Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0003680

Physical Location & Seq

Genomic Coordinates Forward (+)

13518104 .. 13521493

Gene Structure 3390 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGCAACACTGTGCTGCAAGTTTGCTTCGAGCTGTGTCTCTCTCCGGACAACATTGCAAAGATTCCCGAGAAAGTTCAAAACTTTCCCGGAAAATTCAAAGGGTATTTTCATATTTTTCTCACTGCCCAAATAAGTATCAAAGCTTGAATCAGTGGCGGAGAAAAGTGACAATGGAAGGTGGAAAAGGCGTGGGGGAGAAGAACGGAACAAACGATGTTGTTTTGCAGATTTTGGCCGGCGAAGAGCCCACAAGTTACAACAATGAAACCAGAGACTTGAATCTTCACCTGACGGAGCTTCAGAGCCTCAGAGTCTCCGGCTCTTCTTCGCCAGAAATCCCAAGGCTGAGTCGTACGCCGCCGCCGGGGAAGCCGCCGAAAATCCCAACCGAACCCAGTTCCCTGCAAGCTCCGTTTTCGCGGTCGGTCTTCTCGAAGCCCAAGTCGAGGTTCATGGAGCCGTTTCCCCAGTTGAAGTCAATTGCTAATTCCTCCAATGTGGAATCGCCAAGCTCCAAAATCGCAGGCACAAAACCGAGAGACGCTCTGAGGTCCGCCTCGATAACCCCACGAACACCGTTGGTCGACGGTGGCGAGGAGGACGATGATGAGGAGGTTTATAAGACTGCAAAGCTAAAGGTGTGTGCGAAGTCCGGTAAGAAGATAAAAAAGTTGGCATTGATTGAGTTGGTTGCATTTGTTTGCATTGTTTTGTTTTTGATTGCTGGCATAACTGTACCCACGTTGGAAAATAAGATGTATTTGGGGTTGGAATTGTGGAAATGGTGTGTCTTGGTGTTGGTTGTTTTGTGTGGTAGGTTGGTCACAGAATGGTTTATCAATGTTCTGGTTTTCGTGATTGAGTTGAACTTTTTGCTTAAGAAGAATGTTTTGTATTTTGTGTACGGGTTGAAGAGAAGTGTACAGATTTTTATTTGGTTGGGTTTGGTTCTTCTGGCTTGGGGTTTGTTGTTCGACAACGGAGGGAAGAGGTCAAGCAAAACCTCTAGGATCCTAGGTTATGTTACACGGGGTCTAGCTTCATGCCTAATTGGAGCGGCCATATGGCTGGTGAAGAATTTGTTTGTCAAAGTAGTAGCTTCTTCATTCCAATGCAATAGATTCTTTGATCGGATTCAAGAATCAATCTTTCATCAGTATGTTCTCCGCACCCTTTCCGGGCCTCCACTGATGGAAATGGCAGAAAGAATCGGGAGGGCCCCAAGTAGCGGTCGGTTGAGTTTCAAAATTTTGAAGGGAGGGAAAAAGGAGGGGAATGAAGGGGCCAAAGAGGAGGTGATTGATGTAGAAAAGCTCAAAAAGATGAACCAAGAGAAAATTTCTGCTTGGACCTTGAAAGGGTTGATTAATGTCGTAAGGAGTACGGGGTTATCTACCATCTCCGACTCACTTGAGAGTCTTGACGACGAGGAGAGTGAGCAGAAAGGTAAGGAGATTACTAGTGAGTGGGAAGCAAGGGCTGCGGCTTATGATATTTTCCTGAATGTCGCCAAGAGTAACAAGTACATTGAGGAAGATGACCTCTTGCGCTTCATGAAAAAGGAGGAGGTCGACATTGTTTTACCTCTGTTTGAAGGGGCAGCAGAAACTGGCAAGATCAAGAGAAAAGCTTTGAAGAACTGGCTGGTGAATGTTTACCTTGAGCGCAAATCACTGGCGCATTCCTTAAATGACACTAAAACAGCAATAGAGGAGCTGAATAAGCTTGCCTCAGGGTTTTTGCTTCTGGTGATCATTATCGTGTGGCTACTTGTGATGGGATTTTTCACGACAAACATGCTGGTCTTTATAGCATCTCAGCTTTTAACGCTGGCGTTTGTATTTGGTAATACTGCTAGAACTGTGTTTGAAGCGATCATATTCGTCTTTGTGATGCACCCTTTTGATGTAGGGGACCGTTGTGTCGTTGATGGAGTACAGCTTATTGTTGAAGAGATGAACATATTGACAACGGTCTTCTTAAGATCTGACAACGAGAAAGTTTTCTATCCAAATTCAGTACTGGCTAGTAAACCCATCAGCAACTTCTATAGGAGCCCGGAGATGGGTGATTCTGTTGATTTTACCATTGACGTTTCTACGAAGGTTGAGACCATTGTGGCTCTAAAAGCCAGAATAAAAGAGTACTTGGACAGCAAGACTCAGCACTGGCGTCCTGCGCATAGCGTGGTGGTTAAGGATATTGAGGACGTTAACAAAATGAAAATGGCTCTTTACGTTACACATACCATAAACTTTCAGAACTATGGCGACAAGACCAGTCGGAGATCAGATTTGATCTTCGAGCTGAAGAAAATATTTGAAGATCTTGGTATAAAATATCATCTCCTGCCTCAAGAAGTTCATCTCCGCTATGTCGGATCATCCGCTGCAGAACTTCCACCCACATGGCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel PsbX

Protein Analysis

806

Amino Acids

90.69

Weight (kDa)

9.2

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	231 - 370	7.4e-07	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	627 - 683	2.4e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003680)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g08260 FvH4_1g08260 FvH4_1g08260 FvH4_1g08260
malus_domestica	MD02G1086900.v1.1 MD02G1087000.v1.1 MD15G1214500.v1.1 MD15G1214600.v1.1
prunus_persica	Prupe.7G202700_v2.0.a1
pyrus_communis	pycom02g06990 pycom02g07000 pycom15g19040
rosa_chinensis	RchiOBHm_Chr2g0094331
rosa_laevigata	RLG00000016480
rosa_multiflora	Rmu_sc0003819.1_g000016 Rmu_sc0025410.1_g000001 Rmu_sc0025411.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147230 Rroxscaffold_7G00185070
rosa_rugosa	Rorug02G0043300 Rorug02G0043300
rosa_samantha	Rh2AG091000 Rh2AG091100 Rh2BG090900 Rh2CG093400 Rh2CG093500 Rh2DG089900 Rh6AG505100 Rh6BG272500 Rh6CG273600 Rh6CG521500 Rh6CG521800 Rh6DG266600 Rh6DG507600
rosa_wichuraiana	Rw6G043950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	621
AasI	GACNNNNNNGTC	1 cut(s)	1922
Acc16I	TGCGCA	1 cut(s)	2181
AccBSI	CCGCTC	1 cut(s)	1058
AccI	GTMKAC	2 cut(s)	585, 1572
AccII	CGCG	1 cut(s)	421
AccIII	TCCGGA	1 cut(s)	44
AclWI	GGATC	3 cut(s)	1006, 1019, 2389
AcoI	YGGCCR	2 cut(s)	234, 1059
AcsI	RAATTY	5 cut(s)	93, 1078, 1248, 1338, 2014
AcuI	CTGAAG	2 cut(s)	284, 2330
AcyI	GRCGYC	1 cut(s)	2173
AfaI	GTAC	9 cut(s)	355, 738, 905, 924, 1384, 1526, 1938, 2022, 2147
AfiI	CCNNNNNNNGG	8 cut(s)	365, 447, 1179, 1229, 1359, 2065, 2066, 2412
AflII	CTTAAG	2 cut(s)	878, 1981
AhlI	ACTAGT	1 cut(s)	1460
AjuI	GAANNNNNNNTTGG	2 cut(s)	434, 466
