Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0002433

Physical Location & Seq

Genomic Coordinates Reverse (-)

11509170 .. 11515137

Gene Structure 5968 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGGCGAGGGGGACTCTGGTTAGTGGATCCATGGCGAAGGTCAAGGTCACCTTGGAGGTCGGAAACGACGGCGTTGCAGTTATCACCATCTTCAATCCCCCTGTTAACGCCTTGGCCATACCCATTATAGCTGGCTTGAATGAGAAGTTCGAGGAGGCTGGGAGGCGAAACGACGTGAAAGCTATCGTTTTGACTGGCAGTGGAGGAAGGTTTTCGGGTGGTTTCGATATCAATGTTTTCGAGAAGGTTCACGAGACTGGTATGATTGCAATGAACTATGTAGATATCTCTCCATTTGTAGCATTGAGTTACTTGGGGTGCCACGCACGTATTGCTGCTCCAAAAACACAACTTGGCCTTCCGGAGTTGTCACTTGGAGTGATTCCTGGATTTGGAGGCACACAACGTCTTCCAAGGCTTGTAGGGCTTCAGAAGGCAATTGAATTGATGCTGTCATCAAAGACAATCCTGTCTGAAGAAGGGGAGAAGCTGGGTCTTATAGATGCTATTGTGTCTCCTCAAGAGTTGCTGAAAGTATCTCGCTCTTGGGCTTTGGAAATTGCAGATAGGCGCAAACCGTGGTTGCGCTCTCTTCACAGGACAGATAAAATTGGATCCCTGTCTGAGGCACGAGAGATACTGAAAGTTGCCCGACAACAAGCCAAGAAGACTGCTCCCAATATGCCTCAGCACCAAGCATGCCTCAATGTGATTGAGGATGGCATTATTCATGGAGGATACAGTGGTGTTTTAACGGAGACTAAAGTTTTTAGGGAGTTAGTTCTAGCGGACACTTCAAAAGGTCTTGTCCATGTATTTTTTGCTCAACGCGCAACATCTAAGGTGCCCAACGTTACTGATGTTGGCCTTAAACCAAGGCAAATAAAGAAAGTTGCTGTTATTGGTGGAGGTCTGATGGGTTCTGGCATTGCTACGGCCCTTCTTGTGAGCAATATATCTGTGGTTCTAAAGGAAGTCAACTCTGAATATCTTCAGAAGGGCATAAAATCGATAGAAGGAAATGTTAAAGGCTTAGTAACTAGAAGGAGGCTGACACAGGACAAGGCACAAAAGGCTCTCTCATTGCTGAAAGGTAGTTTGGACTACTCCGACTTCAAAGATGCGGACATGGTCATAGAGGCTGTCATTGAGAGTGTTCCTCTCAAACAGAAAATTTTTAGTGAACTCGAGAAAGTTTGCCCATCTCACTGCGTCTTGGCTACAAATACATCCACTATTGACCTCAATGTTGTTGGGGAAAAGACAAACTCCCATGATCGCATTGTGGGGGCACATTTTTTCAGTCCTGCTCATGTGATGCCTCTTCTTGAAATAGTACGGACAGAGAAGACTTCGGCACAAGTAATTCTTGATCTTTTGACTGTTGGAAAAATAATAAAGAAAGTTCCAGTTGTGGTTGGTAACTGCACAGGCTTTGCAGTCAATCGAGCATTCTTTCCTTACACACAAGGTGCCCATATATTGGTCAATTCAGGTGTGGATGTATTCAGAATTGACAGGGTCATCAGCGATTTTGGCCTACCTATGGGTCCTTTCCAGCTTCAGGACTTGGCAGGCTATGGAGTAGCCCTTGCAGTTGGAAAGGAATTTTCTGCTGCTTTCCCTGATCGCACATTTAGATCGCCATTGGTTGAGATCTTGATAAAAAATGGACGAAATGGTAAAAACAATGGAAAAGGATATTACATTTATGAGAAGGGTAGCAAGCCAAAACCTGATCCTTCTGTCCTATCACTTATTGAGGAATCTCGACGAGTTACCAACATCATGCCTGGTGGAAAGCCTTTAACTGTTACAGACCAAGATATTCTGGAGGTGATACTGTTTCCTGTAGTAAATGAGGCATGCCGTATTCTAGACGAAGGAATAGTTATTCGAGCAGCAGACCTTGACATCGCATCTGTTCTTGGGATGAGTTTCCCATCTTACCGCGGGGGTATTGTCTTTTGGGCAGATATGGTCGGGCCGAAGCATATATACGCTAGTCTCAAGAAATGGTCCGGAGTATATGGCGACTTTTTCAAACCATCAAGGTTTTTGGAAGAAAGAGCAACAAGAGGGTTGCCGCTGAGTGCACCCGTATCGTCATCTGCATCTTCAAGGTCACGCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003857 GO:0004165 GO:0004300 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005911 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008017 GO:0008092 GO:0008150 GO:0008152 GO:0008692 GO:0009056 GO:0009058 GO:0009062 GO:0009506 GO:0009507 GO:0009536 GO:0009694 GO:0009695 GO:0009791 GO:0009908 GO:0009987 GO:0010817 GO:0015631 GO:0016042 GO:0016053 GO:0016054 GO:0016491 GO:0016614 GO:0016616 GO:0016829 GO:0016835 GO:0016836 GO:0016853 GO:0016854 GO:0016856 GO:0016860 GO:0016863 GO:0018812 GO:0019395 GO:0019752 GO:0022414 GO:0030054 GO:0030258 GO:0030312 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0072329 GO:0072330 GO:0090567 GO:0097159 GO:1901363 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01100 ko01110 ko01212 map00071 map00592 map01100 map01110 map01212

