pycom16g20480

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003166
Physical Location & Seq
Forward (+)
18244176 .. 18248506
4331 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 948 bp
ATGAAATCAAACTCTCTAGTCGCTTATAGCGATAATCAAAACTTAAAACCTGTCACTACTCGAATATCCTTAATAACAGGAAGAAACTCGATCCCCTTCATCTCTCGCGCTGATTCGAAATCAGTAAACAAAGAGAACCCATTTCCAGATTTCAGCAGTGAGAGACTGAAGAACGATTCAGTTGGAATTTTGTTTGGGAGAATTAAAAGTTGGGATTTTGGGATGAGGATCAGTAAAACAGAGGTGAATTTGAGGAGGCTGCTTGCATCTGCGCCCCAGCAACATAACCAGGCAAAACTTGTACATTATGTTGCTACATTACGGGAACTGGTAGAACAACTAGCTGAAGAGAGAAATCCAGAAGGCTTACCAAGAGTTTCAAAGGCTGTTATGAGTGACTATTCAGAGAAGATCGAAGCCATTGCTTCCAATTTAGCTGCTCCGCTGACAGTTTATAGTTCAATGGTCCAGATTAACAGCTTGGCAAGAGAATATTTCTTTAGAATCTCTTCTGATACAGGAGACAATCAGATTCCCGCTTCTCCAGGTCTGAGAAGGAGATTTGTGCCTCCCTCAAATATCAAAGATGGAGCTCGTGAGACTGTTAATGTTGATTCTTCGGCACCTGTTAAACTGGATGCTGCAGCCCAAGCACACATTGAAAAGCATAGAAAGCTTCAAGAGGATTTGACAGACGAAATGGTTGGATTGGCTAGGCAACTCAAAGAGAGTAGTCTCATGATGAGTCACTCCCTGGAGAACACTGAAAAAGTACTTGATTCCACAGAGAAGGCTGTTGAAAGTAGCTTGGCAAGCACTGGCCATGCCACCACACGGGCGACAGATATGTACTCAAAGACGTCCAAAACTACTTGTTTCACATGGCTTCTGATGTTTGTAATGACATGCGTATTCATCATGGTGGTTCTTTTAATTCGCGTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Use1

Protein Analysis

316

Amino Acids

35.13

Weight (kDa)

