pycom16g01020

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003764
Physical Location & Seq
Reverse (-)
624266 .. 625057
792 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 588 bp
ATGAGGCCGATCCTGATGAAGGGGCACGAGAGGCCCTTGACTTTCCTCAAATACAACAGGGACGGCGACCTCCTCTTCTCCTGCGCCAAGGACCACAACCCCACCGTCTGGTTCGCCGACAACGGCGAGCGCCTCGGTACCTACCGCGGACACAACGGCGCCGTCTGGTGCTGCGATGTGTCTAGAGACTCCGCCCGCCTCATTACCGGCAGCGCCGATCAGACCGCCAAGCTCTGGAACGTTCAGACCGGTCAGCAGCTCTTCACCTTCAATTTTGACTCGCCGGCGAGGGCTGTCGACTTCTCCGTCGGCGATAAACTCGCCGTCATCACCACCGACCCCTTCATGGACGTTACGTCGGCGATTCACGTCAAGCGCATCGCCAGTGACCCCGAAGATCAGGTTGGGGAATCGGCTCTGATACTCAAGGGACCTCAGGGAAGAATAAACAGAGCTGTTTGGGGCCCTCTGAACAGAACCATCATAAGCGCTGGAGAAGACGCAGTTATTCGCATTTGGGATTCTGAGACTGGTAAGCTACTTAAGGAGAATGATCGATCTCGGAAGTGGGTCACAAAAAGACCATAA

Protein Analysis

196

Amino Acids

21.84

Weight (kDa)

