pycom15g05050

Pentatricopeptide repeat-containing protein At1g63400-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008873
Physical Location & Seq
Forward (+)
3023755 .. 3025383
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1224 bp
ATGCCACCTTCTACTGTGGAAGGAAGGGCTTTGAGGAAATCAATGGCTACACCAGCCAGAGCCCTAATCAACCCATCTCTCACAACTACAGATTTCAATGCGGCAGTCCGCATAAACTCCCTTCTGAAAATTCTAAACCCACAAAAACCCGACCGCTCTAATCTGGGTTTAGCGGCTCTCAACCGTTTCTCGCCTCTCCTGAAACCAAGCCTTGTAATCCACGTGATCAAATCTCAGGCCAACCCTCACCGTGCCCTCTTCTTCTTCAACTGGGCTTCAAACCCTAACCCTAATCCCAACAATTATTCCCACACTCACCATTGCTACGTGGCCATCACCGACCTCCTCCTCTCCCACTCTCTCTTCTCCACCGCTGCCTCACTCCTTCAAGAATCCAATAGACTCAATGATTTCTTGGTCAGCAGGTTTATCGCAGTTCATGGCGGACGAGGGGATATCGGAACCGCCATGGATTGGTTTCACAAGGCGAAGTTGATCGAGAACGGGAAATGCTTGTTTTCGTACAATGCGATTTTGGGTGCTCTGGTTAGGGCTGACAGGATGAGTGTAGCTAAGGAAATATATGATCAGATTGTGAAGGAAGGTATGGTGAAGCCCGATGTCTCGACTTGTAGCGTAATGATTAATGGGTTTTGCAAAATGGGTGAAATGGAGAATGCCCAGAAGGTGTTTGATGAAATGATTTGTGAGCCAGATGTGATTACTTACAGTACCATGATTCGTGGGTTTTGTAAGATGGGTGATTTTGATAGCGCAAGGAAGGTTTGTGGTCAGATGAGGGAGAAAAAGGATTGTTTGCCCAATACAGTAACATATACAATTTTGATTGATGGGTATTGCAAGAAAGGCGAGTTGGAGGAGGCGATCAAGTGTATGAATGAAATGGAGAAGCAGGGATGTGAGCCCAATCAAGTTACTTACAGTGCTCTGATTCATGGATTTTGTAAAAAGGGTGATCTTGATAGTGCAAGGAAAGTGTTTGATAAAATGATTTGTGAGCCAGATGTGATTACTTACAATACCATGATTCTTGGATTTTGTAAAAAAGGTGATCTTGATAGTGCAAGGAAAGTGTTTTGTCAGATGAGGGAGAAAAAGGATTGTTTGCCCAATACAGTAACATATTCAATTTTGATTGATGGGTATTGTAAGAAAGGCGAGTTGGAGGAGGCGATCAAGTGTATGAATGAAATGGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR_1 PPR_2 PPR_3

Protein Analysis

408

Amino Acids

45.63

Weight (kDa)

8.44

Isoelectric Point (pI)

