Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Reverse (-)

973023 .. 977299

Gene Structure 4277 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3351 bp

ATGGGTAAGACGATACAGTTGTTTGGATTCCCTTCTGGTGCATCTGCAGAAGCTGTTTTTGAATGGCTGGAGAGAAAAACTGGAAAAGGATCTGTTTATGCTATGAGATACAGACAAGCCAAAAATTCAAGGAGATATTATGCTATTATTCAGTTTGAAACCGCCAATGATGCTGAGTTTATTAATTCCTTGGCCAACACTGGAAACCTGTGGTATGGGAAAAGCTATCTGAAGTCCCGGAAGATGGATCATGATATTGTACCAAAGCCAAGAACCGCTTTGCACAGTCTGCAAAATCTAGGATTGTATTTTGGTTGCAAGACCGGAAAAGAAGAGCTATCTTATTTTTGGTGTTCGGACAATGTTTCAGTGGACTTTGGGATTAGAATACGGAAATTAATTTGTCTTCTGAAATATGATGAGGTGGAATATAAGCTTGAACTTTCCTATGAGAACATTTGGCAGACTGAGTTGTATAGTCCACGTGGTCACACTGCTAAGAAACTTCTGTTTCAGTTATTGGGAGCGCCTCGGATTTTCGAGAAAGAAGCAGACTCTTCAGGACAAGCATATGAAGACCCTTTTCAGAACTATTGCAGGGAAACCCCTGATGACCAATGGATCCGGACAACTGATTTCACTCCATCATGCGCTATTGGGCAGTCTTCTGCATTATGCTTGGAGCTTCCTTATGGTCTCCAACTTCCAGATTTTGGAGAAAACTTTCCCGGGTACAAGGGGATTTTAAGGGACCTTGTCACGGAATTATGTCCCCCCTTTTCTTGCTCGGATCTGGGAGCCATGGTTTGTCCTCCTGAATGTGTTGGTGTTCCATATGATATCTTGTTTAAGGTAAACTCCCTGGTTCAGCAAGGGTGGCTTCCTGGGCCAAAACTTGGTGATAATTTTTTTGAGTTGGTTGATGCACGCAGAAGAGACCTTGCATGCATAGAGTATGCTCTAGATAAATTATACAATTTAAGAGATAGCTGTTATGAACCTTCAATGTGGCTTGAGGAGCAGTATATGAAGTACCGCAGATTGAAGAAACCACCAAAATCACCAGTAATATCCTTAGATTCTGGGTTGGTGTATGTTCGTAGAGTCCAGATCACATCATGTCAAATACATTATTGTGGTCCTGAAGTCAATATCTCAAATCGTGTACTACGCAATTACCCAATGGATACTGATAACTTCATTCGTGTTTCATTTGTTGGAGAGGAGCTTGCAAAAATTTATTCGGCAGATTTGTCTCCTCGTAAGTCCACTACAAATCAGGATGGGAAAACCAAATTCTACCTGAGGATTCTTTCTATTCTCAGAAACGGCATAGTAATTGGTGAAAAGAAATATGAATTTCTTGCATTCTCTTCGAGCCAGTTGCGGGAGAATTCTATGTGGATGTTTGCTTCAAGGTGGGGACTTACAGCCGATGATATAAGAGAGTGGATGGGAGATTTTTGTCGTATAAAAAATGTGGCAAAGTACGCTGCTAGATTGGGTCAATCCTTTGGTTCGTCCACTGAAACTTTGACTGTGGCCAGACATGAAATTGAAGTTATTCCCGACATTGTCCATAATAAATATGTCTTCTCTGATGGGATTGGGAAAATATCTGCTGATTTTGCTAAGAAAGTGGCCTTAAAATGTGGCTATAAAGGGTTTACTCCATCTGCCTTTCAGATTCGATATGGTGGTTACAAAGGTGTGGTGGCCATTGATCCAACATCATCAATGAAATTATCATTAAGGAACAGTATGTCCAAGTATGAATCAGAAAACGCAAAGTTGGATGTTTTAGCATGTAGCAAGTATCAGCCTTGTTTTCTGAACCGCCAGTTGATAACTCTTTTATCTACCCTTGAGGTTCCAGATCTTGTTTTCATGAAAAAACAAAGGGCAGCTGTACAACAACTGAATGCTATATTGACTGACCCACTAAAAGCACAGGAGGCTTTGGATTTGATGTCTCCAGGAGAGACTACTAACATCCTGAAGGAATTGCTCACATGTGGTTATAAACCTGATGTTGAACCATTCCTTTCAATGATGCTGCAAACATTTCGGGCATCAAAGTTGCTAGATTTGCGGCTGAAAACAAGAATCTTTATTCCAGATGGACAAGCAATGATGGGATGTCTAGATGAAACCGGAACATTGGAATATGGAGAGGTGTTTGTGCAATTTTCTGGTAACAGACATCCTGGAAGTGCCTCAAACCAGCAATTCATTGAAGGAAAGGTGGTTGTTGCGAAGAACCCGTGTTTACACCCTGGTGATGTACGTGTTTTAAAGGCTGTGAATGTGCCCGAGTTGCACCATATGGTGGATTGTGTAGTTTTTCCACAAAAAGGATCAAGGCCTCATCCAAATGAATGTTCAGGAAGTGATCTCGATGGAGATATCTACTTTGTCTGTTGGGACCAAGAATTGATTCCTCGGAGACCAATCGAACCTATGGATTATACCCCAGCACCAAGTATCGAATTGGATCATGACGTCACAATGGAGGAAGTTGAGGAATATTTTGTGAACTACATGGTCAACGATAGCTTGGGCATAATTGCCAATGCCCACACTGTTTTTGCAGACAAAGATACTTTAAAAGCAATGAGCGAACCATGTATAGAGCTTGCAAAGCTGTTCTCAATTGCCGTTGACTTTCCAAAAACTGGTGTGCCAGCTGTAATACCTGCACATCTATATGTCAAGGAATTTCCAGATTTCATGGAAAAACATGACAAGCCCACCTATCAATCATGCAATGTGATCGGAGCGCTTTTTCAAGAGGTGAAGGACATTGCGCCTCATGACAGCTCTATCAGATCCTTCACTCGTCAAGTGGCAAAGCAGTCATACGACCCTGACATGGAAGTTGATGGCTTTGAGGATTACATTGAAGATGCTATCTATTACAAAGGCAATTACGATTACAAGTTGGGAAATCTGATGGACTATTATGGGATCAAGACAGAGGCTGAAATACTTAGTGGGAGTGTTATGAGAATGTCAAAATCTTTCACTAAGAGGCGGGATGCAGATTCTATAAACATGGCTGTGAGATCGTTGAGAAAGGAAGCTCGGGCATGGTTCAATGAGAAGGGAACGGGTCTAGATTCCGGAGCTGATGATGTATACGCAAAAGCTTCAGCTTGGTACCATGTCACATATCATCCTGATTATTTTGGCACATACAATGAGGGGCTGAATCGTGACCATTTCATCAGCTTTCCGTGGTGCGTTTATGACAAGCTCGTCCTCATCAAAAAAGATAAAGCAAGTATGAGAAGGTCTCTACACATGTCATCTCTAGAACGCCAATTCCGGAGTGGGTTGCATTTGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1117

Amino Acids

127.01

Weight (kDa)

6.29

Isoelectric Point (pI)

42.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	4 - 80	6.8e-15	RdRP1/2, RRM domain
PH_RDR2	PF24823	102 - 252	1e-43	RDR2 PH-like domain
RDR6_2nd	PF24577	142 - 226	3.8e-07	RNA-dependent RNA polymerase 6 second domain
RdRP_helical	PF26252	268 - 344	4e-09	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	371 - 932	2.6e-194	RNA dependent RNA polymerase
RdRP_head	PF26253	953 - 1096	1.9e-48	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2022
AasI	GACNNNNNNGTC	1 cut(s)	3257
AatII	GACGTC	1 cut(s)	2505
Acc36I	ACCTGC	1 cut(s)	2704
Acc65I	GGTACC	1 cut(s)	3159
AccB1I	GGYRCC	1 cut(s)	3159
AccB7I	CCANNNNNTGG	4 cut(s)	713, 896, 2329, 2675
AccI	GTMKAC	1 cut(s)	3138
AccIII	TCCGGA	3 cut(s)	624, 3122, 3327
AciI	CCGC	7 cut(s)	162, 276, 1036, 1387, 1837, 2092, 3034
AcoI	YGGCCR	3 cut(s)	192, 1542, 1716
AcsI	RAATTY	6 cut(s)	124, 1236, 1295, 1358, 1393, 2717
AcuI	CTGAAG	5 cut(s)	251, 543, 1164, 2018, 3135
AcvI	CACGTG	1 cut(s)	485
AcyI	GRCGYC	1 cut(s)	2502
