Rh5BG312100

HAUS augmin-like complex subunit 6 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002442
Physical Location & Seq
Forward (+)
41202741 .. 41225281
22541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG312100.1

Sequence Viewer

Length: 1005 bp
ATGCTTCATCTTCTTCGGGGACACATTCTGAGATACTGTTCCTTGGAAGAGGTGATCAAGCACTTCATGCATCTATCAACTGAGAAAGCTGAGCAGGCACGTACCCAGGCTCAAGCATTAGAAGAAGCACTTGATGTTGATGACCTCGAAGCAGATAAGAGGCCTAAAGATCTAATGCTGAGTTATGTCAGTGGAGAGAAAGGAAAAGATAGGTCTGATCGTGAAGTTGTTACTCCTTGGTTCAAGTTTTTGTGGGAAACCTACAGAACAGTGCTTGAGATCTTGCGCAACAACTCAAAGTTGGAGGCCCTTTATGCGAATTCTCATTTAGTATCCAAGGCTACTCGTTTGTGGGAGTCGATATTAGCCCGTAAAAGTCAACATGAAGTTCTTGCTTCGGGCCCTATCGAGGATTTAATCGCTCATCGGGAGCATAGGTACCGCATTTCTGGATCTTCTTTGCTTGCAGCTATGGATCAGAGCTCTCAGGTTCCTTATGCAGATGTCTTATCTGTCCAGTCTGGTGATTTTACTCCTACACATGTAGATGATAAAGAGCAGAGTGATGGTTCATATGTCAATATTAACAGAGAAAAGATGAAGAATACTTTAGATTCATCCCATTCACAAGTAAATGATGAAACTCTTCATCGAGCAGATGAGAGAAGTGGAAGAGTCCACCCAACTGTTGACGTGGCAGAAATTATCAGGCGTTGGACACATGCTTTACAGCGTATTCATAAACAGTCACTTCATATGGCAAAAGCTAATGAGGGAGAGGGTCCAGAAATTCTAAGAAGTGCGCATGAAGGCAGTTCAAGTGGTCATGCCGAGTCATTAGCTGCAACACTAGCTGAACATCAGCAGCACTTGGTTAGCTTTCAGGTTCTCATTAATCAACTGAAGGAAGTTGCTCCAGCAATACAAAAGTCAATATCAGAACAGGGAAATTGCGAAACATCTCTCTCTTCCTCCAGTCAAGCTACACTGCAGTATGCTTGCTGA

Protein Analysis

334

Amino Acids

37.64

Weight (kDa)

