pycom15g25630

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009888
Physical Location & Seq
Forward (+)
20125880 .. 20128494
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 876 bp
ATGAGACCCAAAAGTGGGAAAGGGAATTGGAGCTCCAAGTTCAAGTTCAAGGGCATACTCGGATTGCCCACCACTTTGATCTTCTGCTCCTTCTTCTTCCTTGCTGGCTTCTATGGCTCCTCCCTCCTCTCCCAGCATGTGAGTGGTGGTGGTAATAGGCTGAGAGCGAGACTGTTGGAGACAGAGTACAATTCGATGCCTTATGGCAAGACCGGTGACGATTCTTTAACTTCCATTCCCTTTCAGGTTTTGAGTTGGTATCCGCGAGCATTATATTTTCCTAATTTTGCAACTGCGGAGCAATGCGAAAGCATAATCGGTTTGGCAAAACCACGCCTTAAACCATCGATTTTGGCTTTGCGAGAAGGAGAGACAGAGGAAAGCACAAAGGATATACGAACAAGTTCAGGCGTGTTTCTTTCTGCTTCTGATGATAAATCCGGGACTTTGGATGTGATTGAGGAAAAGATTGCAAGGGCTACAATGCTTCCCAGGACACACGGAGAGGCATTTAACGTCTTGCGCTATGAGATCGGGCAGAAGTATAATTCTCATTATGATGCATTCCACCCTGATCAATATGGTCCTCAGAAGAGCCAAAGGGTTGCATCTTTCTTGTTGTATTTAAGTGATGTTCAAGAAGGTGGTGAAACCATGTTTCCTTACGAGAATGGCTCAAACACGGATGGAACCTATGATTTTCGGGAATGTGTTGGTTTAAAAGTGAAGCCACGCAAAGGGGATGGACTTCTGTTTTATTCATTGCTTCCAAATGGTACAATTGATATGTTAGCATTACACGGGAGCTGCCCTGTAATCGAAGGGGAAAAATGGGTAGCTACAAAGTGGATCAGGGACCAAGAACAAGAGGATTAG

Protein Analysis

292

Amino Acids

32.55

Weight (kDa)

