Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Reverse (-)

23724992 .. 23731354

Gene Structure 6363 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5157 bp

ATGAGATCATCGTACCCGCGCACTACTTCCCGGCAACCTCAGCCGTCGGGAATTCCCCGCCGTTTGCCTCCCCACAGTTACCCGCCCAACAACTTCTACCGCCAAACCCCTCCTCCCTCCCGCACAAGCTTCATCGTCGAGCTACTCGCCGATCACCGGAGTCTCCGAAAGCATGAAATCGACGCCGTGATCGCTAACTGCAAGGCCAGGCCCGAACGAGTTGAGGTTTCTCCAGCCAGTCGCATCGTGGCCTCCTTGTTCTACACGCAATGGGTCGATGCGCTAGAAGCCCTCGTTTATTTTTGGGAGTCCCGGCTGGACCGGTTTCATGATCTCACTCCTAAGTTGAACCGAATCGTCTCTGTTGCCTCCGATCTCGACGAGCTCCGGGACCGGCTGAAAGCCCTGTTCTCCGGCCGGATAAAGAAATTGATGGACGGAGAGGCGGTGAAGAAGTGGGAGGCGAAGCGAGTTTGGCTGTGCAACGACAACGAACGAGTTTCCAAGTTGCTCCGGCGGCATAACCATCTGTGGAGTTTCAGCGAGTTGAATGAGACACTAAAGAGGAACAGGAGCGAAATTCAATTGGTTGAGAGCAGGATTAGGGAGTTTAAGTCCGCCATGAATTGCTTGCTTGATTATCTTGAGGGGAAAGGGGCGGAGGAATCGGGTGAAGAAGGGGTGAAGGTGTTCAAATTTTCGAGTGAAGTTTATGATTGGAGTCAAATTCACAGCTTAATAGTTCGGGAATGCCGCAGGCTCGAAGAAGGGTTGCCCATTTACGCTTATCGGCAGCAAATTCTACAGCAAATTCTTACCCAGCAGGTCACGGTATTGATTGGAGAGACTGGCTCAGGGAAAAGTACACAGCTGGTGCAGTTTCTTGCCGATTCTGGAATTGCTGCCGAGCAGTCCATTGTATGCACTCAGCCTCGAAAGATTGCTGCCATGTCGTTGTCGCAGAGAGTTAGAGAAGAAAGTAATGGGTGTTACGGAGGAAAATCAGTATCTTGTAATTCAACATTTTTGTCAGGGCAGCAGTTGGTTTCGAAGGTGACATTTATGACAGATCACTATTTACTGCAGCATTACATGAATGATACTAAGATGTCTGGTATTTCTTGCATAATAGTCGATGAGGCTCACGAAAGGAGCCTGAATACTGATCTCCTGTTGGCATTGTTAAAAGATTTACTTGGTCGACGGTCTAGTTTACGACTTATTATAATGTCTGCAACAGCTGATGCCAATGTGCTTTCCGAATATTTTTATCATTGTGGAATCTTTTATGTATTGGGGAGGAGCTTTCGCGTTGATGTTAGATATGTACCATCTTTCACTGAAGGAACTTCTAGTGATGCTTCCTATGTTTCTGATGTGATGAGGGTAGCAACAGATGTACACAGAAAAGAGAAAGAGGGGACGATTCTTGCCTTTTTGACTTCCCAGTTGGAAGTAGAATGGTGTTGTGATAAGTTTATAGCACCACGTGCAATTGCATTGCCCTTGCATGGAAAACTTTCTTATGAAGATCAATTTAATGTTTTCCAAAACTACCCTGGAAAAAGGAAAATCATATTCGCCACTAATCTGGCTGAGACATCTCTGACAATTCCTGGGGTAAAGTATGTGATTGATTCTGGTATGGCAAAAGAAAGCAAATTTGAACCGGGAAGTGGTATGAATGTTCTGAGAGTTTGTATGATCAGCCAGAGTTCTGCTAATCAAAGAAGTGGCCGTGCTGGAAGAACTGAACCAGGAGTATGTTATAGACTTTACTCAGAATATGACTTTGAGGCTATGCCTCCATGTCAGGAACCTGAGATTCGCAGAGTTCATCTTGGTGTTGCAGTTCTGAGGATTCTGGCTTTAGGCGTCAAGAATTTACATGACTTTAAATTTATTGATGCACCTTCTTCTGAAGCTATTGACTTGGCTATCAGGAACCTTGTTCAGTTGGGAGCTGTTAAGCAAAAGAATGGTGTTTTTGAGTTAACCCAGGAAGGTCGGTGCTTGGTTAAATTGGGTGTTGAGCCTCGGCTTGGAAAATTAATTCTTGGTTGTTGTGACCTTAACTTGCGTATGGAGGGTCTTGTTCTTGCAGCTGTAATGGCAAATTCCCGCAGCATATTTTGTAGAGTTGGTAATGATGAAGAAAAGCTTAGATCAGACTGCATCAAGGTGAAATTTTGCCATCGTGATGGTGACCTCTTTACCCTTCTCTCTGTGTACAAGGAATGGGATTCTTTGTCTCGAGACGAGAGAAATGTGTGGTGCTGGGAAAACAGCATCAATGCCAAAACAATGAGGAGATGCCAAGACACGGTGATGGAATTGGAGTCTTGCCTTAAACATGAACTCCACATGATAATTTCTGGTACCTGGCGTTGGGATCCACGTGAATCAAATGATTGTGACAAACATTTGAAAAAGGTTATACTTTCTTCCCTTGCAGATAATGTTGCTATGTTCTCAGGTTATGATCAACTTGGTTACGAAGTGGCATTAACTGGGCAGCATGTTAGGCTGCATCCTTCGTGTTCATTGCTAGTATTTGGGGAGAAACCTAGTTGGGTAGTGTTTGGTGAAATCCTGTCGGTTTCTAATCAGTATCTGGTGTGTGTCACTTCCTTTGATTTCAACTCTTTGTCTGCGCTTTATCCTCCTCCTTCATTTGATGTATCAAAGATGGAAAGTCGAAAGCTTCAGCTGAAAGTGTTGACTGGTTTTGGCATTACTGTTCTTAAAAGATTTTGTGGGAAGGCAAACAGTTATCTGCTTCATCTCGTGTCCCGTATTAGGACAATTTGTGAGGATGAACTTATCAACATTAAAGTCGATTACTATCAGAATGAGATTATGTTGTTTGCCACCTCAAATAACATGGATAGGGTTGAAAACTTTGTAAATAATGCCTTGGAGTGTGAAAAAAAATGGATGCGTAATGAATGCTTGGAGAAATGCTTATATCACAGTTCTGGCGTCTTGCCTCCTGTAGCATTGTTTGGAGCCGGTGCAGAGATCAAACATCTGGAGCTTCAAAAAAGGTGTTTGACATTTAATGTAATTCATTCAAAACTGGATTCTGAAGATGATAAGGAGTTACTTAGTGAGCTTGAGAAGTCTATCTCTGGTGGTATATGTGCTATTCACAAGTTCCCCAGCACTGGGCAGGAAAGTGTTGACAAAGGAAAATGGGTGAGGGTAACATTTCAAACTCCTGAAGCGGCTCAAAAAGCAGCTGAGCTAAACGAATCTGAATTTAGTGGCTCCATACTGAAGGTTATTCCTTCGCAGATCGATCATAAAATATTTTCATTTCCCGCTGTTAGAGCTAAGGTTTATTGGCCTCGCAGGCTAAGCAAGGGGTTTGCAATCGTCAAATGCCACATGAATGATATCTATCCCATAATTGATGACTTTTCTAACCTTGAAGTTGGTGGAAAAAAAATTCGCTGTGAGATTAGCAAAAAAAGGGACATGGATGCTATCGTGATATATGGACTAAGCAAAGATCTGTCCGAGGCTGAAATTTTGGACGTGTTGAGGACTGCTACAAGAAGGCAAATCCTTGATTTTTTTCTGCTGAGAGGAGATGCTGTAGGAAATCCGCCATGTAGTTTTTGTGAAGAAGCACTCTTAAAAGAAATATCACCTTTTATGCCTAAGAGGTATTCCCACAATAGCTGCAGAGTTCAGGTTTTCGAGCCTGAACCAAAGAGTGTTTTCATGAGAGCTTTGATTACATTTGATGGAAGATTACACTTGGAAGCGGCGAGAGCTTTAGAAGAATTAGAAGGGAAGGTACTGCCTGGGTTCCTTCCATGGCAAAAGATAAAATGCCAGCAGTTGTTTCATAGCTCTCTATCCTGCCCCGCCCCTGTCTATCTTGTGATCAAGAAGCAGCTGGATACTTTACTCTCAAGTTTTATACCTCTAAATGGTGTAGAATGCAGCTTAGATAGGAATGCTAATGGTTCCTACCGAGTGAAGATATCTGCCACTGCTACAAAAGTTGTTGCAGAACTGAGAAGACGTGTGGAAGAGCTTGTTAAAGGAAAGACTATAGACCATGCCAGCCTTACTCCAGCTGTTTTACAGCTTCTTTTCTCTAGGGATGGCATCAGTCTAATGCACGCACTCCAGCGAGAGACAGGAACCTATATTCTTTTTGACAGACGTAGCCTCAGTGTACAGGTCTTTGGTTCTTCAGACCAAGTTGGTGTGGTAGAGAAGAAATTGGTCGATTCCCTTTTAACCCTCCATGACAATAAGCTGCTTGAGATCCGACTTCAAGGCCATACCTTACCTCCCGAGTTGATGAAAGAGGTTGTTAACAGGTTTGGGCCAGACTTACGGGGGCTCAAAGAGAAGATCCCAGGGGCTGATTTTGGTCTAAATGTTCGCCGTCAAGTAATTTCCATCCATGGAAGCAAGGATGTGAAGCAGAAAGTAGAAGAGAGTATTTATGAGATTGTACAGACGTCAGGTAGTTCAACTCAGAGGTTTAAGAGTGATGTTAATTGCCCCATATGTATGTGTGAGATTGAAGATGAATACCGGCTCGAGGACTGTGGGCATTTATTTTGCCGCTCGTGTTTAGTGGAGCAGTGCGAGTCGGCAATTAAGAACCAAGATAGTTTCCCAATGTTTTGTGCACACGAGGGTTGCAGATCTCTTATTTTGTTCATTGATCTGAAGTCTCTATTATCAAATGAGAAGCTGGAGGAGCTATTTAGGGCTTCACTGGGGGCGTTTGTTGCATCAAGTGCAGGTATCTACAGGTTTTGCCCGTCTCCTGACTGCTCTTCTGTGTATCAAGTAGCTGCTCCTGGGACAGACGGTGAACCATTTGTTTGTGGTGCATGTTATGCCGAGACATGTACCAGGTGCCATTTAGAACATCATCCATACCTCTCATGTGAGCAGTACCGGGAGTTCAAGGAAGACCCAGATTCATCGTTGAAGCAGTGGTGCAAAGGAAAAGAACATGTGAAGAGCTGCCCAGTATGCAGGTATACAATTGAGAAGATAGACGGGTGCAACCATATAGAATGTAGGTGTGGGAAGCACATTTGCTGGGCCTGTTTGGAGTATTATGCAACGAGCGACGAATGCTATACCCATTTGAGGACTATTCACCTGGCCATCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1719

Amino Acids

194.7

Weight (kDa)

7.87

Isoelectric Point (pI)

47.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	263 - 418	1.9e-10	DEAD/DEAH box helicase
Helicase_C	PF00271	466 - 584	2.1e-13	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	809 - 883	1.6e-09	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	985 - 1099	3.5e-47	DEAH11-like, RNA-binding domain
RRM_DEAH11	PF24637	1117 - 1198	1.2e-34	DEAH11 RRM domain
KH_DEAH11_1st	PF24638	1287 - 1351	1.9e-30	DEAH11 eukaryotic type KH-domain
KH_DEAH11_2nd	PF24641	1352 - 1425	1.7e-35	DEAH11 eukaryotic type KH-domain
KH_DEAH11	PF24471	1427 - 1495	1.4e-29	DEAH11-like, KH-domain
IBR	PF01485	1584 - 1641	4.1e-08	IBR domain, a half RING-finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1226
AatII	GACGTC	1 cut(s)	4487
Acc36I	ACCTGC	3 cut(s)	814, 4763, 5004
Acc65I	GGTACC	1 cut(s)	2378
AccB1I	GGYRCC	2 cut(s)	2378, 4890
AccBSI	CCGCTC	1 cut(s)	4593
AccI	GTMKAC	2 cut(s)	1201, 5018
AccII	CGCG	2 cut(s)	19, 1311
AclWI	GGATC	4 cut(s)	2387, 2400, 4279, 4369
AcoI	YGGCCR	3 cut(s)	415, 1735, 5145
AcuI	CTGAAG	8 cut(s)	1362, 1941, 2690, 3108, 3243, 3299, 4194, 4718
AcvI	CACGTG	2 cut(s)	1490, 2399
AcyI	GRCGYC	4 cut(s)	183, 1875, 2982, 4484
AdeI	CACNNNGTG	1 cut(s)	1490
AflIII	ACRYGT	4 cut(s)	3540, 4036, 4880, 4990
AgeI	ACCGGT	1 cut(s)	321
AjiI	CACGTC	2 cut(s)	3541, 4037
Alw21I	GWGCWC	2 cut(s)	387, 4660
Alw44I	GTGCAC	1 cut(s)	4656
AlwI	GGATC	4 cut(s)	2387, 2400, 4279, 4369
AlwNI	CAGNNNCTG	2 cut(s)	2614, 4385
Ama87I	CYCGRG	3 cut(s)	2253, 4313, 4565
ApaLI	GTGCAC	1 cut(s)	4656
AseI	ATTAAT	1 cut(s)	2051
AsiGI	ACCGGT	1 cut(s)	321
Asp700I	GAANNNNTTC	2 cut(s)	689, 1519
