pycom17g01560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004339
Physical Location & Seq
Forward (+)
1037862 .. 1039783
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1515 bp
ATGTTGAACTTAGAGTGCATTAACACTTTAAATCAGGGTCTTTTACTACAACATGCAGCAAATGGTTGCCCAAAGCCAAAGTGGACAAAATACACGAGCTTTCTGAAAAGCAAAACTTTTGCTGAACTGACTCGGCCAAAACAGCTGACTGCTGCTACTTTACGATTTGAGGAGCCAGTACATGTAGTACAGGAAGTGCAGCAGGAGGTTGTCGATGAACCTGGAAAACCATATCCAAAAATCCACACCCTTTTTTCTACAGAATGCACCCCTTACTTTGATTGGCAGACTGTAGGACTTGTCCACAGTTTTCACCTGAGTGGCCAGCCTGGAAATATCACACGATTACTCAGCTGTACGGATGAAGACTTGAAGCAATATGCTGGGCATGATCTGGCTCCCACCCATTATGTTCCTTCCATGAGCCGACATCCATTAACGGGTGATTGGTATCCAGCAATTAACAAACCAGCTGCAGTCCTTCATTGGCTCAATCATGCAAATACGGATGCAGAATACATAGTGATTCTTGATGCTGACATGATCTTGAGGGGTCCAATTACCCCATGGGAATTCAAAGCAGCACAGGGACGACCAGTTTCGACTCCCTATGGCTATCTTATCGGCTGTGACAATGAGCTTGCAAAACTCCATACTCGACATCCTGAAGCCTGTGACAAAGTTGGGGGTGTTATCATCATGCACATAGATGATCTTAGAAAGTTTGCTCTACTGTGGTTGCACAAAACCGAGGAAGTCCGGGCAGACACAGCTCATTATGCTACAAATATCACTGGAGATATTTATGCATCTGGTTGGATCAGTGAGATGTATGGTTACTCATTTGGGGCAGCAGAGTTGAAACTACGGCATCAAATAAGCAGCGAGATACTGATATACCCGGGGTATGCTCCTGCACCAGGCATCAAATACAGAGTATTCCACTACGGTTTGGAATTCAAGGTTGGAAACTGGAGCTTTGATAAAGCAAACTGGAGGAATGTTGATGTGGTTAACAAGTGCTGGGCCCAGTTTCCAGACCCACCCGGCCCTTCAACACTCGACCGGACAGACAATAACAAACTGCAGACAGATCTGCTTAGCCTAGAATGCATTAAGACACTAAATGAAGCGTTACACCTGCATCACAAGAGAAGGAACTGTCCAGATCCAAACTCAGATGCAGCAGAGGAAGTTATAGTATCAAGGAAACTCGGTAAGTTGGATGGAAGTTTTGGTCTAGGAAGCAACCGTGTCCAAACGAATCATTCCGAGGAGATTCCAGAACCTACATTGACTGATGGGATGTTTAGTTCTGTGAGGTTCTTGGTTATTGCTCTATGGGCATTCTGTGGCTTGGGCTTCTTAACGATCGCGTGGGTGCTGTTTTCAGGTCGTAAAGGGAAAGGTAAAAGAGGTAAAAGTTATAAAATCAAGAGAAGAAATTCTGGTACAGGATTTATGGATATTAGCAGCCGTGATAGGCATTTACGTGGAGTTGAAGTACCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
No domains found.

Protein Analysis

505

Amino Acids

56.78

Weight (kDa)