Pfam Domains

Protein Families

3HCDH 3HCDH_N ECH_1

Protein Analysis

712

Amino Acids

77.03

Weight (kDa)

9.41

Isoelectric Point (pI)

37.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	22 - 80	4.6e-08	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	106 - 198	8.7e-15	Enoyl-CoA hydratase/isomerase
3HCDH_N	PF02737	297 - 475	4.5e-58	3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
3HCDH	PF00725	478 - 571	2.6e-21	3-hydroxyacyl-CoA dehydrogenase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002433)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29010
fragaria_vesca	FvH4_1g04350 FvH4_1g04370
malus_domestica	MD02G1043200.v1.1 MD02G1043300.v1.1 MD02G1043500.v1.1 MD15G1181900.v1.1
prunus_persica	Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233500_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1 Prupe.7G233700_v2.0.a1
pyrus_communis	pycom02g03600 pycom02g03620 pycom15g16410
rosa_chinensis	RchiOBHm_Chr2g0090001 RchiOBHm_Chr2g0090021
rosa_laevigata	RLG00000016068 RLG00000016069 RLG00000016072 RLG00000016076
rosa_multiflora	Rmu_sc0000226.1_g000018 Rmu_sc0024500.1_g000001
rosa_roxburghii	Rroxscaffold_2G00151470
rosa_rugosa	Rorug02G0004600 Rorug02G0004600 Rorug02G0004700
rosa_samantha	Rh2AG049800 Rh2AG049900 Rh2BG048500 Rh2BG048700 Rh2CG050700 Rh2CG050900 Rh2DG050000 Rh2DG050100 Rh5DG028300
rosa_wichuraiana	Rw2G004050 Rw2G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	469
AccB1I	GGYRCC	3 cut(s)	318, 844, 1472
AccB7I	CCANNNNNTGG	1 cut(s)	1483
AccII	CGCG	2 cut(s)	831, 1953
AccIII	TCCGGA	2 cut(s)	361, 2021
AciI	CCGC	5 cut(s)	788, 1124, 1951, 1953, 2087
AclI	AACGTT	1 cut(s)	852
AclWI	GGATC	5 cut(s)	21, 34, 609, 622, 1733
AcoI	YGGCCR	1 cut(s)	114
AcsI	RAATTY	2 cut(s)	1173, 1607
AcuI	CTGAAG	4 cut(s)	413, 495, 977, 1547
AdeI	CACNNNGTG	1 cut(s)	1472
AfaI	GTAC	1 cut(s)	1338
AfiI	CCNNNNNNNGG	5 cut(s)	392, 625, 1483, 1546, 1564
AgsI	TTSAA	8 cut(s)	94, 139, 443, 798, 1117, 1331, 2044, 2121
AjiI	CACGTC	1 cut(s)	175
AjnI	CCWGG	2 cut(s)	385, 1792