Alw26I	GTCTC	5 cut(s)	42, 267, 319, 534, 2105
AlwI	GGATC	3 cut(s)	1006, 1019, 2389
AlwNI	CAGNNNCTG	1 cut(s)	1610
Ama87I	CYCGRG	1 cut(s)	66
Aor13HI	TCCGGA	1 cut(s)	44
AoxI	GGCC	5 cut(s)	234, 1059, 1181, 1218, 1284
ApaI	GGGCCC	1 cut(s)	1222
ApeKI	GCWGC	4 cut(s)	14, 1481, 1601, 2390
ApoI	RAATTY	5 cut(s)	93, 1078, 1248, 1338, 2014
ArsI	GACNNNNNNTTYG	2 cut(s)	428, 460
AseI	ATTAAT	1 cut(s)	1368
Asp700I	GAANNNNTTC	1 cut(s)	2003
AspA2I	CCTAGG	1 cut(s)	1015
AspLEI	GCGC	4 cut(s)	1551, 1668, 1681, 2182
AspS9I	GGNCC	7 cut(s)	552, 1181, 1218, 1219, 1284, 1350, 1915
AsuC2I	CCSGG	4 cut(s)	88, 366, 1180, 2060
AsuHPI	GGTGA	6 cut(s)	278, 1084, 1309, 1660, 1762, 2081
AvaI	CYCGRG	1 cut(s)	66
AvaII	GGWCC	3 cut(s)	552, 1350, 1915
AvrII	CCTAGG	1 cut(s)	1015
AxyI	CCTNAGG	1 cut(s)	1732
BaeGI	GKGCMC	1 cut(s)	1222
BamHI	GGATCC	1 cut(s)	1011
BanII	GRGCYC	3 cut(s)	249, 1222, 2060
BbsI	GAAGAC	2 cut(s)	421, 1969
BbvI	GCAGC	3 cut(s)	1468, 1613, 2377
BccI	CCATC	6 cut(s)	1186, 1406, 1771, 1925, 2045, 2059
BceAI	ACGGC	1 cut(s)	445
BcgI	CGANNNNNNTGC	2 cut(s)	1780, 1814
BclI	TGATCA	1 cut(s)	1752
BcnI	CCSGG	4 cut(s)	88, 366, 1180, 2060
BcoDI	GTCTC	5 cut(s)	42, 267, 319, 534, 2105
BcuI	ACTAGT	1 cut(s)	1460
BfaI	CTAG	6 cut(s)	1008, 1016, 1037, 1461, 1857, 2028
BfmI	CTRYAG	2 cut(s)	2050, 2391
BfrI	CTTAAG	2 cut(s)	878, 1981
BglII	AGATCT	2 cut(s)	1985, 2326
BisI	GCNGC	9 cut(s)	15, 359, 362, 374, 1059, 1482, 1485, 1602, 2391
BlnI	CCTAGG	1 cut(s)	1015
BlsI	GCNGC	9 cut(s)	16, 360, 363, 375, 1060, 1483, 1486, 1603, 2392
BmcAI	AGTACT	2 cut(s)	2022, 2147
Bme1390I	CCNGG	4 cut(s)	88, 366, 1180, 2060
Bme18I	GGWCC	3 cut(s)	552, 1350, 1915
BmeT110I	CYCGRG	1 cut(s)	66
BmgT120I	GGNCC	7 cut(s)	552, 1181, 1218, 1219, 1284, 1350, 1915
BmiI	GGNNCC	7 cut(s)	459, 1013, 1220, 1221, 1285, 1916, 2057
BmrFI	CCNGG	4 cut(s)	88, 366, 1180, 2060
BmrI	ACTGGG	2 cut(s)	390, 463
BmsI	GCATC	2 cut(s)	1823, 1884
BmuI	ACTGGG	2 cut(s)	390, 463
BoxI	GACNNNNGTC	1 cut(s)	1973
BpiI	GAAGAC	2 cut(s)	421, 1969
BplI	GAGNNNNNCTC	2 cut(s)	529, 561
BpuEI	CTTGAG	3 cut(s)	1433, 1682, 2340
BpuMI	CCSGG	4 cut(s)	88, 366, 1180, 2060
BsaBI	GATNNNNATC	1 cut(s)	1757
BsaHI	GRCGYC	1 cut(s)	2173
BsaI	GGTCTC	1 cut(s)	2105
