Rh2CG572500

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007541
Physical Location & Seq
Forward (+)
75164565 .. 75165215
651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG572500.1

Sequence Viewer

Length: 576 bp
ATGGTTTTTGCATTTTTACAACAGTTGGAACGAGTCCAGAAGAGAACTACAGACCGCAAGATGAAAATCCAAGTGAGTGATGCAACTGTGCAGTTTCTTGGAAGTCTTGGCTACGATCCAAACTACGGTGCTAGGCCAGTGAAGCGAGTGATTCAGCAATATGTAGAAAATAAGCTTGCCAAGGGCATCTTGAGCGGAGAGTTCAAAGAAGAAGACACCATTTTGGTCGACACAGAGGTCACAACGTTTGCCAATGGCCAACTACCCCAGCAAAAGCTAGTCTTCAAGACGCATTCTGACGTGTTCAATGTTTTCCTTCAAATTTTAGATGATGGGAGATACATACTTAACGGAGATGATGAAGTATCGCCAAAGGAGTTGGGTTACGAAACTATAAAGCAGCGGGTCATGGAGCCTGCAAGGTCCATATTTCGCCCTGAGTTCATGAATCGGGTTGATGAGTACATAGTTTTTCATCCCTTGGACCGTGATCAGATAAACAACATTGTCAAGATACAGGTAATTTGCTGCTCAAAAATGCTTACTAGCTTGAAAACTTTGCTGAAAGACATTTAA

Protein Analysis

191

Amino Acids

22.09

Weight (kDa)

6.75

Isoelectric Point (pI)

