pycom17g01460

HAUS augmin-like complex subunit 6 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002442
Physical Location & Seq
Forward (+)
994575 .. 998243
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1467 bp
ATGACAATGGACAGAGAGAAGGAGAGAGAGATAGAGCTAGAGAGTGCAATGTACACTAACTGTTTGCTGTTAGGGTTGGATCCGGCGATTATCGGGCTCGGAGGCTCCAATGCCACTCCTCGGGTCGGGTTCTTCCGCCACTCCAACCCTAAATTGGGCGAACAGCTCCTCTACTTCATCCTCTCTTCTCTCAGAGGCCCAATTCAATCCGCCAAGGATTTCGATAAGGTCTGGCCAATCTTCGATTCCGCGCAATCGCGGGATTTTAGAAAGGTTGTGCAAGGGATAATTAGCGAGCTCGAATCGCAAGGGGCGCTTCCGAGGAGCAATTCGAGGGTTTCATCACTCGCTACATGTTGCGGACCCAGGTTTGTGGAACTTCTGTGGCAACTTTCTTTGCATGCTTTGCGAGAGGTTCATAGGAGAACATTTGCAGCTGATGTAGCTTCTAACCCACTTCCTGCATCACTGACTGATGTAGCTTTTTCACACGCAGCCACTTTACTTCCTGTCACGAAGGCTAGAATAGCACTTGAACGAAGAAGATTTCTTAAGAATGCGGAAACAGCAGTACAGAGACAGGCCATGTGGTCGAATTTGGCTCATGAAATGACTGCAGAGTTTCGTGGTCTTTGTGCTGAAGAGGCTTATTTACAGCAAGAACTGGAAAAATTACATGACCTTAGGAACAAAGTGAAGTTGGAAGGGGAACACTGGGATGACCTTGTTTCTAGTTCGAGTCAGAATTCCCATTTAGTATCAAAAGCTACTCGTTTGTGGGAGTCAATATTAGCCCGTAAAAGTCAACATGAAGTTCTTGCTTCAGGGCCTATTGAGGATTTAATAGCTCACAGGGAGCATAGGTACCGCATCTCTGGATCGTCTTTGCTTGCAGCTATGGATCAGAGTTCTCAGGTTCCTTATGGAGATGTCTTATCTGTCCAGTCTGGTGACTTTACTCCAACACATGTGGATGGCAAGGAACAAAATGATGGAGCAGATTCATCCCATTCACAAGTAAACGATGAAGCACTTCATCGAGCAGATGAAAGAAGTGGAAGAGTCCACCCAACCGTTGACGTGGCAGAAATTATCAGGCGTTGGACGCATGCTTTGCAGCGTATTCATAAACAATCGCTGCACATGGCAAAGGTTAACGAGGGAGAGGGTCCAGAAATTTTAAGAAGTGCACATGATGGCAGTTCAAGTGGTCATACAGAGTCTTTAGCTGCAACTCTTGCTGAACATCAGCAACACTTGGTTAGCTTTCAGGTTCTCATTAACCAACTGAAGGAAGTTGCTCCGGCAATACAAAAGTCAATATCAGAATGTACAGATAAAGTAGATAGCATTTCATCTAGTCTGCCGTCCGATCGATCAAACAACCTGGTCAATCAACTTCACCTATTCAAGCACAGAGTAGTGGAAGGACATTGGAAAGTAACACTGATGATGTGGCTGAGGTGA

Protein Analysis

489

Amino Acids

54.61

Weight (kDa)

6.5

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS6_N PF14661 17 - 261 7e-44 HAUS augmin-like complex subunit 6 N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 864
AccB1I GGYRCC 1 cut(s) 864
AccII CGCG 2 cut(s) 251, 259
AciI CCGC 7 cut(s) 136, 210, 249, 259, 360, 560, 868
AclWI GGATC 4 cut(s) 74, 87, 886, 909
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 3 cut(s) 595, 745, 1176
AcuI CTGAAG 3 cut(s) 660, 807, 1310
AfaI GTAC 4 cut(s) 53, 573, 866, 1333
AfiI CCNNNNNNNGG 5 cut(s) 120, 125, 154, 155, 1291
AflII CTTAAG 1 cut(s) 551
AflIII ACRYGT 2 cut(s) 353, 967
AgsI TTSAA 4 cut(s) 206, 536, 1206, 1411
AjiI CACGTC 1 cut(s) 1081
AjnI CCWGG 2 cut(s) 365, 1386
AjuI GAANNNNNNNTTGG 2 cut(s) 229, 261
Alw21I GWGCWC 2 cut(s) 300, 1192
Alw26I GTCTC 1 cut(s) 571
Alw44I GTGCAC 1 cut(s) 1188
AlwI GGATC 4 cut(s) 74, 87, 886, 909
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 4 cut(s) 196, 233, 582, 827