7.98

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HPAT PF23452 82 - 335 1.5e-18 Hydroxyproline O-arabinosyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1428
AarI CACCTGC 1 cut(s) 1149
Acc36I ACCTGC 1 cut(s) 1149
AccII CGCG 1 cut(s) 1376
AclWI GGATC 2 cut(s) 827, 1163
AcoI YGGCCR 2 cut(s) 134, 322
AcsI RAATTY 3 cut(s) 572, 956, 1444
AcuI CTGAAG 1 cut(s) 687
AfaI GTAC 5 cut(s) 180, 189, 358, 1453, 1506
AfiI CCNNNNNNNGG 2 cut(s) 440, 920
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 7 cut(s) 7, 373, 577, 862, 961, 1056, 1502
AjnI CCWGG 3 cut(s) 220, 328, 919
AjuI GAANNNNNNNTTGG 2 cut(s) 948, 980
AleI CACNNNNGTG 1 cut(s) 318
AloI GAACNNNNNNTCC 2 cut(s) 1297, 1329
AluBI AGCT 7 cut(s) 99, 145, 354, 473, 640, 773, 978
AluI AGCT 7 cut(s) 99, 145, 354, 473, 640, 773, 978
AlwI GGATC 2 cut(s) 827, 1163
Ama87I CYCGRG 1 cut(s) 901
AoxI GGCC 4 cut(s) 134, 322, 1026, 1048
ApaI GGGCCC 1 cut(s) 1030
ApeKI GCWGC 9 cut(s) 56, 152, 199, 473, 581, 851, 882, 1184, 1473
ApoI RAATTY 3 cut(s) 572, 956, 1444
Asp700I GAANNNNTTC 1 cut(s) 1444
AspS9I GGNCC 4 cut(s) 554, 1026, 1027, 1049
AsuC2I CCSGG 4 cut(s) 761, 902, 903, 1047
AsuHPI GGTGA 2 cut(s) 305, 455
AvaI CYCGRG 1 cut(s) 901
AvaII GGWCC 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 1030
BalI TGGCCA 1 cut(s) 324
BanII GRGCYC 1 cut(s) 1030
BauI CACGAG 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 372
BbvI GCAGC 9 cut(s) 68, 139, 211, 460, 593, 863, 894, 1196, 1485
BccI CCATC 2 cut(s) 1220, 1295
BceAI ACGGC 2 cut(s) 884, 1461
BciT130I CCWGG 3 cut(s) 222, 330, 921
BciVI GTATCC 1 cut(s) 462
BcnI CCSGG 4 cut(s) 761, 902, 903, 1047
BfaI CTAG 2 cut(s) 1106, 1241
BfmI CTRYAG 4 cut(s) 258, 291, 474, 1085
BfuAI ACCTGC 1 cut(s) 1149
BfuI GTATCC 1 cut(s) 462
BglII AGATCT 1 cut(s) 1093
BisI GCNGC 9 cut(s) 57, 153, 200, 474, 582, 852, 883, 1185, 1474
BlpI GCTNAGC 1 cut(s) 1100
BlsI GCNGC 9 cut(s) 58, 154, 201, 475, 583, 853, 884, 1186, 1475
Bme1390I CCNGG 7 cut(s) 222, 330, 761, 902, 903, 921, 1047
Bme18I GGWCC 1 cut(s) 554
BmeT110I CYCGRG 1 cut(s) 901
BmgT120I GGNCC 4 cut(s) 554, 1026, 1027, 1049
BmiI GGNNCC 4 cut(s) 174, 399, 555, 1028
BmrFI CCNGG 7 cut(s) 222, 330, 761, 902, 903, 921, 1047
BmrI ACTGGG 1 cut(s) 1024
BmsI GCATC 7 cut(s) 499, 523, 818, 880, 933, 1153, 1171
BmuI ACTGGG 1 cut(s) 1024
BpiI GAAGAC 1 cut(s) 372
BpmI CTGGAG 3 cut(s) 816, 994, 1015
Bpu1102I GCTNAGC 1 cut(s) 1100
BpuEI CTTGAG 1 cut(s) 568
BpuMI CCSGG 4 cut(s) 761, 902, 903, 1047
BsaAI YACGTR 1 cut(s) 1493
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 5 cut(s) 566, 750, 901, 902, 1272
BsaWI WCCGGW 1 cut(s) 1065
BsaXI ACNNNNNCTCC 1 cut(s) 1488
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 920
Bse1I ACTGG 6 cut(s) 176, 596, 799, 977, 998, 1030
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 3 cut(s) 222, 330, 921
BseDI CCNNGG 5 cut(s) 566, 750, 901, 902, 1272
BseGI GGATG 6 cut(s) 367, 430, 514, 661, 1231, 1311
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 2 cut(s) 440, 920
BseMII CTCAG 3 cut(s) 308, 364, 1191