BsaJI	CCNNGG	3 cut(s)	341, 365, 1015
BsaWI	WCCGGW	2 cut(s)	44, 653
Bsc4I	CCNNNNNNNGG	8 cut(s)	365, 447, 1179, 1229, 1359, 2065, 2066, 2412
Bse118I	RCCGGY	1 cut(s)	236
Bse1I	ACTGG	8 cut(s)	396, 469, 1615, 1646, 1680, 2028, 2174, 2280
Bse21I	CCTNAGG	1 cut(s)	1732
Bse3DI	GCAATG	1 cut(s)	52
Bse8I	GATNNNNATC	1 cut(s)	1757
BseAI	TCCGGA	1 cut(s)	44
BseDI	CCNNGG	3 cut(s)	341, 365, 1015
BseGI	GGATG	1 cut(s)	2384
BseJI	GATNNNNATC	1 cut(s)	1757
BseLI	CCNNNNNNNGG	8 cut(s)	365, 447, 1179, 1229, 1359, 2065, 2066, 2412
BseMI	GCAATG	1 cut(s)	52
BseMII	CTCAG	6 cut(s)	322, 338, 539, 1746, 1832, 2177
BseNI	ACTGG	8 cut(s)	396, 469, 1615, 1646, 1680, 2028, 2174, 2280
BseRI	GAGGAG	6 cut(s)	611, 626, 1307, 1445, 1580, 1727
BseSI	GKGCMC	1 cut(s)	1222
BseXI	GCAGC	3 cut(s)	1468, 1613, 2377
Bsh1236I	CGCG	1 cut(s)	421
Bsh1285I	CGRYCG	2 cut(s)	425, 1234
BshFI	GGCC	5 cut(s)	236, 1061, 1183, 1220, 1286
BsiEI	CGRYCG	2 cut(s)	425, 1234
BsiHKCI	CYCGRG	1 cut(s)	66
BsiSI	CCGG	8 cut(s)	45, 88, 237, 318, 365, 654, 1179, 2060
BsiWI	CGTACG	1 cut(s)	353
BslFI	GGGAC	1 cut(s)	1928
BslI	CCNNNNNNNGG	8 cut(s)	365, 447, 1179, 1229, 1359, 2065, 2066, 2412
BsmAI	GTCTC	5 cut(s)	42, 267, 319, 534, 2105
BsmBI	CGTCTC	1 cut(s)	534
BsmFI	GGGAC	1 cut(s)	1928
BsmI	GAATGC	1 cut(s)	1681
BsnI	GGCC	5 cut(s)	236, 1061, 1183, 1220, 1286
Bso31I	GGTCTC	1 cut(s)	2105
BsoBI	CYCGRG	1 cut(s)	66
Bsp120I	GGGCCC	1 cut(s)	1218
Bsp1286I	GDGCHC	3 cut(s)	249, 1222, 2060
Bsp13I	TCCGGA	1 cut(s)	44
Bsp1407I	TGTACA	1 cut(s)	922
BspANI	GGCC	5 cut(s)	236, 1061, 1183, 1220, 1286
BspCNI	CTCAG	6 cut(s)	321, 339, 540, 1745, 1831, 2176
BspEI	TCCGGA	1 cut(s)	44
BspFNI	CGCG	1 cut(s)	421
BspHI	TCATGA	1 cut(s)	1554
BspLI	GGNNCC	7 cut(s)	459, 1013, 1220, 1221, 1285, 1916, 2057
BspMAI	CTGCAG	1 cut(s)	2395
BspPI	GGATC	3 cut(s)	1006, 1019, 2389
BspQI	GCTCTTC	2 cut(s)	237, 328
BspTI	CTTAAG	2 cut(s)	878, 1981
BspTNI	GGTCTC	1 cut(s)	2105
BsrBI	CCGCTC	1 cut(s)	1058
BsrDI	GCAATG	1 cut(s)	52
BsrFI	RCCGGY	1 cut(s)	236
BsrGI	TGTACA	1 cut(s)	922
BsrI	ACTGG	8 cut(s)	396, 469, 1615, 1646, 1680, 2028, 2174, 2280
BssAI	RCCGGY	1 cut(s)	236
BssECI	CCNNGG	3 cut(s)	341, 365, 1015
BssNI	GRCGYC	1 cut(s)	2173
BssT1I	CCWWGG	2 cut(s)	341, 1015
