pycom15g04580

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003789
Physical Location & Seq
Forward (+)
2730536 .. 2734546
4011 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1683 bp
ATGTCAGGCGACGAAAAATGGTGCGTGGTCACCGGAGGCCGAGGCTTCGCGGCGCGCCACTTGGTCCAGATGCTGATCCGATACGATGTGTTCCGGGTCAGAATCGCCGACCTCGGCTCCACCGTTGATCTAGAACCTTCCGAAGAAAACGGCATTCTAGCCGAAGCCCTGAGCACCGGTCGCGCCCAGTACGTGTCCCTCGACCTCCGCAGCAAGAGCCAAGTTCTTCAAGCGTTACAAGGCGCGGAGGTTGTGTTCCACATGGCGGCTGCAAATTCGTCCATCAACAATTACAAGCTCCACCACTCGGTCAATGTAGAAGGAACTCAGAATGTGATTGATGCTTGTACTGAGTTCAAAGTAAAGAGACTAATCTATACTAGCTCCCCGAGTGTTGTTTTCGATGGGATTCATGGGATTATTGACGGGGATGAGTCTTTGCCATATCCACCTAAGCACAATGATTTCTATTCTGCAACGAAAGCCCAAGGAGAAGAATTGGTCATCAAGGCAAATGGTGTTAAGGGGCTTCTCACCTGTTGCATTCGCCCTAGCAGCATTTTTGGACCTGGTGATAGGTTGCTGGTTCCGTCATTGGTTGTTGCCGCCAGGGCAGGGATATCCAAGTTCATCATTGGTGATGGCAACAACATTTATGATTTCACATATGTTGAAAATGTGGCGCACGCGCATATATGTGCAGAGCGAGCTCTTGCATCAGAAGGGACAGTTGCAGATAAAGCTGCAGGGCAGGCATATTTTATAACCAATATGGAACCTATCAAGTTTTGGGAGTTTGTTTCACTTATTGTAGAAGGTCTTGGCTATGAAAGGCCAAGAATAAAAATCCCTGCCTTTGTTGTGATGCCAATTGCACATATGGTGGAGTTGACATATAAGCTGTTAGGCCCATATGGGATGAAGCTTCCACAGTTGACGCCTTCGAGAATTAGACTCCTCTCTTGCACCCGATCTTTTAAGTGTTCGAAAGCACAGGATCGAATCAGCTACACACCCATCGTATCACTTCAGGATGGTCTGAAGAGGACAATTGATGCATACCCACATTTGAGGGCTGAACATCGACCGAAAAGAGAAGGACCATCTAAAGCGTCAATATATCTTGGAAGTGGAAGGGTAGCTGACACATTACTCTGGAAGGATAAAAAACAGACGTTGAAGGCGTTAATTGTTTTGATTGCAATCTACTACAACTTGATTGTACCTGAATCTACAATTATTACTATGCTTTCAAAGGTTCTCCTGGTAGCTTCAGTATTTGTGTTCACTCATGCTGGTTTACCTGAAAAGATATTAGGATACAAAATTGAAAAGCTTCAGTCATCAGATTTCCGCTTATCAGAAGAGACGTCGCAACAAATTGCCAAGTCTCTGGCTTCATTATGGAATGCATCTGTTAAAACTTTGAAATCTCTTGCCAAGGGATATGACGGGATATTGTTCCTTAAGGTTGTTCTCACCCTATTGGTTCTTAGCTTCCTTGGAGCGTTTTCACTTCAGAACTTATATCTTATAGGAGTTCCCGCAGCCTTCATAGTTTTCTACGTGTACGAGAAGGAGGAGCAAAAAATTGATGACATGGTCCTAGAAGCTCTCTCTCGTGGACGCAAATTGAAATCTGATGTTGTCAAAAAGTTCTGTACATCCAAGAAGATTGAGTGA

Protein Analysis

561

Amino Acids

61.95

Weight (kDa)

9.07

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 9 - 283 1.7e-77 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 9 - 237 2.7e-32 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 129 4e-08 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 10 - 262 2.1e-15 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 57 - 133 3.2e-08 NAD(P)H-binding
RmlD_sub_bind PF04321 58 - 281 5.9e-08 RmlD substrate binding domain
NAD_binding_4 PF07993 77 - 229 6.4e-13 Male sterility protein