Asp718I	GGTACC	1 cut(s)	2378
AspLEI	GCGC	3 cut(s)	21, 283, 2656
AspS9I	GGNCC	5 cut(s)	210, 319, 391, 4346, 5082
AsuC2I	CCSGG	5 cut(s)	31, 313, 389, 1671, 4934
AsuII	TTCGAA	1 cut(s)	1049
AvaI	CYCGRG	3 cut(s)	2253, 4313, 4565
AvaII	GGWCC	2 cut(s)	319, 391
BaeGI	GKGCMC	1 cut(s)	4660
BalI	TGGCCA	1 cut(s)	5147
BamHI	GGATCC	1 cut(s)	2392
BanI	GGYRCC	2 cut(s)	2378, 4890
BanII	GRGCYC	2 cut(s)	387, 4365
BarI	GAAGNNNNNNTAC	2 cut(s)	3789, 3821
BauI	CACGAG	3 cut(s)	2786, 4594, 4661
BbrPI	CACGTG	2 cut(s)	1490, 2399
BbsI	GAAGAC	2 cut(s)	4039, 4953
Bbv12I	GWGCWC	2 cut(s)	387, 4660
BbvCI	CCTCAGC	1 cut(s)	39
BceAI	ACGGC	5 cut(s)	28, 45, 170, 1722, 4392
BcgI	CGANNNNNNTGC	2 cut(s)	1114, 1148
BciVI	GTATCC	1 cut(s)	3904
BclI	TGATCA	3 cut(s)	1704, 2482, 3894
BcnI	CCSGG	5 cut(s)	31, 313, 389, 1671, 4934
BfaI	CTAG	6 cut(s)	284, 1209, 1353, 2549, 2568, 4113
BfmI	CTRYAG	7 cut(s)	803, 1082, 2994, 3600, 3688, 4065, 4780
BfuAI	ACCTGC	3 cut(s)	814, 4763, 5004
BfuI	GTATCC	1 cut(s)	3904
BglI	GCCNNNNNGGC	1 cut(s)	3355
BglII	AGATCT	2 cut(s)	3514, 4673
BlpI	GCTNAGC	2 cut(s)	3243, 3359
Bme18I	GGWCC	2 cut(s)	319, 391
BmeT110I	CYCGRG	3 cut(s)	2253, 4313, 4565
BmgBI	CACGTC	2 cut(s)	3541, 4037
BmgT120I	GGNCC	5 cut(s)	210, 319, 391, 4346, 5082
BmrI	ACTGGG	5 cut(s)	1441, 2520, 3177, 4757, 5000
BmuI	ACTGGG	5 cut(s)	1441, 2520, 3177, 4757, 5000
BpiI	GAAGAC	2 cut(s)	4039, 4953
BplI	GAGNNNNNCTC	2 cut(s)	836, 868
BpmI	CTGGAG	5 cut(s)	216, 3053, 4071, 4127, 4745
Bpu10I	CCTNAGC	3 cut(s)	39, 853, 3336
Bpu1102I	GCTNAGC	2 cut(s)	3243, 3359
Bpu14I	TTCGAA	1 cut(s)	1049
BpuEI	CTTGAG	4 cut(s)	665, 3137, 3907, 4301
BpuMI	CCSGG	5 cut(s)	31, 313, 389, 1671, 4934
Bsa29I	ATCGAT	1 cut(s)	3300
BsaAI	YACGTR	2 cut(s)	1490, 2399
BsaBI	GATNNNNATC	2 cut(s)	2844, 3402
BsaHI	GRCGYC	4 cut(s)	183, 1875, 2982, 4484
BsaWI	WCCGGW	2 cut(s)	156, 321
Bse118I	RCCGGY	4 cut(s)	321, 393, 3011, 4560
Bse1I	ACTGG	9 cut(s)	237, 853, 1447, 2515, 2728, 3084, 3172, 4752, 5006
Bse3DI	GCAATG	3 cut(s)	275, 1499, 2543
Bse8I	GATNNNNATC	2 cut(s)	2844, 3402
BseCI	ATCGAT	1 cut(s)	3300
BseGI	GGATG	8 cut(s)	2530, 2821, 2943, 3490, 4123, 4422, 4444, 4906
BseJI	GATNNNNATC	2 cut(s)	2844, 3402
BseMI	GCAATG	3 cut(s)	275, 1499, 2543
BseNI	ACTGG	9 cut(s)	237, 853, 1447, 2515, 2728, 3084, 3172, 4752, 5006
BseRI	GAGGAG	6 cut(s)	102, 1315, 2323, 2655, 3606, 4742
BseSI	GKGCMC	1 cut(s)	4660
