pycom16g16570
ERF Family

Bifunctional inhibitor lipid-transfer protein seed storage 2S albumin superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005475
Physical Location & Seq
Forward (+)
12453588 .. 12455167
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGCAATTGATTCCAACGTTCTTGAAGTTCTGTGCGGAGAGAAGAGTGAAGTTAGACGGATTCGAAGTTCGAATCTGTAACCATGTGGTTATCTGCAAATTTGAGAATTTCATGATGACCACCACTACTGCTCCTCCTCCTCTCATGGCCATCCTCCTAGCCTTGACGCTCGTCATTCTCGGCTTGGAATCAGCAGAACCGCCGCCGTCTCCGCCACCTAGTTGCGCCGACGAGCTCGTGAGATTCTCGCCGTGCCTGCCTTACGTGTCGTCTCCGCCAAACAACCTCTCCGACTCACCCCCACCCAAGTGCTGCGACGCGTTCTCGCTGTCGATGGAGTCCGGCGGCGCGCTCTGCCTTTGTTACCTGGTCCAGGATCCTCCGATGCTGGGGTTTCCAGTAAACGGGAGTCGCGTTCTGTCTCTGTCTTCCACTTGCCCTCTCAGAGACATTTCCACAAACGCAAGTGCACAATATTTGGAGTCTCTCTGCTCAGGATCACCAGAACTCCCTCCTCTCCGCAGCTCAACAATTTCAGTGATTTCAAGTCCTCCTCCTTCAGGTTTTGATTCTGTGGACAATGCTTCATCTCCTCGCATGAACTTAGCACCAGAACCAGCATACAATTCAAGCATTCCGCGGGGAAACAGATCGCCAACTCCGCCAAGCTCAGCGGTAGAACCAGCAAGGATTTCAGCTGCAATGAAGCAAGTGTACTTGAGCAACTCTTGGATTCTACCTGCCATTGTGAGCTTTCTTGTTCAGACATTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

259

Amino Acids

27.6

Weight (kDa)

5.21

Isoelectric Point (pI)