Reticulon PF02453 380 - 535 6.2e-32 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 764
AatII GACGTC 1 cut(s) 1373
AccII CGCG 5 cut(s) 50, 55, 183, 245, 689
AciI CCGC 7 cut(s) 50, 208, 245, 266, 606, 1354, 1545
AclWI GGATC 2 cut(s) 70, 1005
AcsI RAATTY 1 cut(s) 274
AcuI CTGAAG 5 cut(s) 1013, 1061, 1257, 1322, 1502
AcyI GRCGYC 2 cut(s) 938, 1370
AfaI GTAC 5 cut(s) 191, 349, 1224, 1571, 1663
AfiI CCNNNNNNNGG 3 cut(s) 265, 307, 615
AflII CTTAAG 1 cut(s) 1466
AflIII ACRYGT 2 cut(s) 192, 1566
AgeI ACCGGT 1 cut(s) 176
AgsI TTSAA 8 cut(s) 230, 358, 674, 1180, 1254, 1331, 1429, 1636
AjnI CCWGG 3 cut(s) 568, 608, 1263
AloI GAACNNNNNNTCC 2 cut(s) 239, 271
Alw21I GWGCWC 2 cut(s) 176, 712
Alw26I GTCTC 3 cut(s) 361, 1361, 1395
AlwI GGATC 2 cut(s) 70, 1005
AlwNI CAGNNNCTG 1 cut(s) 73
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 3 cut(s) 37, 833, 907
ApeKI GCWGC 5 cut(s) 210, 269, 555, 743, 1547
ApoI RAATTY 1 cut(s) 274
AscI GGCGCGCC 1 cut(s) 53
AsiGI ACCGGT 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 1335
AspLEI GCGC 6 cut(s) 55, 57, 185, 245, 685, 691
AspS9I GGNCC 5 cut(s) 64, 566, 908, 1100, 1603
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 5 cut(s) 22, 526, 584, 650, 1471
AsuII TTCGAA 1 cut(s) 986
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 4 cut(s) 64, 566, 1100, 1603
BanII GRGCYC 1 cut(s) 712
BauI CACGAG 1 cut(s) 1620
Bbv12I GWGCWC 2 cut(s) 176, 712
BbvI GCAGC 5 cut(s) 222, 256, 567, 730, 1559
BccI CCATC 6 cut(s) 290, 398, 635, 1025, 1028, 1111
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 3 cut(s) 570, 610, 1265
BciVI GTATCC 1 cut(s) 1313
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 3 cut(s) 361, 1361, 1395
BfaI CTAG 5 cut(s) 131, 158, 381, 552, 1607
BfmI CTRYAG 1 cut(s) 744
BfrI CTTAAG 1 cut(s) 1466
BfuI GTATCC 1 cut(s) 1313
BglI GCCNNNNNGGC 1 cut(s) 611
BisI GCNGC 8 cut(s) 51, 211, 267, 270, 556, 606, 744, 1548
BlsI GCNGC 8 cut(s) 52, 212, 268, 271, 557, 607, 745, 1549
Bme1390I CCNGG 4 cut(s) 95, 570, 610, 1265
Bme18I GGWCC 4 cut(s) 64, 566, 1100, 1603
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 5 cut(s) 64, 566, 908, 1100, 1603
BmiI GGNNCC 3 cut(s) 118, 588, 777
BmrFI CCNGG 4 cut(s) 95, 570, 610, 1265
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 6 cut(s) 60, 331, 725, 855, 1045, 1421
BmuI ACTGGG 1 cut(s) 181
Bpu10I CCTNAGC 2 cut(s) 170, 453
Bpu14I TTCGAA 1 cut(s) 986
BpuMI CCSGG 1 cut(s) 95
BsaAI YACGTR 2 cut(s) 193, 1567
BsaBI GATNNNNATC 2 cut(s) 74, 1202