Bst4CI	ACNGT	7 cut(s)	11, 579, 590, 736, 1864, 1919, 1975
Bst6I	CTCTTC	5 cut(s)	237, 328, 908, 983, 1947
BstACI	GRCGYC	1 cut(s)	2173
BstAFI	CTTAAG	2 cut(s)	878, 1981
BstAPI	GCANNNNNTGC	1 cut(s)	1481
BstAUI	TGTACA	1 cut(s)	922
BstC8I	GCNNGC	6 cut(s)	238, 408, 526, 727, 1728, 1833
BstDEI	CTNAG	6 cut(s)	308, 347, 548, 1732, 1818, 2163
BstF5I	GGATG	1 cut(s)	2384
BstFNI	CGCG	1 cut(s)	421
BstHHI	GCGC	4 cut(s)	1551, 1668, 1681, 2182
BstMAI	GTCTC	5 cut(s)	42, 267, 319, 534, 2105
BstMCI	CGRYCG	2 cut(s)	425, 1234
BstMWI	GCNNNNNNNGC	6 cut(s)	370, 1481, 1723, 1828, 2129, 2179
BstNSI	RCATGY	1 cut(s)	1801
BstPAI	GACNNNNGTC	1 cut(s)	1973
BstSCI	CCNGG	4 cut(s)	86, 364, 1178, 2058
BstSFI	CTRYAG	2 cut(s)	2050, 2391
BstSLI	GKGCMC	1 cut(s)	1222
BstUI	CGCG	1 cut(s)	421
BstV1I	GCAGC	3 cut(s)	1468, 1613, 2377
BstV2I	GAAGAC	2 cut(s)	421, 1969
BstX2I	RGATCY	3 cut(s)	1011, 1985, 2326
BstXI	CCANNNNNNTGG	2 cut(s)	1069, 2409
BstYI	RGATCY	3 cut(s)	1011, 1985, 2326
Bsu36I	CCTNAGG	1 cut(s)	1732
BsuRI	GGCC	5 cut(s)	236, 1061, 1183, 1220, 1286
BtsCI	GGATG	1 cut(s)	2384
BtsI	GCAGTG	1 cut(s)	121
BtsIMutI	CAGTG	6 cut(s)	7, 121, 158, 1187, 1673, 2167
Cac8I	GCNNGC	6 cut(s)	238, 408, 526, 727, 1728, 1833
CaiI	CAGNNNCTG	1 cut(s)	1610
CciI	TCATGA	1 cut(s)	1554
CfoI	GCGC	4 cut(s)	1551, 1668, 1681, 2182
Cfr10I	RCCGGY	1 cut(s)	236
Cfr13I	GGNCC	7 cut(s)	552, 1181, 1218, 1219, 1284, 1350, 1915
CseI	GACGC	2 cut(s)	551, 2162
Csp6I	GTAC	9 cut(s)	354, 737, 904, 923, 1383, 1525, 1937, 2021, 2146
CviAII	CATG	5 cut(s)	454, 1044, 1555, 1798, 2409
CviQI	GTAC	9 cut(s)	354, 737, 904, 923, 1383, 1525, 1937, 2021, 2146
DdeI	CTNAG	6 cut(s)	308, 347, 548, 1732, 1818, 2163
DrdI	GACNNNNNNGTC	1 cut(s)	1922
DseDI	GACNNNNNNGTC	1 cut(s)	1922
EaeI	YGGCCR	2 cut(s)	234, 1059
Eam1104I	CTCTTC	5 cut(s)	237, 328, 908, 983, 1947
EarI	CTCTTC	5 cut(s)	237, 328, 908, 983, 1947
EciI	GGCGGA	2 cut(s)	172, 544
Eco130I	CCWWGG	2 cut(s)	341, 1015
Eco24I	GRGCYC	3 cut(s)	249, 1222, 2060
Eco31I	GGTCTC	1 cut(s)	2105
Eco47I	GGWCC	3 cut(s)	552, 1350, 1915
Eco57I	CTGAAG	2 cut(s)	284, 2330
Eco81I	CCTNAGG	1 cut(s)	1732
Eco88I	CYCGRG	1 cut(s)	66
EcoO109I	RGGNCCY	2 cut(s)	1218, 1219
EcoT14I	CCWWGG	2 cut(s)	341, 1015