AfaI	GTAC	8 cut(s)	261, 734, 1034, 1167, 1490, 1911, 2286, 3161
AfeI	AGCGCT	1 cut(s)	2781
AfiI	CCNNNNNNNGG	9 cut(s)	244, 713, 760, 896, 1387, 2329, 2354, 2675, 2872
AflIII	ACRYGT	3 cut(s)	2012, 2287, 3303
AjnI	CCWGG	5 cut(s)	861, 883, 1975, 2206, 2275
AjuI	GAANNNNNNNTTGG	4 cut(s)	2365, 2397, 2421, 2453
AleI	CACNNNNGTG	1 cut(s)	2277
AloI	GAACNNNNNNTCC	4 cut(s)	1748, 1780, 3309, 3341
Alw26I	GTCTC	7 cut(s)	701, 930, 1260, 1976, 1977, 2440, 3300
AlwNI	CAGNNNCTG	2 cut(s)	53, 2981
Ama87I	CYCGRG	3 cut(s)	728, 2312, 3084
Aor13HI	TCCGGA	3 cut(s)	624, 3122, 3327
Aor51HI	AGCGCT	1 cut(s)	2781
AoxI	GGCC	6 cut(s)	192, 887, 1542, 1641, 1716, 2363
ApeKI	GCWGC	3 cut(s)	1493, 1904, 2056
ApoI	RAATTY	6 cut(s)	124, 1236, 1295, 1358, 1393, 2717
AseI	ATTAAT	2 cut(s)	183, 398
Asp700I	GAANNNNTTC	3 cut(s)	723, 1563, 2436
Asp718I	GGTACC	1 cut(s)	3159
AspLEI	GCGC	4 cut(s)	529, 653, 2782, 2809
AspS9I	GGNCC	4 cut(s)	751, 887, 1139, 2425
AsuC2I	CCSGG	3 cut(s)	238, 729, 730
AsuHPI	GGTGA	5 cut(s)	911, 1053, 1355, 2291, 2806
AvaI	CYCGRG	3 cut(s)	728, 2312, 3084
AvaII	GGWCC	3 cut(s)	751, 1139, 2425
AxyI	CCTNAGG	1 cut(s)	1304
BaeGI	GKGCMC	1 cut(s)	2313
BaeI	ACNNNNGTAYC	1 cut(s)	38
BalI	TGGCCA	3 cut(s)	194, 1544, 1718
BamHI	GGATCC	1 cut(s)	621
BanI	GGYRCC	1 cut(s)	3159
BbrPI	CACGTG	1 cut(s)	485
BbsI	GAAGAC	4 cut(s)	398, 582, 657, 1585
BbvI	GCAGC	3 cut(s)	1480, 1916, 2043
BceAI	ACGGC	2 cut(s)	1345, 2642
BcgI	CGANNNNNNTGC	8 cut(s)	1356, 1366, 1390, 1400, 2048, 2082, 2755, 2789
BciT130I	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2277
BciVI	GTATCC	1 cut(s)	1180
BcnI	CCSGG	3 cut(s)	238, 729, 730
BcoDI	GTCTC	7 cut(s)	701, 930, 1260, 1976, 1977, 2440, 3300
BfaI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3116, 3314
BfmI	CTRYAG	1 cut(s)	45
BfoI	RGCGCY	2 cut(s)	530, 2783
BfuAI	ACCTGC	1 cut(s)	2704
BfuI	GTATCC	1 cut(s)	1180
BglII	AGATCT	1 cut(s)	1876
BisI	GCNGC	4 cut(s)	1494, 1905, 2057, 2093
BlsI	GCNGC	4 cut(s)	1495, 1906, 2058, 2094
Bme1390I	CCNGG	8 cut(s)	238, 729, 730, 863, 885, 1977, 2208, 2277
Bme18I	GGWCC	3 cut(s)	751, 1139, 2425
BmeT110I	CYCGRG	3 cut(s)	728, 2312, 3084
BmgT120I	GGNCC	4 cut(s)	751, 887, 1139, 2425
BmiI	GGNNCC	6 cut(s)	623, 752, 799, 1872, 2426, 3161
BmrFI	CCNGG	8 cut(s)	238, 729, 730, 863, 885, 1977, 2208, 2277
BmsI	GCATC	7 cut(s)	50, 160, 913, 2043, 2081, 2896, 3028
