pycom15g30040

Egg cell-secreted protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008373
Physical Location & Seq
Forward (+)
27313526 .. 27313975
450 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 450 bp
ATGGCTTTACAAAATGTAGCTCTCCTGGTGACAGTAAGCCTAATATTCACCGGTGCAAACTATGCACTTGCAGCAAGGAACGTGATCGATCCCATTCAGCCAGCCTACAGCATCCCCATCACTGCGAGAATCAAAACTGGAAGTGAAGGAAATGGCGGGCTAGTCGATTGCTGGAATGCGCTTGCGGAGCTGAAATTATGCTCCAATGAGGTGGTTCTGTTTTTCCTGAATGGGCAGGCTGATATAGGGCCTGATTGTTGCCAAGCCATTGCCACTATTACGCGCCACTGCTGGCCTGCAATGCTGACTTCCATCGGTTTCACGGCTGAGGAGGGTAACATTTTGCGAGGCTACTGTGACGCCGCTGCATCTGCTACTACTACTAATTCCTCCGCCCCTGCTTCATCTCCTCTTGCTGCCGCCACCACGCCTACTCCTCCCGCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

150

Amino Acids

15.37

Weight (kDa)

4.64

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 56 - 120 4.7e-20 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 283
AciI CCGC 6 cut(s) 156, 185, 363, 393, 420, 441
AclWI GGATC 1 cut(s) 83
AcyI GRCGYC 1 cut(s) 360
AgeI ACCGGT 1 cut(s) 50
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 2 cut(s) 20, 190
AluI AGCT 2 cut(s) 20, 190
AlwI GGATC 1 cut(s) 83
AoxI GGCC 2 cut(s) 248, 293
ApeKI GCWGC 3 cut(s) 71, 365, 416
AsiGI ACCGGT 1 cut(s) 50
AspLEI GCGC 2 cut(s) 181, 285
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 40, 40
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 3 cut(s) 83, 352, 403
BccI CCATC 2 cut(s) 125, 320
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 1 cut(s) 26
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 106
BisI GCNGC 5 cut(s) 72, 363, 366, 417, 420
BlsI GCNGC 5 cut(s) 73, 364, 367, 418, 421
Bme1390I CCNGG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 26
BmsI GCATC 2 cut(s) 120, 377
Bpu10I CCTNAGC 1 cut(s) 327
Bsa29I ATCGAT 1 cut(s) 87
BsaHI GRCGYC 1 cut(s) 360
BsaWI WCCGGW 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 142
Bse3DI GCAATG 2 cut(s) 267, 306
BseBI CCWGG 1 cut(s) 26
BseCI ATCGAT 1 cut(s) 87
BseGI GGATG 1 cut(s) 111
BseMI GCAATG 2 cut(s) 267, 306
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 1 cut(s) 142
BseRI GAGGAG 3 cut(s) 344, 399, 426
BseXI GCAGC 3 cut(s) 83, 352, 403
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 2 cut(s) 250, 295
BshTI ACCGGT 1 cut(s) 50
BshVI ATCGAT 1 cut(s) 87
BsiSI CCGG 1 cut(s) 51
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 2 cut(s) 250, 295
Bsp143I GATC 2 cut(s) 84, 88
BspACI CCGC 6 cut(s) 156, 185, 363, 393, 420, 441
BspANI GGCC 2 cut(s) 250, 295
BspCNI CTCAG 1 cut(s) 319
BspDI ATCGAT 1 cut(s) 87
BspFNI CGCG 1 cut(s) 283
BspPI GGATC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 267, 306
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 50
BssMI GATC 2 cut(s) 84, 88
BssNI GRCGYC 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 2 cut(s) 34, 356
BstACI GRCGYC 1 cut(s) 360
BstAPI GCANNNNNTGC 1 cut(s) 62
BstC8I GCNNGC 6 cut(s) 102, 158, 183, 237, 293, 297
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 2 cut(s) 181, 285
BstKTI GATC 2 cut(s) 87, 91
BstMBI GATC 2 cut(s) 84, 88
BstMWI GCNNNNNNNGC 5 cut(s) 62, 71, 187, 301, 371
BstNI CCWGG 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 24