9.11

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Use1 PF09753 79 - 315 2.6e-66 Membrane fusion protein Use1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 622
AccII CGCG 2 cut(s) 108, 939
AciI CCGC 2 cut(s) 443, 537
AclWI GGATC 2 cut(s) 85, 236
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 2 cut(s) 186, 247
AcuI CTGAAG 2 cut(s) 188, 366
AcyI GRCGYC 1 cut(s) 860
AfaI GTAC 3 cut(s) 303, 774, 851
AfiI CCNNNNNNNGG 1 cut(s) 834
AgsI TTSAA 5 cut(s) 381, 462, 662, 680, 800
AjnI CCWGG 3 cut(s) 288, 544, 753
AjuI GAANNNNNNNTTGG 2 cut(s) 193, 225
AluBI AGCT 6 cut(s) 344, 437, 480, 593, 676, 807
AluI AGCT 6 cut(s) 344, 437, 480, 593, 676, 807
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 4 cut(s) 157, 516, 593, 740
AlwI GGATC 2 cut(s) 85, 236
AoxI GGCC 1 cut(s) 820
ApeKI GCWGC 4 cut(s) 259, 437, 641, 644
ApoI RAATTY 2 cut(s) 186, 247
Asp700I GAANNNNTTC 1 cut(s) 508
AspLEI GCGC 2 cut(s) 110, 274
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 256
AsuII TTCGAA 1 cut(s) 116
AvaII GGWCC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 822
BanI GGYRCC 1 cut(s) 622
BanII GRGCYC 1 cut(s) 595
BauI CACGAG 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 4 cut(s) 246, 424, 628, 656
BccI CCATC 1 cut(s) 581
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 3 cut(s) 290, 546, 755
BcoDI GTCTC 4 cut(s) 157, 516, 593, 740
BfaI CTAG 3 cut(s) 17, 341, 714
BfmI CTRYAG 1 cut(s) 642
BisI GCNGC 4 cut(s) 260, 438, 642, 645
BlsI GCNGC 4 cut(s) 261, 439, 643, 646
BmcAI AGTACT 1 cut(s) 774
Bme1390I CCNGG 3 cut(s) 290, 546, 755
Bme18I GGWCC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 1 cut(s) 624
BmrFI CCNGG 3 cut(s) 290, 546, 755
BmsI GCATC 2 cut(s) 275, 628
BpmI CTGGAG 2 cut(s) 528, 776
Bpu14I TTCGAA 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 860
BsaJI CCNNGG 1 cut(s) 753
Bsc4I CCNNNNNNNGG 1 cut(s) 834
Bse1I ACTGG 3 cut(s) 333, 639, 823
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 3 cut(s) 290, 546, 755
BseDI CCNNGG 1 cut(s) 753
BseGI GGATG 2 cut(s) 228, 643
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 834
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 3 cut(s) 333, 639, 823
BseRI GAGGAG 1 cut(s) 268
BseXI GCAGC 4 cut(s) 246, 424, 628, 656
BseYI CCCAGC 1 cut(s) 276
Bsh1236I CGCG 2 cut(s) 108, 939
BshFI GGCC 1 cut(s) 822
BshNI GGYRCC 1 cut(s) 622
BsiHKAI GWGCWC 1 cut(s) 595
BslI CCNNNNNNNGG 1 cut(s) 834
BsmAI GTCTC 4 cut(s) 157, 516, 593, 740
BsnI GGCC 1 cut(s) 822
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 595
Bsp1407I TGTACA 1 cut(s) 301
Bsp143I GATC 3 cut(s) 90, 228, 411
BspACI CCGC 2 cut(s) 443, 537
BspANI GGCC 1 cut(s) 822
BspCNI CTCAG 1 cut(s) 543
BspFNI CGCG 2 cut(s) 108, 939
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 1 cut(s) 624
BspMAI CTGCAG 1 cut(s) 646
BspPI GGATC 2 cut(s) 85, 236
BspT104I TTCGAA 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 622
BsrDI GCAATG 1 cut(s) 420
BsrGI TGTACA 1 cut(s) 301
BsrI ACTGG 3 cut(s) 333, 639, 823