BpiI	GAAGAC	4 cut(s)	398, 582, 657, 1585
BplI	GAGNNNNNCTC	2 cut(s)	3280, 3312
BpmI	CTGGAG	2 cut(s)	89, 1959
BpuEI	CTTGAG	2 cut(s)	1034, 1886
BpuMI	CCSGG	3 cut(s)	238, 729, 730
BsaAI	YACGTR	2 cut(s)	485, 2288
BsaHI	GRCGYC	1 cut(s)	2502
BsaI	GGTCTC	4 cut(s)	701, 930, 2440, 3300
BsaJI	CCNNGG	9 cut(s)	189, 530, 728, 801, 861, 884, 2275, 2441, 3236
BsaWI	WCCGGW	5 cut(s)	323, 624, 2153, 3122, 3327
BsaXI	ACNNNNNCTCC	2 cut(s)	789, 819
Bsc4I	CCNNNNNNNGG	9 cut(s)	244, 713, 760, 896, 1387, 2329, 2354, 2675, 2872
Bse1I	ACTGG	6 cut(s)	85, 205, 1064, 1381, 1840, 2680
Bse21I	CCTNAGG	1 cut(s)	1304
Bse3DI	GCAATG	4 cut(s)	2136, 2619, 2773, 2802
BseAI	TCCGGA	3 cut(s)	624, 3122, 3327
BseBI	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2277
BseDI	CCNNGG	9 cut(s)	189, 530, 728, 801, 861, 884, 2275, 2441, 3236
BseLI	CCNNNNNNNGG	9 cut(s)	244, 713, 760, 896, 1387, 2329, 2354, 2675, 2872
BseMI	GCAATG	4 cut(s)	2136, 2619, 2773, 2802
BseMII	CTCAG	4 cut(s)	165, 459, 1295, 1336
BseNI	ACTGG	6 cut(s)	85, 205, 1064, 1381, 1840, 2680
BseRI	GAGGAG	3 cut(s)	1031, 1238, 1248
BseSI	GKGCMC	1 cut(s)	2313
BseXI	GCAGC	3 cut(s)	1480, 1916, 2043
BseYI	CCCAGC	1 cut(s)	2473
BsgI	GTGCAG	1 cut(s)	2682
BshFI	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2365
BshNI	GGYRCC	1 cut(s)	3159
BsiHKCI	CYCGRG	3 cut(s)	728, 2312, 3084
BsiSI	CCGG	7 cut(s)	238, 324, 625, 729, 2154, 3123, 3328
BslFI	GGGAC	5 cut(s)	220, 756, 764, 1437, 2438
BslI	CCNNNNNNNGG	9 cut(s)	244, 713, 760, 896, 1387, 2329, 2354, 2675, 2872
BsmAI	GTCTC	7 cut(s)	701, 930, 1260, 1976, 1977, 2440, 3300
BsmFI	GGGAC	5 cut(s)	220, 756, 764, 1437, 2438
BsmI	GAATGC	2 cut(s)	1367, 1927
BsnI	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2365
Bso31I	GGTCTC	4 cut(s)	701, 930, 2440, 3300
BsoBI	CYCGRG	3 cut(s)	728, 2312, 3084
Bsp1286I	GDGCHC	1 cut(s)	2313
Bsp13I	TCCGGA	3 cut(s)	624, 3122, 3327
Bsp1407I	TGTACA	1 cut(s)	1909
Bsp19I	CCATGG	1 cut(s)	801
BspACI	CCGC	7 cut(s)	162, 276, 1036, 1387, 1837, 2092, 3034
BspANI	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2365
BspCNI	CTCAG	4 cut(s)	166, 460, 1296, 1335
BspEI	TCCGGA	3 cut(s)	624, 3122, 3327
BspHI	TCATGA	4 cut(s)	250, 1887, 2497, 2812
BspLI	GGNNCC	6 cut(s)	623, 752, 799, 1872, 2426, 3161
BspMAI	CTGCAG	1 cut(s)	49
BspMI	ACCTGC	1 cut(s)	2704
BspQI	GCTCTTC	1 cut(s)	327
BspT107I	GGYRCC	1 cut(s)	3159
BspTNI	GGTCTC	4 cut(s)	701, 930, 2440, 3300
BsrDI	GCAATG	4 cut(s)	2136, 2619, 2773, 2802
BsrGI	TGTACA	1 cut(s)	1909
BsrI	ACTGG	6 cut(s)	85, 205, 1064, 1381, 1840, 2680
BssECI	CCNNGG	9 cut(s)	189, 530, 728, 801, 861, 884, 2275, 2441, 3236
BssNAI	GTATAC	1 cut(s)	3139
BssNI	GRCGYC	1 cut(s)	2502
BssT1I	CCWWGG	2 cut(s)	189, 801
Bst1107I	GTATAC	1 cut(s)	3139
Bst2UI	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2277
Bst4CI	ACNGT	5 cut(s)	18, 287, 1540, 1760, 2584
Bst6I	CTCTTC	4 cut(s)	327, 562, 928, 1378
BstACI	GRCGYC	1 cut(s)	2502
BstAPI	GCANNNNNTGC	1 cut(s)	289
BstAUI	TGTACA	1 cut(s)	1909
BstBAI	YACGTR	2 cut(s)	485, 2288
BstC8I	GCNNGC	5 cut(s)	928, 946, 1230, 2637, 2685
BstDEI	CTNAG	9 cut(s)	174, 468, 498, 1075, 1304, 1322, 1632, 2990, 3027
BstDSI	CCRYGG	2 cut(s)	801, 3236
BstH2I	RGCGCY	2 cut(s)	530, 2783
BstHHI	GCGC	4 cut(s)	529, 653, 2782, 2809
BstMAI	GTCTC	7 cut(s)	701, 930, 1260, 1976, 1977, 2440, 3300
BstNI	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2277
BstNSI	RCATGY	4 cut(s)	948, 1809, 2016, 3307
BstSCI	CCNGG	8 cut(s)	236, 727, 728, 861, 883, 1975, 2206, 2275
BstSFI	CTRYAG	1 cut(s)	45
BstSLI	GKGCMC	1 cut(s)	2313
BstV1I	GCAGC	3 cut(s)	1480, 1916, 2043
BstV2I	GAAGAC	4 cut(s)	398, 582, 657, 1585
BstX2I	RGATCY	5 cut(s)	89, 621, 790, 1876, 2828
BstYI	RGATCY	5 cut(s)	89, 621, 790, 1876, 2828
BstZ17I	GTATAC	1 cut(s)	3139
Bsu36I	CCTNAGG	1 cut(s)	1304
BsuI	GTATCC	1 cut(s)	1180
BsuRI	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2365
BtgI	CCRYGG	2 cut(s)	801, 3236
BtsI	GCAGTG	1 cut(s)	492
BtsIMutI	CAGTG	5 cut(s)	198, 375, 492, 1524, 2580
BveI	ACCTGC	1 cut(s)	2704
Cac8I	GCNNGC	5 cut(s)	928, 946, 1230, 2637, 2685
CaiI	CAGNNNCTG	2 cut(s)	53, 2981
CciI	TCATGA	4 cut(s)	250, 1887, 2497, 2812
CfoI	GCGC	4 cut(s)	529, 653, 2782, 2809
Cfr13I	GGNCC	4 cut(s)	751, 887, 1139, 2425
Cfr9I	CCCGGG	1 cut(s)	728
Csp6I	GTAC	8 cut(s)	260, 733, 1033, 1166, 1489, 1910, 2285, 3160
CviQI	GTAC	8 cut(s)	260, 733, 1033, 1166, 1489, 1910, 2285, 3160
DdeI	CTNAG	9 cut(s)	174, 468, 498, 1075, 1304, 1322, 1632, 2990, 3027
DraI	TTTAAA	2 cut(s)	2295, 2607
DrdI	GACNNNNNNGTC	1 cut(s)	3257
DseDI	GACNNNNNNGTC	1 cut(s)	3257
EaeI	YGGCCR	3 cut(s)	192, 1542, 1716
Eam1104I	CTCTTC	4 cut(s)	327, 562, 928, 1378
EarI	CTCTTC	4 cut(s)	327, 562, 928, 1378
