Rw7G025810

HAUS augmin-like complex subunit 6 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002442
Physical Location & Seq
Reverse (-)
32547783 .. 32554401
6619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025810.1

Sequence Viewer

Length: 1437 bp
ATGGCCTCCTCCCCCGACCACAACCACACCCTGAACGGCGATTCCAACACCCAGCAATGGCCTCCTCCCCCGGCCATTATCGGACTCGGAGCCTCCAACTCCACTCCCCGGGTCGGGTTCTTCCGCCACTCCAACCCCAAACTCGGCGAGCAGCTCCTCTACTTCATCCTCTCTTCCCTCCGTGGCCCAATTCAATCCGCCAAGGATTTCGATAAGGTCTGGCCAATCTTCGATTCTACGCAATCTCGCGATTTTCGTAAGGTTGTGCAAGGGATTATTAGCGAGCTCGAATCACAAGGGGCGCTTCCCAGGAGCAATTCGAGGGTTTCATCACTTGCTACATGTTGTGGACCGAGGTTGCTGAATATAATTCATTTGATTGTTCTGTTATGGAAATTTTCATTGCTATTTGGAAGAGTTAAGCCCTTTCAGTTCTGCAAGCAATACAAACGAACTACGGAGTTTCAAAGGTTATTCATGATGCTTATCCAAAACCAGTATTTACTAATAATGGCTTGCAAATTGAAATTTTGTGCTTCTTTGGAAATGCTGTCTTTTTTTTTAATTGTTTTTTCAGTATGGTCTTATTTGTTTTTGTTTTTGTGTTATAGTTTGAGTCAGAATTCTCATTTAGTATCCAAGGCTACTCGTTTGTGGGAGTCGATATTATCCCATAAAAGTCAACATGAAGTTCTTGCTTCGGGCCCTATCGAGGATTTAATCGCTCATCGGGAGCATAGCTCTCAGGTTCCTTATGCAGATGTCTTATCTGTCCAGTCTGGTGATTTTACTCCTACACATGTAGATGATAAAGAGCAGAGTGATGGTTCATATGTCAATATTAACAGAGAAAAGATGAAGAATACTTTAGATTCATCCCATTCACAAGTAAATGATGAAACTCTTCATCGAGCAGATGAGAGAAGTGGAAGAGTCCACCCAACTGTTGACGTGGCAGAAATTATCAGGCGTTGGACACATGCTTTACAGCGTATTCATAAACAGTCACTTCATATGGCAAAAGCTAATGAGAGAGAGGGTCCAGAAATTCTAAGAAGTGCGCATGAAGGCAGTTCAAGTGGTCATGCCGAGTCATTAGCTGCAACACTAGCTGAACATCAGCAGCACTTGGTTAGCTTTCAGGTTCTCATTAATCAACTGAAGGAAGTTGCTCCAGCAATACAAAAGTCAATATCAGAACAGGTTGACTTCTTTACTTCTGCTTGTATTTCAAGCTACAATGCAGTATGCTTGCTGAGGATGCCATCAAGGCAGCTGTTAAAGACTATCAAACAAAGCTGGCTAATGAAGGATTGCTTATCATCTTTGCCTACAAGAGAGATTCTGCAAGAGCAAATGAAGGTAATTCACACAACGGAAACTAGTATTCTGTTGAGCAAAGTGATATCACACAACGGTGAAATCACCGTTGTCTGA

Protein Analysis

478

Amino Acids

54.1

Weight (kDa)

8.64

Isoelectric Point (pI)