67.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 65 - 149 2e-12 Probable lipid transfer
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 748
AccII CGCG 4 cut(s) 320, 350, 414, 640
AclI AACGTT 1 cut(s) 17
AclWI GGATC 3 cut(s) 371, 384, 505
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 2 cut(s) 98, 106
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 1 cut(s) 716
AfiI CCNNNNNNNGG 4 cut(s) 373, 389, 404, 560
AflIII ACRYGT 2 cut(s) 264, 318
AgsI TTSAA 3 cut(s) 25, 546, 630
AjnI CCWGG 2 cut(s) 366, 372
AleI CACNNNNGTG 1 cut(s) 307
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
AluBI AGCT 5 cut(s) 235, 525, 669, 698, 753
AluI AGCT 5 cut(s) 235, 525, 669, 698, 753
Alw21I GWGCWC 2 cut(s) 237, 472
Alw26I GTCTC 5 cut(s) 213, 276, 426, 441, 489
Alw44I GTGCAC 1 cut(s) 468
AlwI GGATC 3 cut(s) 371, 384, 505
AoxI GGCC 1 cut(s) 147
ApaLI GTGCAC 1 cut(s) 468
ApeKI GCWGC 3 cut(s) 312, 522, 698
ApoI RAATTY 2 cut(s) 98, 106
AspLEI GCGC 3 cut(s) 227, 350, 352
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 2 cut(s) 288, 492
AsuII TTCGAA 2 cut(s) 63, 70
AvaII GGWCC 1 cut(s) 370
BaeGI GKGCMC 1 cut(s) 472
BalI TGGCCA 1 cut(s) 149
BamHI GGATCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 698, 730
BauI CACGAG 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 237, 472
BbvI GCAGC 3 cut(s) 299, 534, 685
BccI CCATC 2 cut(s) 158, 328
BceAI ACGGC 2 cut(s) 190, 235
BciT130I CCWGG 2 cut(s) 368, 374
BcoDI GTCTC 5 cut(s) 213, 276, 426, 441, 489
BfaI CTAG 2 cut(s) 158, 219
BfuAI ACCTGC 1 cut(s) 748
BisI GCNGC 5 cut(s) 203, 313, 346, 523, 699
BlpI GCTNAGC 1 cut(s) 670
BlsI GCNGC 5 cut(s) 204, 314, 347, 524, 700
Bme1390I CCNGG 2 cut(s) 368, 374
Bme18I GGWCC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 2 cut(s) 368, 374
BmsI GCATC 1 cut(s) 375
BoxI GACNNNNGTC 1 cut(s) 170
BpiI GAAGAC 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 493
Bpu1102I GCTNAGC 1 cut(s) 670
Bpu14I TTCGAA 2 cut(s) 63, 70
BpuEI CTTGAG 1 cut(s) 739
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 638
BsaXI ACNNNNNCTCC 8 cut(s) 115, 118, 145, 148, 272, 302, 492, 522
Bsc4I CCNNNNNNNGG 4 cut(s) 373, 389, 404, 560
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 708
BseBI CCWGG 2 cut(s) 368, 374
BseDI CCNNGG 1 cut(s) 638
BseGI GGATG 1 cut(s) 150
BseLI CCNNNNNNNGG 4 cut(s) 373, 389, 404, 560
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 3 cut(s) 457, 507, 684
BseNI ACTGG 1 cut(s) 398
BsePI GCGCGC 1 cut(s) 348
BseRI GAGGAG 6 cut(s) 123, 126, 129, 504, 543, 582
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 3 cut(s) 299, 534, 685
BseYI CCCAGC 1 cut(s) 388
Bsh1236I CGCG 4 cut(s) 320, 350, 414, 640
BshFI GGCC 1 cut(s) 149
BsiHKAI GWGCWC 2 cut(s) 237, 472
BsiSI CCGG 1 cut(s) 342
BslI CCNNNNNNNGG 4 cut(s) 373, 389, 404, 560
BsmAI GTCTC 5 cut(s) 213, 276, 426, 441, 489
BsmBI CGTCTC 2 cut(s) 213, 276
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 1 cut(s) 149
Bsp119I TTCGAA 2 cut(s) 63, 70
Bsp1286I GDGCHC 2 cut(s) 237, 472
Bsp143I GATC 3 cut(s) 376, 497, 650
Bsp1720I GCTNAGC 1 cut(s) 670
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 3 cut(s) 456, 506, 683
BspFNI CGCG 4 cut(s) 320, 350, 414, 640
BspHI TCATGA 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 378