Eco130I	CCWWGG	2 cut(s)	189, 801
Eco147I	AGGCCT	1 cut(s)	2365
Eco31I	GGTCTC	4 cut(s)	701, 930, 2440, 3300
Eco32I	GATATC	2 cut(s)	841, 2407
Eco47I	GGWCC	3 cut(s)	751, 1139, 2425
Eco47III	AGCGCT	1 cut(s)	2781
Eco57I	CTGAAG	5 cut(s)	251, 543, 1164, 2018, 3135
Eco72I	CACGTG	1 cut(s)	485
Eco81I	CCTNAGG	1 cut(s)	1304
Eco88I	CYCGRG	3 cut(s)	728, 2312, 3084
EcoO109I	RGGNCCY	1 cut(s)	751
EcoRI	GAATTC	1 cut(s)	1393
EcoRII	CCWGG	5 cut(s)	861, 883, 1975, 2206, 2275
EcoRV	GATATC	2 cut(s)	841, 2407
EcoT14I	CCWWGG	2 cut(s)	189, 801
EcoT22I	ATGCAT	1 cut(s)	950
ErhI	CCWWGG	2 cut(s)	189, 801
FalI	AAGNNNNNCTT	4 cut(s)	262, 294, 864, 896
FaqI	GGGAC	5 cut(s)	220, 756, 764, 1437, 2438
FauI	CCCGC	2 cut(s)	1380, 3027
FauNDI	CATATG	3 cut(s)	571, 835, 2325
FblI	GTMKAC	1 cut(s)	3138
Fnu4HI	GCNGC	4 cut(s)	1494, 1905, 2057, 2093
Fsp4HI	GCNGC	4 cut(s)	1494, 1905, 2057, 2093
FspBI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3116, 3314
GlaI	GCGC	4 cut(s)	528, 652, 2781, 2808
GluI	GCNGC	4 cut(s)	1494, 1905, 2057, 2093
GsaI	CCCAGC	1 cut(s)	2477
GsuI	CTGGAG	2 cut(s)	89, 1959
HaeII	RGCGCY	2 cut(s)	530, 2783
HaeIII	GGCC	6 cut(s)	194, 889, 1544, 1643, 1718, 2365
HapII	CCGG	7 cut(s)	238, 324, 625, 729, 2154, 3123, 3328
HhaI	GCGC	4 cut(s)	529, 653, 2782, 2809
Hin1I	GRCGYC	1 cut(s)	2502
Hin6I	GCGC	4 cut(s)	527, 651, 2780, 2807
HinP1I	GCGC	4 cut(s)	527, 651, 2780, 2807
HincII	GTYRAC	2 cut(s)	2548, 2662
HindII	GTYRAC	2 cut(s)	2548, 2662
HindIII	AAGCTT	2 cut(s)	434, 3147
HpaII	CCGG	7 cut(s)	238, 324, 625, 729, 2154, 3123, 3328
HphI	GGTGA	5 cut(s)	911, 1053, 1355, 2291, 2806
HpyAV	CCTTC	8 cut(s)	42, 1011, 1993, 2231, 2791, 2842, 3097, 3285
HpyCH4III	ACNGT	5 cut(s)	18, 287, 1540, 1760, 2584
HpyCH4IV	ACGT	3 cut(s)	484, 2287, 2502
HpyF3I	CTNAG	9 cut(s)	174, 468, 498, 1075, 1304, 1322, 1632, 2990, 3027
HpySE526I	ACGT	3 cut(s)	484, 2287, 2502
Hsp92I	GRCGYC	1 cut(s)	2502
HspAI	GCGC	4 cut(s)	527, 651, 2780, 2807
Kpn2I	TCCGGA	3 cut(s)	624, 3122, 3327
KpnI	GGTACC	1 cut(s)	3163
LguI	GCTCTTC	1 cut(s)	327
LmnI	GCTCC	7 cut(s)	524, 682, 797, 1018, 1225, 2777, 3125
Lsp1109I	GCAGC	3 cut(s)	1480, 1916, 2043
LweI	GCATC	7 cut(s)	50, 160, 913, 2043, 2081, 2896, 3028
MaeI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3116, 3314
MaeII	ACGT	3 cut(s)	484, 2287, 2502
MaeIII	GTNAC	7 cut(s)	488, 757, 1700, 2195, 2503, 3166, 3215