BssECI CCNNGG 1 cut(s) 753
BssMI GATC 3 cut(s) 90, 228, 411
BssNI GRCGYC 1 cut(s) 860
BssSI CACGAG 1 cut(s) 594
Bst2BI CACGAG 1 cut(s) 594
Bst2UI CCWGG 3 cut(s) 290, 546, 755
Bst4CI ACNGT 2 cut(s) 451, 604
Bst6I CTCTTC 2 cut(s) 342, 514
BstACI GRCGYC 1 cut(s) 860
BstAUI TGTACA 1 cut(s) 301
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 264, 814
BstDEI CTNAG 1 cut(s) 551
BstF5I GGATG 2 cut(s) 228, 643
BstFNI CGCG 2 cut(s) 108, 939
BstHHI GCGC 2 cut(s) 110, 274
BstKTI GATC 3 cut(s) 93, 231, 414
BstMAI GTCTC 4 cut(s) 157, 516, 593, 740
BstMBI GATC 3 cut(s) 90, 228, 411
BstMWI GCNNNNNNNGC 3 cut(s) 650, 673, 813
BstNI CCWGG 3 cut(s) 290, 546, 755
BstNSI RCATGY 1 cut(s) 909
BstSCI CCNGG 3 cut(s) 288, 544, 753
BstSFI CTRYAG 1 cut(s) 642
BstUI CGCG 2 cut(s) 108, 939
BstV1I GCAGC 4 cut(s) 246, 424, 628, 656
BsuRI GGCC 1 cut(s) 822
BtsCI GGATG 2 cut(s) 228, 643
BtsI GCAGTG 1 cut(s) 163
BtsIMutI CAGTG 3 cut(s) 163, 762, 816
Cac8I GCNNGC 2 cut(s) 264, 814
CciI TCATGA 1 cut(s) 738
CfoI GCGC 2 cut(s) 110, 274
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 1 cut(s) 928
Csp6I GTAC 3 cut(s) 302, 773, 850
CviAII CATG 5 cut(s) 739, 824, 882, 906, 919
CviQI GTAC 3 cut(s) 302, 773, 850
DdeI CTNAG 1 cut(s) 551
DpnI GATC 3 cut(s) 92, 230, 413
DpnII GATC 3 cut(s) 90, 228, 411
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 2 cut(s) 342, 514
EarI CTCTTC 2 cut(s) 342, 514
Ecl136II GAGCTC 1 cut(s) 593
Eco24I GRGCYC 1 cut(s) 595
Eco47I GGWCC 1 cut(s) 466
Eco53kI GAGCTC 1 cut(s) 593
Eco57I CTGAAG 2 cut(s) 188, 366
EcoICRI GAGCTC 1 cut(s) 593
EcoRII CCWGG 3 cut(s) 288, 544, 753
EcoT38I GRGCYC 1 cut(s) 595
FaeI CATG 5 cut(s) 742, 827, 885, 909, 922
FatI CATG 5 cut(s) 738, 823, 881, 905, 918
FauI CCCGC 1 cut(s) 544
Fnu4HI GCNGC 4 cut(s) 260, 438, 642, 645
FokI GGATG 2 cut(s) 235, 650
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 4 cut(s) 260, 438, 642, 645
FspBI CTAG 3 cut(s) 17, 341, 714
GlaI GCGC 2 cut(s) 109, 273
GluI GCNGC 4 cut(s) 260, 438, 642, 645
GsaI CCCAGC 1 cut(s) 280
GsuI CTGGAG 2 cut(s) 528, 776
HaeIII GGCC 1 cut(s) 822
HgaI GACGC 1 cut(s) 928
HhaI GCGC 2 cut(s) 110, 274
Hin1I GRCGYC 1 cut(s) 860
Hin1II CATG 5 cut(s) 742, 827, 885, 909, 922
Hin6I GCGC 2 cut(s) 108, 272
HinP1I GCGC 2 cut(s) 108, 272
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 7 cut(s) 113, 176, 504, 532, 614, 745, 779
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 5 cut(s) 406, 514, 531, 552, 891
Hpy188III TCNNGA 6 cut(s) 146, 359, 469, 596, 680, 739
Hpy8I GTNNAC 1 cut(s) 127
HpyAV CCTTC 4 cut(s) 106, 356, 549, 784
HpyCH4III ACNGT 2 cut(s) 451, 604
HpyCH4IV ACGT 1 cut(s) 860
HpyCH4V TGCA 2 cut(s) 266, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 650, 673, 813
HpyF3I CTNAG 1 cut(s) 551
HpySE526I ACGT 1 cut(s) 860
Hsp92I GRCGYC 1 cut(s) 860
Hsp92II CATG 5 cut(s) 742, 827, 885, 909, 922
HspAI GCGC 2 cut(s) 108, 272
Kzo9I GATC 3 cut(s) 90, 228, 411
LmnI GCTCC 2 cut(s) 445, 590
Lsp1109I GCAGC 4 cut(s) 246, 424, 628, 656
LweI GCATC 2 cut(s) 275, 628