51.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAUS6_N PF14661 37 - 124 7.7e-18 HAUS augmin-like complex subunit 6 N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1062
AccII CGCG 1 cut(s) 249
AciI CCGC 2 cut(s) 124, 198
AcoI YGGCCR 2 cut(s) 72, 221
AcsI RAATTY 4 cut(s) 395, 527, 622, 1047
AcuI CTGAAG 1 cut(s) 1181
AfiI CCNNNNNNNGG 6 cut(s) 57, 108, 113, 114, 143, 712
AflIII ACRYGT 2 cut(s) 341, 799
AgsI TTSAA 5 cut(s) 194, 467, 526, 1077, 1233
AhlI ACTAGT 1 cut(s) 1382
AjiI CACGTC 1 cut(s) 952
AjnI CCWGG 1 cut(s) 308
AjuI GAANNNNNNNTTGG 2 cut(s) 217, 249
AleI CACNNNNGTG 1 cut(s) 1416
AloI GAACNNNNNNTCC 2 cut(s) 26, 58
Alw21I GWGCWC 1 cut(s) 288
Ama87I CYCGRG 1 cut(s) 108
AoxI GGCC 6 cut(s) 3, 59, 72, 184, 221, 703
ApaI GGGCCC 1 cut(s) 707
ApeKI GCWGC 4 cut(s) 151, 1100, 1123, 1273
ApoI RAATTY 4 cut(s) 395, 527, 622, 1047
AseI ATTAAT 1 cut(s) 1152
Asp700I GAANNNNTTC 1 cut(s) 903
AspLEI GCGC 2 cut(s) 304, 1063
AspS9I GGNCC 5 cut(s) 185, 350, 703, 704, 1040
AsuC2I CCSGG 3 cut(s) 71, 109, 110
AsuHPI GGTGA 3 cut(s) 794, 1417, 1430
AvaI CYCGRG 1 cut(s) 108
AvaII GGWCC 2 cut(s) 350, 1040
BaeGI GKGCMC 1 cut(s) 707
BalI TGGCCA 1 cut(s) 223
BanII GRGCYC 2 cut(s) 288, 707
Bbv12I GWGCWC 1 cut(s) 288
BbvCI CCTCAGC 1 cut(s) 1256
BbvI GCAGC 4 cut(s) 163, 1087, 1135, 1285
BccI CCATC 2 cut(s) 818, 1273
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 310
BciVI GTATCC 1 cut(s) 646
BcnI CCSGG 3 cut(s) 71, 109, 110
BcuI ACTAGT 1 cut(s) 1382
BfaI CTAG 2 cut(s) 1109, 1383
BfoI RGCGCY 1 cut(s) 305
BfuI GTATCC 1 cut(s) 646
BglI GCCNNNNNGGC 1 cut(s) 1270
BisI GCNGC 4 cut(s) 152, 1101, 1124, 1274
BlsI GCNGC 4 cut(s) 153, 1102, 1125, 1275
Bme1390I CCNGG 4 cut(s) 71, 109, 110, 310
Bme18I GGWCC 2 cut(s) 350, 1040
BmeT110I CYCGRG 1 cut(s) 108
BmgBI CACGTC 1 cut(s) 952
BmgT120I GGNCC 5 cut(s) 185, 350, 703, 704, 1040
BmiI GGNNCC 4 cut(s) 91, 705, 750, 1041
BmrFI CCNGG 4 cut(s) 71, 109, 110, 310
BmsI GCATC 2 cut(s) 471, 1251
BplI GAGNNNNNCTC 2 cut(s) 725, 757
BpmI CTGGAG 1 cut(s) 1158
Bpu10I CCTNAGC 1 cut(s) 1256
BpuMI CCSGG 3 cut(s) 71, 109, 110
BsaBI GATNNNNATC 1 cut(s) 485
BsaJI CCNNGG 8 cut(s) 69, 107, 108, 181, 201, 308, 353, 639
Bsc4I CCNNNNNNNGG 6 cut(s) 57, 108, 113, 114, 143, 712
Bse1I ACTGG 2 cut(s) 496, 775
Bse3DI GCAATG 2 cut(s) 62, 401
Bse8I GATNNNNATC 1 cut(s) 485
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 8 cut(s) 69, 107, 108, 181, 201, 308, 353, 639
BseGI GGATG 3 cut(s) 165, 875, 1266