6.02

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3a_PCI_TPR-like PF22591 13 - 107 2.4e-28 eIF3a, PCI domain, TPR-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 287, 804
Acc65I GGTACC 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 438
AciI CCGC 1 cut(s) 442
AclWI GGATC 2 cut(s) 460, 483
AcsI RAATTY 2 cut(s) 319, 789
AcuI CTGAAG 1 cut(s) 923
AfaI GTAC 2 cut(s) 103, 440
AfiI CCNNNNNNNGG 1 cut(s) 409
AflIII ACRYGT 1 cut(s) 541
AgsI TTSAA 2 cut(s) 244, 819
AjiI CACGTC 1 cut(s) 694
AjnI CCWGG 1 cut(s) 105
AluBI AGCT 8 cut(s) 89, 470, 483, 767, 842, 854, 879, 983
AluI AGCT 8 cut(s) 89, 470, 483, 767, 842, 854, 879, 983
Alw21I GWGCWC 1 cut(s) 485
AlwI GGATC 2 cut(s) 460, 483
AoxI GGCC 3 cut(s) 161, 306, 400
ApaI GGGCCC 1 cut(s) 404
ApeKI GCWGC 3 cut(s) 467, 842, 865
ApoI RAATTY 2 cut(s) 319, 789
AseI ATTAAT 1 cut(s) 894
Asp700I GAANNNNTTC 1 cut(s) 645
Asp718I GGTACC 1 cut(s) 438
AspLEI GCGC 2 cut(s) 288, 805
AspS9I GGNCC 4 cut(s) 307, 400, 401, 782
AsuHPI GGTGA 2 cut(s) 64, 536
AvaII GGWCC 1 cut(s) 782
BaeGI GKGCMC 1 cut(s) 404
BanI GGYRCC 1 cut(s) 438
BanII GRGCYC 2 cut(s) 404, 485
Bbv12I GWGCWC 1 cut(s) 485
BbvI GCAGC 3 cut(s) 479, 829, 877
BccI CCATC 1 cut(s) 560
BciT130I CCWGG 1 cut(s) 107
BciVI GTATCC 1 cut(s) 343
BclI TGATCA 1 cut(s) 54
BfaI CTAG 1 cut(s) 851
BfmI CTRYAG 2 cut(s) 262, 989
BfuI GTATCC 1 cut(s) 343
BglII AGATCT 2 cut(s) 169, 279
BisI GCNGC 3 cut(s) 468, 843, 866
BlpI GCTNAGC 1 cut(s) 90
BlsI GCNGC 3 cut(s) 469, 844, 867
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 1 cut(s) 782
BmgBI CACGTC 1 cut(s) 694
BmgT120I GGNCC 4 cut(s) 307, 400, 401, 782
BmiI GGNNCC 4 cut(s) 402, 440, 492, 783
BmrFI CCNGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 79
BpmI CTGGAG 2 cut(s) 900, 958
Bpu1102I GCTNAGC 1 cut(s) 90
BpuEI CTTGAG 2 cut(s) 96, 296
BsaAI YACGTR 1 cut(s) 101
BsaJI CCNNGG 4 cut(s) 42, 105, 236, 336
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse1I ACTGG 2 cut(s) 517, 975
BseBI CCWGG 1 cut(s) 107
BseDI CCNNGG 4 cut(s) 42, 105, 236, 336
BseGI GGATG 1 cut(s) 617
BseLI CCNNNNNNNGG 1 cut(s) 409
BseMII CTCAG 5 cut(s) 20, 72, 81, 170, 500
BseNI ACTGG 2 cut(s) 517, 975
BseSI GKGCMC 1 cut(s) 404
BseXI GCAGC 3 cut(s) 479, 829, 877
BshFI GGCC 3 cut(s) 163, 308, 402
BshNI GGYRCC 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 485
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 409
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 3 cut(s) 163, 308, 402
Bsp120I GGGCCC 1 cut(s) 400
Bsp1286I GDGCHC 2 cut(s) 404, 485
Bsp143I GATC 6 cut(s) 54, 169, 217, 279, 452, 475
Bsp1720I GCTNAGC 1 cut(s) 90
BspACI CCGC 1 cut(s) 442
BspANI GGCC 3 cut(s) 163, 308, 402
BspCNI CTCAG 5 cut(s) 21, 73, 82, 171, 499
BspLI GGNNCC 4 cut(s) 402, 440, 492, 783
BspMAI CTGCAG 1 cut(s) 993
BspPI GGATC 2 cut(s) 460, 483
BspT107I GGYRCC 1 cut(s) 438
BsrI ACTGG 2 cut(s) 517, 975
BssECI CCNNGG 4 cut(s) 42, 105, 236, 336
BssMI GATC 6 cut(s) 54, 169, 217, 279, 452, 475
BssT1I CCWWGG 3 cut(s) 42, 236, 336
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 4 cut(s) 38, 271, 688, 747
Bst6I CTCTTC 4 cut(s) 42, 651, 667, 973
BstAPI GCANNNNNTGC 1 cut(s) 67