BseNI ACTGG 6 cut(s) 176, 596, 799, 977, 998, 1030
BseRI GAGGAG 2 cut(s) 185, 1289
BseSI GKGCMC 1 cut(s) 1030
BseXI GCAGC 9 cut(s) 68, 139, 211, 460, 593, 863, 894, 1196, 1485
BseYI CCCAGC 2 cut(s) 383, 1023
BsgI GTGCAG 2 cut(s) 218, 900
Bsh1236I CGCG 1 cut(s) 1376
Bsh1285I CGRYCG 2 cut(s) 1066, 1374
BshFI GGCC 4 cut(s) 136, 324, 1028, 1050
BsiEI CGRYCG 2 cut(s) 1066, 1374
BsiHKCI CYCGRG 1 cut(s) 901
BsiSI CCGG 4 cut(s) 760, 902, 1047, 1066
BslFI GGGAC 1 cut(s) 603
BslI CCNNNNNNNGG 2 cut(s) 440, 920
BsmFI GGGAC 1 cut(s) 603
BsmI GAATGC 3 cut(s) 269, 1115, 1346
BsnI GGCC 4 cut(s) 136, 324, 1028, 1050
BsoBI CYCGRG 1 cut(s) 901
Bsp120I GGGCCC 1 cut(s) 1026
Bsp1286I GDGCHC 1 cut(s) 1030
Bsp143I GATC 7 cut(s) 391, 543, 712, 819, 1093, 1168, 1371
Bsp1720I GCTNAGC 1 cut(s) 1100
Bsp19I CCATGG 1 cut(s) 566
BspANI GGCC 4 cut(s) 136, 324, 1028, 1050
BspCNI CTCAG 3 cut(s) 309, 363, 1190
BspFNI CGCG 1 cut(s) 1376
BspLI GGNNCC 4 cut(s) 174, 399, 555, 1028
BspMAI CTGCAG 2 cut(s) 478, 1089
BspMI ACCTGC 1 cut(s) 1149
BspPI GGATC 2 cut(s) 827, 1163
BsrI ACTGG 6 cut(s) 176, 596, 799, 977, 998, 1030
BssECI CCNNGG 5 cut(s) 566, 750, 901, 902, 1272
BssMI GATC 7 cut(s) 391, 543, 712, 819, 1093, 1168, 1371
BssSI CACGAG 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 566
Bst2BI CACGAG 1 cut(s) 94
Bst2UI CCWGG 3 cut(s) 222, 330, 921
Bst4CI ACNGT 6 cut(s) 292, 308, 735, 950, 1163, 1253
BstBAI YACGTR 1 cut(s) 1493
BstC8I GCNNGC 2 cut(s) 326, 642
BstDEI CTNAG 6 cut(s) 10, 317, 350, 716, 1100, 1177
BstDSI CCRYGG 1 cut(s) 566
BstENI CCTNNNNNAGG 1 cut(s) 918
BstF5I GGATG 6 cut(s) 367, 430, 514, 661, 1231, 1311
BstFNI CGCG 1 cut(s) 1376
BstKTI GATC 7 cut(s) 394, 546, 715, 822, 1096, 1171, 1374
BstMBI GATC 7 cut(s) 391, 543, 712, 819, 1093, 1168, 1371
BstMCI CGRYCG 2 cut(s) 1066, 1374
BstMWI GCNNNNNNNGC 5 cut(s) 142, 770, 779, 1110, 1343
BstNI CCWGG 3 cut(s) 222, 330, 921
BstNSI RCATGY 2 cut(s) 56, 185
BstSCI CCNGG 7 cut(s) 220, 328, 759, 900, 901, 919, 1045
BstSFI CTRYAG 4 cut(s) 258, 291, 474, 1085
BstSLI GKGCMC 1 cut(s) 1030
BstUI CGCG 1 cut(s) 1376
BstV1I GCAGC 9 cut(s) 68, 139, 211, 460, 593, 863, 894, 1196, 1485
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 2 cut(s) 1093, 1168
BstYI RGATCY 2 cut(s) 1093, 1168
BsuI GTATCC 1 cut(s) 462
BsuRI GGCC 4 cut(s) 136, 324, 1028, 1050
BtgI CCRYGG 1 cut(s) 566
BtsCI GGATG 6 cut(s) 367, 430, 514, 661, 1231, 1311
BtsIMutI CAGTG 2 cut(s) 792, 829
BveI ACCTGC 1 cut(s) 1149
Cac8I GCNNGC 2 cut(s) 326, 642
Cfr13I GGNCC 4 cut(s) 554, 1026, 1027, 1049
Cfr9I CCCGGG 1 cut(s) 901
Csp6I GTAC 5 cut(s) 179, 188, 357, 1452, 1505
CviAII CATG 8 cut(s) 53, 182, 389, 421, 497, 541, 567, 700
CviQI GTAC 5 cut(s) 179, 188, 357, 1452, 1505
DdeI CTNAG 6 cut(s) 10, 317, 350, 716, 1100, 1177
DpnI GATC 7 cut(s) 393, 545, 714, 821, 1095, 1170, 1373
DpnII GATC 7 cut(s) 391, 543, 712, 819, 1093, 1168, 1371
DraI TTTAAA 1 cut(s) 30
EaeI YGGCCR 2 cut(s) 134, 322
Eco130I CCWWGG 1 cut(s) 566
Eco24I GRGCYC 1 cut(s) 1030
Eco47I GGWCC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 687