8.82

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 1 - 191 3.9e-63 EIF3I
Beta-prop_WDR3_1st PF25173 5 - 90 3.4e-16 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 6 - 107 1.2e-16 WDR5 beta-propeller domain
WD40_Prp19 PF24814 6 - 92 4.1e-08 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 6 - 173 3e-13 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 6 - 173 2.9e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 7 - 80 3.6e-10 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 109 2.6e-07 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 11 - 183 1e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 12 - 126 3.1e-18 THOC3 beta-propeller domain
WD40_Gbeta PF25391 14 - 80 1e-07 G protein beta WD-40 repeat protein
WD40 PF00400 42 - 80 3.6e-10 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 57 - 175 1.3e-06 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
Acc65I GGTACC 1 cut(s) 137
AccB1I GGYRCC 2 cut(s) 137, 158
AccB7I CCANNNNNTGG 2 cut(s) 108, 234
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 147
AciI CCGC 5 cut(s) 145, 147, 192, 196, 225
AclI AACGTT 1 cut(s) 240
AclWI GGATC 1 cut(s) 4
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 139
AfeI AGCGCT 1 cut(s) 490
AfiI CCNNNNNNNGG 4 cut(s) 19, 108, 234, 346
AflII CTTAAG 1 cut(s) 542
AgeI ACCGGT 1 cut(s) 248
AgsI TTSAA 1 cut(s) 271
AhdI GACNNNNNGTC 1 cut(s) 355
AjiI CACGTC 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 4 cut(s) 232, 259, 455, 538
AluI AGCT 4 cut(s) 232, 259, 455, 538
Alw26I GTCTC 2 cut(s) 180, 521
AlwI GGATC 1 cut(s) 4
Aor51HI AGCGCT 1 cut(s) 490
AoxI GGCC 3 cut(s) 5, 32, 463
ApaI GGGCCC 1 cut(s) 467
ApeKI GCWGC 3 cut(s) 171, 210, 256
AsiGI ACCGGT 1 cut(s) 248
Asp718I GGTACC 1 cut(s) 137
AspLEI GCGC 6 cut(s) 86, 132, 161, 215, 378, 491
AspS9I GGNCC 5 cut(s) 33, 91, 431, 463, 464
AsuHPI GGTGA 2 cut(s) 256, 322
AvaII GGWCC 2 cut(s) 91, 431
AxyI CCTNAGG 1 cut(s) 435
BaeGI GKGCMC 2 cut(s) 27, 467
BanI GGYRCC 2 cut(s) 137, 158
BanII GRGCYC 1 cut(s) 467
BauI CACGAG 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 504
BbvI GCAGC 3 cut(s) 158, 222, 268
BccI CCATC 1 cut(s) 488
BceAI ACGGC 5 cut(s) 79, 139, 146, 172, 308
BcoDI GTCTC 2 cut(s) 180, 521
BfaI CTAG 1 cut(s) 183
BfoI RGCGCY 4 cut(s) 133, 162, 216, 492
BfrI CTTAAG 1 cut(s) 542
BisI GCNGC 3 cut(s) 172, 211, 257
BlsI GCNGC 3 cut(s) 173, 212, 258
Bme18I GGWCC 2 cut(s) 91, 431
BmeRI GACNNNNNGTC 1 cut(s) 355
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 5 cut(s) 33, 91, 431, 463, 464
BmiI GGNNCC 5 cut(s) 139, 160, 432, 464, 465
BmsI GCATC 1 cut(s) 387
BpiI GAAGAC 1 cut(s) 504
BpmI CTGGAG 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 410
Bsa29I ATCGAT 1 cut(s) 556
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 87, 133, 145
BsaWI WCCGGW 1 cut(s) 248
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 108, 234, 346
Bse118I RCCGGY 3 cut(s) 206, 248, 283
Bse1I ACTGG 2 cut(s) 384, 535
Bse21I CCTNAGG 1 cut(s) 435
BseCI ATCGAT 1 cut(s) 556
BseDI CCNNGG 3 cut(s) 87, 133, 145
BseLI CCNNNNNNNGG 4 cut(s) 19, 108, 234, 346
BseMII CTCAG 2 cut(s) 449, 516
BseNI ACTGG 2 cut(s) 384, 535
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 2 cut(s) 27, 467
BseXI GCAGC 3 cut(s) 158, 222, 268
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 3 cut(s) 7, 34, 465
BshNI GGYRCC 2 cut(s) 137, 158
BshTI ACCGGT 1 cut(s) 248
BshVI ATCGAT 1 cut(s) 556
BsiSI CCGG 3 cut(s) 207, 249, 284
BslFI GGGAC 2 cut(s) 74, 444
BslI CCNNNNNNNGG 4 cut(s) 19, 108, 234, 346
BsmAI GTCTC 2 cut(s) 180, 521
BsmFI GGGAC 2 cut(s) 74, 444
BsnI GGCC 3 cut(s) 7, 34, 465
