pycom16g23590

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005070
Physical Location & Seq
Reverse (-)
24733032 .. 24738820
5789 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2478 bp
ATGGCTCAATACAACTTGCATCAGCTCAAGATACCTCATGCAGGATCGGGGCAACACCATCAACCAATTAGTCAGAGGTATAACGAAATGGAAGCTCCTTGGAGTCCTGCATACTATTCTCCTGGGCATCATGGGCACATTGATTCAAGACCAATGGCAGAGTTTCCTTCCTCTCCTTCCTCTGTACGGTACCGTATGCCGTTTCCAGATGTAGCCGATAAATGTTTAGATAGACTGCCTGAAGAATATGCACGACAGCCATTGAATCATCAGCCTCCATATGAACACCTTTCTGAGAATGTTTCATGGCTTCCAAGTGGAGCTATAGGTGGTGAAAAGTCAGGATCTCCAGGTAACGTTTTTCATGGAAACAATGCTATTGAAGGAAACAGTATTTGTGAACACTGCCGGATGGCTTTCCAGAGAAACCAACCATATTTTGAGCAACCAAACATGGCAAATGGGTTTCAGCAGGTTCCTAATCCAGGCAGTGAGGGTACTCCAAATAGAGAGACTTTCATGATGAATCCAGATGCAAAGTTGCACCATGAAATATACGCAAGTGAACAGAATAATGGTCCTCCTCTTTTTAATGAGACTCCAAATCATGAAAGAGGGTGGGCTCAACATCATCGTTTGAATTGTTGGACTGAGGAAACAGGACCACATGCTTCTGGAGCTGGGAAATTGAATGATCCTTACATTGTAGATGGTCCCAACATGAATTTCCCTCTTGGTCCTAATATGGTGGATGGCCATCACATCTCTTCCAATTATGTTCATCACCGAGCTGGACCTGAAGTGGGAAATGAAGTGTTTCACGAACGATCTGTGGCTGCTCTACCCAATGTACACATTGCTCCTGTAGAAGAGTGTGGTGTTCGTTATGGAAATCTTCCTTATGCCTATGGAGGAGATAATATTTATCTGGGGTCTCATGGACATGCAGTATGGAGGAATGCTCAGATCCCAATGCATGCTGCGCCTCCTTATGAAGCATCCGTTTCTGCACCACAAGTGAATGGTTCTGTTAATGTGGGATATTTCAGACGTGGGGATGGTCCAAGGTTTTCCATTGCATTAGACAATCAAAATATTTGGGTTGACTCCTCGCAGAAAATGTTAGGTTTGGAAGGGAAAGCTGTTCCTTCAGACTATTCTTATGGGCACGCTGTGAAGTTGAACCCAAACACACTTTGTCAAGAAAATCATCAAGCATTTCCTCCAGAACCCATTCAGCCCACACCTGATATGATAAATTGTGCTATTCCTTTGAACCCCGTCGCCGGAGTTGTAAGGTTGGAGGAAAAGAGTTCACCTGGAGTAAATGAGGTGAATATTGTGGACAAGGTGGAGAACTCTGAAACAGAGGTTATAAACCCAAACAATCATTGTGATAAAAATGGTGGTGTGGTATCTCTTGAGTCGATAAAGTCAAATTTCAGTAAGCTTGCAGAGGAAAGTGGTAATGCTGGCAAAACAAGTGACGAGCATCAGTCTACTTGTGAACTTTTAAAGCTTTCTGTTAATGATTTGAGTTTCATACCGGAGTTGATTGCTTCTGTTAAAAAAGCTGCACTAGAGGGGGCTGAGGAGGTAAAAGCCAACGTTGAAGAAAGTACTGATCCTGAGAAGAGTAGTTCAATAGGCAAAGAGGCAGCTGCAAAAAATTTGGAACCGAACACTCCTCGAGAAAAGGAATTGGATTCTGATAGTGATAATCTAAAGAATCCCAAAATCGAGCCAACAAAGGCGGAGGCAGAAGCTATTGCCAAGGGATTGCAGACAATAAGGAACGATGATCTAGAGGAGATCCGAGAATTAGGTTCTGGGACTTACGGGGCTGTGTATCATGGGAAGTGGAAGGGGTCTGACGTAGCAATAAAGAGAATAAAATCCAGTTGTTTTGCTGGGAAACCATCAGAAAGAGAACGTTTGATTGCGGATTTCTGGAAGGAGGCTTTGATACTGAGTTCATTGCATCACCCAAATGTTGTTTCCATGTATGGTATTGTGCGCGATGGCCCTGATGGATCTTTAGCAACTGTGACTGAATTCATGTGTGAAAATCTGTTAGTGAATATGAGAGATCCACAGCGGCCTGTATGCAAGATTGGTGATTTGGGATTATCCAAAGTAAAACAACGGACTTTAGTGTCTGGAGGTGTTCGTGGAACTTTACCCTGGATGGCACCTGAGCTTCTTAGCGGGAAAAGTAATATGATTGATGTTTACTCATTTGGGATTGTTATGTGGGAACTGCTTACAGGAGAAGAGCCATATACAGATATGCATTGTGCGTCTATAATTGGAGGTATTGTGAACAACACGTTGCGCCCTCAAATTCCCACGTGGTGCGACCCAGAATGGAAGTCTTTGATGGAAAGTTGTTGGGCTTCTGATCCTTCCCAGAGGCCATCGTTTTCTGAAATTTCTCAGAAGCTAAGAAATATGGCTGCTGCAATGAATGTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

826

Amino Acids

90.84

Weight (kDa)

5.58

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 603 - 690 2.1e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 604 - 693 2e-11 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 689 - 815 1.2e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 689 - 813 9.2e-18 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1376
AasI GACNNNNNNGTC 1 cut(s) 2155
Acc36I ACCTGC 1 cut(s) 463
Acc65I GGTACC 1 cut(s) 189
AccB1I GGYRCC 2 cut(s) 189, 2191
AccI GTMKAC 1 cut(s) 1499
AccII CGCG 1 cut(s) 2019
AciI CCGC 4 cut(s) 1754, 1943, 2098, 2208
AclI AACGTT 3 cut(s) 357, 1608, 1933
AclWI GGATC 9 cut(s) 52, 352, 689, 961, 1619, 1807, 2041, 2084, 2396
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 6 cut(s) 724, 1438, 1669, 2054, 2343, 2430
AcuI CTGAAG 3 cut(s) 261, 819, 1134
AcvI CACGTG 1 cut(s) 2352
AdeI CACNNNGTG 2 cut(s) 1174, 2355
AfaI GTAC 5 cut(s) 186, 191, 499, 852, 1621
AfiI CCNNNNNNNGG 5 cut(s) 41, 186, 485, 803, 1286
AflIII ACRYGT 1 cut(s) 2328
AgsI TTSAA 9 cut(s) 147, 265, 383, 640, 691, 1183, 1276, 1613, 1644
AjiI CACGTC 1 cut(s) 1052
AjnI CCWGG 5 cut(s) 121, 349, 484, 1318, 2183
AjuI GAANNNNNNNTTGG 4 cut(s) 710, 742, 1981, 2013
Alw26I GTCTC 3 cut(s) 506, 590, 939
AlwI GGATC 9 cut(s) 52, 352, 689, 961, 1619, 1807, 2041, 2084, 2396
Ama87I CYCGRG 1 cut(s) 1689
AoxI GGCC 4 cut(s) 754, 2023, 2099, 2414
ApeKI GCWGC 7 cut(s) 836, 980, 1574, 1658, 1661, 2456, 2459
ApoI RAATTY 6 cut(s) 724, 1438, 1669, 2054, 2343, 2430
Asp700I GAANNNNTTC 2 cut(s) 816, 1233
Asp718I GGTACC 1 cut(s) 189
AspLEI GCGC 3 cut(s) 985, 2019, 2337
AspS9I GGNCC 7 cut(s) 578, 662, 713, 737, 794, 1061, 2024
