 1299
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 6 cut(s) 54, 118, 169, 767, 805, 869
BstMBI GATC 6 cut(s) 51, 115, 166, 764, 802, 866
BstMWI GCNNNNNNNGC 2 cut(s) 193, 365
BstNSI RCATGY 1 cut(s) 1290
BstSFI CTRYAG 1 cut(s) 360
BstSNI TACGTA 2 cut(s) 674, 1418
BstV1I GCAGC 1 cut(s) 346
BstX2I RGATCY 2 cut(s) 764, 802
BstYI RGATCY 2 cut(s) 764, 802
BsuRI GGCC 2 cut(s) 187, 755
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 2 cut(s) 742, 1299
BtsIMutI CAGTG 2 cut(s) 529, 769
BveI ACCTGC 1 cut(s) 624
Cac8I GCNNGC 1 cut(s) 1288
CciI TCATGA 1 cut(s) 480
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 187
CseI GACGC 2 cut(s) 279, 925
Csp6I GTAC 8 cut(s) 599, 783, 822, 1053, 1128, 1174, 1345, 1419
CviAII CATG 4 cut(s) 481, 641, 751, 1287
CviQI GTAC 8 cut(s) 599, 783, 822, 1053, 1128, 1174, 1345, 1419
DdeI CTNAG 2 cut(s) 242, 1142
DpnI GATC 6 cut(s) 53, 117, 168, 766, 804, 868
DpnII GATC 6 cut(s) 51, 115, 166, 764, 802, 866
DraI TTTAAA 1 cut(s) 991
EaeI YGGCCR 1 cut(s) 753
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
EciI GGCGGA 1 cut(s) 289
Eco105I TACGTA 2 cut(s) 674, 1418
Eco57I CTGAAG 2 cut(s) 119, 403
Eco88I CYCGRG 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 899
EcoT22I ATGCAT 1 cut(s) 1288
FaeI CATG 4 cut(s) 484, 644, 754, 1290
FaqI GGGAC 3 cut(s) 166, 277, 540
FatI CATG 4 cut(s) 480, 640, 750, 1286
FauI CCCGC 1 cut(s) 189
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 2 cut(s) 749, 1286
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 360
GsaI CCCAGC 1 cut(s) 726
GsuI CTGGAG 1 cut(s) 553
HaeIII GGCC 2 cut(s) 187, 755
HapII CCGG 1 cut(s) 188
HgaI GACGC 2 cut(s) 279, 925
HhaI GCGC 1 cut(s) 182
Hin1I GRCGYC 1 cut(s) 290
Hin1II CATG 4 cut(s) 484, 644, 754, 1290
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 1378
HindII GTYRAC 1 cut(s) 1378
HindIII AAGCTT 1 cut(s) 948
HinfI GANTC 4 cut(s) 41, 237, 915, 1354
HpaII CCGG 1 cut(s) 188
HphI GGTGA 2 cut(s) 202, 211
Hpy166II GTNNAC 7 cut(s) 379, 599, 601, 683, 1053, 1324, 1378
Hpy188I TCNGA 3 cut(s) 802, 1123, 1359
Hpy188III TCNNGA 7 cut(s) 67, 151, 241, 337, 481, 940, 1488
Hpy8I GTNNAC 7 cut(s) 379, 599, 601, 683, 1053, 1324, 1378
Hpy99I CGWCG 1 cut(s) 1376
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 2 cut(s) 210, 773
HpyCH4IV ACGT 4 cut(s) 656, 673, 1371, 1417
HpyCH4V TGCA 7 cut(s) 230, 362, 625, 750, 1286, 1290, 1312
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 365
HpyF3I CTNAG 2 cut(s) 242, 1142
HpySE526I ACGT 4 cut(s) 656, 673, 1371, 1417
Hsp92I GRCGYC 1 cut(s) 290
Hsp92II CATG 4 cut(s) 484, 644, 754, 1290
HspAI GCGC 1 cut(s) 180
Kzo9I GATC 6 cut(s) 51, 115, 166, 764, 802, 866
LmnI GCTCC 4 cut(s) 142, 534, 1020, 1449
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 4 cut(s) 271, 355, 612, 1321