32.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ClpB_D2-small PF10431 7 - 76 2.6e-22 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AccBSI CCGCTC 1 cut(s) 195
AccI GTMKAC 1 cut(s) 228
AciI CCGC 3 cut(s) 55, 195, 403
AclI AACGTT 1 cut(s) 245
AclWI GGATC 1 cut(s) 110
AcoI YGGCCR 1 cut(s) 256
AcsI RAATTY 1 cut(s) 321
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 1 cut(s) 125
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 5 cut(s) 205, 286, 307, 320, 553
AjiI CACGTC 1 cut(s) 301
AluBI AGCT 3 cut(s) 175, 277, 549
AluI AGCT 3 cut(s) 175, 277, 549
AlwI GGATC 1 cut(s) 110
AoxI GGCC 2 cut(s) 134, 256
ApeKI GCWGC 2 cut(s) 400, 528
ApoI RAATTY 1 cut(s) 321
AspS9I GGNCC 2 cut(s) 423, 484
AvaII GGWCC 2 cut(s) 423, 484
BalI TGGCCA 1 cut(s) 258
BbsI GAAGAC 2 cut(s) 219, 274
BbvI GCAGC 2 cut(s) 412, 515
BccI CCATC 1 cut(s) 326
BclI TGATCA 1 cut(s) 490
BfaI CTAG 3 cut(s) 132, 278, 546
BfmI CTRYAG 1 cut(s) 48
BisI GCNGC 2 cut(s) 401, 529
BlsI GCNGC 2 cut(s) 402, 530
Bme18I GGWCC 2 cut(s) 423, 484
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 423, 484
BmiI GGNNCC 1 cut(s) 414
BmsI GCATC 2 cut(s) 70, 195
BpiI GAAGAC 2 cut(s) 219, 274
BpuEI CTTGAG 1 cut(s) 211
BsaBI GATNNNNATC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 180, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 137
Bse8I GATNNNNATC 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 180, 480
BseGI GGATG 1 cut(s) 475
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 1 cut(s) 125
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 137
BseXI GCAGC 2 cut(s) 412, 515
BseYI CCCAGC 1 cut(s) 267
BsgI GTGCAG 1 cut(s) 110
BshFI GGCC 2 cut(s) 136, 258
BslI CCNNNNNNNGG 1 cut(s) 125
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 2 cut(s) 136, 258
Bsp143I GATC 2 cut(s) 115, 490
BspACI CCGC 3 cut(s) 55, 195, 403
BspANI GGCC 2 cut(s) 136, 258
BspCNI CTCAG 1 cut(s) 430
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 414
BspPI GGATC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 195
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 180, 480
BssMI GATC 2 cut(s) 115, 490
BssT1I CCWWGG 2 cut(s) 180, 480
Bst4CI ACNGT 4 cut(s) 24, 88, 128, 488
Bst6I CTCTTC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 177, 417
BstDEI CTNAG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 2 cut(s) 118, 493
BstMBI GATC 2 cut(s) 115, 490
BstMWI GCNNNNNNNGC 2 cut(s) 142, 192
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 2 cut(s) 412, 515
BstV2I GAAGAC 2 cut(s) 219, 274
BsuRI GGCC 2 cut(s) 136, 258
BtrI CACGTC 1 cut(s) 301
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 177, 417
CciI TCATGA 1 cut(s) 444
Cfr13I GGNCC 2 cut(s) 423, 484
CseI GACGC 1 cut(s) 298
Csp6I GTAC 1 cut(s) 463
CviAII CATG 2 cut(s) 409, 445
CviJI RGCY 7 cut(s) 111, 136, 175, 258, 277, 415, 549
CviKI_1 RGCY 7 cut(s) 111, 136, 175, 258, 277, 415, 549
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 1 cut(s) 438
DpnI GATC 2 cut(s) 117, 492
DpnII GATC 2 cut(s) 115, 490
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco130I CCWWGG 2 cut(s) 180, 480
Eco47I GGWCC 2 cut(s) 423, 484
EcoT14I CCWWGG 2 cut(s) 180, 480
ErhI CCWWGG 2 cut(s) 180, 480
FaeI CATG 2 cut(s) 412, 448
FaiI YATR 7 cut(s) 162, 344, 395, 410, 428, 446, 467
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 266, 298
FatI CATG 2 cut(s) 408, 444
FauI CCCGC 1 cut(s) 396
FbaI TGATCA 1 cut(s) 490
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 2 cut(s) 401, 529
FokI GGATG 1 cut(s) 462
Fsp4HI GCNGC 2 cut(s) 401, 529
FspBI CTAG 3 cut(s) 132, 278, 546
GluI GCNGC 2 cut(s) 401, 529
GsaI CCCAGC 1 cut(s) 271
HaeIII GGCC 2 cut(s) 136, 258
HgaI GACGC 1 cut(s) 298
Hin1II CATG 2 cut(s) 412, 448
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 3 cut(s) 33, 151, 448
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 298, 495
Hpy188III TCNNGA 5 cut(s) 37, 190, 286, 445, 511
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 1 cut(s) 326
HpyCH4III ACNGT 4 cut(s) 24, 88, 128, 488
HpyCH4IV ACGT 2 cut(s) 245, 300
HpyCH4V TGCA 4 cut(s) 11, 83, 91, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 192
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 2 cut(s) 245, 300
Hsp92II CATG 2 cut(s) 412, 448
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 2 cut(s) 115, 490
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 6 cut(s) 50, 150, 281, 429, 450, 503
Lsp1109I GCAGC 2 cut(s) 412, 515
LweI GCATC 2 cut(s) 70, 195
MaeI CTAG 3 cut(s) 132, 278, 546
MaeII ACGT 2 cut(s) 245, 300
MaeIII GTNAC 2 cut(s) 238, 383
MalI GATC 2 cut(s) 117, 492
MbiI CCGCTC 1 cut(s) 195
MboI GATC 2 cut(s) 115, 490
MboII GAAGA 4 cut(s) 52, 221, 224, 274
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 2 cut(s) 321, 522
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 1 cut(s) 42
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 1 cut(s) 229
Mox20I TGGCCA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 258
MseI TTAA 2 cut(s) 348, 574
Msp20I TGGCCA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 403
Mva1269I GAATGC 1 cut(s) 292
MwoI GCNNNNNNNGC 2 cut(s) 142, 192
NdeII GATC 2 cut(s) 115, 490
NlaIII CATG 2 cut(s) 412, 448
NlaIV GGNNCC 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 238
PagI TCATGA 1 cut(s) 444
PctI GAATGC 1 cut(s) 292
PfeI GAWTC 2 cut(s) 151, 448
PkrI GCNGC 2 cut(s) 402, 530
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 245
PspFI CCCAGC 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 2 cut(s) 423, 484
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SalI GTCGAC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 348, 574
SatI GCNGC 2 cut(s) 401, 529
Sau3AI GATC 2 cut(s) 115, 490
Sau96I GGNCC 2 cut(s) 423, 484
SchI GAGTC 1 cut(s) 42
SetI ASST 8 cut(s) 177, 240, 248, 279, 303, 425, 522, 551
SfaNI GCATC 2 cut(s) 70, 195
SfcI CTRYAG 1 cut(s) 48
SinI GGWCC 2 cut(s) 423, 484
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 2 cut(s) 321, 522
SsiI CCGC 3 cut(s) 55, 195, 403
SspMI CTAG 3 cut(s) 132, 278, 546
StyI CCWWGG 2 cut(s) 180, 480
TaaI ACNGT 4 cut(s) 24, 88, 128, 488
TaiI ACGT 2 cut(s) 248, 303
TaqI TCGA 1 cut(s) 228
TasI AATT 2 cut(s) 321, 522
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 2 cut(s) 151, 448
Tru1I TTAA 2 cut(s) 348, 574
Tru9I TTAA 2 cut(s) 348, 574
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 2 cut(s) 400, 528
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 5 cut(s) 77, 375, 433, 461, 464
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 144
VpaK11BI GGWCC 2 cut(s) 423, 484
XapI RAATTY 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 3 cut(s) 132, 278, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.