BseX3I	CGGCCG	1 cut(s)	415
BseYI	CCCAGC	4 cut(s)	819, 2277, 3161, 5079
BsgI	GTGCAG	3 cut(s)	896, 3036, 4791
Bsh1236I	CGCG	2 cut(s)	19, 1311
Bsh1285I	CGRYCG	1 cut(s)	418
BshNI	GGYRCC	2 cut(s)	2378, 4890
BshTI	ACCGGT	1 cut(s)	321
BshVI	ATCGAT	1 cut(s)	3300
BsiEI	CGRYCG	1 cut(s)	418
BsiHKAI	GWGCWC	2 cut(s)	387, 4660
BsiHKCI	CYCGRG	3 cut(s)	2253, 4313, 4565
BslFI	GGGAC	6 cut(s)	295, 404, 1435, 2776, 3491, 4849
BsmBI	CGTCTC	3 cut(s)	364, 2250, 4800
BsmFI	GGGAC	6 cut(s)	295, 404, 1435, 2776, 3491, 4849
BsmI	GAATGC	5 cut(s)	755, 2954, 3956, 3973, 5120
BsoBI	CYCGRG	3 cut(s)	2253, 4313, 4565
Bsp119I	TTCGAA	1 cut(s)	1049
Bsp1286I	GDGCHC	3 cut(s)	387, 4365, 4660
Bsp1407I	TGTACA	4 cut(s)	1399, 2229, 4192, 4477
Bsp1720I	GCTNAGC	2 cut(s)	3243, 3359
Bsp19I	CCATGG	2 cut(s)	3824, 4426
BspDI	ATCGAT	1 cut(s)	3300
BspFNI	CGCG	2 cut(s)	19, 1311
BspHI	TCATGA	2 cut(s)	328, 3729
BspMAI	CTGCAG	2 cut(s)	1086, 3692
BspMI	ACCTGC	3 cut(s)	814, 4763, 5004
BspPI	GGATC	4 cut(s)	2387, 2400, 4279, 4369
BspQI	GCTCTTC	3 cut(s)	4038, 4813, 4991
BspT104I	TTCGAA	1 cut(s)	1049
BspT107I	GGYRCC	2 cut(s)	2378, 4890
BsrBI	CCGCTC	1 cut(s)	4593
BsrDI	GCAATG	3 cut(s)	275, 1499, 2543
BsrFI	RCCGGY	4 cut(s)	321, 393, 3011, 4560
BsrGI	TGTACA	4 cut(s)	1399, 2229, 4192, 4477
BsrI	ACTGG	9 cut(s)	237, 853, 1447, 2515, 2728, 3084, 3172, 4752, 5006
BssAI	RCCGGY	4 cut(s)	321, 393, 3011, 4560
BssNAI	GTATAC	1 cut(s)	5019
BssNI	GRCGYC	4 cut(s)	183, 1875, 2982, 4484
BssSI	CACGAG	3 cut(s)	2786, 4594, 4661
BssT1I	CCWWGG	3 cut(s)	2916, 3824, 4426
Bst1107I	GTATAC	1 cut(s)	5019
Bst2BI	CACGAG	3 cut(s)	2786, 4594, 4661
Bst4CI	ACNGT	9 cut(s)	77, 832, 1206, 2326, 2740, 2771, 2975, 4574, 4844
Bst6I	CTCTTC	4 cut(s)	4038, 4452, 4813, 4991
BstACI	GRCGYC	4 cut(s)	183, 1875, 2982, 4484
BstAPI	GCANNNNNTGC	3 cut(s)	1490, 4769, 4871
BstAUI	TGTACA	4 cut(s)	1399, 2229, 4192, 4477
BstBAI	YACGTR	2 cut(s)	1490, 2399
BstBI	TTCGAA	1 cut(s)	1049
BstC8I	GCNNGC	6 cut(s)	632, 758, 3356, 3845, 4078, 4137
BstDSI	CCRYGG	2 cut(s)	3824, 4426
BstEII	GGTNACC	1 cut(s)	2204
BstF5I	GGATG	8 cut(s)	2530, 2821, 2943, 3490, 4123, 4422, 4444, 4906
BstFNI	CGCG	2 cut(s)	19, 1311
BstHHI	GCGC	3 cut(s)	21, 283, 2656
BstMCI	CGRYCG	1 cut(s)	418
BstNSI	RCATGY	4 cut(s)	2522, 4869, 4884, 4994
BstPI	GGTNACC	1 cut(s)	2204
BstSFI	CTRYAG	7 cut(s)	803, 1082, 2994, 3600, 3688, 4065, 4780
BstSLI	GKGCMC	1 cut(s)	4660
