pycom15g17420

Lachrymatory-factor synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006645
Physical Location & Seq
Reverse (-)
12286195 .. 12286752
558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGGCACATGAGAAACAACTGAAATGGGAAGGCGAGGCAAGTGCAGAGCTAAAAGGCCCCAAAGCAGAGCAAGTTTGGCTTCTTTTGGAGGACTTCCTTGGCCTCCACAAGTGGTTCCCAACTCTCACAACTTGCCTACTTGCCTTGGTGTTGAAGGCACGTCGGGACCAAGAGAAGGTGAATTGGACTAAGCAGAAGCCGCTGGGTAAAGACCCAGCAACAATGACATTTAGTTACTCTATCATATATGGGAATGTCGGGTTCAACTCGTACATTTCGACCGTGGAAGTGGTGCGGAAGGATGCTGGGTGCACGATTGTGTGGAAGTATGAAGTTGAACGAGTGGAGGGATGGAGGCTGGAGGATCTGGACCTGTTTATTGGCACTGGCTTGCAAGTCATGGCTAGCAGAATGGAAGCCTCTCTGTATAGCCCTGAAATAAAACCCGGTGATAAATCCGGAAAGGTTGATAAATTGTCGGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

163

Amino Acids

18.4

Weight (kDa)

6.16

Isoelectric Point (pI)