MfeI	CAATTG	1 cut(s)	2652
MflI	RGATCY	5 cut(s)	89, 621, 790, 1876, 2828
MhlI	GDGCHC	1 cut(s)	2313
MlsI	TGGCCA	3 cut(s)	194, 1544, 1718
MluNI	TGGCCA	3 cut(s)	194, 1544, 1718
MlyI	GAGTC	2 cut(s)	548, 1113
MmeI	TCCRAC	4 cut(s)	724, 1198, 1751, 1773
Mox20I	TGGCCA	3 cut(s)	194, 1544, 1718
Mph1103I	ATGCAT	1 cut(s)	950
MroI	TCCGGA	3 cut(s)	624, 3122, 3327
MroXI	GAANNNNTTC	3 cut(s)	723, 1563, 2436
MscI	TGGCCA	3 cut(s)	194, 1544, 1718
MseI	TTAA	9 cut(s)	183, 398, 746, 849, 980, 1646, 1751, 2294, 2606
MslI	CAYNNNNRTG	5 cut(s)	1134, 2277, 2373, 2706, 3059
Msp20I	TGGCCA	3 cut(s)	194, 1544, 1718
MspA1I	CMGCKG	2 cut(s)	1907, 2687
MspI	CCGG	7 cut(s)	238, 324, 625, 729, 2154, 3123, 3328
MspR9I	CCNGG	8 cut(s)	238, 729, 730, 863, 885, 1977, 2208, 2277
MunI	CAATTG	1 cut(s)	2652
Mva1269I	GAATGC	2 cut(s)	1367, 1927
MvaI	CCWGG	5 cut(s)	863, 885, 1977, 2208, 2277
NciI	CCSGG	3 cut(s)	238, 729, 730
NcoI	CCATGG	1 cut(s)	801
NdeI	CATATG	3 cut(s)	571, 835, 2325
NlaIV	GGNNCC	6 cut(s)	623, 752, 799, 1872, 2426, 3161
NmuCI	GTSAC	5 cut(s)	488, 757, 2503, 3166, 3215
NsiI	ATGCAT	1 cut(s)	950
NspI	RCATGY	4 cut(s)	948, 1809, 2016, 3307
OliI	CACNNNNGTG	1 cut(s)	2277
PaeI	GCATGC	1 cut(s)	948
PagI	TCATGA	4 cut(s)	250, 1887, 2497, 2812
PceI	AGGCCT	1 cut(s)	2365
PciI	ACATGT	2 cut(s)	2012, 3303
PciSI	GCTCTTC	1 cut(s)	327
PctI	GAATGC	2 cut(s)	1367, 1927
PdmI	GAANNNNTTC	3 cut(s)	723, 1563, 2436
PflFI	GACNNNGTC	2 cut(s)	755, 1574
PflMI	CCANNNNNTGG	4 cut(s)	713, 896, 2329, 2675
PfoI	TCCNGGA	3 cut(s)	236, 1975, 2206
PkrI	GCNGC	4 cut(s)	1495, 1906, 2058, 2094
PleI	GAGTC	2 cut(s)	548, 1112
PmaCI	CACGTG	1 cut(s)	485
PmlI	CACGTG	1 cut(s)	485
PpsI	GAGTC	2 cut(s)	548, 1112
Ppu21I	YACGTR	2 cut(s)	485, 2288
PpuMI	RGGWCCY	1 cut(s)	751
PscI	ACATGT	2 cut(s)	2012, 3303
PshBI	ATTAAT	2 cut(s)	183, 398
PsiI	TTATAA	1 cut(s)	2022
Psp5II	RGGWCCY	1 cut(s)	751
Psp6I	CCWGG	5 cut(s)	861, 883, 1975, 2206, 2275
PspCI	CACGTG	1 cut(s)	485
PspFI	CCCAGC	1 cut(s)	2473
PspGI	CCWGG	5 cut(s)	861, 883, 1975, 2206, 2275
PspN4I	GGNNCC	6 cut(s)	623, 752, 799, 1872, 2426, 3161
PspPI	GGNCC	4 cut(s)	751, 887, 1139, 2425
PspPPI	RGGWCCY	1 cut(s)	751
PstI	CTGCAG	1 cut(s)	49
PstNI	CAGNNNCTG	2 cut(s)	53, 2981
PsuI	RGATCY	5 cut(s)	89, 621, 790, 1876, 2828
PsyI	GACNNNGTC	2 cut(s)	755, 1574
PvuII	CAGCTG	2 cut(s)	1907, 2687