BstBAI YACGTR 1 cut(s) 101
BstC8I GCNNGC 3 cut(s) 96, 465, 1000
BstDEI CTNAG 6 cut(s) 29, 81, 90, 179, 486, 794
BstF5I GGATG 1 cut(s) 617
BstHHI GCGC 2 cut(s) 288, 805
BstKTI GATC 6 cut(s) 57, 172, 220, 282, 455, 478
BstMBI GATC 6 cut(s) 54, 169, 217, 279, 452, 475
BstMWI GCNNNNNNNGC 4 cut(s) 67, 95, 314, 851
BstNI CCWGG 1 cut(s) 107
BstNSI RCATGY 2 cut(s) 545, 725
BstSCI CCNGG 1 cut(s) 105
BstSFI CTRYAG 2 cut(s) 262, 989
BstSLI GKGCMC 1 cut(s) 404
BstV1I GCAGC 3 cut(s) 479, 829, 877
BstX2I RGATCY 3 cut(s) 169, 279, 452
BstYI RGATCY 3 cut(s) 169, 279, 452
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 3 cut(s) 163, 308, 402
BtrI CACGTC 1 cut(s) 694
BtsCI GGATG 1 cut(s) 617
BtsI GCAGTG 1 cut(s) 986
BtsIMutI CAGTG 3 cut(s) 196, 276, 986
Cac8I GCNNGC 3 cut(s) 96, 465, 1000
CfoI GCGC 2 cut(s) 288, 805
Cfr13I GGNCC 4 cut(s) 307, 400, 401, 782
Csp6I GTAC 2 cut(s) 102, 439
CviAII CATG 6 cut(s) 67, 383, 542, 722, 806, 827
CviQI GTAC 2 cut(s) 102, 439
DdeI CTNAG 6 cut(s) 29, 81, 90, 179, 486, 794
DpnI GATC 6 cut(s) 56, 171, 219, 281, 454, 477
DpnII GATC 6 cut(s) 54, 169, 217, 279, 452, 475
Eam1104I CTCTTC 4 cut(s) 42, 651, 667, 973
EarI CTCTTC 4 cut(s) 42, 651, 667, 973
Ecl136II GAGCTC 1 cut(s) 483
Eco130I CCWWGG 3 cut(s) 42, 236, 336
Eco147I AGGCCT 1 cut(s) 163
Eco24I GRGCYC 2 cut(s) 404, 485
Eco47I GGWCC 1 cut(s) 782
Eco53kI GAGCTC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 923
EcoICRI GAGCTC 1 cut(s) 483
EcoO109I RGGNCCY 2 cut(s) 307, 401
EcoRI GAATTC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 105
EcoT14I CCWWGG 3 cut(s) 42, 236, 336
EcoT22I ATGCAT 1 cut(s) 72
EcoT38I GRGCYC 2 cut(s) 404, 485
ErhI CCWWGG 3 cut(s) 42, 236, 336
FaeI CATG 6 cut(s) 70, 386, 545, 725, 809, 830
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FaqI GGGAC 1 cut(s) 33
FatI CATG 6 cut(s) 66, 382, 541, 721, 805, 826
FauNDI CATATG 2 cut(s) 574, 756
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 3 cut(s) 468, 843, 866
FokI GGATG 1 cut(s) 604
FriOI GRGCYC 2 cut(s) 404, 485
Fsp4HI GCNGC 3 cut(s) 468, 843, 866
FspAI RTGCGCAY 1 cut(s) 804
FspBI CTAG 1 cut(s) 851
FspI TGCGCA 2 cut(s) 287, 804
GlaI GCGC 2 cut(s) 287, 804
GluI GCNGC 3 cut(s) 468, 843, 866
GsuI CTGGAG 2 cut(s) 900, 958
HaeIII GGCC 3 cut(s) 163, 308, 402
HhaI GCGC 2 cut(s) 288, 805
Hin1II CATG 6 cut(s) 70, 386, 545, 725, 809, 830
Hin6I GCGC 2 cut(s) 286, 803
HinP1I GCGC 2 cut(s) 286, 803
HincII GTYRAC 2 cut(s) 380, 691
HindII GTYRAC 2 cut(s) 380, 691
HinfI GANTC 4 cut(s) 356, 614, 675, 833
HphI GGTGA 2 cut(s) 64, 536
Hpy166II GTNNAC 3 cut(s) 380, 679, 691
Hpy188I TCNGA 4 cut(s) 30, 217, 480, 940
Hpy188III TCNNGA 4 cut(s) 221, 428, 450, 785
Hpy8I GTNNAC 3 cut(s) 380, 679, 691
HpyAV CCTTC 2 cut(s) 803, 898
HpyCH4III ACNGT 4 cut(s) 38, 271, 688, 747
HpyCH4IV ACGT 2 cut(s) 100, 693
HpyCH4V TGCA 5 cut(s) 70, 467, 500, 845, 991
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 95, 314, 851
HpyF3I CTNAG 6 cut(s) 29, 81, 90, 179, 486, 794
HpySE526I ACGT 2 cut(s) 100, 693
Hsp92II CATG 6 cut(s) 70, 386, 545, 725, 809, 830
HspAI GCGC 2 cut(s) 286, 803
KpnI GGTACC 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 6 cut(s) 54, 169, 217, 279, 452, 475