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 6 cut(s) 57, 108, 113, 114, 143, 712
BseMI GCAATG 2 cut(s) 62, 401
BseMII CTCAG 2 cut(s) 758, 1247
BseNI ACTGG 2 cut(s) 496, 775
BseRI GAGGAG 2 cut(s) 54, 146
BseSI GKGCMC 1 cut(s) 707
BseXI GCAGC 4 cut(s) 163, 1087, 1135, 1285
BseYI CCCAGC 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 6 cut(s) 5, 61, 74, 186, 223, 705
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 108
BsiSI CCGG 2 cut(s) 71, 109
BslI CCNNNNNNNGG 6 cut(s) 57, 108, 113, 114, 143, 712
BsnI GGCC 6 cut(s) 5, 61, 74, 186, 223, 705
BsoBI CYCGRG 1 cut(s) 108
Bsp120I GGGCCC 1 cut(s) 703
Bsp1286I GDGCHC 2 cut(s) 288, 707
Bsp68I TCGCGA 1 cut(s) 249
BspACI CCGC 2 cut(s) 124, 198
BspANI GGCC 6 cut(s) 5, 61, 74, 186, 223, 705
BspCNI CTCAG 2 cut(s) 757, 1248
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 4 cut(s) 91, 705, 750, 1041
BsrDI GCAATG 2 cut(s) 62, 401
BsrI ACTGG 2 cut(s) 496, 775
BssECI CCNNGG 8 cut(s) 69, 107, 108, 181, 201, 308, 353, 639
BssT1I CCWWGG 2 cut(s) 201, 639
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 4 cut(s) 946, 1005, 1418, 1429
Bst6I CTCTTC 4 cut(s) 178, 409, 909, 925
BstC8I GCNNGC 6 cut(s) 149, 284, 440, 517, 1253, 1301
BstDEI CTNAG 3 cut(s) 744, 1052, 1256
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 3 cut(s) 165, 875, 1266
BstFNI CGCG 1 cut(s) 249
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 2 cut(s) 304, 1063
BstMWI GCNNNNNNNGC 3 cut(s) 1109, 1261, 1270
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 3 cut(s) 345, 803, 983
BstSCI CCNGG 4 cut(s) 69, 107, 108, 308
BstSLI GKGCMC 1 cut(s) 707
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 4 cut(s) 163, 1087, 1135, 1285
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 6 cut(s) 5, 61, 74, 186, 223, 705
BtgI CCRYGG 1 cut(s) 181
BtrI CACGTC 1 cut(s) 952
BtsCI GGATG 3 cut(s) 165, 875, 1266
BtuMI TCGCGA 1 cut(s) 249
Cac8I GCNNGC 6 cut(s) 149, 284, 440, 517, 1253, 1301
CciI TCATGA 1 cut(s) 477
CfoI GCGC 2 cut(s) 304, 1063
Cfr13I GGNCC 5 cut(s) 185, 350, 703, 704, 1040
Cfr9I CCCGGG 1 cut(s) 108
CviAII CATG 7 cut(s) 342, 478, 686, 800, 980, 1064, 1085
DdeI CTNAG 3 cut(s) 744, 1052, 1256
EaeI YGGCCR 2 cut(s) 72, 221
Eam1104I CTCTTC 4 cut(s) 178, 409, 909, 925
EarI CTCTTC 4 cut(s) 178, 409, 909, 925
EciI GGCGGA 2 cut(s) 113, 187
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 201, 639
Eco24I GRGCYC 2 cut(s) 288, 707
Eco32I GATATC 1 cut(s) 1407
Eco47I GGWCC 2 cut(s) 350, 1040
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 1181