EcoT38I	GRGCYC	3 cut(s)	249, 1222, 2060
ErhI	CCWWGG	2 cut(s)	341, 1015
Esp3I	CGTCTC	1 cut(s)	534
FaeI	CATG	5 cut(s)	457, 1047, 1558, 1801, 2412
FaqI	GGGAC	1 cut(s)	1928
FatI	CATG	5 cut(s)	453, 1043, 1554, 1797, 2408
FauNDI	CATATG	1 cut(s)	1064
FbaI	TGATCA	1 cut(s)	1752
FblI	GTMKAC	2 cut(s)	585, 1572
Fnu4HI	GCNGC	9 cut(s)	15, 359, 362, 374, 1059, 1482, 1485, 1602, 2391
FokI	GGATG	1 cut(s)	2371
FriOI	GRGCYC	3 cut(s)	249, 1222, 2060
Fsp4HI	GCNGC	9 cut(s)	15, 359, 362, 374, 1059, 1482, 1485, 1602, 2391
FspBI	CTAG	6 cut(s)	1008, 1016, 1037, 1461, 1857, 2028
FspI	TGCGCA	1 cut(s)	2181
GlaI	GCGC	4 cut(s)	1550, 1667, 1680, 2181
GluI	GCNGC	9 cut(s)	15, 359, 362, 374, 1059, 1482, 1485, 1602, 2391
HaeIII	GGCC	5 cut(s)	236, 1061, 1183, 1220, 1286
HapII	CCGG	8 cut(s)	45, 88, 237, 318, 365, 654, 1179, 2060
HgaI	GACGC	2 cut(s)	551, 2162
HhaI	GCGC	4 cut(s)	1551, 1668, 1681, 2182
Hin1I	GRCGYC	1 cut(s)	2173
Hin1II	CATG	5 cut(s)	457, 1047, 1558, 1801, 2412
Hin6I	GCGC	4 cut(s)	1549, 1666, 1679, 2180
HinP1I	GCGC	4 cut(s)	1549, 1666, 1679, 2180
HincII	GTYRAC	3 cut(s)	586, 1573, 2215
HindII	GTYRAC	3 cut(s)	586, 1573, 2215
HindIII	AAGCTT	3 cut(s)	142, 1629, 1724
HpaI	GTTAAC	1 cut(s)	2215
HpaII	CCGG	8 cut(s)	45, 88, 237, 318, 365, 654, 1179, 2060
HphI	GGTGA	6 cut(s)	278, 1084, 1309, 1660, 1762, 2081
Hpy166II	GTNNAC	7 cut(s)	586, 904, 923, 1573, 1657, 2033, 2215
Hpy8I	GTNNAC	7 cut(s)	586, 904, 923, 1573, 1657, 2033, 2215
Hpy99I	CGWCG	2 cut(s)	590, 1430
HpyAV	CCTTC	5 cut(s)	170, 1249, 1274, 1589, 2098
HpyCH4III	ACNGT	7 cut(s)	11, 579, 590, 736, 1864, 1919, 1975
HpyCH4IV	ACGT	4 cut(s)	743, 2094, 2211, 2238
HpyF10VI	GCNNNNNNNGC	6 cut(s)	370, 1481, 1723, 1828, 2129, 2179
HpyF3I	CTNAG	6 cut(s)	308, 347, 548, 1732, 1818, 2163
HpySE526I	ACGT	4 cut(s)	743, 2094, 2211, 2238
Hsp92I	GRCGYC	1 cut(s)	2173
Hsp92II	CATG	5 cut(s)	457, 1047, 1558, 1801, 2412
HspAI	GCGC	4 cut(s)	1549, 1666, 1679, 2180
Kpn2I	TCCGGA	1 cut(s)	44
KroI	GCCGGC	1 cut(s)	236
KroNI	GCCGGC	1 cut(s)	238
Ksp22I	TGATCA	1 cut(s)	1752
KspAI	GTTAAC	1 cut(s)	2215
LguI	GCTCTTC	2 cut(s)	237, 328
LmnI	GCTCC	7 cut(s)	295, 415, 457, 518, 1055, 1714, 2055
Lsp1109I	GCAGC	3 cut(s)	1468, 1613, 2377
LweI	GCATC	2 cut(s)	1823, 1884