25.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 171 - 220 2.5e-10 PPR repeat family
PPR_1 PF12854 204 - 234 1.1e-09 PPR repeat
PPR_2 PF13041 206 - 252 1.9e-15 PPR repeat family
PPR PF01535 210 - 235 4.7e-07 PPR repeat
PPR_long PF17177 223 - 336 3.8e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 236 - 267 6.5e-12 PPR repeat
PPR_3 PF13812 237 - 286 4.6e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 238 - 287 5.8e-16 PPR repeat family
PPR PF01535 241 - 269 2.5e-07 PPR repeat
PPR_1 PF12854 270 - 303 2.6e-14 PPR repeat
PPR_2 PF13041 274 - 323 1.4e-23 PPR repeat family
PPR_3 PF13812 274 - 320 3.4e-10 Pentatricopeptide repeat domain
TPR_24 PF23276 275 - 333 9.4e-06 Fungal tetratrico peptide repeats
PPR PF01535 277 - 307 7.7e-11 PPR repeat
PPR_3 PF13812 299 - 337 1.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 306 - 337 1.3e-15 PPR repeat
PPR PF01535 312 - 338 2.5e-08 PPR repeat
PPR_2 PF13041 312 - 338 2e-06 PPR repeat family
PPR_1 PF12854 339 - 370 8.8e-14 PPR repeat
PPR_3 PF13812 340 - 389 7.2e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 341 - 391 8.8e-20 PPR repeat family
PPR PF01535 344 - 372 1.1e-08 PPR repeat
PPR_1 PF12854 373 - 406 3.2e-14 PPR repeat
PPR_2 PF13041 377 - 407 5.1e-10 PPR repeat family
PPR PF01535 380 - 407 4.9e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 414
AccB7I CCANNNNNTGG 1 cut(s) 474
AccBSI CCGCTC 1 cut(s) 156
AciI CCGC 7 cut(s) 101, 109, 154, 173, 372, 444, 465
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 129
AcvI CACGTG 1 cut(s) 223
AfaI GTAC 2 cut(s) 524, 733
AfiI CCNNNNNNNGG 1 cut(s) 474
AgsI TTSAA 5 cut(s) 97, 268, 279, 389, 1149
AjuI GAANNNNNNNTTGG 4 cut(s) 857, 889, 1166, 1198
AluBI AGCT 1 cut(s) 572
AluI AGCT 1 cut(s) 572
Alw21I GWGCWC 2 cut(s) 544, 949
Alw26I GTCTC 1 cut(s) 628
AoxI GGCC 2 cut(s) 237, 330
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 1 cut(s) 129
AseI ATTAAT 1 cut(s) 645
AspLEI GCGC 1 cut(s) 776
AsuHPI GGTGA 8 cut(s) 239, 308, 328, 622, 677, 773, 986, 1082
BaeGI GKGCMC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 332
BanII GRGCYC 2 cut(s) 64, 927
BbrPI CACGTG 1 cut(s) 223
Bbv12I GWGCWC 2 cut(s) 544, 949
BbvI GCAGC 1 cut(s) 361
BccI CCATC 5 cut(s) 82, 341, 751, 845, 1154
BcgI CGANNNNNNTGC 2 cut(s) 412, 446
BclI TGATCA 2 cut(s) 225, 586
BcoDI GTCTC 1 cut(s) 628
BfmI CTRYAG 1 cut(s) 87
BfuAI ACCTGC 1 cut(s) 414
BisI GCNGC 3 cut(s) 102, 174, 375
BlsI GCNGC 3 cut(s) 103, 175, 376
BmiI GGNNCC 1 cut(s) 463
BmrI ACTGGG 1 cut(s) 280
BmuI ACTGGG 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 573
BsaAI YACGTR 2 cut(s) 223, 328
BsaJI CCNNGG 1 cut(s) 468
Bsc4I CCNNNNNNNGG 1 cut(s) 474
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 468
BseGI GGATG 2 cut(s) 567, 923