ApaLI GTGCAC 1 cut(s) 1188
ApeKI GCWGC 6 cut(s) 434, 494, 893, 1117, 1138, 1229
ApoI RAATTY 3 cut(s) 595, 745, 1176
ArsI GACNNNNNNTTYG 2 cut(s) 1096, 1128
Asp700I GAANNNNTTC 1 cut(s) 1032
Asp718I GGTACC 1 cut(s) 864
AspLEI GCGC 2 cut(s) 253, 316
AspS9I GGNCC 4 cut(s) 197, 362, 827, 1169
AsuHPI GGTGA 2 cut(s) 962, 1394
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 2 cut(s) 362, 1169
AxyI CCTNAGG 1 cut(s) 683
BaeGI GKGCMC 1 cut(s) 1192
BalI TGGCCA 1 cut(s) 235
BamHI GGATCC 1 cut(s) 79
BanI GGYRCC 1 cut(s) 864
BanII GRGCYC 2 cut(s) 99, 300
Bbv12I GWGCWC 2 cut(s) 300, 1192
BbvCI CCTCAGC 1 cut(s) 1460
BbvI GCAGC 6 cut(s) 446, 506, 905, 1125, 1129, 1216
BccI CCATC 3 cut(s) 968, 986, 1190
BceAI ACGGC 1 cut(s) 1351
BciT130I CCWGG 2 cut(s) 367, 1388
BcoDI GTCTC 1 cut(s) 571
BfaI CTAG 4 cut(s) 38, 522, 732, 1359
BfmI CTRYAG 1 cut(s) 615
BfoI RGCGCY 1 cut(s) 317
BfrI CTTAAG 1 cut(s) 551
BisI GCNGC 6 cut(s) 435, 495, 894, 1118, 1139, 1230
BlsI GCNGC 6 cut(s) 436, 496, 895, 1119, 1140, 1231
Bme1390I CCNGG 2 cut(s) 367, 1388
Bme18I GGWCC 2 cut(s) 362, 1169
BmeT110I CYCGRG 1 cut(s) 120
BmgBI CACGTC 1 cut(s) 1081
BmgT120I GGNCC 4 cut(s) 197, 362, 827, 1169
BmiI GGNNCC 6 cut(s) 81, 106, 364, 866, 918, 1170
BmrFI CCNGG 2 cut(s) 367, 1388
BmrI ACTGGG 1 cut(s) 724
BmsI GCATC 2 cut(s) 473, 879
BmuI ACTGGG 1 cut(s) 724
Bpu10I CCTNAGC 1 cut(s) 1460
Bsa29I ATCGAT 1 cut(s) 1375
BsaJI CCNNGG 4 cut(s) 119, 213, 320, 365
BsaXI ACNNNNNCTCC 2 cut(s) 848, 878
Bsc4I CCNNNNNNNGG 5 cut(s) 120, 125, 154, 155, 1291
Bse1I ACTGG 3 cut(s) 669, 719, 943
Bse21I CCTNAGG 1 cut(s) 683
Bse3DI GCAATG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 367, 1388
BseCI ATCGAT 1 cut(s) 1375
BseDI CCNNGG 4 cut(s) 119, 213, 320, 365
BseGI GGATG 4 cut(s) 177, 724, 979, 1004
BseLI CCNNNNNNNGG 5 cut(s) 120, 125, 154, 155, 1291
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 3 cut(s) 205, 926, 1451
BseNI ACTGG 3 cut(s) 669, 719, 943
BseRI GAGGAG 3 cut(s) 108, 158, 337
BseSI GKGCMC 1 cut(s) 1192
BseXI GCAGC 6 cut(s) 446, 506, 905, 1125, 1129, 1216
BsgI GTGCAG 1 cut(s) 1124
Bsh1236I CGCG 2 cut(s) 251, 259
Bsh1285I CGRYCG 1 cut(s) 1375
BshFI GGCC 4 cut(s) 198, 235, 584, 829
BshNI GGYRCC 1 cut(s) 864
BshVI ATCGAT 1 cut(s) 1375
BsiEI CGRYCG 1 cut(s) 1375
BsiHKAI GWGCWC 2 cut(s) 300, 1192
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 2 cut(s) 83, 1304
BslI CCNNNNNNNGG 5 cut(s) 120, 125, 154, 155, 1291
BsmAI GTCTC 1 cut(s) 571
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 4 cut(s) 198, 235, 584, 829
BsoBI CYCGRG 1 cut(s) 120
Bsp1286I GDGCHC 3 cut(s) 99, 300, 1192
Bsp1407I TGTACA 2 cut(s) 51, 1331