AjuI	GAANNNNNNNTTGG	2 cut(s)	1082, 1114
AluBI	AGCT	4 cut(s)	131, 182, 490, 1561
AluI	AGCT	4 cut(s)	131, 182, 490, 1561
Alw21I	GWGCWC	1 cut(s)	2098
Alw26I	GTCTC	4 cut(s)	248, 519, 752, 2012
Alw44I	GTGCAC	1 cut(s)	2094
AlwI	GGATC	5 cut(s)	21, 34, 609, 622, 1733
Ama87I	CYCGRG	1 cut(s)	1187
Aor13HI	TCCGGA	2 cut(s)	361, 2021
AoxI	GGCC	6 cut(s)	114, 355, 865, 936, 1537, 1985
ApaLI	GTGCAC	1 cut(s)	2094
ApeKI	GCWGC	3 cut(s)	335, 1616, 1901
ApoI	RAATTY	2 cut(s)	1173, 1607
Asp700I	GAANNNNTTC	2 cut(s)	211, 990
AspLEI	GCGC	3 cut(s)	573, 588, 833
AspS9I	GGNCC	4 cut(s)	937, 1550, 1985, 2019
AsuHPI	GGTGA	3 cut(s)	40, 76, 1849
AvaI	CYCGRG	1 cut(s)	1187
AvaII	GGWCC	2 cut(s)	1550, 2019
BaeGI	GKGCMC	4 cut(s)	849, 1294, 1477, 2098
BaeI	ACNNNNGTAYC	2 cut(s)	730, 763
BalI	TGGCCA	1 cut(s)	116
BamHI	GGATCC	2 cut(s)	26, 614
BanI	GGYRCC	3 cut(s)	318, 844, 1472
BarI	GAAGNNNNNNTAC	2 cut(s)	1982, 2014
BauI	CACGAG	2 cut(s)	251, 630
BbsI	GAAGAC	3 cut(s)	401, 674, 1355
Bbv12I	GWGCWC	1 cut(s)	2098
BbvCI	CCTCAGC	1 cut(s)	687
BbvI	GCAGC	3 cut(s)	322, 1603, 1913
BccI	CCATC	6 cut(s)	95, 713, 910, 1210, 1951, 2056
BceAI	ACGGC	3 cut(s)	85, 951, 1854
BcgI	CGANNNNNNTGC	4 cut(s)	56, 90, 2085, 2119
BciT130I	CCWGG	2 cut(s)	387, 1794
BciVI	GTATCC	1 cut(s)	731
BcoDI	GTCTC	4 cut(s)	248, 519, 752, 2012
BfaI	CTAG	4 cut(s)	785, 1041, 1877, 2004
BfmI	CTRYAG	1 cut(s)	1851
BfuI	GTATCC	1 cut(s)	731
BglII	AGATCT	1 cut(s)	1656
BisI	GCNGC	4 cut(s)	336, 1617, 1902, 2087
BlsI	GCNGC	4 cut(s)	337, 1618, 1903, 2088
Bme1390I	CCNGG	2 cut(s)	387, 1794
Bme18I	GGWCC	2 cut(s)	1550, 2019
BmeT110I	CYCGRG	1 cut(s)	1187
BmgBI	CACGTC	1 cut(s)	175
BmgT120I	GGNCC	4 cut(s)	937, 1550, 1985, 2019
BmiI	GGNNCC	6 cut(s)	28, 320, 616, 846, 1474, 1551
BmrFI	CCNGG	2 cut(s)	387, 1794
BmsI	GCATC	6 cut(s)	438, 493, 1111, 1308, 1928, 2123
BpiI	GAAGAC	3 cut(s)	401, 674, 1355
BplI	GAGNNNNNCTC	3 cut(s)	30, 1144, 1176
BpmI	CTGGAG	1 cut(s)	1853
Bpu10I	CCTNAGC	1 cut(s)	687
BpuEI	CTTGAG	2 cut(s)	504, 1994
Bsa29I	ATCGAT	1 cut(s)	1010
BsaAI	YACGTR	1 cut(s)	329