Eco88I CYCGRG 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 704
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 308
EcoRV GATATC 1 cut(s) 1407
EcoT14I CCWWGG 2 cut(s) 201, 639
EcoT38I GRGCYC 2 cut(s) 288, 707
ErhI CCWWGG 2 cut(s) 201, 639
FaeI CATG 7 cut(s) 345, 481, 689, 803, 983, 1067, 1088
FalI AAGNNNNNCTT 4 cut(s) 288, 320, 1301, 1333
FatI CATG 7 cut(s) 341, 477, 685, 799, 979, 1063, 1084
FauNDI CATATG 2 cut(s) 832, 1014
Fnu4HI GCNGC 4 cut(s) 152, 1101, 1124, 1274
FokI GGATG 3 cut(s) 152, 862, 1273
FriOI GRGCYC 2 cut(s) 288, 707
Fsp4HI GCNGC 4 cut(s) 152, 1101, 1124, 1274
FspAI RTGCGCAY 1 cut(s) 1062
FspBI CTAG 2 cut(s) 1109, 1383
FspI TGCGCA 1 cut(s) 1062
GlaI GCGC 2 cut(s) 303, 1062
GluI GCNGC 4 cut(s) 152, 1101, 1124, 1274
GsaI CCCAGC 1 cut(s) 55
GsuI CTGGAG 1 cut(s) 1158
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 6 cut(s) 5, 61, 74, 186, 223, 705
HapII CCGG 2 cut(s) 71, 109
HhaI GCGC 2 cut(s) 304, 1063
Hin1II CATG 7 cut(s) 345, 481, 689, 803, 983, 1067, 1088
Hin6I GCGC 2 cut(s) 302, 1061
HinP1I GCGC 2 cut(s) 302, 1061
HincII GTYRAC 3 cut(s) 683, 949, 1207
HindII GTYRAC 3 cut(s) 683, 949, 1207
HpaII CCGG 2 cut(s) 71, 109
HphI GGTGA 3 cut(s) 794, 1417, 1430
Hpy166II GTNNAC 5 cut(s) 350, 683, 937, 949, 1207
Hpy188I TCNGA 5 cut(s) 83, 89, 621, 1198, 1436
Hpy188III TCNNGA 4 cut(s) 248, 478, 731, 1043
Hpy8I GTNNAC 5 cut(s) 350, 683, 937, 949, 1207
HpyAV CCTTC 4 cut(s) 1061, 1156, 1303, 1354
HpyCH4III ACNGT 4 cut(s) 946, 1005, 1418, 1429
HpyCH4IV ACGT 1 cut(s) 951
HpyCH4V TGCA 7 cut(s) 268, 438, 519, 758, 1103, 1244, 1348
HpyF10VI GCNNNNNNNGC 3 cut(s) 1109, 1261, 1270
HpyF3I CTNAG 3 cut(s) 744, 1052, 1256
HpySE526I ACGT 1 cut(s) 951
Hsp92II CATG 7 cut(s) 345, 481, 689, 803, 983, 1067, 1088
HspAI GCGC 2 cut(s) 302, 1061
LmnI GCTCC 5 cut(s) 89, 159, 312, 733, 1177
Lsp1109I GCAGC 4 cut(s) 163, 1087, 1135, 1285
LweI GCATC 2 cut(s) 471, 1251
MaeI CTAG 2 cut(s) 1109, 1383
MaeII ACGT 1 cut(s) 951
MaeIII GTNAC 1 cut(s) 1005
MboII GAAGA 7 cut(s) 112, 165, 220, 426, 871, 896, 942
MhlI GDGCHC 2 cut(s) 288, 707
MlsI TGGCCA 1 cut(s) 223
MluNI TGGCCA 1 cut(s) 223
MlyI GAGTC 5 cut(s) 78, 625, 668, 942, 1100
MmeI TCCRAC 4 cut(s) 69, 120, 156, 953
Mox20I TGGCCA 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 903
MscI TGGCCA 1 cut(s) 223
MseI TTAA 6 cut(s) 420, 563, 719, 843, 1152, 1280
MslI CAYNNNNRTG 2 cut(s) 804, 1416
Msp20I TGGCCA 1 cut(s) 223
MspA1I CMGCKG 1 cut(s) 1276
MspI CCGG 2 cut(s) 71, 109