BstUI	CGCG	2 cut(s)	19, 1311
BstV2I	GAAGAC	2 cut(s)	4039, 4953
BstX2I	RGATCY	5 cut(s)	2392, 3514, 4284, 4374, 4673
BstXI	CCANNNNNNTGG	2 cut(s)	1591, 2201
BstYI	RGATCY	5 cut(s)	2392, 3514, 4284, 4374, 4673
BstZ17I	GTATAC	1 cut(s)	5019
BstZI	CGGCCG	1 cut(s)	415
Bsu15I	ATCGAT	1 cut(s)	3300
BsuI	GTATCC	1 cut(s)	3904
BsuTUI	ATCGAT	1 cut(s)	3300
BtgI	CCRYGG	2 cut(s)	3824, 4426
BtrI	CACGTC	2 cut(s)	3541, 4037
BtsCI	GGATG	8 cut(s)	2530, 2821, 2943, 3490, 4123, 4422, 4444, 4906
BtsI	GCAGTG	3 cut(s)	4002, 4616, 4976
BtsIMutI	CAGTG	7 cut(s)	1338, 3165, 4002, 4195, 4616, 4745, 4976
BveI	ACCTGC	3 cut(s)	814, 4763, 5004
Cac8I	GCNNGC	6 cut(s)	632, 758, 3356, 3845, 4078, 4137
CaiI	CAGNNNCTG	2 cut(s)	2614, 4385
CciI	TCATGA	2 cut(s)	328, 3729
CfoI	GCGC	3 cut(s)	21, 283, 2656
Cfr10I	RCCGGY	4 cut(s)	321, 393, 3011, 4560
Cfr13I	GGNCC	5 cut(s)	210, 319, 391, 4346, 5082
ClaI	ATCGAT	1 cut(s)	3300
CseI	GACGC	3 cut(s)	191, 1864, 2971
CsiI	ACCWGGT	1 cut(s)	4886
CspAI	ACCGGT	1 cut(s)	321
DraI	TTTAAA	1 cut(s)	1897
DraIII	CACNNNGTG	1 cut(s)	1490
EaeI	YGGCCR	3 cut(s)	415, 1735, 5145
EagI	CGGCCG	1 cut(s)	415
Eam1104I	CTCTTC	4 cut(s)	4038, 4452, 4813, 4991
EarI	CTCTTC	4 cut(s)	4038, 4452, 4813, 4991
EciI	GGCGGA	3 cut(s)	607, 674, 3600
Ecl136II	GAGCTC	1 cut(s)	385
EclXI	CGGCCG	1 cut(s)	415
Eco130I	CCWWGG	3 cut(s)	2916, 3824, 4426
Eco24I	GRGCYC	2 cut(s)	387, 4365
Eco32I	GATATC	2 cut(s)	3400, 3996
Eco47I	GGWCC	2 cut(s)	319, 391
Eco52I	CGGCCG	1 cut(s)	415
Eco53kI	GAGCTC	1 cut(s)	385
Eco57I	CTGAAG	8 cut(s)	1362, 1941, 2690, 3108, 3243, 3299, 4194, 4718
Eco72I	CACGTG	2 cut(s)	1490, 2399
Eco88I	CYCGRG	3 cut(s)	2253, 4313, 4565
Eco91I	GGTNACC	1 cut(s)	2204
EcoICRI	GAGCTC	1 cut(s)	385
EcoO65I	GGTNACC	1 cut(s)	2204
EcoRI	GAATTC	1 cut(s)	51
EcoRV	GATATC	2 cut(s)	3400, 3996
EcoT14I	CCWWGG	3 cut(s)	2916, 3824, 4426
EcoT38I	GRGCYC	2 cut(s)	387, 4365
ErhI	CCWWGG	3 cut(s)	2916, 3824, 4426
Esp3I	CGTCTC	3 cut(s)	364, 2250, 4800
FalI	AAGNNNNNCTT	4 cut(s)	1179, 1211, 2145, 2177
FaqI	GGGAC	6 cut(s)	295, 404, 1435, 2776, 3491, 4849
FauI	CCCGC	7 cut(s)	24, 65, 90, 128, 2129, 3331, 3883
FauNDI	CATATG	1 cut(s)	4532
FbaI	TGATCA	3 cut(s)	1704, 2482, 3894
FblI	GTMKAC	2 cut(s)	1201, 5018
FokI	GGATG	8 cut(s)	2517, 2828, 2950, 3497, 4130, 4409, 4451, 4893
FriOI	GRGCYC	2 cut(s)	387, 4365
FspBI	CTAG	6 cut(s)	284, 1209, 1353, 2549, 2568, 4113
GlaI	GCGC	3 cut(s)	20, 282, 2655