BsaJI	CCNNGG	7 cut(s)	30, 51, 111, 413, 578, 875, 1951
BsaWI	WCCGGW	2 cut(s)	361, 2021
Bsc4I	CCNNNNNNNGG	5 cut(s)	392, 625, 1483, 1546, 1564
Bse1I	ACTGG	3 cut(s)	199, 262, 1409
Bse3DI	GCAATG	3 cut(s)	276, 927, 1082
BseAI	TCCGGA	2 cut(s)	361, 2021
BseBI	CCWGG	2 cut(s)	387, 1794
BseCI	ATCGAT	1 cut(s)	1010
BseDI	CCNNGG	7 cut(s)	30, 51, 111, 413, 578, 875, 1951
BseGI	GGATG	4 cut(s)	724, 1229, 1507, 1938
BseLI	CCNNNNNNNGG	5 cut(s)	392, 625, 1483, 1546, 1564
BseMI	GCAATG	3 cut(s)	276, 927, 1082
BseMII	CTCAG	3 cut(s)	615, 701, 2081
BseNI	ACTGG	3 cut(s)	199, 262, 1409
BseRI	GAGGAG	2 cut(s)	167, 507
BseSI	GKGCMC	4 cut(s)	849, 1294, 1477, 2098
BseXI	GCAGC	3 cut(s)	322, 1603, 1913
BseYI	CCCAGC	2 cut(s)	158, 490
BsgI	GTGCAG	1 cut(s)	1411
Bsh1236I	CGCG	2 cut(s)	831, 1953
BshFI	GGCC	6 cut(s)	116, 357, 867, 938, 1539, 1987
BshNI	GGYRCC	3 cut(s)	318, 844, 1472
BshVI	ATCGAT	1 cut(s)	1010
BsiHKAI	GWGCWC	1 cut(s)	2098
BsiHKCI	CYCGRG	1 cut(s)	1187
BsiSI	CCGG	2 cut(s)	362, 2022
BslFI	GGGAC	1 cut(s)	25
BslI	CCNNNNNNNGG	5 cut(s)	392, 625, 1483, 1546, 1564
BsmAI	GTCTC	4 cut(s)	248, 519, 752, 2012
BsmFI	GGGAC	1 cut(s)	25
BsmI	GAATGC	1 cut(s)	1451
BsnI	GGCC	6 cut(s)	116, 357, 867, 938, 1539, 1987
BsoBI	CYCGRG	1 cut(s)	1187
Bsp1286I	GDGCHC	4 cut(s)	849, 1294, 1477, 2098
Bsp13I	TCCGGA	2 cut(s)	361, 2021
Bsp143I	GATC	8 cut(s)	26, 614, 1276, 1372, 1627, 1640, 1656, 1738
Bsp19I	CCATGG	1 cut(s)	30
BspACI	CCGC	5 cut(s)	788, 1124, 1951, 1953, 2087
BspANI	GGCC	6 cut(s)	116, 357, 867, 938, 1539, 1987
BspCNI	CTCAG	3 cut(s)	616, 700, 2082
BspDI	ATCGAT	1 cut(s)	1010
BspEI	TCCGGA	2 cut(s)	361, 2021
BspFNI	CGCG	2 cut(s)	831, 1953
BspLI	GGNNCC	6 cut(s)	28, 320, 616, 846, 1474, 1551
BspPI	GGATC	5 cut(s)	21, 34, 609, 622, 1733
BspT107I	GGYRCC	3 cut(s)	318, 844, 1472
BsrDI	GCAATG	3 cut(s)	276, 927, 1082
BsrI	ACTGG	3 cut(s)	199, 262, 1409
BssECI	CCNNGG	7 cut(s)	30, 51, 111, 413, 578, 875, 1951
BssMI	GATC	8 cut(s)	26, 614, 1276, 1372, 1627, 1640, 1656, 1738
BssSI	CACGAG	2 cut(s)	251, 630
BssT1I	CCWWGG	5 cut(s)	30, 51, 111, 413, 875