MspR9I CCNGG 4 cut(s) 71, 109, 110, 310
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 3 cut(s) 1109, 1261, 1270
NciI CCSGG 3 cut(s) 71, 109, 110
NdeI CATATG 2 cut(s) 832, 1014
NlaIII CATG 7 cut(s) 345, 481, 689, 803, 983, 1067, 1088
NlaIV GGNNCC 4 cut(s) 91, 705, 750, 1041
NmeAIII GCCGAG 2 cut(s) 123, 1114
NmuCI GTSAC 1 cut(s) 1005
NruI TCGCGA 1 cut(s) 249
NsbI TGCGCA 1 cut(s) 1062
NspI RCATGY 3 cut(s) 345, 803, 983
OliI CACNNNNGTG 1 cut(s) 1416
PagI TCATGA 1 cut(s) 477
PciI ACATGT 2 cut(s) 341, 799
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 5 cut(s) 41, 233, 290, 872, 1342
PkrI GCNGC 4 cut(s) 153, 1102, 1125, 1275
PleI GAGTC 5 cut(s) 78, 624, 667, 941, 1099
PpsI GAGTC 5 cut(s) 78, 624, 667, 941, 1099
PscI ACATGT 2 cut(s) 341, 799
PshBI ATTAAT 1 cut(s) 1152
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 308
PspFI CCCAGC 1 cut(s) 51
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 4 cut(s) 91, 705, 750, 1041
PspOMI GGGCCC 1 cut(s) 703
PspPI GGNCC 5 cut(s) 185, 350, 703, 704, 1040
PvuII CAGCTG 1 cut(s) 1276
RruI TCGCGA 1 cut(s) 249
RseI CAYNNNNRTG 2 cut(s) 804, 1416
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 6 cut(s) 420, 563, 719, 843, 1152, 1280
SatI GCNGC 4 cut(s) 152, 1101, 1124, 1274
Sau96I GGNCC 5 cut(s) 185, 350, 703, 704, 1040
SchI GAGTC 5 cut(s) 78, 625, 668, 942, 1100
ScrFI CCNGG 4 cut(s) 71, 109, 110, 310
SduI GDGCHC 2 cut(s) 288, 707
SfaNI GCATC 2 cut(s) 471, 1251
SinI GGWCC 2 cut(s) 350, 1040
SmaI CCCGGG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 804, 1416
SpeI ACTAGT 1 cut(s) 1382
SsiI CCGC 2 cut(s) 124, 198
SspI AATATT 1 cut(s) 841
SspMI CTAG 2 cut(s) 1109, 1383
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 4 cut(s) 69, 107, 108, 308
StyI CCWWGG 2 cut(s) 201, 639
TaaI ACNGT 4 cut(s) 946, 1005, 1418, 1429
TaiI ACGT 1 cut(s) 954
TaqI TCGA 7 cut(s) 210, 231, 288, 320, 662, 711, 910
TaqII GACCGA 1 cut(s) 367
TfiI GAWTC 5 cut(s) 41, 233, 290, 872, 1342
Tru1I TTAA 6 cut(s) 420, 563, 719, 843, 1152, 1280
Tru9I TTAA 6 cut(s) 420, 563, 719, 843, 1152, 1280
TseFI GTSAC 1 cut(s) 1005
TseI GCWGC 4 cut(s) 151, 1100, 1123, 1273
Tsp45I GTSAC 1 cut(s) 1005
TspGWI ACGGA 3 cut(s) 170, 473, 1391
TspMI CCCGGG 1 cut(s) 108
VpaK11BI GGWCC 2 cut(s) 350, 1040
VspI ATTAAT 1 cut(s) 1152
XapI RAATTY 4 cut(s) 395, 527, 622, 1047
XceI RCATGY 3 cut(s) 345, 803, 983
XmaI CCCGGG 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 2 cut(s) 1109, 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.