LmnI GCTCC 2 cut(s) 430, 919
Lsp1109I GCAGC 3 cut(s) 479, 829, 877
LweI GCATC 1 cut(s) 79
MaeI CTAG 1 cut(s) 851
MaeII ACGT 2 cut(s) 100, 693
MaeIII GTNAC 2 cut(s) 229, 747
MalI GATC 6 cut(s) 56, 171, 219, 281, 454, 477
MboI GATC 6 cut(s) 54, 169, 217, 279, 452, 475
MboII GAAGA 8 cut(s) 5, 59, 134, 447, 613, 638, 684, 960
MflI RGATCY 3 cut(s) 169, 279, 452
MhlI GDGCHC 2 cut(s) 404, 485
MluCI AATT 4 cut(s) 319, 702, 789, 949
MlyI GAGTC 3 cut(s) 365, 684, 842
MmeI TCCRAC 2 cut(s) 282, 695
MnlI CCTC 8 cut(s) 43, 153, 155, 298, 403, 766, 772, 982
Mph1103I ATGCAT 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 645
MseI TTAA 3 cut(s) 416, 585, 894
MslI CAYNNNNRTG 1 cut(s) 546
MspR9I CCNGG 1 cut(s) 107
MvaI CCWGG 1 cut(s) 107
MwoI GCNNNNNNNGC 4 cut(s) 67, 95, 314, 851
NdeI CATATG 2 cut(s) 574, 756
NdeII GATC 6 cut(s) 54, 169, 217, 279, 452, 475
NlaIII CATG 6 cut(s) 70, 386, 545, 725, 809, 830
NlaIV GGNNCC 4 cut(s) 402, 440, 492, 783
NmeAIII GCCGAG 1 cut(s) 856
NmuCI GTSAC 1 cut(s) 747
NsbI TGCGCA 2 cut(s) 287, 804
NsiI ATGCAT 1 cut(s) 72
NspI RCATGY 2 cut(s) 545, 725
PceI AGGCCT 1 cut(s) 163
PciI ACATGT 1 cut(s) 541
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 1 cut(s) 614
PkrI GCNGC 3 cut(s) 469, 844, 867
PleI GAGTC 3 cut(s) 364, 683, 841
PpsI GAGTC 3 cut(s) 364, 683, 841
Ppu21I YACGTR 1 cut(s) 101
PscI ACATGT 1 cut(s) 541
PshBI ATTAAT 1 cut(s) 894
Psp124BI GAGCTC 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 4 cut(s) 402, 440, 492, 783
PspOMI GGGCCC 1 cut(s) 400
PspPI GGNCC 4 cut(s) 307, 400, 401, 782
PstI CTGCAG 1 cut(s) 993
PsuI RGATCY 3 cut(s) 169, 279, 452
RsaI GTAC 2 cut(s) 103, 440
RsaNI GTAC 2 cut(s) 102, 439
RseI CAYNNNNRTG 1 cut(s) 546
SacI GAGCTC 1 cut(s) 485
SaqAI TTAA 3 cut(s) 416, 585, 894
SatI GCNGC 3 cut(s) 468, 843, 866
Sau3AI GATC 6 cut(s) 54, 169, 217, 279, 452, 475
Sau96I GGNCC 4 cut(s) 307, 400, 401, 782
SchI GAGTC 3 cut(s) 365, 684, 842
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 2 cut(s) 404, 485
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 2 cut(s) 262, 989
SinI GGWCC 1 cut(s) 782
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 2 cut(s) 111, 275
SmoI CTYRAG 2 cut(s) 111, 275
Sse9I AATT 4 cut(s) 319, 702, 789, 949
SseBI AGGCCT 1 cut(s) 163
SsiI CCGC 1 cut(s) 442
SspI AATATT 1 cut(s) 583
SspMI CTAG 1 cut(s) 851
SstI GAGCTC 1 cut(s) 485
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 1 cut(s) 105
StyI CCWWGG 3 cut(s) 42, 236, 336
TaaI ACNGT 4 cut(s) 38, 271, 688, 747
TaiI ACGT 2 cut(s) 103, 696
TaqI TCGA 4 cut(s) 147, 359, 408, 652
TasI AATT 4 cut(s) 319, 702, 789, 949
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 3 cut(s) 416, 585, 894
Tru9I TTAA 3 cut(s) 416, 585, 894
TscAI CASTG 3 cut(s) 196, 276, 993
TseFI GTSAC 1 cut(s) 747
TseI GCWGC 3 cut(s) 467, 842, 865
Tsp45I GTSAC 1 cut(s) 747
TspRI CASTG 3 cut(s) 196, 276, 993
VpaK11BI GGWCC 1 cut(s) 782
VspI ATTAAT 1 cut(s) 894
XapI RAATTY 2 cut(s) 319, 789
XceI RCATGY 2 cut(s) 545, 725
XmnI GAANNNNTTC 1 cut(s) 645
XspI CTAG 1 cut(s) 851
Zsp2I ATGCAT 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.