BspMI ACCTGC 1 cut(s) 748
BspPI GGATC 3 cut(s) 371, 384, 505
BspT104I TTCGAA 2 cut(s) 63, 70
BsrDI GCAATG 1 cut(s) 708
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 638
BssHII GCGCGC 1 cut(s) 348
BssMI GATC 3 cut(s) 376, 497, 650
BssSI CACGAG 1 cut(s) 236
Bst2BI CACGAG 1 cut(s) 236
Bst2UI CCWGG 2 cut(s) 368, 374
Bst6I CTCTTC 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 265
BstBI TTCGAA 2 cut(s) 63, 70
BstC8I GCNNGC 2 cut(s) 257, 350
BstDEI CTNAG 4 cut(s) 443, 493, 604, 670
BstDSI CCRYGG 1 cut(s) 638
BstENI CCTNNNNNAGG 2 cut(s) 371, 558
BstF5I GGATG 1 cut(s) 150
BstFNI CGCG 4 cut(s) 320, 350, 414, 640
BstHHI GCGC 3 cut(s) 227, 350, 352
BstKTI GATC 3 cut(s) 379, 500, 653
BstMAI GTCTC 5 cut(s) 213, 276, 426, 441, 489
BstMBI GATC 3 cut(s) 376, 497, 650
BstMWI GCNNNNNNNGC 4 cut(s) 211, 256, 354, 661
BstNI CCWGG 2 cut(s) 368, 374
BstPAI GACNNNNGTC 1 cut(s) 170
BstSCI CCNGG 2 cut(s) 366, 372
BstSLI GKGCMC 1 cut(s) 472
BstUI CGCG 4 cut(s) 320, 350, 414, 640
BstV1I GCAGC 3 cut(s) 299, 534, 685
BstV2I GAAGAC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 638
BtsCI GGATG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 543
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 2 cut(s) 257, 350
CciI TCATGA 1 cut(s) 111
CfoI GCGC 3 cut(s) 227, 350, 352
Cfr13I GGNCC 1 cut(s) 370
Cfr42I CCGCGG 1 cut(s) 641
CseI GACGC 2 cut(s) 175, 326
CsiI ACCWGGT 1 cut(s) 366
Csp6I GTAC 1 cut(s) 715
CviAII CATG 4 cut(s) 83, 112, 145, 598
CviJI RGCY 8 cut(s) 149, 161, 183, 235, 525, 669, 698, 753
CviKI_1 RGCY 8 cut(s) 149, 161, 183, 235, 525, 669, 698, 753
CviQI GTAC 1 cut(s) 715
DdeI CTNAG 4 cut(s) 443, 493, 604, 670
DpnI GATC 3 cut(s) 378, 499, 652
DpnII GATC 3 cut(s) 376, 497, 650
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
EciI GGCGGA 3 cut(s) 201, 264, 651
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 543
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 2 cut(s) 371, 558
EcoRII CCWGG 2 cut(s) 366, 372
EcoT38I GRGCYC 1 cut(s) 237
Esp3I CGTCTC 2 cut(s) 213, 276
FaeI CATG 4 cut(s) 86, 115, 148, 601
FaiI YATR 5 cut(s) 84, 113, 146, 599, 622
FatI CATG 4 cut(s) 82, 111, 144, 597
FauI CCCGC 1 cut(s) 633
Fnu4HI GCNGC 5 cut(s) 203, 313, 346, 523, 699
FokI GGATG 1 cut(s) 137
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 5 cut(s) 203, 313, 346, 523, 699
FspBI CTAG 2 cut(s) 158, 219
GlaI GCGC 3 cut(s) 226, 349, 351
GluI GCNGC 5 cut(s) 203, 313, 346, 523, 699
GsaI CCCAGC 1 cut(s) 392
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 342
HgaI GACGC 2 cut(s) 175, 326
HhaI GCGC 3 cut(s) 227, 350, 352
Hin1II CATG 4 cut(s) 86, 115, 148, 601
Hin6I GCGC 3 cut(s) 225, 348, 350
HinP1I GCGC 3 cut(s) 225, 348, 350
HpaII CCGG 1 cut(s) 342
HphI GGTGA 2 cut(s) 288, 492
Hpy166II GTNNAC 4 cut(s) 403, 470, 577, 715
Hpy188I TCNGA 5 cut(s) 292, 384, 446, 765, 776
Hpy188III TCNNGA 4 cut(s) 22, 112, 238, 495
Hpy8I GTNNAC 4 cut(s) 403, 470, 577, 715
Hpy99I CGWCG 2 cut(s) 233, 320
HpyAV CCTTC 1 cut(s) 567
HpyCH4IV ACGT 2 cut(s) 17, 264
HpyCH4V TGCA 4 cut(s) 4, 96, 470, 701
HpyF10VI GCNNNNNNNGC 4 cut(s) 211, 256, 354, 661
HpyF3I CTNAG 4 cut(s) 443, 493, 604, 670
HpySE526I ACGT 2 cut(s) 17, 264
Hsp92II CATG 4 cut(s) 86, 115, 148, 601
HspAI GCGC 3 cut(s) 225, 348, 350
KspI CCGCGG 1 cut(s) 641