24.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 14 - 119 3.2e-12 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 458
AciI CCGC 2 cut(s) 200, 295
AclWI GGATC 1 cut(s) 372
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 175
AgsI TTSAA 3 cut(s) 154, 265, 338
AjiI CACGTC 1 cut(s) 161
AleI CACNNNNGTG 1 cut(s) 317
AluBI AGCT 1 cut(s) 49
AluI AGCT 1 cut(s) 49
Alw21I GWGCWC 1 cut(s) 314
Alw44I GTGCAC 1 cut(s) 310
AlwI GGATC 1 cut(s) 372
Aor13HI TCCGGA 1 cut(s) 458
AoxI GGCC 2 cut(s) 55, 100
ApaLI GTGCAC 1 cut(s) 310
AspS9I GGNCC 3 cut(s) 56, 166, 370
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 2 cut(s) 190, 461
AsuNHI GCTAGC 1 cut(s) 404
AvaII GGWCC 2 cut(s) 166, 370
BaeGI GKGCMC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 314
BccI CCATC 1 cut(s) 345
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BcnI CCSGG 1 cut(s) 447
BfaI CTAG 1 cut(s) 405
BisI GCNGC 1 cut(s) 200
BlsI GCNGC 1 cut(s) 201
Bme1390I CCNGG 1 cut(s) 447
Bme18I GGWCC 2 cut(s) 166, 370
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 3 cut(s) 56, 166, 370
BmiI GGNNCC 3 cut(s) 58, 116, 167
BmrFI CCNGG 1 cut(s) 447
BmsI GCATC 1 cut(s) 292
BmtI GCTAGC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 97, 144, 282
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 391
BseAI TCCGGA 1 cut(s) 458
BseDI CCNNGG 3 cut(s) 97, 144, 282
BseGI GGATG 2 cut(s) 307, 356
BseLI CCNNNNNNNGG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 391
BseSI GKGCMC 1 cut(s) 314
BseYI CCCAGC 3 cut(s) 202, 214, 305
BsgI GTGCAG 1 cut(s) 63
Bsh1285I CGRYCG 1 cut(s) 282
BshFI GGCC 2 cut(s) 57, 102
BsiEI CGRYCG 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 314
BsiSI CCGG 2 cut(s) 447, 459
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 175
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 2 cut(s) 57, 102
Bsp1286I GDGCHC 1 cut(s) 314
Bsp13I TCCGGA 1 cut(s) 458
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 2 cut(s) 200, 295
BspANI GGCC 2 cut(s) 57, 102
BspEI TCCGGA 1 cut(s) 458
BspLI GGNNCC 3 cut(s) 58, 116, 167
BspOI GCTAGC 1 cut(s) 408
BspPI GGATC 1 cut(s) 372
BsrI ACTGG 1 cut(s) 391
BssECI CCNNGG 3 cut(s) 97, 144, 282
BssMI GATC 1 cut(s) 364
BssT1I CCWWGG 2 cut(s) 97, 144
Bst4CI ACNGT 1 cut(s) 283
BstC8I GCNNGC 2 cut(s) 392, 406
BstDEI CTNAG 1 cut(s) 189
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 2 cut(s) 307, 356
BstKTI GATC 1 cut(s) 367
BstMBI GATC 1 cut(s) 364
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 2 cut(s) 76, 199
BstSCI CCNGG 1 cut(s) 445
BstSLI GKGCMC 1 cut(s) 314
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuRI GGCC 2 cut(s) 57, 102
BtgI CCRYGG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 2 cut(s) 307, 356
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 392, 406
Cfr13I GGNCC 3 cut(s) 56, 166, 370
Csp6I GTAC 1 cut(s) 271
CviAII CATG 2 cut(s) 8, 400
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 1 cut(s) 189
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 97, 144
Eco47I GGWCC 2 cut(s) 166, 370
EcoO109I RGGNCCY 1 cut(s) 56
EcoT14I CCWWGG 2 cut(s) 97, 144
ErhI CCWWGG 2 cut(s) 97, 144
FaeI CATG 2 cut(s) 11, 403
FaiI YATR 7 cut(s) 9, 245, 247, 249, 330, 401, 429
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FaqI GGGAC 1 cut(s) 179
FatI CATG 2 cut(s) 7, 399
Fnu4HI GCNGC 1 cut(s) 200
FokI GGATG 2 cut(s) 314, 363
Fsp4HI GCNGC 1 cut(s) 200
FspBI CTAG 1 cut(s) 405
GluI GCNGC 1 cut(s) 200
GsaI CCCAGC 3 cut(s) 206, 218, 309
GsuI CTGGAG 1 cut(s) 380
HaeIII GGCC 2 cut(s) 57, 102
HapII CCGG 2 cut(s) 447, 459
Hin1II CATG 2 cut(s) 11, 403
HpaII CCGG 2 cut(s) 447, 459
HphI GGTGA 2 cut(s) 190, 461
Hpy166II GTNNAC 1 cut(s) 312
Hpy188I TCNGA 1 cut(s) 481
Hpy188III TCNNGA 3 cut(s) 164, 368, 459
Hpy8I GTNNAC 1 cut(s) 312
Hpy99I CGWCG 1 cut(s) 165
HpyAV CCTTC 4 cut(s) 23, 148, 169, 292
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 44, 312, 394
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 199
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 2 cut(s) 11, 403
Kpn2I TCCGGA 1 cut(s) 458
Kzo9I GATC 1 cut(s) 364
LweI GCATC 1 cut(s) 292
MaeI CTAG 1 cut(s) 405
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 1 cut(s) 233
MalI GATC 1 cut(s) 366
MboI GATC 1 cut(s) 364
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 2 cut(s) 181, 473
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 7 cut(s) 28, 82, 113, 340, 348, 355, 430
MroI TCCGGA 1 cut(s) 458
MslI CAYNNNNRTG 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 2 cut(s) 447, 459
MspR9I CCNGG 1 cut(s) 447
MwoI GCNNNNNNNGC 2 cut(s) 76, 199
NciI CCSGG 1 cut(s) 447
NdeII GATC 1 cut(s) 364
NheI GCTAGC 1 cut(s) 404
NlaIII CATG 2 cut(s) 11, 403
NlaIV GGNNCC 3 cut(s) 58, 116, 167
OliI CACNNNNGTG 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PkrI GCNGC 1 cut(s) 201
PspFI CCCAGC 3 cut(s) 202, 214, 305
PspN4I GGNNCC 3 cut(s) 58, 116, 167
PspPI GGNCC 3 cut(s) 56, 166, 370
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 317
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 1 cut(s) 364
Sau96I GGNCC 3 cut(s) 56, 166, 370
ScrFI CCNGG 1 cut(s) 447
SduI GDGCHC 1 cut(s) 314
SetI ASST 5 cut(s) 51, 163, 180, 375, 468
SfaNI GCATC 1 cut(s) 292
SinI GGWCC 2 cut(s) 166, 370
SmiMI CAYNNNNRTG 1 cut(s) 317
Sse9I AATT 2 cut(s) 181, 473
SsiI CCGC 2 cut(s) 200, 295
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 445
StyI CCWWGG 2 cut(s) 97, 144
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 278
TasI AATT 2 cut(s) 181, 473
TauI GCSGC 1 cut(s) 202
TscAI CASTG 1 cut(s) 391
TspDTI ATGAA 1 cut(s) 345
TspRI CASTG 1 cut(s) 391
VneI GTGCAC 1 cut(s) 310
VpaK11BI GGWCC 2 cut(s) 166, 370
XspI CTAG 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.