AsuHPI GGTGA 6 cut(s) 344, 776, 1308, 1345, 1976, 2129
AvaI CYCGRG 1 cut(s) 1689
AvaII GGWCC 6 cut(s) 578, 662, 713, 737, 794, 1061
BaeGI GKGCMC 2 cut(s) 138, 1170
BalI TGGCCA 1 cut(s) 756
BanI GGYRCC 2 cut(s) 189, 2191
BanII GRGCYC 1 cut(s) 625
BbrPI CACGTG 1 cut(s) 2352
BbvCI CCTCAGC 1 cut(s) 1590
BbvI GCAGC 7 cut(s) 823, 967, 1561, 1648, 1670, 2443, 2446
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 5 cut(s) 123, 351, 486, 1320, 2185
BcoDI GTCTC 3 cut(s) 506, 590, 939
BfaI CTAG 2 cut(s) 1580, 1805
BfmI CTRYAG 2 cut(s) 324, 864
BfuAI ACCTGC 1 cut(s) 463
BisI GCNGC 8 cut(s) 837, 981, 1575, 1659, 1662, 2099, 2457, 2460
BlsI GCNGC 8 cut(s) 838, 982, 1576, 1660, 1663, 2100, 2458, 2461
BmcAI AGTACT 1 cut(s) 1621
Bme1390I CCNGG 5 cut(s) 123, 351, 486, 1320, 2185
Bme18I GGWCC 6 cut(s) 578, 662, 713, 737, 794, 1061
BmeT110I CYCGRG 1 cut(s) 1689
BmgBI CACGTC 1 cut(s) 1052
BmgT120I GGNCC 7 cut(s) 578, 662, 713, 737, 794, 1061, 2024
BmiI GGNNCC 5 cut(s) 191, 477, 715, 1677, 2193
BmrFI CCNGG 5 cut(s) 123, 351, 486, 1320, 2185
BmsI GCATC 6 cut(s) 28, 136, 523, 1007, 1501, 1990
BplI GAGNNNNNCTC 2 cut(s) 946, 978
BpmI CTGGAG 5 cut(s) 333, 696, 1209, 1341, 2181
Bpu10I CCTNAGC 2 cut(s) 1590, 2196
BpuEI CTTGAG 2 cut(s) 11, 1442
BsaAI YACGTR 1 cut(s) 2352
BsaBI GATNNNNATC 1 cut(s) 756
BsaI GGTCTC 1 cut(s) 939
BsaJI CCNNGG 5 cut(s) 98, 122, 1064, 1773, 2183
BsaWI WCCGGW 1 cut(s) 1546
BsaXI ACNNNNNCTCC 2 cut(s) 2304, 2334
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 186, 485, 803, 1286
Bse1I ACTGG 1 cut(s) 1899
Bse3DI GCAATG 4 cut(s) 855, 1074, 1976, 2469
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 5 cut(s) 123, 351, 486, 1320, 2185
BseDI CCNNGG 5 cut(s) 98, 122, 1064, 1773, 2183
BseGI GGATG 5 cut(s) 417, 757, 998, 1063, 2193
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 5 cut(s) 41, 186, 485, 803, 1286
BseMI GCAATG 4 cut(s) 855, 1074, 1976, 2469
BseMII CTCAG 8 cut(s) 285, 642, 977, 1581, 1620, 1961, 2187, 2450
BseNI ACTGG 1 cut(s) 1899
BseRI GAGGAG 6 cut(s) 573, 927, 1099, 1607, 1677, 1823
BseSI GKGCMC 2 cut(s) 138, 1170
BseXI GCAGC 7 cut(s) 823, 967, 1561, 1648, 1670, 2443, 2446
BseYI CCCAGC 2 cut(s) 680, 1910
BsgI GTGCAG 2 cut(s) 993, 1560