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 4 cut(s) 335, 338, 893, 1202
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 1 cut(s) 361
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 239, 332
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 544, 949
BslI CCNNNNNNNGG 1 cut(s) 474
BsmAI GTCTC 1 cut(s) 628
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 2 cut(s) 239, 332
Bsp1286I GDGCHC 5 cut(s) 64, 256, 544, 927, 949
Bsp143I GATC 7 cut(s) 225, 495, 586, 885, 976, 1072, 1194
Bsp19I CCATGG 1 cut(s) 468
BspACI CCGC 7 cut(s) 101, 109, 154, 173, 372, 444, 465
BspANI GGCC 2 cut(s) 239, 332
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 463
BspMI ACCTGC 1 cut(s) 414
BsrBI CCGCTC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 319
BsrI ACTGG 1 cut(s) 275
BssECI CCNNGG 1 cut(s) 468
BssMI GATC 7 cut(s) 225, 495, 586, 885, 976, 1072, 1194
BssT1I CCWWGG 1 cut(s) 468
Bst4CI ACNGT 7 cut(s) 16, 185, 251, 731, 829, 944, 1138
Bst6I CTCTTC 2 cut(s) 263, 368
BstBAI YACGTR 2 cut(s) 223, 328
BstDEI CTNAG 2 cut(s) 234, 573
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 2 cut(s) 567, 923
BstHHI GCGC 1 cut(s) 776
BstKTI GATC 7 cut(s) 228, 498, 589, 888, 979, 1075, 1197
BstMAI GTCTC 1 cut(s) 628
BstMBI GATC 7 cut(s) 225, 495, 586, 885, 976, 1072, 1194
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 53, 867
BstSFI CTRYAG 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 1 cut(s) 361
BsuRI GGCC 2 cut(s) 239, 332
BtgI CCRYGG 1 cut(s) 468
BtsCI GGATG 2 cut(s) 567, 923
BtsIMutI CAGTG 1 cut(s) 949
BveI ACCTGC 1 cut(s) 414
CfoI GCGC 1 cut(s) 776
Csp6I GTAC 2 cut(s) 523, 732
CviAII CATG 5 cut(s) 440, 469, 736, 956, 1045
CviQI GTAC 2 cut(s) 523, 732
DdeI CTNAG 2 cut(s) 234, 573
DpnI GATC 7 cut(s) 227, 497, 588, 887, 978, 1074, 1196
DpnII GATC 7 cut(s) 225, 495, 586, 885, 976, 1072, 1194
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 2 cut(s) 263, 368
EarI CTCTTC 2 cut(s) 263, 368
EciI GGCGGA 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 468
Eco24I GRGCYC 2 cut(s) 64, 927
Eco32I GATATC 1 cut(s) 457
Eco72I CACGTG 1 cut(s) 223
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 468
EcoT38I GRGCYC 2 cut(s) 64, 927
ErhI CCWWGG 1 cut(s) 468
FaeI CATG 5 cut(s) 443, 472, 739, 959, 1048
FalI AAGNNNNNCTT 2 cut(s) 1059, 1091
FatI CATG 5 cut(s) 439, 468, 735, 955, 1044
FbaI TGATCA 2 cut(s) 225, 586
Fnu4HI GCNGC 3 cut(s) 102, 174, 375
FokI GGATG 2 cut(s) 574, 930
FriOI GRGCYC 2 cut(s) 64, 927
Fsp4HI GCNGC 3 cut(s) 102, 174, 375
GlaI GCGC 1 cut(s) 775
GluI GCNGC 3 cut(s) 102, 174, 375
HaeIII GGCC 2 cut(s) 239, 332
HhaI GCGC 1 cut(s) 776
Hin1II CATG 5 cut(s) 443, 472, 739, 959, 1048
Hin6I GCGC 1 cut(s) 774
HinP1I GCGC 1 cut(s) 774
HinfI GANTC 5 cut(s) 392, 402, 739, 952, 1048
HphI GGTGA 8 cut(s) 239, 308, 328, 622, 677, 773, 986, 1082