Bsp143I GATC 5 cut(s) 79, 878, 901, 1372, 1376
BspACI CCGC 7 cut(s) 136, 210, 249, 259, 360, 560, 868
BspANI GGCC 4 cut(s) 198, 235, 584, 829
BspCNI CTCAG 3 cut(s) 204, 925, 1452
BspDI ATCGAT 1 cut(s) 1375
BspFNI CGCG 2 cut(s) 251, 259
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 6 cut(s) 81, 106, 364, 866, 918, 1170
BspMAI CTGCAG 1 cut(s) 619
BspPI GGATC 4 cut(s) 74, 87, 886, 909
BspT107I GGYRCC 1 cut(s) 864
BspTI CTTAAG 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 54
BsrGI TGTACA 2 cut(s) 51, 1331
BsrI ACTGG 3 cut(s) 669, 719, 943
BssECI CCNNGG 4 cut(s) 119, 213, 320, 365
BssMI GATC 5 cut(s) 79, 878, 901, 1372, 1376
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 2 cut(s) 367, 1388
Bst4CI ACNGT 2 cut(s) 62, 1075
Bst6I CTCTTC 3 cut(s) 190, 636, 1054
BstAFI CTTAAG 1 cut(s) 551
BstAPI GCANNNNNTGC 3 cut(s) 406, 1114, 1238
BstAUI TGTACA 2 cut(s) 51, 1331
BstC8I GCNNGC 4 cut(s) 296, 402, 891, 1110
BstDEI CTNAG 4 cut(s) 191, 683, 912, 1460
BstF5I GGATG 4 cut(s) 177, 724, 979, 1004
BstFNI CGCG 2 cut(s) 251, 259
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 2 cut(s) 253, 316
BstKTI GATC 5 cut(s) 82, 881, 904, 1375, 1379
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 5 cut(s) 79, 878, 901, 1372, 1376
BstMCI CGRYCG 1 cut(s) 1375
BstNI CCWGG 2 cut(s) 367, 1388
BstNSI RCATGY 4 cut(s) 357, 404, 971, 1112
BstSCI CCNGG 2 cut(s) 365, 1386
BstSFI CTRYAG 1 cut(s) 615
BstSLI GKGCMC 1 cut(s) 1192
BstUI CGCG 2 cut(s) 251, 259
BstV1I GCAGC 6 cut(s) 446, 506, 905, 1125, 1129, 1216
BstX2I RGATCY 1 cut(s) 79
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 1 cut(s) 79
Bsu15I ATCGAT 1 cut(s) 1375
Bsu36I CCTNAGG 1 cut(s) 683
BsuRI GGCC 4 cut(s) 198, 235, 584, 829
BsuTUI ATCGAT 1 cut(s) 1375
BtrI CACGTC 1 cut(s) 1081
BtsCI GGATG 4 cut(s) 177, 724, 979, 1004
BtsIMutI CAGTG 3 cut(s) 467, 712, 1445
Cac8I GCNNGC 4 cut(s) 296, 402, 891, 1110
CciI TCATGA 1 cut(s) 604
CfoI GCGC 2 cut(s) 253, 316
Cfr13I GGNCC 4 cut(s) 197, 362, 827, 1169
ClaI ATCGAT 1 cut(s) 1375
CseI GACGC 1 cut(s) 1114
CsiI ACCWGGT 1 cut(s) 1386
Csp6I GTAC 4 cut(s) 52, 572, 865, 1332
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
CviQI GTAC 4 cut(s) 52, 572, 865, 1332
DdeI CTNAG 4 cut(s) 191, 683, 912, 1460
DpnI GATC 5 cut(s) 81, 880, 903, 1374, 1378
DpnII GATC 5 cut(s) 79, 878, 901, 1372, 1376
EaeI YGGCCR 1 cut(s) 233
Eam1104I CTCTTC 3 cut(s) 190, 636, 1054
EarI CTCTTC 3 cut(s) 190, 636, 1054
EciI GGCGGA 2 cut(s) 125, 199
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 2 cut(s) 99, 300
Eco47I GGWCC 2 cut(s) 362, 1169
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 3 cut(s) 660, 807, 1310
Eco81I CCTNAGG 1 cut(s) 683