Bsh1236I CGCG 1 cut(s) 2019
BshFI GGCC 4 cut(s) 756, 2025, 2101, 2416
BshNI GGYRCC 2 cut(s) 189, 2191
BsiHKCI CYCGRG 1 cut(s) 1689
BsiSI CCGG 3 cut(s) 409, 1287, 1547
BslFI GGGAC 2 cut(s) 699, 1846
BslI CCNNNNNNNGG 5 cut(s) 41, 186, 485, 803, 1286
BsmAI GTCTC 3 cut(s) 506, 590, 939
BsmFI GGGAC 2 cut(s) 699, 1846
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 4 cut(s) 756, 2025, 2101, 2416
Bso31I GGTCTC 1 cut(s) 939
BsoBI CYCGRG 1 cut(s) 1689
Bsp1286I GDGCHC 3 cut(s) 138, 625, 1170
Bsp1407I TGTACA 1 cut(s) 850
BspACI CCGC 4 cut(s) 1754, 1943, 2098, 2208
BspANI GGCC 4 cut(s) 756, 2025, 2101, 2416
BspCNI CTCAG 8 cut(s) 286, 643, 976, 1582, 1621, 1962, 2188, 2449
BspFNI CGCG 1 cut(s) 2019
BspHI TCATGA 2 cut(s) 519, 607
BspLI GGNNCC 5 cut(s) 191, 477, 715, 1677, 2193
BspMI ACCTGC 1 cut(s) 463
BspPI GGATC 9 cut(s) 52, 352, 689, 961, 1619, 1807, 2041, 2084, 2396
BspQI GCTCTTC 1 cut(s) 2268
BspT107I GGYRCC 2 cut(s) 189, 2191
BspTNI GGTCTC 1 cut(s) 939
BsrDI GCAATG 4 cut(s) 855, 1074, 1976, 2469
BsrGI TGTACA 1 cut(s) 850
BsrI ACTGG 1 cut(s) 1899
BssECI CCNNGG 5 cut(s) 98, 122, 1064, 1773, 2183
BssT1I CCWWGG 3 cut(s) 98, 1064, 1773
Bst2UI CCWGG 5 cut(s) 123, 351, 486, 1320, 2185
Bst4CI ACNGT 4 cut(s) 189, 194, 392, 2047
Bst6I CTCTTC 4 cut(s) 772, 864, 1628, 2268
BstAUI TGTACA 1 cut(s) 850
BstBAI YACGTR 1 cut(s) 2352
BstC8I GCNNGC 4 cut(s) 978, 1170, 1452, 1474
BstENI CCTNNNNNAGG 2 cut(s) 39, 483
BstF5I GGATG 5 cut(s) 417, 757, 998, 1063, 2193
BstFNI CGCG 1 cut(s) 2019
BstHHI GCGC 3 cut(s) 985, 2019, 2337
BstMAI GTCTC 3 cut(s) 506, 590, 939
BstMWI GCNNNNNNNGC 3 cut(s) 133, 677, 982
BstNI CCWGG 5 cut(s) 123, 351, 486, 1320, 2185
BstNSI RCATGY 3 cut(s) 671, 947, 980
BstSCI CCNGG 5 cut(s) 121, 349, 484, 1318, 2183
BstSFI CTRYAG 2 cut(s) 324, 864
BstSLI GKGCMC 2 cut(s) 138, 1170
BstUI CGCG 1 cut(s) 2019
BstV1I GCAGC 7 cut(s) 823, 967, 1561, 1648, 1670, 2443, 2446
BstX2I RGATCY 5 cut(s) 344, 966, 1812, 2033, 2089
BstYI RGATCY 5 cut(s) 344, 966, 1812, 2033, 2089
BsuRI GGCC 4 cut(s) 756, 2025, 2101, 2416
BtgZI GCGATG 1 cut(s) 2034
BtrI CACGTC 1 cut(s) 1052
BtsCI GGATG 5 cut(s) 417, 757, 998, 1063, 2193
BtsI GCAGTG 2 cut(s) 403, 496
BtsIMutI CAGTG 2 cut(s) 403, 496