BstSFI CTRYAG 1 cut(s) 106
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 3 cut(s) 83, 352, 403
BstXI CCANNNNNNTGG 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 87
BsuRI GGCC 2 cut(s) 250, 295
BsuTUI ATCGAT 1 cut(s) 87
BtsCI GGATG 1 cut(s) 111
BtsI GCAGTG 2 cut(s) 120, 286
BtsIMutI CAGTG 2 cut(s) 120, 286
Cac8I GCNNGC 6 cut(s) 102, 158, 183, 237, 293, 297
CfoI GCGC 2 cut(s) 181, 285
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 248
ClaI ATCGAT 1 cut(s) 87
CseI GACGC 1 cut(s) 368
CspAI ACCGGT 1 cut(s) 50
DdeI CTNAG 1 cut(s) 327
DpnI GATC 2 cut(s) 86, 90
DpnII GATC 2 cut(s) 84, 88
EciI GGCGGA 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 24
FaiI YATR 3 cut(s) 63, 199, 245
FauI CCCGC 2 cut(s) 149, 448
Fnu4HI GCNGC 5 cut(s) 72, 363, 366, 417, 420
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 5 cut(s) 72, 363, 366, 417, 420
FspBI CTAG 1 cut(s) 161
GlaI GCGC 2 cut(s) 180, 284
GluI GCNGC 5 cut(s) 72, 363, 366, 417, 420
HaeIII GGCC 2 cut(s) 250, 295
HapII CCGG 1 cut(s) 51
HgaI GACGC 1 cut(s) 368
HhaI GCGC 2 cut(s) 181, 285
Hin1I GRCGYC 1 cut(s) 360
Hin6I GCGC 2 cut(s) 179, 283
HinP1I GCGC 2 cut(s) 179, 283
HinfI GANTC 1 cut(s) 129
HpaII CCGG 1 cut(s) 51
HphI GGTGA 2 cut(s) 40, 40
Hpy188III TCNNGA 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 2 cut(s) 34, 356
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 5 cut(s) 56, 65, 71, 299, 368
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 71, 187, 301, 371
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 360
HspAI GCGC 2 cut(s) 179, 283
Kzo9I GATC 2 cut(s) 84, 88
LmnI GCTCC 2 cut(s) 187, 206
Lsp1109I GCAGC 3 cut(s) 83, 352, 403
LweI GCATC 2 cut(s) 120, 377
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 3 cut(s) 28, 335, 356
MalI GATC 2 cut(s) 86, 90
MboI GATC 2 cut(s) 84, 88
MluCI AATT 2 cut(s) 194, 385
MnlI CCTC 7 cut(s) 202, 322, 325, 341, 400, 420, 447
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 181
MvaI CCWGG 1 cut(s) 26
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 5 cut(s) 62, 71, 187, 301, 371
NdeII GATC 2 cut(s) 84, 88
NmuCI GTSAC 2 cut(s) 28, 356
PctI GAATGC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 129
PinAI ACCGGT 1 cut(s) 50
PkrI GCNGC 5 cut(s) 73, 364, 367, 418, 421
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspPI GGNCC 1 cut(s) 248
SatI GCNGC 5 cut(s) 72, 363, 366, 417, 420
Sau3AI GATC 2 cut(s) 84, 88
Sau96I GGNCC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 26
SetI ASST 4 cut(s) 22, 84, 192, 213
SfaNI GCATC 2 cut(s) 120, 377
SfcI CTRYAG 1 cut(s) 106
SgrAI CRCCGGYG 1 cut(s) 50
Sse9I AATT 2 cut(s) 194, 385
SsiI CCGC 6 cut(s) 156, 185, 363, 393, 420, 441
SspI AATATT 1 cut(s) 45
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 1 cut(s) 24
TaaI ACNGT 2 cut(s) 34, 356
TaiI ACGT 1 cut(s) 84
TaqI TCGA 2 cut(s) 87, 165
TasI AATT 2 cut(s) 194, 385
TauI GCSGC 2 cut(s) 365, 422
TfiI GAWTC 1 cut(s) 129
TscAI CASTG 2 cut(s) 127, 293
TseFI GTSAC 2 cut(s) 28, 356
TseI GCWGC 3 cut(s) 71, 365, 416
Tsp45I GTSAC 2 cut(s) 28, 356
TspDTI ATGAA 1 cut(s) 393
TspRI CASTG 2 cut(s) 127, 293
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.