MaeI CTAG 3 cut(s) 17, 341, 714
MaeII ACGT 1 cut(s) 860
MaeIII GTNAC 4 cut(s) 52, 395, 746, 940
MalI GATC 3 cut(s) 92, 230, 413
MboI GATC 3 cut(s) 90, 228, 411
MboII GAAGA 6 cut(s) 93, 181, 359, 421, 501, 609
MhlI GDGCHC 1 cut(s) 595
MlsI TGGCCA 1 cut(s) 822
MluCI AATT 5 cut(s) 186, 201, 247, 430, 933
MluNI TGGCCA 1 cut(s) 822
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 2 cut(s) 163, 685
MnlI CCTC 7 cut(s) 219, 235, 246, 249, 579, 583, 676
Mox20I TGGCCA 1 cut(s) 822
MroXI GAANNNNTTC 1 cut(s) 508
MscI TGGCCA 1 cut(s) 822
MseI TTAA 8 cut(s) 44, 71, 204, 474, 606, 630, 932, 946
MslI CAYNNNNRTG 1 cut(s) 920
Msp20I TGGCCA 1 cut(s) 822
MspA1I CMGCKG 1 cut(s) 445
MspR9I CCNGG 3 cut(s) 290, 546, 755
MvaI CCWGG 3 cut(s) 290, 546, 755
MvnI CGCG 2 cut(s) 108, 939
MwoI GCNNNNNNNGC 3 cut(s) 650, 673, 813
NdeII GATC 3 cut(s) 90, 228, 411
NlaIII CATG 5 cut(s) 742, 827, 885, 909, 922
NlaIV GGNNCC 1 cut(s) 624
NmuCI GTSAC 4 cut(s) 52, 395, 746, 940
NspI RCATGY 1 cut(s) 909
NspV TTCGAA 1 cut(s) 116
PagI TCATGA 1 cut(s) 738
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 508
PfeI GAWTC 6 cut(s) 113, 176, 504, 532, 614, 779
PkrI GCNGC 4 cut(s) 261, 439, 643, 646
PleI GAGTC 1 cut(s) 753
PpsI GAGTC 1 cut(s) 753
Psp124BI GAGCTC 1 cut(s) 595
Psp6I CCWGG 3 cut(s) 288, 544, 753
PspFI CCCAGC 1 cut(s) 276
PspGI CCWGG 3 cut(s) 288, 544, 753
PspN4I GGNNCC 1 cut(s) 624
PspPI GGNCC 1 cut(s) 466
PstI CTGCAG 1 cut(s) 646
RsaI GTAC 3 cut(s) 303, 774, 851
RsaNI GTAC 3 cut(s) 302, 773, 850
RseI CAYNNNNRTG 1 cut(s) 920
SacI GAGCTC 1 cut(s) 595
SaqAI TTAA 8 cut(s) 44, 71, 204, 474, 606, 630, 932, 946
SatI GCNGC 4 cut(s) 260, 438, 642, 645
Sau3AI GATC 3 cut(s) 90, 228, 411
Sau96I GGNCC 1 cut(s) 466
ScaI AGTACT 1 cut(s) 774
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 3 cut(s) 290, 546, 755
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 2 cut(s) 275, 628
SfcI CTRYAG 1 cut(s) 642
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 1 cut(s) 466
SmiMI CAYNNNNRTG 1 cut(s) 920
Sse9I AATT 5 cut(s) 186, 201, 247, 430, 933
SsiI CCGC 2 cut(s) 443, 537
SspI AATATT 1 cut(s) 494
SspMI CTAG 3 cut(s) 17, 341, 714
SstI GAGCTC 1 cut(s) 595
StyD4I CCNGG 3 cut(s) 288, 544, 753
TaaI ACNGT 2 cut(s) 451, 604
TaiI ACGT 1 cut(s) 863
TaqI TCGA 4 cut(s) 61, 89, 116, 414
TasI AATT 5 cut(s) 186, 201, 247, 430, 933
TatI WGTACW 3 cut(s) 301, 772, 849
TfiI GAWTC 6 cut(s) 113, 176, 504, 532, 614, 779
Tru1I TTAA 8 cut(s) 44, 71, 204, 474, 606, 630, 932, 946
Tru9I TTAA 8 cut(s) 44, 71, 204, 474, 606, 630, 932, 946
TscAI CASTG 3 cut(s) 163, 769, 823
TseFI GTSAC 4 cut(s) 52, 395, 746, 940
TseI GCWGC 4 cut(s) 259, 437, 641, 644
Tsp45I GTSAC 4 cut(s) 52, 395, 746, 940
TspDTI ATGAA 3 cut(s) 17, 88, 904
TspRI CASTG 3 cut(s) 163, 769, 823
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 2 cut(s) 186, 247
XceI RCATGY 1 cut(s) 909
XmnI GAANNNNTTC 1 cut(s) 508
XspI CTAG 3 cut(s) 17, 341, 714
ZraI GACGTC 1 cut(s) 861
ZrmI AGTACT 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.