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 4 cut(s) 978, 1170, 1452, 1474
CciI TCATGA 2 cut(s) 519, 607
CfoI GCGC 3 cut(s) 985, 2019, 2337
Cfr13I GGNCC 7 cut(s) 578, 662, 713, 737, 794, 1061, 2024
CseI GACGC 1 cut(s) 2289
Csp6I GTAC 5 cut(s) 185, 190, 498, 851, 1620
CviQI GTAC 5 cut(s) 185, 190, 498, 851, 1620
DraI TTTAAA 1 cut(s) 1515
DraIII CACNNNGTG 2 cut(s) 1174, 2355
DrdI GACNNNNNNGTC 1 cut(s) 2155
DseDI GACNNNNNNGTC 1 cut(s) 2155
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 4 cut(s) 772, 864, 1628, 2268
EarI CTCTTC 4 cut(s) 772, 864, 1628, 2268
EciI GGCGGA 1 cut(s) 1769
Eco130I CCWWGG 3 cut(s) 98, 1064, 1773
Eco24I GRGCYC 1 cut(s) 625
Eco31I GGTCTC 1 cut(s) 939
Eco47I GGWCC 6 cut(s) 578, 662, 713, 737, 794, 1061
Eco57I CTGAAG 3 cut(s) 261, 819, 1134
Eco72I CACGTG 1 cut(s) 2352
Eco88I CYCGRG 1 cut(s) 1689
EcoNI CCTNNNNNAGG 2 cut(s) 39, 483
EcoRI GAATTC 1 cut(s) 2054
EcoRII CCWGG 5 cut(s) 121, 349, 484, 1318, 2183
EcoT14I CCWWGG 3 cut(s) 98, 1064, 1773
EcoT22I ATGCAT 2 cut(s) 978, 2295
EcoT38I GRGCYC 1 cut(s) 625
ErhI CCWWGG 3 cut(s) 98, 1064, 1773
FaqI GGGAC 2 cut(s) 699, 1846
FauI CCCGC 1 cut(s) 2201
FauNDI CATATG 1 cut(s) 280
FblI GTMKAC 1 cut(s) 1499
Fnu4HI GCNGC 8 cut(s) 837, 981, 1575, 1659, 1662, 2099, 2457, 2460
FokI GGATG 5 cut(s) 424, 764, 985, 1070, 2200
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 8 cut(s) 837, 981, 1575, 1659, 1662, 2099, 2457, 2460
FspBI CTAG 2 cut(s) 1580, 1805
GlaI GCGC 3 cut(s) 984, 2018, 2336
GluI GCNGC 8 cut(s) 837, 981, 1575, 1659, 1662, 2099, 2457, 2460
GsaI CCCAGC 2 cut(s) 684, 1914
GsuI CTGGAG 5 cut(s) 333, 696, 1209, 1341, 2181
HaeIII GGCC 4 cut(s) 756, 2025, 2101, 2416
HapII CCGG 3 cut(s) 409, 1287, 1547
HgaI GACGC 1 cut(s) 2289
HhaI GCGC 3 cut(s) 985, 2019, 2337
Hin6I GCGC 3 cut(s) 983, 2017, 2335
HinP1I GCGC 3 cut(s) 983, 2017, 2335
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HindIII AAGCTT 2 cut(s) 1448, 1517
HinfI GANTC 9 cut(s) 103, 143, 265, 526, 598, 1106, 1424, 1706, 1729
HpaII CCGG 3 cut(s) 409, 1287, 1547
HphI GGTGA 6 cut(s) 344, 776, 1308, 1345, 1976, 2129
Hpy99I CGWCG 1 cut(s) 1286
HpyAV CCTTC 8 cut(s) 177, 186, 377, 1127, 1158, 1858, 1948, 2415
