pycom16g21130

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004280
Physical Location & Seq
Reverse (-)
19343344 .. 19346551
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
ATGCAGAGAGAAGATTATTGGCAAATGCTCTTCAAGATCGATCCTGATAACCAGCATTTTGTCTTACTTTCAGAAAGTTGTGTACCATTGTACACGTTTGACTATATCTATGACTATCTGATGAATACAAATATAAGCTATGTTGACTGCTTTGAGGATCCTGGTCCGCATGGAAATGGGAGGTATTCTGAACACATGTTACCTGAAATTGAGAAGAAAGACTTTAGAAAGGGAGCACAGTGGTTCTCTATGAAGCGTCAGCATGCTGTGATAGCAATGGCTGATAGTCTCTACTACTCGAAATTCCGGGACTACTGTTTTGACGGGCGCAATTGCATTGCTGATGAACATTACTTGCCAACCTTTTTCAATATAATTGATCCTGGTGGCATTGCAAACTGGTCGGTAACACATGTTGATTGGTCTGAAAGAAAGTGGCATCCAAAGTTATACAAATCTCAGGATATTACATATGAGCTTCTGAAGAATATTACGTCAGTTGATGTGAGCGTGCATGTAACCAGTGATGCGAAGAAAGTAGTACAGAGATGGCCTTGCTTATGGAATGGTATACAACGACCAGGCTTGATTGTTCGCCGCCTCCCTACCACGATTCTTGAGTGCAAGGGCTACTGGCAGAGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

216

Amino Acids

25.5

Weight (kDa)