RsaI	GTAC	8 cut(s)	261, 734, 1034, 1167, 1490, 1911, 2286, 3161
RsaNI	GTAC	8 cut(s)	260, 733, 1033, 1166, 1489, 1910, 2285, 3160
RseI	CAYNNNNRTG	5 cut(s)	1134, 2277, 2373, 2706, 3059
SapI	GCTCTTC	1 cut(s)	327
SaqAI	TTAA	9 cut(s)	183, 398, 746, 849, 980, 1646, 1751, 2294, 2606
SatI	GCNGC	4 cut(s)	1494, 1905, 2057, 2093
Sau96I	GGNCC	4 cut(s)	751, 887, 1139, 2425
SchI	GAGTC	2 cut(s)	548, 1113
ScrFI	CCNGG	8 cut(s)	238, 729, 730, 863, 885, 1977, 2208, 2277
SduI	GDGCHC	1 cut(s)	2313
SfaNI	GCATC	7 cut(s)	50, 160, 913, 2043, 2081, 2896, 3028
SfcI	CTRYAG	1 cut(s)	45
SinI	GGWCC	3 cut(s)	751, 1139, 2425
SmaI	CCCGGG	1 cut(s)	730
SmiMI	CAYNNNNRTG	5 cut(s)	1134, 2277, 2373, 2706, 3059
SmlI	CTYRAG	2 cut(s)	1013, 1865
SmoI	CTYRAG	2 cut(s)	1013, 1865
SphI	GCATGC	1 cut(s)	948
SseBI	AGGCCT	1 cut(s)	2365
SsiI	CCGC	7 cut(s)	162, 276, 1036, 1387, 1837, 2092, 3034
SspI	AATATT	1 cut(s)	2528
SspMI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3116, 3314
StuI	AGGCCT	1 cut(s)	2365
StyD4I	CCNGG	8 cut(s)	236, 727, 728, 861, 883, 1975, 2206, 2275
StyI	CCWWGG	2 cut(s)	189, 801
TaaI	ACNGT	5 cut(s)	18, 287, 1540, 1760, 2584
TaiI	ACGT	3 cut(s)	487, 2290, 2505
TaqI	TCGA	6 cut(s)	540, 1376, 1690, 2397, 2454, 2487
TatI	WGTACW	2 cut(s)	1165, 1909
TauI	GCSGC	1 cut(s)	2095
Tru1I	TTAA	9 cut(s)	183, 398, 746, 849, 980, 1646, 1751, 2294, 2606
Tru9I	TTAA	9 cut(s)	183, 398, 746, 849, 980, 1646, 1751, 2294, 2606
TscAI	CASTG	5 cut(s)	205, 375, 499, 1531, 2587
TseFI	GTSAC	5 cut(s)	488, 757, 2503, 3166, 3215
TseI	GCWGC	3 cut(s)	1493, 1904, 2056
Tsp45I	GTSAC	5 cut(s)	488, 757, 2503, 3166, 3215
TspGWI	ACGGA	3 cut(s)	406, 776, 3225
TspMI	CCCGGG	1 cut(s)	728
TspRI	CASTG	5 cut(s)	205, 375, 499, 1531, 2587
Tth111I	GACNNNGTC	2 cut(s)	755, 1574
Van91I	CCANNNNNTGG	4 cut(s)	713, 896, 2329, 2675
VpaK11BI	GGWCC	3 cut(s)	751, 1139, 2425
VspI	ATTAAT	2 cut(s)	183, 398
XapI	RAATTY	6 cut(s)	124, 1236, 1295, 1358, 1393, 2717
XbaI	TCTAGA	4 cut(s)	961, 2143, 3115, 3313
XceI	RCATGY	4 cut(s)	948, 1809, 2016, 3307
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3329
XmaI	CCCGGG	1 cut(s)	728
XmiI	GTMKAC	1 cut(s)	3138
XmnI	GAANNNNTTC	3 cut(s)	723, 1563, 2436
XspI	CTAG	7 cut(s)	299, 962, 1497, 2084, 2144, 3116, 3314
ZraI	GACGTC	1 cut(s)	2503
Zsp2I	ATGCAT	1 cut(s)	950

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.