HpyCH4III ACNGT 4 cut(s) 189, 194, 392, 2047
HpyCH4IV ACGT 7 cut(s) 357, 1051, 1608, 1875, 1933, 2330, 2351
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 677, 982
HpySE526I ACGT 7 cut(s) 357, 1051, 1608, 1875, 1933, 2330, 2351
HspAI GCGC 3 cut(s) 983, 2017, 2335
KpnI GGTACC 1 cut(s) 193
LguI GCTCTTC 1 cut(s) 2268
LmnI GCTCC 4 cut(s) 100, 320, 677, 865
Lsp1109I GCAGC 7 cut(s) 823, 967, 1561, 1648, 1670, 2443, 2446
LweI GCATC 6 cut(s) 28, 136, 523, 1007, 1501, 1990
MaeI CTAG 2 cut(s) 1580, 1805
MaeII ACGT 7 cut(s) 357, 1051, 1608, 1875, 1933, 2330, 2351
MaeIII GTNAC 3 cut(s) 353, 1484, 2047
MboII GAAGA 7 cut(s) 254, 759, 881, 887, 1625, 1645, 2285
MflI RGATCY 5 cut(s) 344, 966, 1812, 2033, 2089
MhlI GDGCHC 3 cut(s) 138, 625, 1170
MlsI TGGCCA 1 cut(s) 756
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 4 cut(s) 112, 592, 1100, 1433
MmeI TCCRAC 2 cut(s) 626, 1281
Mox20I TGGCCA 1 cut(s) 756
Mph1103I ATGCAT 2 cut(s) 978, 2295
MroXI GAANNNNTTC 2 cut(s) 816, 1233
MscI TGGCCA 1 cut(s) 756
MseI TTAA 5 cut(s) 591, 1032, 1514, 1527, 1566
MslI CAYNNNNRTG 2 cut(s) 942, 1989
Msp20I TGGCCA 1 cut(s) 756
MspA1I CMGCKG 2 cut(s) 1661, 2098
MspI CCGG 3 cut(s) 409, 1287, 1547
MspR9I CCNGG 5 cut(s) 123, 351, 486, 1320, 2185
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 5 cut(s) 123, 351, 486, 1320, 2185
MvnI CGCG 1 cut(s) 2019
MwoI GCNNNNNNNGC 3 cut(s) 133, 677, 982
NdeI CATATG 1 cut(s) 280
NlaIV GGNNCC 5 cut(s) 191, 477, 715, 1677, 2193
NmuCI GTSAC 2 cut(s) 1484, 2047
NsiI ATGCAT 2 cut(s) 978, 2295
NspI RCATGY 3 cut(s) 671, 947, 980
PaeI GCATGC 1 cut(s) 980
PaeR7I CTCGAG 1 cut(s) 1689
PagI TCATGA 2 cut(s) 519, 607
PciSI GCTCTTC 1 cut(s) 2268
PctI GAATGC 1 cut(s) 964
PdmI GAANNNNTTC 2 cut(s) 816, 1233
PfeI GAWTC 5 cut(s) 143, 265, 526, 1706, 1729
PkrI GCNGC 8 cut(s) 838, 982, 1576, 1660, 1663, 2100, 2458, 2461
PleI GAGTC 4 cut(s) 111, 592, 1100, 1432
PmaCI CACGTG 1 cut(s) 2352
PmlI CACGTG 1 cut(s) 2352
PpsI GAGTC 4 cut(s) 111, 592, 1100, 1432
Ppu21I YACGTR 1 cut(s) 2352
PsiI TTATAA 1 cut(s) 1376
Psp1406I AACGTT 3 cut(s) 357, 1608, 1933
Psp6I CCWGG 5 cut(s) 121, 349, 484, 1318, 2183
PspCI CACGTG 1 cut(s) 2352
PspFI CCCAGC 2 cut(s) 680, 1910