BsaHI GRCGYC 2 cut(s) 938, 1370
BsaJI CCNNGG 6 cut(s) 40, 112, 487, 609, 1440, 1501
BsaWI WCCGGW 2 cut(s) 32, 176
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 307, 615
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 187
Bse8I GATNNNNATC 2 cut(s) 74, 1202
BseBI CCWGG 3 cut(s) 570, 610, 1265
BseDI CCNNGG 6 cut(s) 40, 112, 487, 609, 1440, 1501
BseGI GGATG 4 cut(s) 436, 924, 1039, 1664
BseJI GATNNNNATC 2 cut(s) 74, 1202
BseLI CCNNNNNNNGG 3 cut(s) 265, 307, 615
BseMII CTCAG 3 cut(s) 161, 341, 342
BseNI ACTGG 1 cut(s) 187
BsePI GCGCGC 1 cut(s) 53
BseRI GAGGAG 2 cut(s) 947, 1595
BseXI GCAGC 5 cut(s) 222, 256, 567, 730, 1559
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 5 cut(s) 50, 55, 183, 245, 689
Bsh1285I CGRYCG 2 cut(s) 181, 1088
BshFI GGCC 3 cut(s) 39, 835, 909
BshTI ACCGGT 1 cut(s) 176
BsiEI CGRYCG 2 cut(s) 181, 1088
BsiHKAI GWGCWC 2 cut(s) 176, 712
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 3 cut(s) 33, 94, 177
BslFI GGGAC 2 cut(s) 181, 739
BslI CCNNNNNNNGG 3 cut(s) 265, 307, 615
BsmAI GTCTC 3 cut(s) 361, 1361, 1395
BsmBI CGTCTC 1 cut(s) 1361
BsmFI GGGAC 2 cut(s) 181, 739
BsmI GAATGC 3 cut(s) 153, 543, 1414
BsnI GGCC 3 cut(s) 39, 835, 909
BsoBI CYCGRG 1 cut(s) 388
Bsp119I TTCGAA 1 cut(s) 986
Bsp1286I GDGCHC 2 cut(s) 176, 712
Bsp1407I TGTACA 1 cut(s) 1661
Bsp143I GATC 4 cut(s) 75, 127, 971, 997
BspACI CCGC 7 cut(s) 50, 208, 245, 266, 606, 1354, 1545
BspANI GGCC 3 cut(s) 39, 835, 909
BspCNI CTCAG 3 cut(s) 162, 340, 343
BspFNI CGCG 5 cut(s) 50, 55, 183, 245, 689
BspLI GGNNCC 3 cut(s) 118, 588, 777
BspMAI CTGCAG 1 cut(s) 748
BspPI GGATC 2 cut(s) 70, 1005
BspT104I TTCGAA 1 cut(s) 986
BspTI CTTAAG 1 cut(s) 1466
BsrFI RCCGGY 1 cut(s) 176
BsrGI TGTACA 1 cut(s) 1661
BsrI ACTGG 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 6 cut(s) 40, 112, 487, 609, 1440, 1501
BssHII GCGCGC 1 cut(s) 53
BssMI GATC 4 cut(s) 75, 127, 971, 997
BssNI GRCGYC 2 cut(s) 938, 1370
BssSI CACGAG 1 cut(s) 1620
BssT1I CCWWGG 3 cut(s) 487, 1440, 1501
Bst2BI CACGAG 1 cut(s) 1620
Bst2UI CCWGG 3 cut(s) 570, 610, 1265
Bst4CI ACNGT 3 cut(s) 124, 730, 933
Bst6I CTCTTC 2 cut(s) 1037, 1359
BstACI GRCGYC 2 cut(s) 938, 1370
BstAFI CTTAAG 1 cut(s) 1466
BstAUI TGTACA 1 cut(s) 1661
BstBAI YACGTR 2 cut(s) 193, 1567
BstBI TTCGAA 1 cut(s) 986
BstC8I GCNNGC 4 cut(s) 55, 687, 708, 753
BstDEI CTNAG 5 cut(s) 170, 327, 351, 453, 1493
BstEII GGTNACC 1 cut(s) 28
BstF5I GGATG 4 cut(s) 436, 924, 1039, 1664
BstFNI CGCG 5 cut(s) 50, 55, 183, 245, 689