6.25

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 172 - 285 2.4e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 265
AciI CCGC 2 cut(s) 263, 296
AclWI GGATC 1 cut(s) 857
AfaI GTAC 2 cut(s) 188, 778
AfiI CCNNNNNNNGG 3 cut(s) 14, 15, 737
AgeI ACCGGT 1 cut(s) 212
AgsI TTSAA 3 cut(s) 43, 49, 638
AjnI CCWGG 1 cut(s) 491
AjuI GAANNNNNNNTTGG 2 cut(s) 29, 61
AluBI AGCT 3 cut(s) 33, 807, 839
AluI AGCT 3 cut(s) 33, 807, 839
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 3 cut(s) 163, 173, 365
AlwI GGATC 1 cut(s) 857
ApeKI GCWGC 1 cut(s) 807
AsiGI ACCGGT 1 cut(s) 212
Asp700I GAANNNNTTC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 525
AspS9I GGNCC 2 cut(s) 584, 856
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 227, 659
AvaII GGWCC 2 cut(s) 584, 856
BanII GRGCYC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 794
BccI CCATC 3 cut(s) 352, 680, 737
BciT130I CCWGG 1 cut(s) 493
BciVI GTATCC 1 cut(s) 270
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 163, 173, 365
BfuI GTATCC 1 cut(s) 270
BisI GCNGC 1 cut(s) 808
BlsI GCNGC 1 cut(s) 809
Bme1390I CCNGG 2 cut(s) 442, 493
Bme18I GGWCC 2 cut(s) 584, 856
BmgT120I GGNCC 2 cut(s) 584, 856
BmiI GGNNCC 3 cut(s) 118, 691, 857
BmrFI CCNGG 2 cut(s) 442, 493
BmsI GCATC 3 cut(s) 186, 550, 617
BplI GAGNNNNNCTC 2 cut(s) 659, 691
BpuMI CCSGG 1 cut(s) 442
Bsa29I ATCGAT 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 491
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 15, 737
Bse118I RCCGGY 1 cut(s) 212
Bse3DI GCAATG 2 cut(s) 308, 761
BseBI CCWGG 1 cut(s) 493
BseCI ATCGAT 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 3 cut(s) 457, 691, 748
BseLI CCNNNNNNNGG 3 cut(s) 14, 15, 737
BseMI GCAATG 2 cut(s) 308, 761
BseMII CTCAG 2 cut(s) 152, 602
BseRI GAGGAG 2 cut(s) 109, 116
BseXI GCAGC 1 cut(s) 794
BseYI CCCAGC 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 265
BshTI ACCGGT 1 cut(s) 212
BshVI ATCGAT 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 2 cut(s) 213, 441
BslFI GGGAC 2 cut(s) 457, 869
BslI CCNNNNNNNGG 3 cut(s) 14, 15, 737
BsmAI GTCTC 3 cut(s) 163, 173, 365
BsmFI GGGAC 2 cut(s) 457, 869
BsmI GAATGC 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 4 cut(s) 78, 531, 574, 849
BspACI CCGC 2 cut(s) 263, 296
BspCNI CTCAG 2 cut(s) 153, 601
BspDI ATCGAT 1 cut(s) 347
BspFNI CGCG 1 cut(s) 265
BspLI GGNNCC 3 cut(s) 118, 691, 857
BspPI GGATC 1 cut(s) 857
BspQI GCTCTTC 1 cut(s) 587
BsrDI GCAATG 2 cut(s) 308, 761
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 4 cut(s) 78, 531, 574, 849
Bst2UI CCWGG 1 cut(s) 493
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 106, 267
BstDEI CTNAG 2 cut(s) 161, 588
BstF5I GGATG 3 cut(s) 457, 691, 748
BstFNI CGCG 1 cut(s) 265
BstHHI GCGC 1 cut(s) 525
BstKTI GATC 4 cut(s) 81, 534, 577, 852
BstMAI GTCTC 3 cut(s) 163, 173, 365
BstMBI GATC 4 cut(s) 78, 531, 574, 849
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNI CCWGG 1 cut(s) 493
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 440, 491
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 1 cut(s) 794
Bsu15I ATCGAT 1 cut(s) 347
BsuI GTATCC 1 cut(s) 270
BsuTUI ATCGAT 1 cut(s) 347
BtsCI GGATG 3 cut(s) 457, 691, 748
Cac8I GCNNGC 2 cut(s) 106, 267
CfoI GCGC 1 cut(s) 525
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 584, 856
ClaI ATCGAT 1 cut(s) 347
Csp6I GTAC 2 cut(s) 187, 777
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 2 cut(s) 137, 655
CviQI GTAC 2 cut(s) 187, 777
DdeI CTNAG 2 cut(s) 161, 588
DpnI GATC 4 cut(s) 80, 533, 576, 851
DpnII GATC 4 cut(s) 78, 531, 574, 849
DraI TTTAAA 1 cut(s) 720
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
Ecl136II GAGCTC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 35
Eco47I GGWCC 2 cut(s) 584, 856