Hpy188I TCNGA 6 cut(s) 126, 461, 591, 795, 951, 1104
Hpy188III TCNNGA 6 cut(s) 199, 389, 499, 625, 980, 1076
HpyAV CCTTC 9 cut(s) 14, 18, 18, 131, 395, 592, 596, 679, 775
HpyCH4III ACNGT 7 cut(s) 16, 185, 251, 731, 829, 944, 1138
HpyCH4IV ACGT 2 cut(s) 222, 327
HpyCH4V TGCA 4 cut(s) 657, 861, 989, 1085
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 867
HpyF3I CTNAG 2 cut(s) 234, 573
HpySE526I ACGT 2 cut(s) 222, 327
Hsp92II CATG 5 cut(s) 443, 472, 739, 959, 1048
HspAI GCGC 1 cut(s) 774
Ksp22I TGATCA 2 cut(s) 225, 586
Kzo9I GATC 7 cut(s) 225, 495, 586, 885, 976, 1072, 1194
Lsp1109I GCAGC 1 cut(s) 361
MaeII ACGT 2 cut(s) 222, 327
MaeIII GTNAC 3 cut(s) 829, 934, 1138
MalI GATC 7 cut(s) 227, 497, 588, 887, 978, 1074, 1196
MbiI CCGCTC 1 cut(s) 156
MboI GATC 7 cut(s) 225, 495, 586, 885, 976, 1072, 1194
MboII GAAGA 4 cut(s) 250, 253, 256, 355
MhlI GDGCHC 5 cut(s) 64, 256, 544, 927, 949
MlsI TGGCCA 1 cut(s) 332
MluCI AATT 4 cut(s) 129, 301, 840, 1149
MluNI TGGCCA 1 cut(s) 332
MlyI GAGTC 1 cut(s) 396
MmeI TCCRAC 2 cut(s) 855, 1164
Mox20I TGGCCA 1 cut(s) 332
MscI TGGCCA 1 cut(s) 332
MseI TTAA 1 cut(s) 645
Msp20I TGGCCA 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 374
Mva1269I GAATGC 1 cut(s) 682
MwoI GCNNNNNNNGC 2 cut(s) 53, 867
NcoI CCATGG 1 cut(s) 468
NdeII GATC 7 cut(s) 225, 495, 586, 885, 976, 1072, 1194
NlaIII CATG 5 cut(s) 443, 472, 739, 959, 1048
NlaIV GGNNCC 1 cut(s) 463
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PctI GAATGC 1 cut(s) 682
PfeI GAWTC 4 cut(s) 392, 739, 952, 1048
PflMI CCANNNNNTGG 1 cut(s) 474
PkrI GCNGC 3 cut(s) 103, 175, 376
PleI GAGTC 1 cut(s) 396
PmaCI CACGTG 1 cut(s) 223
PmlI CACGTG 1 cut(s) 223
PpsI GAGTC 1 cut(s) 396
Ppu21I YACGTR 2 cut(s) 223, 328
PshBI ATTAAT 1 cut(s) 645
PspCI CACGTG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 463
RsaI GTAC 2 cut(s) 524, 733
RsaNI GTAC 2 cut(s) 523, 732
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 3 cut(s) 102, 174, 375
Sau3AI GATC 7 cut(s) 225, 495, 586, 885, 976, 1072, 1194
SchI GAGTC 1 cut(s) 396
SduI GDGCHC 5 cut(s) 64, 256, 544, 927, 949
SfcI CTRYAG 1 cut(s) 87
Sse9I AATT 4 cut(s) 129, 301, 840, 1149
SsiI CCGC 7 cut(s) 101, 109, 154, 173, 372, 444, 465
StyI CCWWGG 1 cut(s) 468
TaaI ACNGT 7 cut(s) 16, 185, 251, 731, 829, 944, 1138
TaiI ACGT 2 cut(s) 225, 330
TaqI TCGA 2 cut(s) 498, 626
TasI AATT 4 cut(s) 129, 301, 840, 1149
TauI GCSGC 2 cut(s) 104, 176
TfiI GAWTC 4 cut(s) 392, 739, 952, 1048
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 1 cut(s) 949
TseI GCWGC 1 cut(s) 374
TspDTI ATGAA 7 cut(s) 428, 711, 911, 915, 944, 1220, 1224
TspRI CASTG 1 cut(s) 949
Van91I CCANNNNNTGG 1 cut(s) 474
VspI ATTAAT 1 cut(s) 645
XapI RAATTY 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.