BstHHI GCGC 6 cut(s) 55, 57, 185, 245, 685, 691
BstKTI GATC 4 cut(s) 78, 130, 974, 1000
BstMAI GTCTC 3 cut(s) 361, 1361, 1395
BstMBI GATC 4 cut(s) 75, 127, 971, 997
BstMCI CGRYCG 2 cut(s) 181, 1088
BstMWI GCNNNNNNNGC 8 cut(s) 180, 216, 482, 555, 611, 707, 740, 752
BstNI CCWGG 3 cut(s) 570, 610, 1265
BstPI GGTNACC 1 cut(s) 28
BstSCI CCNGG 4 cut(s) 93, 568, 608, 1263
BstSFI CTRYAG 1 cut(s) 744
BstUI CGCG 5 cut(s) 50, 55, 183, 245, 689
BstV1I GCAGC 5 cut(s) 222, 256, 567, 730, 1559
BstXI CCANNNNNNTGG 1 cut(s) 1393
BsuI GTATCC 1 cut(s) 1313
BsuRI GGCC 3 cut(s) 39, 835, 909
BtsCI GGATG 4 cut(s) 436, 924, 1039, 1664
Cac8I GCNNGC 4 cut(s) 55, 687, 708, 753
CaiI CAGNNNCTG 1 cut(s) 73
CfoI GCGC 6 cut(s) 55, 57, 185, 245, 685, 691
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 5 cut(s) 64, 566, 908, 1100, 1603
CseI GACGC 3 cut(s) 946, 1101, 1635
CsiI ACCWGGT 1 cut(s) 568
Csp6I GTAC 5 cut(s) 190, 348, 1223, 1570, 1662
CspAI ACCGGT 1 cut(s) 176
CviAII CATG 4 cut(s) 262, 413, 1292, 1600
CviQI GTAC 5 cut(s) 190, 348, 1223, 1570, 1662
DdeI CTNAG 5 cut(s) 170, 327, 351, 453, 1493
DpnI GATC 4 cut(s) 77, 129, 973, 999
DpnII GATC 4 cut(s) 75, 127, 971, 997
Eam1104I CTCTTC 2 cut(s) 1037, 1359
EarI CTCTTC 2 cut(s) 1037, 1359
Ecl136II GAGCTC 1 cut(s) 710
Eco130I CCWWGG 3 cut(s) 487, 1440, 1501
Eco24I GRGCYC 1 cut(s) 712
Eco32I GATATC 1 cut(s) 621
Eco47I GGWCC 4 cut(s) 64, 566, 1100, 1603
Eco53kI GAGCTC 1 cut(s) 710
Eco57I CTGAAG 5 cut(s) 1013, 1061, 1257, 1322, 1502
Eco88I CYCGRG 1 cut(s) 388
Eco91I GGTNACC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 710
EcoO65I GGTNACC 1 cut(s) 28
EcoRII CCWGG 3 cut(s) 568, 608, 1263
EcoRV GATATC 1 cut(s) 621
EcoT14I CCWWGG 3 cut(s) 487, 1440, 1501
EcoT22I ATGCAT 2 cut(s) 1060, 1414
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 3 cut(s) 487, 1440, 1501
Esp3I CGTCTC 1 cut(s) 1361
FaeI CATG 4 cut(s) 265, 416, 1295, 1603
FaqI GGGAC 2 cut(s) 181, 739
FatI CATG 4 cut(s) 261, 412, 1291, 1599
FauI CCCGC 1 cut(s) 1552
FauNDI CATATG 3 cut(s) 667, 879, 913
Fnu4HI GCNGC 8 cut(s) 51, 211, 267, 270, 556, 606, 744, 1548
FokI GGATG 4 cut(s) 443, 931, 1046, 1651
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 8 cut(s) 51, 211, 267, 270, 556, 606, 744, 1548
FspBI CTAG 5 cut(s) 131, 158, 381, 552, 1607
GlaI GCGC 6 cut(s) 54, 56, 184, 244, 684, 690
GluI GCNGC 8 cut(s) 51, 211, 267, 270, 556, 606, 744, 1548
HaeIII GGCC 3 cut(s) 39, 835, 909
HapII CCGG 3 cut(s) 33, 94, 177