Bst2BI	CACGAG	2 cut(s)	251, 630
Bst2UI	CCWGG	2 cut(s)	387, 1794
Bst4CI	ACNGT	5 cut(s)	579, 743, 1384, 1813, 1845
Bst6I	CTCTTC	2 cut(s)	597, 1329
BstAPI	GCANNNNNTGC	1 cut(s)	332
BstBAI	YACGTR	1 cut(s)	329
BstC8I	GCNNGC	5 cut(s)	133, 700, 1576, 1727, 1867
BstDEI	CTNAG	5 cut(s)	624, 687, 840, 1033, 2090
BstDSI	CCRYGG	3 cut(s)	30, 578, 1951
BstEII	GGTNACC	1 cut(s)	46
BstENI	CCTNNNNNAGG	1 cut(s)	623
BstF5I	GGATG	4 cut(s)	724, 1229, 1507, 1938
BstFNI	CGCG	2 cut(s)	831, 1953
BstHHI	GCGC	3 cut(s)	573, 588, 833
BstKTI	GATC	8 cut(s)	29, 617, 1279, 1375, 1630, 1643, 1659, 1741
BstMAI	GTCTC	4 cut(s)	248, 519, 752, 2012
BstMBI	GATC	8 cut(s)	26, 614, 1276, 1372, 1627, 1640, 1656, 1738
BstMWI	GCNNNNNNNGC	5 cut(s)	332, 424, 830, 1073, 1536
BstNI	CCWGG	2 cut(s)	387, 1794
BstNSI	RCATGY	3 cut(s)	702, 1869, 2134
BstPI	GGTNACC	1 cut(s)	46
BstSCI	CCNGG	2 cut(s)	385, 1792
BstSFI	CTRYAG	1 cut(s)	1851
BstSLI	GKGCMC	4 cut(s)	849, 1294, 1477, 2098
BstUI	CGCG	2 cut(s)	831, 1953
BstV1I	GCAGC	3 cut(s)	322, 1603, 1913
BstV2I	GAAGAC	3 cut(s)	401, 674, 1355
BstX2I	RGATCY	3 cut(s)	26, 614, 1656
BstYI	RGATCY	3 cut(s)	26, 614, 1656
Bsu15I	ATCGAT	1 cut(s)	1010
BsuI	GTATCC	1 cut(s)	731
BsuRI	GGCC	6 cut(s)	116, 357, 867, 938, 1539, 1987
BsuTUI	ATCGAT	1 cut(s)	1010
BtgI	CCRYGG	3 cut(s)	30, 578, 1951
BtgZI	GCGATG	1 cut(s)	1900
BtrI	CACGTC	1 cut(s)	175
BtsCI	GGATG	4 cut(s)	724, 1229, 1507, 1938
BtsI	GCAGTG	2 cut(s)	205, 1207
BtsIMutI	CAGTG	3 cut(s)	205, 748, 1207
Cac8I	GCNNGC	5 cut(s)	133, 700, 1576, 1727, 1867
CfoI	GCGC	3 cut(s)	573, 588, 833
Cfr13I	GGNCC	4 cut(s)	937, 1550, 1985, 2019
Cfr42I	CCGCGG	1 cut(s)	1954
ClaI	ATCGAT	1 cut(s)	1010
CseI	GACGC	1 cut(s)	1201
Csp6I	GTAC	1 cut(s)	1337
CviQI	GTAC	1 cut(s)	1337
DdeI	CTNAG	5 cut(s)	624, 687, 840, 1033, 2090
DpnI	GATC	8 cut(s)	28, 616, 1278, 1374, 1629, 1642, 1658, 1740
DpnII	GATC	8 cut(s)	26, 614, 1276, 1372, 1627, 1640, 1656, 1738
DraIII	CACNNNGTG	1 cut(s)	1472
DrdI	GACNNNNNNGTC	1 cut(s)	469
DseDI	GACNNNNNNGTC	1 cut(s)	469
EaeI	YGGCCR	1 cut(s)	114
Eam1104I	CTCTTC	2 cut(s)	597, 1329