MaeI	CTAG	6 cut(s)	1008, 1016, 1037, 1461, 1857, 2028
MaeII	ACGT	4 cut(s)	743, 2094, 2211, 2238
MaeIII	GTNAC	6 cut(s)	166, 254, 823, 1024, 1517, 2239
MbiI	CCGCTC	1 cut(s)	1058
MfeI	CAATTG	1 cut(s)	480
MflI	RGATCY	3 cut(s)	1011, 1985, 2326
MhlI	GDGCHC	3 cut(s)	249, 1222, 2060
MluCI	AATT	9 cut(s)	93, 480, 487, 773, 1050, 1078, 1248, 1338, 2014
MlyI	GAGTC	5 cut(s)	321, 358, 1400, 1426, 2155
MmeI	TCCRAC	5 cut(s)	726, 750, 1428, 2264, 2359
MroI	TCCGGA	1 cut(s)	44
MroNI	GCCGGC	1 cut(s)	236
MroXI	GAANNNNTTC	1 cut(s)	2003
MseI	TTAA	7 cut(s)	879, 1368, 1688, 1826, 1982, 2196, 2214
MslI	CAYNNNNRTG	5 cut(s)	1111, 1158, 1760, 2407, 2413
MspA1I	CMGCKG	1 cut(s)	2390
MspCI	CTTAAG	2 cut(s)	878, 1981
MspI	CCGG	8 cut(s)	45, 88, 237, 318, 365, 654, 1179, 2060
MspR9I	CCNGG	4 cut(s)	88, 366, 1180, 2060
MunI	CAATTG	1 cut(s)	480
Mva1269I	GAATGC	1 cut(s)	1681
MvnI	CGCG	1 cut(s)	421
MwoI	GCNNNNNNNGC	6 cut(s)	370, 1481, 1723, 1828, 2129, 2179
NaeI	GCCGGC	1 cut(s)	238
NciI	CCSGG	4 cut(s)	88, 366, 1180, 2060
NdeI	CATATG	1 cut(s)	1064
NgoMIV	GCCGGC	1 cut(s)	236
NlaIII	CATG	5 cut(s)	457, 1047, 1558, 1801, 2412
NlaIV	GGNNCC	7 cut(s)	459, 1013, 1220, 1221, 1285, 1916, 2057
NmuCI	GTSAC	2 cut(s)	166, 823
NsbI	TGCGCA	1 cut(s)	2181
NspI	RCATGY	1 cut(s)	1801
PagI	TCATGA	1 cut(s)	1554
PciSI	GCTCTTC	2 cut(s)	237, 328
PcsI	WCGNNNNNNNCGW	3 cut(s)	210, 431, 591
PctI	GAATGC	1 cut(s)	1681
PdiI	GCCGGC	1 cut(s)	238
PdmI	GAANNNNTTC	1 cut(s)	2003
PfeI	GAWTC	9 cut(s)	62, 148, 280, 503, 1122, 1135, 1142, 1209, 2072
Pfl23II	CGTACG	1 cut(s)	353
PfoI	TCCNGGA	1 cut(s)	86
PkrI	GCNGC	9 cut(s)	16, 360, 363, 375, 1060, 1483, 1486, 1603, 2392
PleI	GAGTC	5 cut(s)	320, 357, 1400, 1425, 2155
PpsI	GAGTC	5 cut(s)	320, 357, 1400, 1425, 2155
PshAI	GACNNNNGTC	1 cut(s)	1973
PshBI	ATTAAT	1 cut(s)	1368
PsiI	TTATAA	1 cut(s)	621
PspLI	CGTACG	1 cut(s)	353
PspN4I	GGNNCC	7 cut(s)	459, 1013, 1220, 1221, 1285, 1916, 2057
PspOMI	GGGCCC	1 cut(s)	1218
PspPI	GGNCC	7 cut(s)	552, 1181, 1218, 1219, 1284, 1350, 1915
PstI	CTGCAG	1 cut(s)	2395
PstNI	CAGNNNCTG	1 cut(s)	1610
PsuI	RGATCY	3 cut(s)	1011, 1985, 2326
RsaI	GTAC	9 cut(s)	355, 738, 905, 924, 1384, 1526, 1938, 2022, 2147
RsaNI	GTAC	9 cut(s)	354, 737, 904, 923, 1383, 1525, 1937, 2021, 2146