Bsp120I GGGCCC 1 cut(s) 463
Bsp1286I GDGCHC 2 cut(s) 27, 467
Bsp143I GATC 5 cut(s) 9, 217, 397, 553, 557
BspACI CCGC 5 cut(s) 145, 147, 192, 196, 225
BspANI GGCC 3 cut(s) 7, 34, 465
BspCNI CTCAG 2 cut(s) 448, 517
BspDI ATCGAT 1 cut(s) 556
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 5 cut(s) 139, 160, 432, 464, 465
BspPI GGATC 1 cut(s) 4
BspQI GCTCTTC 1 cut(s) 266
BspT107I GGYRCC 2 cut(s) 137, 158
BspTI CTTAAG 1 cut(s) 542
BsrFI RCCGGY 3 cut(s) 206, 248, 283
BsrI ACTGG 2 cut(s) 384, 535
BssAI RCCGGY 3 cut(s) 206, 248, 283
BssECI CCNNGG 3 cut(s) 87, 133, 145
BssMI GATC 5 cut(s) 9, 217, 397, 553, 557
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 2 cut(s) 80, 266
BstACI GRCGYC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 542
BstC8I GCNNGC 3 cut(s) 128, 196, 285
BstDEI CTNAG 2 cut(s) 435, 525
BstDSI CCRYGG 1 cut(s) 145
BstENI CCTNNNNNAGG 1 cut(s) 17
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 4 cut(s) 133, 162, 216, 492
BstHHI GCGC 6 cut(s) 86, 132, 161, 215, 378, 491
BstKTI GATC 5 cut(s) 12, 220, 400, 556, 560
BstMAI GTCTC 2 cut(s) 180, 521
BstMBI GATC 5 cut(s) 9, 217, 397, 553, 557
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstSLI GKGCMC 2 cut(s) 27, 467
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 158, 222, 268
BstV2I GAAGAC 1 cut(s) 504
Bsu15I ATCGAT 1 cut(s) 556
Bsu36I CCTNAGG 1 cut(s) 435
BsuRI GGCC 3 cut(s) 7, 34, 465
BsuTUI ATCGAT 1 cut(s) 556
BtgI CCRYGG 1 cut(s) 145
BtgZI GCGATG 2 cut(s) 189, 364
BtrI CACGTC 1 cut(s) 370
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 3 cut(s) 128, 196, 285
CfoI GCGC 6 cut(s) 86, 132, 161, 215, 378, 491
Cfr10I RCCGGY 3 cut(s) 206, 248, 283
Cfr13I GGNCC 5 cut(s) 33, 91, 431, 463, 464
Cfr42I CCGCGG 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 556
CseI GACGC 1 cut(s) 509
Csp6I GTAC 1 cut(s) 138
CspAI ACCGGT 1 cut(s) 248
CviAII CATG 1 cut(s) 346
CviJI RGCY 9 cut(s) 7, 34, 232, 259, 293, 416, 455, 465, 538
CviKI_1 RGCY 9 cut(s) 7, 34, 232, 259, 293, 416, 455, 465, 538
CviQI GTAC 1 cut(s) 138
DdeI CTNAG 2 cut(s) 435, 525
DinI GGCGCC 1 cut(s) 160
DpnI GATC 5 cut(s) 11, 219, 399, 555, 559
DpnII GATC 5 cut(s) 9, 217, 397, 553, 557
DrdI GACNNNNNNGTC 1 cut(s) 305
DriI GACNNNNNGTC 1 cut(s) 355
DseDI GACNNNNNNGTC 1 cut(s) 305
Eam1104I CTCTTC 2 cut(s) 80, 266
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 2 cut(s) 80, 266
EciI GGCGGA 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 467
Eco47I GGWCC 2 cut(s) 91, 431
Eco47III AGCGCT 1 cut(s) 490
Eco81I CCTNAGG 1 cut(s) 435
EcoNI CCTNNNNNAGG 1 cut(s) 17
EcoO109I RGGNCCY 4 cut(s) 33, 431, 463, 464
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 467
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 1 cut(s) 349
FaiI YATR 3 cut(s) 347, 485, 586
FaqI GGGAC 2 cut(s) 74, 444
FatI CATG 1 cut(s) 345
FauI CCCGC 1 cut(s) 203
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 3 cut(s) 172, 211, 257
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 3 cut(s) 172, 211, 257
FspBI CTAG 1 cut(s) 183
GlaI GCGC 6 cut(s) 85, 131, 160, 214, 377, 490
GluI GCNGC 3 cut(s) 172, 211, 257
GsuI CTGGAG 1 cut(s) 513
HaeII RGCGCY 4 cut(s) 133, 162, 216, 492
HaeIII GGCC 3 cut(s) 7, 34, 465
HapII CCGG 3 cut(s) 207, 249, 284
HgaI GACGC 1 cut(s) 509
HhaI GCGC 6 cut(s) 86, 132, 161, 215, 378, 491
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 1 cut(s) 349
Hin6I GCGC 6 cut(s) 84, 130, 159, 213, 376, 489
HinP1I GCGC 6 cut(s) 84, 130, 159, 213, 376, 489
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 5 cut(s) 188, 278, 364, 410, 521
HpaII CCGG 3 cut(s) 207, 249, 284