EarI	CTCTTC	2 cut(s)	597, 1329
Eco130I	CCWWGG	5 cut(s)	30, 51, 111, 413, 875
Eco32I	GATATC	2 cut(s)	229, 286
Eco47I	GGWCC	2 cut(s)	1550, 2019
Eco57I	CTGAAG	4 cut(s)	413, 495, 977, 1547
Eco88I	CYCGRG	1 cut(s)	1187
Eco91I	GGTNACC	1 cut(s)	46
EcoNI	CCTNNNNNAGG	1 cut(s)	623
EcoO109I	RGGNCCY	1 cut(s)	1550
EcoO65I	GGTNACC	1 cut(s)	46
EcoRII	CCWGG	2 cut(s)	385, 1792
EcoRV	GATATC	2 cut(s)	229, 286
EcoT14I	CCWWGG	5 cut(s)	30, 51, 111, 413, 875
ErhI	CCWWGG	5 cut(s)	30, 51, 111, 413, 875
FalI	AAGNNNNNCTT	4 cut(s)	35, 67, 1353, 1385
FaqI	GGGAC	1 cut(s)	25
FauI	CCCGC	1 cut(s)	1946
Fnu4HI	GCNGC	4 cut(s)	336, 1617, 1902, 2087
FokI	GGATG	4 cut(s)	731, 1216, 1514, 1945
Fsp4HI	GCNGC	4 cut(s)	336, 1617, 1902, 2087
FspBI	CTAG	4 cut(s)	785, 1041, 1877, 2004
GlaI	GCGC	3 cut(s)	572, 587, 832
GluI	GCNGC	4 cut(s)	336, 1617, 1902, 2087
GsaI	CCCAGC	2 cut(s)	162, 494
GsuI	CTGGAG	1 cut(s)	1853
HaeIII	GGCC	6 cut(s)	116, 357, 867, 938, 1539, 1987
HapII	CCGG	2 cut(s)	362, 2022
HgaI	GACGC	1 cut(s)	1201
HhaI	GCGC	3 cut(s)	573, 588, 833
Hin6I	GCGC	3 cut(s)	571, 586, 831
HinP1I	GCGC	3 cut(s)	571, 586, 831
HincII	GTYRAC	2 cut(s)	106, 979
HindII	GTYRAC	2 cut(s)	106, 979
HinfI	GANTC	3 cut(s)	13, 382, 1766
HpaI	GTTAAC	1 cut(s)	106
HpaII	CCGG	2 cut(s)	362, 2022
HphI	GGTGA	3 cut(s)	40, 76, 1849
Hpy166II	GTNNAC	5 cut(s)	106, 250, 979, 1184, 2096
Hpy188I	TCNGA	9 cut(s)	62, 432, 475, 625, 915, 985, 996, 1111, 1511
Hpy8I	GTNNAC	5 cut(s)	106, 250, 979, 1184, 2096
Hpy99I	CGWCG	3 cut(s)	71, 176, 1776
HpyCH4III	ACNGT	5 cut(s)	579, 743, 1384, 1813, 1845
HpyCH4IV	ACGT	4 cut(s)	174, 328, 406, 852
HpyCH4V	TGCA	8 cut(s)	77, 269, 563, 1428, 1439, 1595, 2096, 2114
HpyF10VI	GCNNNNNNNGC	5 cut(s)	332, 424, 830, 1073, 1536
HpyF3I	CTNAG	5 cut(s)	624, 687, 840, 1033, 2090
HpySE526I	ACGT	4 cut(s)	174, 328, 406, 852
HspAI	GCGC	3 cut(s)	571, 586, 831
Kpn2I	TCCGGA	2 cut(s)	361, 2021
KspAI	GTTAAC	1 cut(s)	106
KspI	CCGCGG	1 cut(s)	1954
Kzo9I	GATC	8 cut(s)	26, 614, 1276, 1372, 1627, 1640, 1656, 1738
LmnI	GCTCC	2 cut(s)	343, 679