Kzo9I GATC 3 cut(s) 376, 497, 650
LmnI GCTCC 1 cut(s) 136
Lsp1109I GCAGC 3 cut(s) 299, 534, 685
LweI GCATC 1 cut(s) 375
MabI ACCWGGT 1 cut(s) 366
MaeI CTAG 2 cut(s) 158, 219
MaeII ACGT 2 cut(s) 17, 264
MaeIII GTNAC 2 cut(s) 77, 362
MalI GATC 3 cut(s) 378, 499, 652
MboI GATC 3 cut(s) 376, 497, 650
MboII GAAGA 2 cut(s) 54, 420
MfeI CAATTG 1 cut(s) 5
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 2 cut(s) 237, 472
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 5 cut(s) 5, 98, 106, 531, 625
MluI ACGCGT 1 cut(s) 318
MluNI TGGCCA 1 cut(s) 149
MlyI GAGTC 4 cut(s) 287, 347, 418, 491
MmeI TCCRAC 2 cut(s) 38, 315
Mox20I TGGCCA 1 cut(s) 149
MscI TGGCCA 1 cut(s) 149
MslI CAYNNNNRTG 1 cut(s) 307
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 3 cut(s) 640, 674, 698
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 2 cut(s) 368, 374
MunI CAATTG 1 cut(s) 5
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 2 cut(s) 368, 374
MvnI CGCG 4 cut(s) 320, 350, 414, 640
MwoI GCNNNNNNNGC 4 cut(s) 211, 256, 354, 661
NdeII GATC 3 cut(s) 376, 497, 650
NlaIII CATG 4 cut(s) 86, 115, 148, 601
NlaIV GGNNCC 1 cut(s) 378
NmeAIII GCCGAG 1 cut(s) 159
NspV TTCGAA 2 cut(s) 63, 70
OliI CACNNNNGTG 1 cut(s) 307
PagI TCATGA 1 cut(s) 111
PauI GCGCGC 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PctI GAATGC 1 cut(s) 633
PfeI GAWTC 7 cut(s) 10, 60, 72, 188, 243, 569, 733
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 5 cut(s) 204, 314, 347, 524, 700
PleI GAGTC 4 cut(s) 287, 346, 417, 490
PpsI GAGTC 4 cut(s) 287, 346, 417, 490
Ppu21I YACGTR 1 cut(s) 265
PshAI GACNNNNGTC 1 cut(s) 170
Psp124BI GAGCTC 1 cut(s) 237
Psp1406I AACGTT 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 366, 372
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 2 cut(s) 366, 372
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 370
PsuI RGATCY 1 cut(s) 376
PteI GCGCGC 1 cut(s) 348
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 1 cut(s) 716
RsaNI GTAC 1 cut(s) 715
RseI CAYNNNNRTG 1 cut(s) 307
SacI GAGCTC 1 cut(s) 237
SacII CCGCGG 1 cut(s) 641
SatI GCNGC 5 cut(s) 203, 313, 346, 523, 699
Sau3AI GATC 3 cut(s) 376, 497, 650
Sau96I GGNCC 1 cut(s) 370
SchI GAGTC 4 cut(s) 287, 347, 418, 491
ScrFI CCNGG 2 cut(s) 368, 374
SduI GDGCHC 2 cut(s) 237, 472
SexAI ACCWGGT 1 cut(s) 366
SfaNI GCATC 1 cut(s) 375
Sfr303I CCGCGG 1 cut(s) 641
SfuI TTCGAA 2 cut(s) 63, 70
SgrBI CCGCGG 1 cut(s) 641
SinI GGWCC 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 307
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
Sse9I AATT 5 cut(s) 5, 98, 106, 531, 625
SspI AATATT 1 cut(s) 476
SspMI CTAG 2 cut(s) 158, 219
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 2 cut(s) 366, 372
TaiI ACGT 2 cut(s) 20, 267
TaqI TCGA 3 cut(s) 63, 70, 332
TasI AATT 5 cut(s) 5, 98, 106, 531, 625
TatI WGTACW 1 cut(s) 714
TauI GCSGC 2 cut(s) 205, 348
TfiI GAWTC 7 cut(s) 10, 60, 72, 188, 243, 569, 733
TscAI CASTG 1 cut(s) 543
TseI GCWGC 3 cut(s) 312, 522, 698
TspDTI ATGAA 5 cut(s) 100, 576, 614, 719, 760
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 1 cut(s) 543
VneI GTGCAC 1 cut(s) 468
VpaK11BI GGWCC 1 cut(s) 370
XagI CCTNNNNNAGG 2 cut(s) 371, 558
XapI RAATTY 2 cut(s) 98, 106
XspI CTAG 2 cut(s) 158, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.