HgaI GACGC 3 cut(s) 946, 1101, 1635
HhaI GCGC 6 cut(s) 55, 57, 185, 245, 685, 691
Hin1I GRCGYC 2 cut(s) 938, 1370
Hin1II CATG 4 cut(s) 265, 416, 1295, 1603
Hin6I GCGC 6 cut(s) 53, 55, 183, 243, 683, 689
HinP1I GCGC 6 cut(s) 53, 55, 183, 243, 683, 689
HincII GTYRAC 2 cut(s) 891, 936
HindII GTYRAC 2 cut(s) 891, 936
HindIII AAGCTT 2 cut(s) 923, 1334
HinfI GANTC 6 cut(s) 102, 409, 434, 954, 1002, 1229
HpaII CCGG 3 cut(s) 33, 94, 177
HphI GGTGA 5 cut(s) 22, 526, 584, 650, 1471
Hpy166II GTNNAC 6 cut(s) 891, 936, 1287, 1301, 1570, 1625
Hpy188III TCNNGA 5 cut(s) 67, 131, 945, 1031, 1156
Hpy8I GTNNAC 6 cut(s) 891, 936, 1287, 1301, 1570, 1625
Hpy99I CGWCG 2 cut(s) 14, 1375
HpyCH4III ACNGT 3 cut(s) 124, 730, 933
HpyCH4IV ACGT 4 cut(s) 192, 1175, 1370, 1566
HpyF10VI GCNNNNNNNGC 8 cut(s) 180, 216, 482, 555, 611, 707, 740, 752
HpyF3I CTNAG 5 cut(s) 170, 327, 351, 453, 1493
HpySE526I ACGT 4 cut(s) 192, 1175, 1370, 1566
Hsp92I GRCGYC 2 cut(s) 938, 1370
Hsp92II CATG 4 cut(s) 265, 416, 1295, 1603
HspAI GCGC 6 cut(s) 53, 55, 183, 243, 683, 689
Kzo9I GATC 4 cut(s) 75, 127, 971, 997
LmnI GCTCC 5 cut(s) 122, 303, 389, 1505, 1582
Lsp1109I GCAGC 5 cut(s) 222, 256, 567, 730, 1559
LweI GCATC 6 cut(s) 60, 331, 725, 855, 1045, 1421
MabI ACCWGGT 1 cut(s) 568
MaeI CTAG 5 cut(s) 131, 158, 381, 552, 1607
MaeII ACGT 4 cut(s) 192, 1175, 1370, 1566
MaeIII GTNAC 2 cut(s) 28, 234
MalI GATC 4 cut(s) 77, 129, 973, 999
MboI GATC 4 cut(s) 75, 127, 971, 997
MboII GAAGA 5 cut(s) 155, 218, 506, 1054, 1376
MfeI CAATTG 2 cut(s) 870, 1050
MhlI GDGCHC 2 cut(s) 176, 712
MlyI GAGTC 2 cut(s) 443, 948
Mph1103I ATGCAT 2 cut(s) 1060, 1414
MroXI GAANNNNTTC 1 cut(s) 1335
MseI TTAA 5 cut(s) 522, 978, 1187, 1419, 1467
MslI CAYNNNNRTG 1 cut(s) 696
MspCI CTTAAG 1 cut(s) 1466
MspI CCGG 3 cut(s) 33, 94, 177
MspR9I CCNGG 4 cut(s) 95, 570, 610, 1265
MunI CAATTG 2 cut(s) 870, 1050
Mva1269I GAATGC 3 cut(s) 153, 543, 1414
MvaI CCWGG 3 cut(s) 570, 610, 1265
MvnI CGCG 5 cut(s) 50, 55, 183, 245, 689
MwoI GCNNNNNNNGC 8 cut(s) 180, 216, 482, 555, 611, 707, 740, 752
NciI CCSGG 1 cut(s) 95
NdeI CATATG 3 cut(s) 667, 879, 913
NdeII GATC 4 cut(s) 75, 127, 971, 997
NlaIII CATG 4 cut(s) 265, 416, 1295, 1603
NlaIV GGNNCC 3 cut(s) 118, 588, 777
NmeAIII GCCGAG 2 cut(s) 65, 93
NmuCI GTSAC 1 cut(s) 28
NsiI ATGCAT 2 cut(s) 1060, 1414
NspV TTCGAA 1 cut(s) 986
PalAI GGCGCGCC 1 cut(s) 53
PauI GCGCGC 1 cut(s) 53
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 198, 1181
PctI GAATGC 3 cut(s) 153, 543, 1414