GsaI	CCCAGC	4 cut(s)	823, 2281, 3165, 5083
GsuI	CTGGAG	5 cut(s)	216, 3053, 4071, 4127, 4745
HgaI	GACGC	3 cut(s)	191, 1864, 2971
HhaI	GCGC	3 cut(s)	21, 283, 2656
Hin1I	GRCGYC	4 cut(s)	183, 1875, 2982, 4484
Hin6I	GCGC	3 cut(s)	19, 281, 2654
HinP1I	GCGC	3 cut(s)	19, 281, 2654
HincII	GTYRAC	5 cut(s)	1202, 1995, 2721, 3184, 4336
HindII	GTYRAC	5 cut(s)	1202, 1995, 2721, 3184, 4336
HindIII	AAGCTT	3 cut(s)	127, 2159, 2702
HpaI	GTTAAC	2 cut(s)	1995, 4336
Hpy99I	CGWCG	6 cut(s)	49, 140, 185, 383, 1206, 5114
HpyCH4III	ACNGT	9 cut(s)	77, 832, 1206, 2326, 2740, 2771, 2975, 4574, 4844
HpyCH4IV	ACGT	6 cut(s)	1489, 2398, 3540, 4036, 4180, 4484
HpySE526I	ACGT	6 cut(s)	1489, 2398, 3540, 4036, 4180, 4484
Hsp92I	GRCGYC	4 cut(s)	183, 1875, 2982, 4484
HspAI	GCGC	3 cut(s)	19, 281, 2654
KpnI	GGTACC	1 cut(s)	2382
Ksp22I	TGATCA	3 cut(s)	1704, 2482, 3894
KspAI	GTTAAC	2 cut(s)	1995, 4336
LguI	GCTCTTC	3 cut(s)	4038, 4813, 4991
MabI	ACCWGGT	1 cut(s)	4886
MaeI	CTAG	6 cut(s)	284, 1209, 1353, 2549, 2568, 4113
MaeII	ACGT	6 cut(s)	1489, 2398, 3540, 4036, 4180, 4484
MbiI	CCGCTC	1 cut(s)	4593
MfeI	CAATTG	3 cut(s)	584, 1494, 5022
MflI	RGATCY	5 cut(s)	2392, 3514, 4284, 4374, 4673
MhlI	GDGCHC	3 cut(s)	387, 4365, 4660
MlsI	TGGCCA	1 cut(s)	5147
MluNI	TGGCCA	1 cut(s)	5147
MlyI	GAGTC	5 cut(s)	169, 317, 730, 2348, 4625
MmeI	TCCRAC	2 cut(s)	1431, 4312
Mox20I	TGGCCA	1 cut(s)	5147
MroXI	GAANNNNTTC	2 cut(s)	689, 1519
MscI	TGGCCA	1 cut(s)	5147
MslI	CAYNNNNRTG	6 cut(s)	1842, 2180, 2199, 2327, 3393, 4535
Msp20I	TGGCCA	1 cut(s)	5147
MspA1I	CMGCKG	8 cut(s)	871, 1241, 2105, 2710, 3242, 3326, 3907, 4091
MunI	CAATTG	3 cut(s)	584, 1494, 5022
Mva1269I	GAATGC	5 cut(s)	755, 2954, 3956, 3973, 5120
MvnI	CGCG	2 cut(s)	19, 1311
NciI	CCSGG	5 cut(s)	31, 313, 389, 1671, 4934
NcoI	CCATGG	2 cut(s)	3824, 4426
NdeI	CATATG	1 cut(s)	4532
NmeAIII	GCCGAG	3 cut(s)	931, 2017, 4900
NmuCI	GTSAC	6 cut(s)	826, 1054, 2066, 2204, 2414, 2623
NspI	RCATGY	4 cut(s)	2522, 4869, 4884, 4994
NspV	TTCGAA	1 cut(s)	1049
PaeR7I	CTCGAG	2 cut(s)	2253, 4565
PagI	TCATGA	2 cut(s)	328, 3729
PasI	CCCWGGG	1 cut(s)	4379
PciI	ACATGT	2 cut(s)	4880, 4990
PciSI	GCTCTTC	3 cut(s)	4038, 4813, 4991
PctI	GAATGC	5 cut(s)	755, 2954, 3956, 3973, 5120
PdmI	GAANNNNTTC	2 cut(s)	689, 1519
PfoI	TCCNGGA	1 cut(s)	387
PinAI	ACCGGT	1 cut(s)	321
PleI	GAGTC	5 cut(s)	168, 316, 729, 2347, 4624
PmaCI	CACGTG	2 cut(s)	1490, 2399