MaeI CTAG 1 cut(s) 158
MaeII ACGT 4 cut(s) 656, 673, 1371, 1417
MaeIII GTNAC 5 cut(s) 56, 392, 636, 702, 1256
MalI GATC 6 cut(s) 53, 117, 168, 766, 804, 868
MboI GATC 6 cut(s) 51, 115, 166, 764, 802, 866
MboII GAAGA 5 cut(s) 110, 334, 409, 708, 842
MflI RGATCY 2 cut(s) 764, 802
MhlI GDGCHC 2 cut(s) 353, 1473
MlsI TGGCCA 1 cut(s) 755
MluNI TGGCCA 1 cut(s) 755
MlyI GAGTC 2 cut(s) 246, 1363
MnlI CCTC 9 cut(s) 99, 239, 253, 280, 318, 324, 673, 864, 993
Mox20I TGGCCA 1 cut(s) 755
Mph1103I ATGCAT 1 cut(s) 1288
MroXI GAANNNNTTC 1 cut(s) 383
MscI TGGCCA 1 cut(s) 755
MslI CAYNNNNRTG 1 cut(s) 1317
Msp20I TGGCCA 1 cut(s) 755
MspI CCGG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 193, 365
NdeII GATC 6 cut(s) 51, 115, 166, 764, 802, 866
NlaIII CATG 4 cut(s) 484, 644, 754, 1290
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 3 cut(s) 636, 702, 1256
NsiI ATGCAT 1 cut(s) 1288
NspI RCATGY 1 cut(s) 1290
NspV TTCGAA 1 cut(s) 44
PaeI GCATGC 1 cut(s) 1290
PagI TCATGA 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 862
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 41, 915
PflFI GACNNNGTC 1 cut(s) 971
PflMI CCANNNNNTGG 1 cut(s) 37
PkrI GCNGC 1 cut(s) 361
PleI GAGTC 2 cut(s) 245, 1362
PpsI GAGTC 2 cut(s) 245, 1362
Ppu21I YACGTR 2 cut(s) 674, 1418
PsiI TTATAA 2 cut(s) 839, 1445
PspFI CCCAGC 1 cut(s) 722
PsrI GAACNNNNNNTAC 2 cut(s) 11, 43
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 2 cut(s) 764, 802
PsyI GACNNNGTC 1 cut(s) 971
RsaI GTAC 8 cut(s) 600, 784, 823, 1054, 1129, 1175, 1346, 1420
RsaNI GTAC 8 cut(s) 599, 783, 822, 1053, 1128, 1174, 1345, 1419
RseI CAYNNNNRTG 1 cut(s) 1317
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 6 cut(s) 51, 115, 166, 764, 802, 866
SchI GAGTC 2 cut(s) 246, 1363
SduI GDGCHC 2 cut(s) 353, 1473
SfaNI GCATC 4 cut(s) 271, 355, 612, 1321
SfcI CTRYAG 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 1317
SnaBI TACGTA 2 cut(s) 674, 1418
SphI GCATGC 1 cut(s) 1290
SsiI CCGC 3 cut(s) 196, 300, 734
SspI AATATT 4 cut(s) 548, 559, 908, 1264
SspMI CTAG 1 cut(s) 158
TaaI ACNGT 2 cut(s) 210, 773
TaiI ACGT 4 cut(s) 659, 676, 1374, 1420
TaqI TCGA 8 cut(s) 44, 66, 165, 279, 543, 966, 998, 1250
TatI WGTACW 2 cut(s) 598, 1052
TfiI GAWTC 2 cut(s) 41, 915
TscAI CASTG 2 cut(s) 536, 776
TseFI GTSAC 3 cut(s) 636, 702, 1256
TseI GCWGC 1 cut(s) 359
Tsp45I GTSAC 3 cut(s) 636, 702, 1256
TspDTI ATGAA 6 cut(s) 17, 92, 110, 237, 907, 1018
TspGWI ACGGA 2 cut(s) 1014, 1289
TspRI CASTG 2 cut(s) 536, 776
Tth111I GACNNNGTC 1 cut(s) 971
Van91I CCANNNNNTGG 1 cut(s) 37
XapI RAATTY 3 cut(s) 899, 986, 1477
XceI RCATGY 1 cut(s) 1290
XmiI GTMKAC 1 cut(s) 682
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 1 cut(s) 158
Zsp2I ATGCAT 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.