Eco88I CYCGRG 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 827
EcoRI GAATTC 1 cut(s) 745
EcoRII CCWGG 2 cut(s) 365, 1386
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 2 cut(s) 99, 300
ErhI CCWWGG 1 cut(s) 213
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FauI CCCGC 1 cut(s) 252
Fnu4HI GCNGC 6 cut(s) 435, 495, 894, 1118, 1139, 1230
FokI GGATG 4 cut(s) 164, 731, 986, 991
FriOI GRGCYC 2 cut(s) 99, 300
Fsp4HI GCNGC 6 cut(s) 435, 495, 894, 1118, 1139, 1230
FspBI CTAG 4 cut(s) 38, 522, 732, 1359
GlaI GCGC 2 cut(s) 252, 315
GluI GCNGC 6 cut(s) 435, 495, 894, 1118, 1139, 1230
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 4 cut(s) 198, 235, 584, 829
HapII CCGG 2 cut(s) 83, 1304
HgaI GACGC 1 cut(s) 1114
HhaI GCGC 2 cut(s) 253, 316
Hin6I GCGC 2 cut(s) 251, 314
HinP1I GCGC 2 cut(s) 251, 314
HincII GTYRAC 3 cut(s) 806, 1078, 1156
HindII GTYRAC 3 cut(s) 806, 1078, 1156
HinfI GANTC 7 cut(s) 245, 302, 739, 782, 1001, 1062, 1220
HpaI GTTAAC 1 cut(s) 1156
HpaII CCGG 2 cut(s) 83, 1304
HphI GGTGA 2 cut(s) 962, 1394
Hpy166II GTNNAC 7 cut(s) 54, 806, 1021, 1066, 1078, 1156, 1190
Hpy188I TCNGA 7 cut(s) 101, 194, 321, 744, 906, 1327, 1372
Hpy188III TCNNGA 4 cut(s) 514, 605, 876, 1172
Hpy8I GTNNAC 7 cut(s) 54, 806, 1021, 1066, 1078, 1156, 1190
HpyAV CCTTC 5 cut(s) 13, 511, 698, 1285, 1421
HpyCH4III ACNGT 2 cut(s) 62, 1075
HpyCH4IV ACGT 1 cut(s) 1080
HpyF3I CTNAG 4 cut(s) 191, 683, 912, 1460
HpySE526I ACGT 1 cut(s) 1080
HspAI GCGC 2 cut(s) 251, 314
KpnI GGTACC 1 cut(s) 868
KspAI GTTAAC 1 cut(s) 1156
Kzo9I GATC 5 cut(s) 79, 878, 901, 1372, 1376
LmnI GCTCC 6 cut(s) 110, 171, 324, 856, 995, 1306
Lsp1109I GCAGC 6 cut(s) 446, 506, 905, 1125, 1129, 1216
LweI GCATC 2 cut(s) 473, 879
MabI ACCWGGT 1 cut(s) 1386
MaeI CTAG 4 cut(s) 38, 522, 732, 1359
MaeII ACGT 1 cut(s) 1080
MaeIII GTNAC 3 cut(s) 511, 950, 1441
MalI GATC 5 cut(s) 81, 880, 903, 1374, 1378
MboI GATC 5 cut(s) 79, 878, 901, 1372, 1376
MboII GAAGA 7 cut(s) 124, 177, 232, 552, 555, 653, 1071
MflI RGATCY 1 cut(s) 79
MhlI GDGCHC 3 cut(s) 99, 300, 1192
MlsI TGGCCA 1 cut(s) 235
MluCI AATT 9 cut(s) 152, 201, 288, 328, 595, 671, 745, 1089, 1176
MluNI TGGCCA 1 cut(s) 235
MlyI GAGTC 4 cut(s) 748, 791, 1071, 1229
MmeI TCCRAC 5 cut(s) 57, 168, 681, 986, 1082
Mox20I TGGCCA 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 1032
MscI TGGCCA 1 cut(s) 235
MseI TTAA 5 cut(s) 552, 842, 1155, 1181, 1281
MslI CAYNNNNRTG 2 cut(s) 717, 972
Msp20I TGGCCA 1 cut(s) 235
MspA1I CMGCKG 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 2 cut(s) 83, 1304
MspR9I CCNGG 2 cut(s) 367, 1388
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 2 cut(s) 367, 1388
MvnI CGCG 2 cut(s) 251, 259