Lsp1109I	GCAGC	3 cut(s)	322, 1603, 1913
LweI	GCATC	6 cut(s)	438, 493, 1111, 1308, 1928, 2123
MaeI	CTAG	4 cut(s)	785, 1041, 1877, 2004
MaeII	ACGT	4 cut(s)	174, 328, 406, 852
MaeIII	GTNAC	9 cut(s)	46, 308, 369, 853, 1036, 1421, 1777, 1813, 2124
MalI	GATC	8 cut(s)	28, 616, 1278, 1374, 1629, 1642, 1658, 1740
MboI	GATC	8 cut(s)	26, 614, 1276, 1372, 1627, 1640, 1656, 1738
MfeI	CAATTG	1 cut(s)	438
MflI	RGATCY	3 cut(s)	26, 614, 1656
MhlI	GDGCHC	4 cut(s)	849, 1294, 1477, 2098
MlsI	TGGCCA	1 cut(s)	116
MluCI	AATT	9 cut(s)	438, 443, 558, 609, 1173, 1365, 1489, 1512, 1607
MluNI	TGGCCA	1 cut(s)	116
MlyI	GAGTC	1 cut(s)	7
MmeI	TCCRAC	4 cut(s)	40, 1134, 1366, 1579
Mox20I	TGGCCA	1 cut(s)	116
MroI	TCCGGA	2 cut(s)	361, 2021
MroXI	GAANNNNTTC	2 cut(s)	211, 990
MscI	TGGCCA	1 cut(s)	116
MseI	TTAA	5 cut(s)	105, 752, 870, 1026, 1808
Msp20I	TGGCCA	1 cut(s)	116
MspA1I	CMGCKG	2 cut(s)	1953, 2089
MspI	CCGG	2 cut(s)	362, 2022
MspR9I	CCNGG	2 cut(s)	387, 1794
MunI	CAATTG	1 cut(s)	438
Mva1269I	GAATGC	1 cut(s)	1451
MvaI	CCWGG	2 cut(s)	387, 1794
MvnI	CGCG	2 cut(s)	831, 1953
MwoI	GCNNNNNNNGC	5 cut(s)	332, 424, 830, 1073, 1536
NcoI	CCATGG	1 cut(s)	30
NdeII	GATC	8 cut(s)	26, 614, 1276, 1372, 1627, 1640, 1656, 1738
NlaIV	GGNNCC	6 cut(s)	28, 320, 616, 846, 1474, 1551
NmuCI	GTSAC	3 cut(s)	46, 369, 2124
NspI	RCATGY	3 cut(s)	702, 1869, 2134
PaeI	GCATGC	2 cut(s)	702, 1869
PaeR7I	CTCGAG	1 cut(s)	1187
PctI	GAATGC	1 cut(s)	1451
PdmI	GAANNNNTTC	2 cut(s)	211, 990
PfeI	GAWTC	2 cut(s)	382, 1766
PflFI	GACNNNGTC	2 cut(s)	1130, 1520
PflMI	CCANNNNNTGG	1 cut(s)	1483
PfoI	TCCNGGA	1 cut(s)	385
PkrI	GCNGC	4 cut(s)	337, 1618, 1903, 2088
PleI	GAGTC	1 cut(s)	7
PpsI	GAGTC	1 cut(s)	7
Ppu21I	YACGTR	1 cut(s)	329
PpuMI	RGGWCCY	1 cut(s)	1550
Psp1406I	AACGTT	1 cut(s)	852
Psp5II	RGGWCCY	1 cut(s)	1550
Psp6I	CCWGG	2 cut(s)	385, 1792
PspEI	GGTNACC	1 cut(s)	46
PspFI	CCCAGC	2 cut(s)	158, 490
PspGI	CCWGG	2 cut(s)	385, 1792
PspN4I	GGNNCC	6 cut(s)	28, 320, 616, 846, 1474, 1551
PspPI	GGNCC	4 cut(s)	937, 1550, 1985, 2019
PspPPI	RGGWCCY	1 cut(s)	1550