6.23

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 10 - 164 5.8e-54 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 571
AciI CCGC 2 cut(s) 167, 598
AclWI GGATC 4 cut(s) 35, 152, 165, 374
AcsI RAATTY 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 503
AfaI GTAC 3 cut(s) 84, 92, 543
AflIII ACRYGT 3 cut(s) 93, 195, 412
AgsI TTSAA 2 cut(s) 34, 370
AjnI CCWGG 3 cut(s) 160, 382, 580
AluBI AGCT 2 cut(s) 138, 478
AluI AGCT 2 cut(s) 138, 478
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 4 cut(s) 35, 152, 165, 374
AoxI GGCC 1 cut(s) 551
ApoI RAATTY 1 cut(s) 302
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 308
AvaII GGWCC 1 cut(s) 164
BamHI GGATCC 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 238
BccI CCATC 1 cut(s) 543
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BciT130I CCWGG 3 cut(s) 162, 384, 582
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 293
BisI GCNGC 1 cut(s) 598
BlsI GCNGC 1 cut(s) 599
Bme1390I CCNGG 4 cut(s) 162, 308, 384, 582
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 4 cut(s) 162, 308, 384, 582
BmsI GCATC 2 cut(s) 448, 517
BpuEI CTTGAG 1 cut(s) 638
BpuMI CCSGG 1 cut(s) 308
Bsa29I ATCGAT 1 cut(s) 39
Bse1I ACTGG 3 cut(s) 404, 522, 638
Bse3DI GCAATG 3 cut(s) 282, 336, 390
BseBI CCWGG 3 cut(s) 162, 384, 582
BseCI ATCGAT 1 cut(s) 39
BseGI GGATG 1 cut(s) 439
BseMI GCAATG 3 cut(s) 282, 336, 390
BseMII CTCAG 1 cut(s) 473
BseNI ACTGG 3 cut(s) 404, 522, 638
BshFI GGCC 1 cut(s) 553
BshVI ATCGAT 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 238
BsiSI CCGG 1 cut(s) 307
BslFI GGGAC 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 323
BsnI GGCC 1 cut(s) 553
Bsp1286I GDGCHC 1 cut(s) 238
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 4 cut(s) 36, 40, 157, 379
BspACI CCGC 2 cut(s) 167, 598
BspANI GGCC 1 cut(s) 553
BspCNI CTCAG 1 cut(s) 472
BspDI ATCGAT 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 159
BspPI GGATC 4 cut(s) 35, 152, 165, 374
BspQI GCTCTTC 1 cut(s) 35
BsrDI GCAATG 3 cut(s) 282, 336, 390
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 3 cut(s) 404, 522, 638
BssMI GATC 4 cut(s) 36, 40, 157, 379
BssNAI GTATAC 1 cut(s) 572
Bst1107I GTATAC 1 cut(s) 572
Bst2UI CCWGG 3 cut(s) 162, 384, 582
Bst4CI ACNGT 2 cut(s) 240, 317
Bst6I CTCTTC 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 90
BstC8I GCNNGC 2 cut(s) 264, 512
BstDEI CTNAG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 439
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 4 cut(s) 39, 43, 160, 382
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 4 cut(s) 36, 40, 157, 379
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 3 cut(s) 162, 384, 582
BstNSI RCATGY 4 cut(s) 199, 266, 416, 518
BstSCI CCNGG 4 cut(s) 160, 306, 382, 580
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BstZ17I GTATAC 1 cut(s) 572
Bsu15I ATCGAT 1 cut(s) 39
BsuRI GGCC 1 cut(s) 553
BsuTUI ATCGAT 1 cut(s) 39
BtsCI GGATG 1 cut(s) 439
BtsIMutI CAGTG 2 cut(s) 245, 529
Cac8I GCNNGC 2 cut(s) 264, 512
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 164
ClaI ATCGAT 1 cut(s) 39
CseI GACGC 1 cut(s) 245
Csp6I GTAC 3 cut(s) 83, 91, 542
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviAII CATG 5 cut(s) 170, 196, 263, 413, 515
CviJI RGCY 6 cut(s) 138, 281, 478, 553, 585, 630
CviKI_1 RGCY 6 cut(s) 138, 281, 478, 553, 585, 630
CviQI GTAC 3 cut(s) 83, 91, 542
DdeI CTNAG 1 cut(s) 459
DpnI GATC 4 cut(s) 38, 42, 159, 381
DpnII GATC 4 cut(s) 36, 40, 157, 379
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 164
Eco57I CTGAAG 1 cut(s) 503
EcoRII CCWGG 3 cut(s) 160, 382, 580
FaeI CATG 5 cut(s) 173, 199, 266, 416, 518
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FaqI GGGAC 1 cut(s) 323
FatI CATG 5 cut(s) 169, 195, 262, 412, 514
FauNDI CATATG 1 cut(s) 472
FblI GTMKAC 1 cut(s) 571
Fnu4HI GCNGC 1 cut(s) 598
FokI GGATG 1 cut(s) 426
Fsp4HI GCNGC 1 cut(s) 598
GlaI GCGC 1 cut(s) 329
GluI GCNGC 1 cut(s) 598
HaeIII GGCC 1 cut(s) 553
HapII CCGG 1 cut(s) 307
HgaI GACGC 1 cut(s) 245
HhaI GCGC 1 cut(s) 330
Hin1II CATG 5 cut(s) 173, 199, 266, 416, 518
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 1 cut(s) 613
HpaII CCGG 1 cut(s) 307
Hpy166II GTNNAC 4 cut(s) 83, 93, 145, 572
Hpy188I TCNGA 5 cut(s) 73, 120, 190, 427, 483
Hpy188III TCNNGA 4 cut(s) 34, 44, 461, 617
Hpy8I GTNNAC 4 cut(s) 83, 93, 145, 572
HpyCH4III ACNGT 2 cut(s) 240, 317
HpyCH4IV ACGT 2 cut(s) 95, 494
HpyCH4V TGCA 5 cut(s) 4, 336, 395, 514, 624
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 2 cut(s) 95, 494
Hsp92II CATG 5 cut(s) 173, 199, 266, 416, 518
HspAI GCGC 1 cut(s) 328
Kzo9I GATC 4 cut(s) 36, 40, 157, 379
LguI GCTCTTC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 233
LweI GCATC 2 cut(s) 448, 517
MaeII ACGT 2 cut(s) 95, 494
MaeIII GTNAC 3 cut(s) 198, 406, 517
MalI GATC 4 cut(s) 38, 42, 159, 381
MboI GATC 4 cut(s) 36, 40, 157, 379
MboII GAAGA 5 cut(s) 22, 23, 226, 496, 544
MfeI CAATTG 1 cut(s) 331
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 238
MluCI AATT 4 cut(s) 207, 302, 331, 375
MnlI CCTC 3 cut(s) 148, 174, 611
MslI CAYNNNNRTG 1 cut(s) 174
MspI CCGG 1 cut(s) 307
MspR9I CCNGG 4 cut(s) 162, 308, 384, 582
MunI CAATTG 1 cut(s) 331
MvaI CCWGG 3 cut(s) 162, 384, 582
MwoI GCNNNNNNNGC 1 cut(s) 272
NciI CCSGG 1 cut(s) 308
NdeI CATATG 1 cut(s) 472
NdeII GATC 4 cut(s) 36, 40, 157, 379
NlaIII CATG 5 cut(s) 173, 199, 266, 416, 518
NlaIV GGNNCC 1 cut(s) 159
NspI RCATGY 4 cut(s) 199, 266, 416, 518
PaeI GCATGC 1 cut(s) 266
PciI ACATGT 2 cut(s) 195, 412
PciSI GCTCTTC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 613
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 1 cut(s) 599
PscI ACATGT 2 cut(s) 195, 412
Psp6I CCWGG 3 cut(s) 160, 382, 580
PspGI CCWGG 3 cut(s) 160, 382, 580
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 164
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 3 cut(s) 84, 92, 543
RsaNI GTAC 3 cut(s) 83, 91, 542
RseI CAYNNNNRTG 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 35
SatI GCNGC 1 cut(s) 598
Sau3AI GATC 4 cut(s) 36, 40, 157, 379
Sau96I GGNCC 1 cut(s) 164
ScrFI CCNGG 4 cut(s) 162, 308, 384, 582
SduI GDGCHC 1 cut(s) 238
SetI ASST 7 cut(s) 98, 140, 185, 205, 365, 480, 497
SfaNI GCATC 2 cut(s) 448, 517
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SphI GCATGC 1 cut(s) 266
Sse9I AATT 4 cut(s) 207, 302, 331, 375
SsiI CCGC 2 cut(s) 167, 598
SspI AATATT 1 cut(s) 490
StyD4I CCNGG 4 cut(s) 160, 306, 382, 580
TaaI ACNGT 2 cut(s) 240, 317
TaiI ACGT 2 cut(s) 98, 497
TaqI TCGA 2 cut(s) 39, 299
TasI AATT 4 cut(s) 207, 302, 331, 375
TatI WGTACW 2 cut(s) 90, 541
TauI GCSGC 1 cut(s) 600
TfiI GAWTC 1 cut(s) 613
TscAI CASTG 2 cut(s) 245, 529
TspDTI ATGAA 3 cut(s) 137, 266, 360
TspRI CASTG 2 cut(s) 245, 529
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 302
XceI RCATGY 4 cut(s) 199, 266, 416, 518
XmiI GTMKAC 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.