PmlI	CACGTG	2 cut(s)	1490, 2399
PpsI	GAGTC	5 cut(s)	168, 316, 729, 2347, 4624
Ppu21I	YACGTR	2 cut(s)	1490, 2399
PscI	ACATGT	2 cut(s)	4880, 4990
PshBI	ATTAAT	1 cut(s)	2051
PsiI	TTATAA	1 cut(s)	1226
Psp124BI	GAGCTC	1 cut(s)	387
PspCI	CACGTG	2 cut(s)	1490, 2399
PspEI	GGTNACC	1 cut(s)	2204
PspFI	CCCAGC	4 cut(s)	819, 2277, 3161, 5079
PspPI	GGNCC	5 cut(s)	210, 319, 391, 4346, 5082
PspXI	VCTCGAGB	1 cut(s)	4565
PsrI	GAACNNNNNNTAC	2 cut(s)	4922, 4954
PstI	CTGCAG	2 cut(s)	1086, 3692
PstNI	CAGNNNCTG	2 cut(s)	2614, 4385
PsuI	RGATCY	5 cut(s)	2392, 3514, 4284, 4374, 4673
PvuII	CAGCTG	7 cut(s)	871, 1241, 2105, 2710, 3242, 3907, 4091
RseI	CAYNNNNRTG	6 cut(s)	1842, 2180, 2199, 2327, 3393, 4535
SacI	GAGCTC	1 cut(s)	387
SalI	GTCGAC	1 cut(s)	1200
SapI	GCTCTTC	3 cut(s)	4038, 4813, 4991
Sau96I	GGNCC	5 cut(s)	210, 319, 391, 4346, 5082
SchI	GAGTC	5 cut(s)	169, 317, 730, 2348, 4625
SduI	GDGCHC	3 cut(s)	387, 4365, 4660
SexAI	ACCWGGT	1 cut(s)	4886
SfcI	CTRYAG	7 cut(s)	803, 1082, 2994, 3600, 3688, 4065, 4780
Sfr274I	CTCGAG	2 cut(s)	2253, 4565
SfuI	TTCGAA	1 cut(s)	1049
SinI	GGWCC	2 cut(s)	319, 391
SlaI	CTCGAG	2 cut(s)	2253, 4565
SmiMI	CAYNNNNRTG	6 cut(s)	1842, 2180, 2199, 2327, 3393, 4535
SmlI	CTYRAG	6 cut(s)	644, 2253, 3116, 3922, 4280, 4565
SmoI	CTYRAG	6 cut(s)	644, 2253, 3116, 3922, 4280, 4565
SspI	AATATT	2 cut(s)	1265, 3312
SspMI	CTAG	6 cut(s)	284, 1209, 1353, 2549, 2568, 4113
SstI	GAGCTC	1 cut(s)	387
StyI	CCWWGG	3 cut(s)	2916, 3824, 4426
TaaI	ACNGT	9 cut(s)	77, 832, 1206, 2326, 2740, 2771, 2975, 4574, 4844
TaiI	ACGT	6 cut(s)	1492, 2401, 3543, 4039, 4183, 4487
TatI	WGTACW	5 cut(s)	863, 1399, 2229, 4192, 4477
TauI	GCSGC	5 cut(s)	520, 756, 3230, 3776, 4593
TscAI	CASTG	7 cut(s)	1345, 3172, 4009, 4195, 4616, 4752, 4976
TseFI	GTSAC	6 cut(s)	826, 1054, 2066, 2204, 2414, 2623
Tsp45I	GTSAC	6 cut(s)	826, 1054, 2066, 2204, 2414, 2623
TspGWI	ACGGA	2 cut(s)	453, 1008
TspRI	CASTG	7 cut(s)	1345, 3172, 4009, 4195, 4616, 4752, 4976
VneI	GTGCAC	1 cut(s)	4656
VpaK11BI	GGWCC	2 cut(s)	319, 391
VspI	ATTAAT	1 cut(s)	2051
XceI	RCATGY	4 cut(s)	2522, 4869, 4884, 4994
XcmI	CCANNNNNNNNNTGG	2 cut(s)	244, 1556
XhoI	CTCGAG	2 cut(s)	2253, 4565
XmiI	GTMKAC	2 cut(s)	1201, 5018
XmnI	GAANNNNTTC	2 cut(s)	689, 1519
XspI	CTAG	6 cut(s)	284, 1209, 1353, 2549, 2568, 4113
ZraI	GACGTC	1 cut(s)	4485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.