RseI	CAYNNNNRTG	5 cut(s)	1111, 1158, 1760, 2407, 2413
SalI	GTCGAC	2 cut(s)	584, 1571
SapI	GCTCTTC	2 cut(s)	237, 328
SaqAI	TTAA	7 cut(s)	879, 1368, 1688, 1826, 1982, 2196, 2214
SatI	GCNGC	9 cut(s)	15, 359, 362, 374, 1059, 1482, 1485, 1602, 2391
Sau96I	GGNCC	7 cut(s)	552, 1181, 1218, 1219, 1284, 1350, 1915
ScaI	AGTACT	2 cut(s)	2022, 2147
SchI	GAGTC	5 cut(s)	321, 358, 1400, 1426, 2155
ScrFI	CCNGG	4 cut(s)	88, 366, 1180, 2060
SduI	GDGCHC	3 cut(s)	249, 1222, 2060
SfaNI	GCATC	2 cut(s)	1823, 1884
SfcI	CTRYAG	2 cut(s)	2050, 2391
SinI	GGWCC	3 cut(s)	552, 1350, 1915
SmiMI	CAYNNNNRTG	5 cut(s)	1111, 1158, 1760, 2407, 2413
SmlI	CTYRAG	5 cut(s)	878, 1412, 1661, 1981, 2355
SmoI	CTYRAG	5 cut(s)	878, 1412, 1661, 1981, 2355
SpeI	ACTAGT	1 cut(s)	1460
Sse9I	AATT	9 cut(s)	93, 480, 487, 773, 1050, 1078, 1248, 1338, 2014
SspI	AATATT	1 cut(s)	2319
SspMI	CTAG	6 cut(s)	1008, 1016, 1037, 1461, 1857, 2028
StyD4I	CCNGG	4 cut(s)	86, 364, 1178, 2058
StyI	CCWWGG	2 cut(s)	341, 1015
TaaI	ACNGT	7 cut(s)	11, 579, 590, 736, 1864, 1919, 1975
TaiI	ACGT	4 cut(s)	746, 2097, 2214, 2241
TaqI	TCGA	8 cut(s)	28, 434, 446, 560, 585, 975, 1572, 2304
TaqII	GACCGA	1 cut(s)	415
TasI	AATT	9 cut(s)	93, 480, 487, 773, 1050, 1078, 1248, 1338, 2014
TatI	WGTACW	5 cut(s)	922, 1524, 1936, 2020, 2145
TauI	GCSGC	5 cut(s)	361, 364, 376, 1061, 1487
TfiI	GAWTC	9 cut(s)	62, 148, 280, 503, 1122, 1135, 1142, 1209, 2072
Tru1I	TTAA	7 cut(s)	879, 1368, 1688, 1826, 1982, 2196, 2214
Tru9I	TTAA	7 cut(s)	879, 1368, 1688, 1826, 1982, 2196, 2214
TscAI	CASTG	6 cut(s)	14, 128, 158, 1194, 1680, 2174
TseFI	GTSAC	2 cut(s)	166, 823
TseI	GCWGC	4 cut(s)	14, 1481, 1601, 2390
Tsp45I	GTSAC	2 cut(s)	166, 823
TspGWI	ACGGA	4 cut(s)	219, 308, 402, 996
TspRI	CASTG	6 cut(s)	14, 128, 158, 1194, 1680, 2174
Vha464I	CTTAAG	2 cut(s)	878, 1981
VpaK11BI	GGWCC	3 cut(s)	552, 1350, 1915
VspI	ATTAAT	1 cut(s)	1368
XapI	RAATTY	5 cut(s)	93, 1078, 1248, 1338, 2014
XceI	RCATGY	1 cut(s)	1801
XmaJI	CCTAGG	1 cut(s)	1015
XmiI	GTMKAC	2 cut(s)	585, 1572
XmnI	GAANNNNTTC	1 cut(s)	2003
XspI	CTAG	6 cut(s)	1008, 1016, 1037, 1461, 1857, 2028
ZrmI	AGTACT	2 cut(s)	2022, 2147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.