Rh2DG612200

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007541
Physical Location & Seq
Forward (+)
84422411 .. 84422746
336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG612200.1

Sequence Viewer

Length: 336 bp
ATGGTTTTCGCCTTTTTACAACAGTTGGAACGAGTCCAAAAGAGAATTGCAGACCGCAAGATGAAGATCCAAGTGAGCGAGGCAGCTGTGCAGCTTCTTGGAAATCTCGGCTACGATCCAAACTACGGTGTTAGGCCAGTCAAGCGAGTGATTCAGCAATATGTAGAAAATGAGCTTGCCAAGGGCATCTTGAGAGGAGAGTTCAAAGAAGAAGACACCATTTTGGTCGACACAGAGATCACGGCGTTTGCCAATGGCCAGCTACCCCAGCAAAAGCTATTCTTCAAGACGCTTGAAACTGGTTCAGAATCTCCTGCAGCAGAGAACTCCGTCTAA

Protein Analysis

111

Amino Acids

12.54

Weight (kDa)

5.51

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ClpB_D2-small PF10431 7 - 76 3.2e-22 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 228
AciI CCGC 1 cut(s) 55
AclWI GGATC 2 cut(s) 61, 110
AcoI YGGCCR 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 125
AgsI TTSAA 3 cut(s) 205, 286, 296
AluBI AGCT 5 cut(s) 86, 94, 175, 262, 277
AluI AGCT 5 cut(s) 86, 94, 175, 262, 277
AlwI GGATC 2 cut(s) 61, 110
AoxI GGCC 2 cut(s) 134, 256
ApeKI GCWGC 3 cut(s) 83, 91, 317
BalI TGGCCA 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 219
BbvI GCAGC 3 cut(s) 95, 103, 329
BceAI ACGGC 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 315
BisI GCNGC 3 cut(s) 84, 92, 318
BlsI GCNGC 3 cut(s) 85, 93, 319
BmsI GCATC 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 211
BsaBI GATNNNNATC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 180
Bsc4I CCNNNNNNNGG 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 137, 304
Bse8I GATNNNNATC 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 180
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 1 cut(s) 125
BseNI ACTGG 2 cut(s) 137, 304
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 3 cut(s) 95, 103, 329
BseYI CCCAGC 1 cut(s) 267
BsgI GTGCAG 1 cut(s) 110
BshFI GGCC 2 cut(s) 136, 258
BslI CCNNNNNNNGG 1 cut(s) 125
BsnI GGCC 2 cut(s) 136, 258
Bsp143I GATC 3 cut(s) 66, 115, 237
BspACI CCGC 1 cut(s) 55
BspANI GGCC 2 cut(s) 136, 258
BspMAI CTGCAG 1 cut(s) 319
BspPI GGATC 2 cut(s) 61, 110
BsrI ACTGG 2 cut(s) 137, 304
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 3 cut(s) 66, 115, 237
BssT1I CCWWGG 1 cut(s) 180
Bst4CI ACNGT 2 cut(s) 24, 128
BstC8I GCNNGC 2 cut(s) 177, 260
BstKTI GATC 3 cut(s) 69, 118, 240
BstMBI GATC 3 cut(s) 66, 115, 237
BstMWI GCNNNNNNNGC 2 cut(s) 142, 268
BstSFI CTRYAG 1 cut(s) 315
BstV1I GCAGC 3 cut(s) 95, 103, 329
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 2 cut(s) 136, 258
Cac8I GCNNGC 2 cut(s) 177, 260
CseI GACGC 1 cut(s) 298
CviJI RGCY 8 cut(s) 86, 94, 111, 136, 175, 258, 262, 277
CviKI_1 RGCY 8 cut(s) 86, 94, 111, 136, 175, 258, 262, 277
DpnI GATC 3 cut(s) 68, 117, 239
DpnII GATC 3 cut(s) 66, 115, 237
EaeI YGGCCR 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaiI YATR 1 cut(s) 162
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 266, 298
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 84, 92, 318
Fsp4HI GCNGC 3 cut(s) 84, 92, 318
GluI GCNGC 3 cut(s) 84, 92, 318
GsaI CCCAGC 1 cut(s) 271
HaeIII GGCC 2 cut(s) 136, 258
HgaI GACGC 1 cut(s) 298
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 3 cut(s) 33, 151, 308
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 2 cut(s) 190, 286
Hpy8I GTNNAC 1 cut(s) 229
HpyCH4III ACNGT 2 cut(s) 24, 128
HpyCH4V TGCA 3 cut(s) 50, 91, 317
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 268
Kzo9I GATC 3 cut(s) 66, 115, 237
LpnPI CCDG 5 cut(s) 150, 272, 281, 285, 327
Lsp1109I GCAGC 3 cut(s) 95, 103, 329
LweI GCATC 1 cut(s) 195
MalI GATC 3 cut(s) 68, 117, 239
MboI GATC 3 cut(s) 66, 115, 237
MboII GAAGA 4 cut(s) 76, 221, 224, 274
MflI RGATCY 1 cut(s) 66
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 258
MlyI GAGTC 1 cut(s) 42
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 2 cut(s) 73, 188
Mox20I TGGCCA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 258
Msp20I TGGCCA 1 cut(s) 258
MspA1I CMGCKG 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 142, 268
NdeII GATC 3 cut(s) 66, 115, 237
NmeAIII GCCGAG 1 cut(s) 87
PfeI GAWTC 2 cut(s) 151, 308
PkrI GCNGC 3 cut(s) 85, 93, 319
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PspFI CCCAGC 1 cut(s) 267
PstI CTGCAG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 86
SalI GTCGAC 1 cut(s) 227
SatI GCNGC 3 cut(s) 84, 92, 318
Sau3AI GATC 3 cut(s) 66, 115, 237
SchI GAGTC 1 cut(s) 42
SetI ASST 5 cut(s) 88, 96, 177, 264, 279
SfaNI GCATC 1 cut(s) 195
SfcI CTRYAG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 1 cut(s) 45
SsiI CCGC 1 cut(s) 55
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 2 cut(s) 24, 128
TaqI TCGA 1 cut(s) 228
TasI AATT 1 cut(s) 45
TfiI GAWTC 2 cut(s) 151, 308
TseI GCWGC 3 cut(s) 83, 91, 317
TspDTI ATGAA 1 cut(s) 77
TspGWI ACGGA 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.