pycom15g21640

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005287
Physical Location & Seq
Forward (+)
15691613 .. 15692579
967 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 402 bp
ATGTACGAAAAGGGTCCTTTTGATATCTTTTCTGTTGGGGGAGATATGTCGGATGCAAATGTTGTCAAGTTCAGTAACCATGGGAGACTTGTGCTTTCAACAACCACAGGTGGACATATTCATGTCCTTGATTCATTCCGTGGAACACTATTATCCACGTACAATGTTAAGCGAGTCTCCAGCAATTCAACATTAGAGGCTTCTTTTAGCCCCGAGGGGATGTTTGTCATGTCAGGTTCAGGTGATTGTAGTGTCTATGCTTGGAGTGTTCGGTGCGGGAAAGAAGTCACAAGCTGGAAGAGCACCGAAAATGAACCTCCAGTTATCAAATGGGCTCCCGGAAACCTCATGTTTGCAACTGGATCGATCCAAACTATCATTTTGGATTCCAGATTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
WD40

Protein Analysis

134

Amino Acids

14.47

Weight (kDa)

6.26

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 17 - 124 7.1e-11 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 17 - 125 1.6e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 18 - 92 6.5e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 19 - 121 3.1e-08 THOC3 beta-propeller domain
EIF3I PF24805 20 - 102 2.9e-06 EIF3I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 276
AclWI GGATC 2 cut(s) 361, 370
AfaI GTAC 2 cut(s) 5, 161
AgsI TTSAA 2 cut(s) 99, 189
AluBI AGCT 1 cut(s) 294
AluI AGCT 1 cut(s) 294
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 2 cut(s) 79, 181
AlwI GGATC 2 cut(s) 361, 370
Ama87I CYCGRG 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 1 cut(s) 254
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 305
BcgI CGANNNNNNTGC 2 cut(s) 345, 379
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 2 cut(s) 79, 181
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 14
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 2 cut(s) 15, 336
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 1 cut(s) 43
BpmI CTGGAG 2 cut(s) 163, 303
BpuMI CCSGG 1 cut(s) 339
Bsa29I ATCGAT 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 79, 139, 213
Bse1I ACTGG 2 cut(s) 320, 364
BseCI ATCGAT 1 cut(s) 365
BseDI CCNNGG 3 cut(s) 79, 139, 213
BseGI GGATG 2 cut(s) 58, 225
BseNI ACTGG 2 cut(s) 320, 364
BshVI ATCGAT 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 305
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 1 cut(s) 339
BsmAI GTCTC 2 cut(s) 79, 181
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 305, 337
Bsp143I GATC 2 cut(s) 362, 366
Bsp19I CCATGG 1 cut(s) 79
BspACI CCGC 1 cut(s) 276
BspDI ATCGAT 1 cut(s) 365
BspLI GGNNCC 2 cut(s) 15, 336
BspPI GGATC 2 cut(s) 361, 370
BspQI GCTCTTC 1 cut(s) 293
BsrI ACTGG 2 cut(s) 320, 364
BssECI CCNNGG 3 cut(s) 79, 139, 213
BssMI GATC 2 cut(s) 362, 366
BssT1I CCWWGG 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 159
BstDSI CCRYGG 2 cut(s) 79, 139
BstF5I GGATG 2 cut(s) 58, 225
BstKTI GATC 2 cut(s) 365, 369
BstMAI GTCTC 2 cut(s) 79, 181
BstMBI GATC 2 cut(s) 362, 366
BstMWI GCNNNNNNNGC 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 337
Bsu15I ATCGAT 1 cut(s) 365
BsuTUI ATCGAT 1 cut(s) 365
BtgI CCRYGG 2 cut(s) 79, 139
BtsCI GGATG 2 cut(s) 58, 225
Cfr13I GGNCC 1 cut(s) 14
ClaI ATCGAT 1 cut(s) 365
Csp6I GTAC 2 cut(s) 4, 160
CviAII CATG 4 cut(s) 80, 122, 229, 349
CviJI RGCY 4 cut(s) 200, 210, 294, 335
CviKI_1 RGCY 4 cut(s) 200, 210, 294, 335
CviQI GTAC 2 cut(s) 4, 160
DpnI GATC 2 cut(s) 364, 368
DpnII GATC 2 cut(s) 362, 366
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 337
Eco32I GATATC 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 14
EcoRV GATATC 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 79
FaeI CATG 4 cut(s) 83, 125, 232, 352
FaiI YATR 7 cut(s) 47, 81, 117, 123, 230, 258, 350
FatI CATG 4 cut(s) 79, 121, 228, 348
FauI CCCGC 1 cut(s) 269
FokI GGATG 2 cut(s) 65, 232
FriOI GRGCYC 1 cut(s) 337
GsuI CTGGAG 2 cut(s) 163, 303
HapII CCGG 1 cut(s) 339
Hin1II CATG 4 cut(s) 83, 125, 232, 352
HinfI GANTC 3 cut(s) 131, 174, 386
HpaII CCGG 1 cut(s) 339
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 1 cut(s) 113
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 1 cut(s) 390
Hpy8I GTNNAC 1 cut(s) 113
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 56, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 300
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 4 cut(s) 83, 125, 232, 352
Kzo9I GATC 2 cut(s) 362, 366
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 8 cut(s) 93, 193, 219, 225, 280, 333, 345, 352
LweI GCATC 1 cut(s) 43
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 2 cut(s) 74, 286
MalI GATC 2 cut(s) 364, 368
MboI GATC 2 cut(s) 362, 366
MboII GAAGA 1 cut(s) 310
MhlI GDGCHC 2 cut(s) 305, 337
MluCI AATT 1 cut(s) 184
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 4 cut(s) 190, 208, 327, 356
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 1 cut(s) 120
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 300
NciI CCSGG 1 cut(s) 339
NcoI CCATGG 1 cut(s) 79
NdeII GATC 2 cut(s) 362, 366
NlaIII CATG 4 cut(s) 83, 125, 232, 352
NlaIV GGNNCC 2 cut(s) 15, 336
NmuCI GTSAC 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 131, 386
PfoI TCCNGGA 1 cut(s) 337
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Ppu21I YACGTR 1 cut(s) 159
PpuMI RGGWCCY 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 14
PspN4I GGNNCC 2 cut(s) 15, 336
PspPI GGNCC 1 cut(s) 14
PspPPI RGGWCCY 1 cut(s) 14
RsaI GTAC 2 cut(s) 5, 161
RsaNI GTAC 2 cut(s) 4, 160
RseI CAYNNNNRTG 1 cut(s) 120
SapI GCTCTTC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 168
Sau3AI GATC 2 cut(s) 362, 366
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 2 cut(s) 305, 337
SetI ASST 7 cut(s) 112, 161, 238, 244, 296, 319, 348
SfaNI GCATC 1 cut(s) 43
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 120
Sse9I AATT 1 cut(s) 184
SsiI CCGC 1 cut(s) 276
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 79
TaiI ACGT 1 cut(s) 161
TaqI TCGA 1 cut(s) 365
TasI AATT 1 cut(s) 184
TfiI GAWTC 2 cut(s) 131, 386
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseFI GTSAC 1 cut(s) 286
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 3 cut(s) 110, 123, 327
TspGWI ACGGA 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.