Eco53kI GAGCTC 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 491
EcoT22I ATGCAT 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 2 cut(s) 140, 658
FaqI GGGAC 2 cut(s) 457, 869
FatI CATG 2 cut(s) 136, 654
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 1 cut(s) 808
FokI GGATG 3 cut(s) 464, 698, 755
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 1 cut(s) 808
GlaI GCGC 1 cut(s) 524
GluI GCNGC 1 cut(s) 808
GsaI CCCAGC 1 cut(s) 136
HapII CCGG 2 cut(s) 213, 441
HhaI GCGC 1 cut(s) 525
Hin1II CATG 2 cut(s) 140, 658
Hin6I GCGC 1 cut(s) 523
HinP1I GCGC 1 cut(s) 523
HinfI GANTC 1 cut(s) 221
HpaII CCGG 2 cut(s) 213, 441
HphI GGTGA 2 cut(s) 227, 659
Hpy188I TCNGA 3 cut(s) 62, 430, 591
Hpy188III TCNNGA 2 cut(s) 638, 704
HpyAV CCTTC 4 cut(s) 100, 359, 635, 815
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 4 cut(s) 290, 473, 563, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 2 cut(s) 161, 588
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 2 cut(s) 140, 658
HspAI GCGC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 4 cut(s) 78, 531, 574, 849
LguI GCTCTTC 1 cut(s) 587
LmnI GCTCC 6 cut(s) 30, 38, 92, 122, 298, 804
Lsp1109I GCAGC 1 cut(s) 794
LweI GCATC 3 cut(s) 186, 550, 617
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 1 cut(s) 215
MalI GATC 4 cut(s) 80, 533, 576, 851
MboI GATC 4 cut(s) 78, 531, 574, 849
MboII GAAGA 4 cut(s) 73, 85, 88, 604
MfeI CAATTG 1 cut(s) 780
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 5 cut(s) 25, 190, 283, 547, 780
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 8 cut(s) 130, 134, 137, 370, 454, 499, 597, 862
Mph1103I ATGCAT 1 cut(s) 565
MroXI GAANNNNTTC 1 cut(s) 403
MseI TTAA 5 cut(s) 227, 339, 513, 626, 719
MslI CAYNNNNRTG 2 cut(s) 141, 558
MspI CCGG 2 cut(s) 213, 441
MspR9I CCNGG 2 cut(s) 442, 493
MunI CAATTG 1 cut(s) 780
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 1 cut(s) 493
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 1 cut(s) 114
NciI CCSGG 1 cut(s) 442
NdeII GATC 4 cut(s) 78, 531, 574, 849
NlaIII CATG 2 cut(s) 140, 658
NlaIV GGNNCC 3 cut(s) 118, 691, 857
NmuCI GTSAC 1 cut(s) 215
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 587
PctI GAATGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 403
PfeI GAWTC 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 440
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 1 cut(s) 809
Psp124BI GAGCTC 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 1 cut(s) 491
PspN4I GGNNCC 3 cut(s) 118, 691, 857
PspPI GGNCC 2 cut(s) 584, 856
RsaI GTAC 2 cut(s) 188, 778
RsaNI GTAC 2 cut(s) 187, 777
RseI CAYNNNNRTG 2 cut(s) 141, 558
SacI GAGCTC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 587
SaqAI TTAA 5 cut(s) 227, 339, 513, 626, 719
SatI GCNGC 1 cut(s) 808
Sau3AI GATC 4 cut(s) 78, 531, 574, 849
Sau96I GGNCC 2 cut(s) 584, 856
ScrFI CCNGG 2 cut(s) 442, 493
SduI GDGCHC 1 cut(s) 35
SetI ASST 7 cut(s) 35, 249, 519, 646, 695, 809, 841
SfaNI GCATC 3 cut(s) 186, 550, 617
SinI GGWCC 2 cut(s) 584, 856
SmiMI CAYNNNNRTG 2 cut(s) 141, 558
Sse9I AATT 5 cut(s) 25, 190, 283, 547, 780
SsiI CCGC 2 cut(s) 263, 296
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 440, 491
TaaI ACNGT 1 cut(s) 174
TaiI ACGT 1 cut(s) 519
TaqI TCGA 3 cut(s) 194, 347, 819
TasI AATT 5 cut(s) 25, 190, 283, 547, 780
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 5 cut(s) 227, 339, 513, 626, 719
Tru9I TTAA 5 cut(s) 227, 339, 513, 626, 719
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 1 cut(s) 807
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 750
TspGWI ACGGA 2 cut(s) 516, 698
VpaK11BI GGWCC 2 cut(s) 584, 856
XceI RCATGY 1 cut(s) 140
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.