PsuI	RGATCY	3 cut(s)	26, 614, 1656
PsyI	GACNNNGTC	2 cut(s)	1130, 1520
RsaI	GTAC	1 cut(s)	1338
RsaNI	GTAC	1 cut(s)	1337
SacII	CCGCGG	1 cut(s)	1954
SaqAI	TTAA	5 cut(s)	105, 752, 870, 1026, 1808
SatI	GCNGC	4 cut(s)	336, 1617, 1902, 2087
Sau3AI	GATC	8 cut(s)	26, 614, 1276, 1372, 1627, 1640, 1656, 1738
Sau96I	GGNCC	4 cut(s)	937, 1550, 1985, 2019
SchI	GAGTC	1 cut(s)	7
ScrFI	CCNGG	2 cut(s)	387, 1794
SduI	GDGCHC	4 cut(s)	849, 1294, 1477, 2098
SfaNI	GCATC	6 cut(s)	438, 493, 1111, 1308, 1928, 2123
SfcI	CTRYAG	1 cut(s)	1851
Sfr274I	CTCGAG	1 cut(s)	1187
Sfr303I	CCGCGG	1 cut(s)	1954
SgrBI	CCGCGG	1 cut(s)	1954
SinI	GGWCC	2 cut(s)	1550, 2019
SlaI	CTCGAG	1 cut(s)	1187
SmlI	CTYRAG	3 cut(s)	519, 1187, 2009
SmoI	CTYRAG	3 cut(s)	519, 1187, 2009
SphI	GCATGC	2 cut(s)	702, 1869
Sse9I	AATT	9 cut(s)	438, 443, 558, 609, 1173, 1365, 1489, 1512, 1607
SsiI	CCGC	5 cut(s)	788, 1124, 1951, 1953, 2087
SspMI	CTAG	4 cut(s)	785, 1041, 1877, 2004
StyD4I	CCNGG	2 cut(s)	385, 1792
StyI	CCWWGG	5 cut(s)	30, 51, 111, 413, 875
TaaI	ACNGT	5 cut(s)	579, 743, 1384, 1813, 1845
TaiI	ACGT	4 cut(s)	177, 331, 409, 855
TaqI	TCGA	8 cut(s)	150, 225, 240, 1010, 1188, 1447, 1771, 1897
TasI	AATT	9 cut(s)	438, 443, 558, 609, 1173, 1365, 1489, 1512, 1607
TauI	GCSGC	1 cut(s)	2089
TfiI	GAWTC	2 cut(s)	382, 1766
Tru1I	TTAA	5 cut(s)	105, 752, 870, 1026, 1808
Tru9I	TTAA	5 cut(s)	105, 752, 870, 1026, 1808
TscAI	CASTG	3 cut(s)	205, 748, 1214
TseFI	GTSAC	3 cut(s)	46, 369, 2124
TseI	GCWGC	3 cut(s)	335, 1616, 1901
Tsp45I	GTSAC	3 cut(s)	46, 369, 2124
TspDTI	ATGAA	2 cut(s)	287, 719
TspGWI	ACGGA	2 cut(s)	770, 1354
TspRI	CASTG	3 cut(s)	205, 748, 1214
Tth111I	GACNNNGTC	2 cut(s)	1130, 1520
Van91I	CCANNNNNTGG	1 cut(s)	1483
VneI	GTGCAC	1 cut(s)	2094
VpaK11BI	GGWCC	2 cut(s)	1550, 2019
XagI	CCTNNNNNAGG	1 cut(s)	623
XapI	RAATTY	2 cut(s)	1173, 1607
XbaI	TCTAGA	1 cut(s)	1876
XceI	RCATGY	3 cut(s)	702, 1869, 2134
XhoI	CTCGAG	1 cut(s)	1187
XmnI	GAANNNNTTC	2 cut(s)	211, 990
XspI	CTAG	4 cut(s)	785, 1041, 1877, 2004

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.