HphI GGTGA 2 cut(s) 256, 322
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 6 cut(s) 222, 246, 420, 471, 526, 564
Hpy188III TCNNGA 3 cut(s) 13, 183, 235
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 2 cut(s) 311, 361
HpyAV CCTTC 3 cut(s) 13, 277, 352
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 4 cut(s) 240, 351, 356, 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 2 cut(s) 435, 525
HpySE526I ACGT 4 cut(s) 240, 351, 356, 369
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 1 cut(s) 349
HspAI GCGC 6 cut(s) 84, 130, 159, 213, 376, 489
KasI GGCGCC 1 cut(s) 158
KpnI GGTACC 1 cut(s) 141
KroI GCCGGC 1 cut(s) 283
KroNI GCCGGC 1 cut(s) 285
KspI CCGCGG 1 cut(s) 148
Kzo9I GATC 5 cut(s) 9, 217, 397, 553, 557
LguI GCTCTTC 1 cut(s) 266
Lsp1109I GCAGC 3 cut(s) 158, 222, 268
LweI GCATC 1 cut(s) 387
MaeI CTAG 1 cut(s) 183
MaeII ACGT 4 cut(s) 240, 351, 356, 369
MaeIII GTNAC 3 cut(s) 352, 386, 571
MalI GATC 5 cut(s) 11, 219, 399, 555, 559
MboI GATC 5 cut(s) 9, 217, 397, 553, 557
MboII GAAGA 5 cut(s) 67, 253, 407, 453, 509
MhlI GDGCHC 2 cut(s) 27, 467
MluCI AATT 1 cut(s) 271
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 2 cut(s) 182, 272
MnlI CCTC 9 cut(s) 24, 56, 80, 83, 143, 209, 282, 444, 477
MreI CGCCGGCG 1 cut(s) 283
MroNI GCCGGC 1 cut(s) 283
MseI TTAA 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 147
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 3 cut(s) 207, 249, 284
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 31
NaeI GCCGGC 1 cut(s) 285
NarI GGCGCC 1 cut(s) 159
NdeII GATC 5 cut(s) 9, 217, 397, 553, 557
NgoMIV GCCGGC 1 cut(s) 283
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 5 cut(s) 139, 160, 432, 464, 465
NmuCI GTSAC 2 cut(s) 386, 571
PciSI GCTCTTC 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 285
PfeI GAWTC 3 cut(s) 364, 410, 521
PflMI CCANNNNNTGG 2 cut(s) 108, 234
PinAI ACCGGT 1 cut(s) 248
PkrI GCNGC 3 cut(s) 173, 212, 258
PleI GAGTC 2 cut(s) 182, 272
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 2 cut(s) 182, 272
PpuMI RGGWCCY 1 cut(s) 431
Psp1406I AACGTT 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 431
PspN4I GGNNCC 5 cut(s) 139, 160, 432, 464, 465
PspOMI GGGCCC 1 cut(s) 463
PspPI GGNCC 5 cut(s) 33, 91, 431, 463, 464
PspPPI RGGWCCY 1 cut(s) 431
RsaI GTAC 1 cut(s) 139
RsaNI GTAC 1 cut(s) 138
SacII CCGCGG 1 cut(s) 148
SalI GTCGAC 1 cut(s) 296
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 3 cut(s) 172, 211, 257
Sau3AI GATC 5 cut(s) 9, 217, 397, 553, 557
Sau96I GGNCC 5 cut(s) 33, 91, 431, 463, 464
SchI GAGTC 2 cut(s) 182, 272
SduI GDGCHC 2 cut(s) 27, 467
SfaNI GCATC 1 cut(s) 387
SfoI GGCGCC 1 cut(s) 160
Sfr303I CCGCGG 1 cut(s) 148
SgrAI CRCCGGYG 1 cut(s) 283
SgrBI CCGCGG 1 cut(s) 148
SinI GGWCC 2 cut(s) 91, 431
SmlI CTYRAG 2 cut(s) 425, 542
SmoI CTYRAG 2 cut(s) 425, 542
Sse9I AATT 1 cut(s) 271
SsiI CCGC 5 cut(s) 145, 147, 192, 196, 225
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 4 cut(s) 243, 354, 359, 372
TaqI TCGA 2 cut(s) 297, 556
TasI AATT 1 cut(s) 271
TfiI GAWTC 3 cut(s) 364, 410, 521
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 1 cut(s) 391
TseFI GTSAC 2 cut(s) 386, 571
TseI GCWGC 3 cut(s) 171, 210, 256
Tsp45I GTSAC 2 cut(s) 386, 571
TspDTI ATGAA 2 cut(s) 32, 334
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 1 cut(s) 391
Van91I CCANNNNNTGG 2 cut(s) 108, 234
Vha464I CTTAAG 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 91, 431
XagI CCTNNNNNAGG 1 cut(s) 17
XbaI TCTAGA 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 297
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.