NdeII GATC 5 cut(s) 79, 878, 901, 1372, 1376
NlaIV GGNNCC 6 cut(s) 81, 106, 364, 866, 918, 1170
NmuCI GTSAC 2 cut(s) 511, 950
NspI RCATGY 4 cut(s) 357, 404, 971, 1112
PaeI GCATGC 2 cut(s) 404, 1112
PagI TCATGA 1 cut(s) 604
PciI ACATGT 2 cut(s) 353, 967
PctI GAATGC 1 cut(s) 562
PdmI GAANNNNTTC 1 cut(s) 1032
PfeI GAWTC 3 cut(s) 245, 302, 1001
PkrI GCNGC 6 cut(s) 436, 496, 895, 1119, 1140, 1231
Ple19I CGATCG 1 cut(s) 1375
PleI GAGTC 4 cut(s) 747, 790, 1070, 1228
PpsI GAGTC 4 cut(s) 747, 790, 1070, 1228
PscI ACATGT 2 cut(s) 353, 967
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 365, 1386
PspGI CCWGG 2 cut(s) 365, 1386
PspN4I GGNNCC 6 cut(s) 81, 106, 364, 866, 918, 1170
PspPI GGNCC 4 cut(s) 197, 362, 827, 1169
PstI CTGCAG 1 cut(s) 619
PsuI RGATCY 1 cut(s) 79
PvuI CGATCG 1 cut(s) 1375
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 4 cut(s) 53, 573, 866, 1333
RsaNI GTAC 4 cut(s) 52, 572, 865, 1332
RseI CAYNNNNRTG 2 cut(s) 717, 972
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 5 cut(s) 552, 842, 1155, 1181, 1281
SatI GCNGC 6 cut(s) 435, 495, 894, 1118, 1139, 1230
Sau3AI GATC 5 cut(s) 79, 878, 901, 1372, 1376
Sau96I GGNCC 4 cut(s) 197, 362, 827, 1169
SchI GAGTC 4 cut(s) 748, 791, 1071, 1229
ScrFI CCNGG 2 cut(s) 367, 1388
SduI GDGCHC 3 cut(s) 99, 300, 1192
SexAI ACCWGGT 1 cut(s) 1386
SfaNI GCATC 2 cut(s) 473, 879
SfcI CTRYAG 1 cut(s) 615
SinI GGWCC 2 cut(s) 362, 1169
SmiMI CAYNNNNRTG 2 cut(s) 717, 972
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
SphI GCATGC 2 cut(s) 404, 1112
Sse9I AATT 9 cut(s) 152, 201, 288, 328, 595, 671, 745, 1089, 1176
SsiI CCGC 7 cut(s) 136, 210, 249, 259, 360, 560, 868
SspI AATATT 1 cut(s) 789
SspMI CTAG 4 cut(s) 38, 522, 732, 1359
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 2 cut(s) 365, 1386
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 2 cut(s) 62, 1075
TaiI ACGT 1 cut(s) 1083
TaqI TCGA 8 cut(s) 222, 243, 300, 332, 593, 737, 1039, 1375
TasI AATT 9 cut(s) 152, 201, 288, 328, 595, 671, 745, 1089, 1176
TatI WGTACW 3 cut(s) 51, 571, 1331
TfiI GAWTC 3 cut(s) 245, 302, 1001
Tru1I TTAA 5 cut(s) 552, 842, 1155, 1181, 1281
Tru9I TTAA 5 cut(s) 552, 842, 1155, 1181, 1281
TscAI CASTG 3 cut(s) 474, 719, 1452
TseFI GTSAC 2 cut(s) 511, 950
TseI GCWGC 6 cut(s) 434, 494, 893, 1117, 1138, 1229
Tsp45I GTSAC 2 cut(s) 511, 950
TspRI CASTG 3 cut(s) 474, 719, 1452
Vha464I CTTAAG 1 cut(s) 551
VneI GTGCAC 1 cut(s) 1188
VpaK11BI GGWCC 2 cut(s) 362, 1169
XapI RAATTY 3 cut(s) 595, 745, 1176
XceI RCATGY 4 cut(s) 357, 404, 971, 1112
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 1032
XspI CTAG 4 cut(s) 38, 522, 732, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.