PdmI GAANNNNTTC 1 cut(s) 1335
PfeI GAWTC 4 cut(s) 102, 409, 1002, 1229
PflFI GACNNNGTC 1 cut(s) 1601
PinAI ACCGGT 1 cut(s) 176
PkrI GCNGC 8 cut(s) 52, 212, 268, 271, 557, 607, 745, 1549
PleI GAGTC 2 cut(s) 442, 948
PpsI GAGTC 2 cut(s) 442, 948
Ppu21I YACGTR 2 cut(s) 193, 1567
PsiI TTATAA 1 cut(s) 764
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 3 cut(s) 568, 608, 1263
PspEI GGTNACC 1 cut(s) 28
PspGI CCWGG 3 cut(s) 568, 608, 1263
PspN4I GGNNCC 3 cut(s) 118, 588, 777
PspPI GGNCC 5 cut(s) 64, 566, 908, 1100, 1603
PstI CTGCAG 1 cut(s) 748
PstNI CAGNNNCTG 1 cut(s) 73
PsyI GACNNNGTC 1 cut(s) 1601
PteI GCGCGC 1 cut(s) 53
RsaI GTAC 5 cut(s) 191, 349, 1224, 1571, 1663
RsaNI GTAC 5 cut(s) 190, 348, 1223, 1570, 1662
RseI CAYNNNNRTG 1 cut(s) 696
SacI GAGCTC 1 cut(s) 712
SaqAI TTAA 5 cut(s) 522, 978, 1187, 1419, 1467
SatI GCNGC 8 cut(s) 51, 211, 267, 270, 556, 606, 744, 1548
Sau3AI GATC 4 cut(s) 75, 127, 971, 997
Sau96I GGNCC 5 cut(s) 64, 566, 908, 1100, 1603
SchI GAGTC 2 cut(s) 443, 948
ScrFI CCNGG 4 cut(s) 95, 570, 610, 1265
SduI GDGCHC 2 cut(s) 176, 712
SexAI ACCWGGT 1 cut(s) 568
SfaNI GCATC 6 cut(s) 60, 331, 725, 855, 1045, 1421
SfcI CTRYAG 1 cut(s) 744
SfuI TTCGAA 1 cut(s) 986
SgsI GGCGCGCC 1 cut(s) 53
SinI GGWCC 4 cut(s) 64, 566, 1100, 1603
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 1466
SmoI CTYRAG 1 cut(s) 1466
SsiI CCGC 7 cut(s) 50, 208, 245, 266, 606, 1354, 1545
SspMI CTAG 5 cut(s) 131, 158, 381, 552, 1607
SstI GAGCTC 1 cut(s) 712
StyD4I CCNGG 4 cut(s) 93, 568, 608, 1263
StyI CCWWGG 3 cut(s) 487, 1440, 1501
TaaI ACNGT 3 cut(s) 124, 730, 933
TaiI ACGT 4 cut(s) 195, 1178, 1373, 1569
TaqI TCGA 6 cut(s) 201, 402, 944, 986, 1000, 1084
TaqII GACCGA 2 cut(s) 298, 1102
TatI WGTACW 2 cut(s) 347, 1661
TauI GCSGC 3 cut(s) 53, 269, 608
TfiI GAWTC 4 cut(s) 102, 409, 1002, 1229
Tru1I TTAA 5 cut(s) 522, 978, 1187, 1419, 1467
Tru9I TTAA 5 cut(s) 522, 978, 1187, 1419, 1467
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 5 cut(s) 210, 269, 555, 743, 1547
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 6 cut(s) 401, 619, 843, 935, 1389, 1543
TspGWI ACGGA 1 cut(s) 579
Tth111I GACNNNGTC 1 cut(s) 1601
Vha464I CTTAAG 1 cut(s) 1466
VpaK11BI GGWCC 4 cut(s) 64, 566, 1100, 1603
XapI RAATTY 1 cut(s) 274
XbaI TCTAGA 1 cut(s) 130
XmnI GAANNNNTTC 1 cut(s) 1335
XspI CTAG 5 cut(s) 131, 158, 381, 552, 1607
ZraI GACGTC 1 cut(s) 1371
Zsp2I ATGCAT 2 cut(s) 1060, 1414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.