PspGI CCWGG 5 cut(s) 121, 349, 484, 1318, 2183
PspN4I GGNNCC 5 cut(s) 191, 477, 715, 1677, 2193
PspPI GGNCC 7 cut(s) 578, 662, 713, 737, 794, 1061, 2024
PsuI RGATCY 5 cut(s) 344, 966, 1812, 2033, 2089
PvuII CAGCTG 1 cut(s) 1661
RsaI GTAC 5 cut(s) 186, 191, 499, 852, 1621
RsaNI GTAC 5 cut(s) 185, 190, 498, 851, 1620
RseI CAYNNNNRTG 2 cut(s) 942, 1989
SapI GCTCTTC 1 cut(s) 2268
SaqAI TTAA 5 cut(s) 591, 1032, 1514, 1527, 1566
SatI GCNGC 8 cut(s) 837, 981, 1575, 1659, 1662, 2099, 2457, 2460
Sau96I GGNCC 7 cut(s) 578, 662, 713, 737, 794, 1061, 2024
ScaI AGTACT 1 cut(s) 1621
SchI GAGTC 4 cut(s) 112, 592, 1100, 1433
ScrFI CCNGG 5 cut(s) 123, 351, 486, 1320, 2185
SduI GDGCHC 3 cut(s) 138, 625, 1170
SfaNI GCATC 6 cut(s) 28, 136, 523, 1007, 1501, 1990
SfcI CTRYAG 2 cut(s) 324, 864
Sfr274I CTCGAG 1 cut(s) 1689
SinI GGWCC 6 cut(s) 578, 662, 713, 737, 794, 1061
SlaI CTCGAG 1 cut(s) 1689
SmiMI CAYNNNNRTG 2 cut(s) 942, 1989
SmlI CTYRAG 3 cut(s) 26, 1421, 1689
SmoI CTYRAG 3 cut(s) 26, 1421, 1689
SphI GCATGC 1 cut(s) 980
SsiI CCGC 4 cut(s) 1754, 1943, 2098, 2208
SspI AATATT 3 cut(s) 922, 1096, 1339
SspMI CTAG 2 cut(s) 1580, 1805
StyD4I CCNGG 5 cut(s) 121, 349, 484, 1318, 2183
StyI CCWWGG 3 cut(s) 98, 1064, 1773
TaaI ACNGT 4 cut(s) 189, 194, 392, 2047
TaiI ACGT 7 cut(s) 360, 1054, 1611, 1878, 1936, 2333, 2354
TaqI TCGA 3 cut(s) 1427, 1690, 1740
TatI WGTACW 2 cut(s) 850, 1619
TauI GCSGC 1 cut(s) 2101
TfiI GAWTC 5 cut(s) 143, 265, 526, 1706, 1729
Tru1I TTAA 5 cut(s) 591, 1032, 1514, 1527, 1566
Tru9I TTAA 5 cut(s) 591, 1032, 1514, 1527, 1566
TscAI CASTG 2 cut(s) 410, 496
TseFI GTSAC 2 cut(s) 1484, 2047
TseI GCWGC 7 cut(s) 836, 980, 1574, 1658, 1661, 2456, 2459
Tsp45I GTSAC 2 cut(s) 1484, 2047
TspGWI ACGGA 2 cut(s) 991, 2161
TspRI CASTG 2 cut(s) 410, 496
VpaK11BI GGWCC 6 cut(s) 578, 662, 713, 737, 794, 1061
XagI CCTNNNNNAGG 2 cut(s) 39, 483
XapI RAATTY 6 cut(s) 724, 1438, 1669, 2054, 2343, 2430
XbaI TCTAGA 1 cut(s) 1804
XceI RCATGY 3 cut(s) 671, 947, 980
XhoI CTCGAG 1 cut(s) 1689
XmiI GTMKAC 1 cut(s) 1499
XmnI GAANNNNTTC 2 cut(s) 816, 1233
XspI CTAG 2 cut(s) 1580, 1805
ZrmI AGTACT 1 cut(s) 1621
Zsp2I ATGCAT 2 cut(s) 978, 2295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.