Eco88I CYCGRG 1 cut(s) 901
EcoNI CCTNNNNNAGG 1 cut(s) 918
EcoRI GAATTC 2 cut(s) 572, 956
EcoRII CCWGG 3 cut(s) 220, 328, 919
EcoT14I CCWWGG 1 cut(s) 566
EcoT22I ATGCAT 2 cut(s) 811, 1115
EcoT38I GRGCYC 1 cut(s) 1030
ErhI CCWWGG 1 cut(s) 566
FaeI CATG 8 cut(s) 56, 185, 392, 424, 500, 544, 570, 703
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FaqI GGGAC 1 cut(s) 603
FatI CATG 8 cut(s) 52, 181, 388, 420, 496, 540, 566, 699
Fnu4HI GCNGC 9 cut(s) 57, 153, 200, 474, 582, 852, 883, 1185, 1474
FokI GGATG 6 cut(s) 374, 417, 521, 648, 1238, 1318
FriOI GRGCYC 1 cut(s) 1030
Fsp4HI GCNGC 9 cut(s) 57, 153, 200, 474, 582, 852, 883, 1185, 1474
FspBI CTAG 2 cut(s) 1106, 1241
GluI GCNGC 9 cut(s) 57, 153, 200, 474, 582, 852, 883, 1185, 1474
GsaI CCCAGC 2 cut(s) 387, 1027
GsuI CTGGAG 3 cut(s) 816, 994, 1015
HaeIII GGCC 4 cut(s) 136, 324, 1028, 1050
HapII CCGG 4 cut(s) 760, 902, 1047, 1066
Hin1II CATG 8 cut(s) 56, 185, 392, 424, 500, 544, 570, 703
HincII GTYRAC 1 cut(s) 1015
HindII GTYRAC 1 cut(s) 1015
HinfI GANTC 5 cut(s) 130, 526, 604, 1264, 1279
HpaI GTTAAC 1 cut(s) 1015
HpaII CCGG 4 cut(s) 760, 902, 1047, 1066
HphI GGTGA 2 cut(s) 305, 455
Hpy166II GTNNAC 3 cut(s) 84, 304, 1015
Hpy188I TCNGA 3 cut(s) 105, 1180, 1273
Hpy188III TCNNGA 7 cut(s) 530, 547, 665, 1037, 1166, 1283, 1435
Hpy8I GTNNAC 3 cut(s) 84, 304, 1015
HpyAV CCTTC 4 cut(s) 426, 491, 1062, 1149
HpyCH4III ACNGT 6 cut(s) 292, 308, 735, 950, 1163, 1253
HpyCH4IV ACGT 1 cut(s) 1492
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 770, 779, 1110, 1343
HpyF3I CTNAG 6 cut(s) 10, 317, 350, 716, 1100, 1177
HpySE526I ACGT 1 cut(s) 1492
Hsp92II CATG 8 cut(s) 56, 185, 392, 424, 500, 544, 570, 703
KspAI GTTAAC 1 cut(s) 1015
Kzo9I GATC 7 cut(s) 391, 543, 712, 819, 1093, 1168, 1371
LmnI GCTCC 4 cut(s) 172, 403, 916, 975
Lsp1109I GCAGC 9 cut(s) 68, 139, 211, 460, 593, 863, 894, 1196, 1485
LweI GCATC 7 cut(s) 499, 523, 818, 880, 933, 1153, 1171
MaeI CTAG 2 cut(s) 1106, 1241
MaeII ACGT 1 cut(s) 1492
MaeIII GTNAC 4 cut(s) 629, 674, 836, 1134
MalI GATC 7 cut(s) 393, 545, 714, 821, 1095, 1170, 1373
MboI GATC 7 cut(s) 391, 543, 712, 819, 1093, 1168, 1371
MboII GAAGA 2 cut(s) 377, 1452
MflI RGATCY 2 cut(s) 1093, 1168
MhlI GDGCHC 1 cut(s) 1030
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 5 cut(s) 459, 558, 572, 956, 1444
MluNI TGGCCA 1 cut(s) 324
MlyI GAGTC 2 cut(s) 124, 598
MmeI TCCRAC 3 cut(s) 797, 946, 1203
MnlI CCTC 9 cut(s) 163, 199, 543, 745, 990, 1183, 1267, 1314, 1409
Mox20I TGGCCA 1 cut(s) 324
Mph1103I ATGCAT 2 cut(s) 811, 1115
MroXI GAANNNNTTC 1 cut(s) 1444
MscI TGGCCA 1 cut(s) 324
MseI TTAA 7 cut(s) 21, 29, 437, 462, 1014, 1116, 1367
MslI CAYNNNNRTG 3 cut(s) 318, 708, 1491
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 3 cut(s) 145, 354, 473
MspI CCGG 4 cut(s) 760, 902, 1047, 1066
MspR9I CCNGG 7 cut(s) 222, 330, 761, 902, 903, 921, 1047
Mva1269I GAATGC 3 cut(s) 269, 1115, 1346
MvaI CCWGG 3 cut(s) 222, 330, 921
MvnI CGCG 1 cut(s) 1376
MwoI GCNNNNNNNGC 5 cut(s) 142, 770, 779, 1110, 1343
NciI CCSGG 4 cut(s) 761, 902, 903, 1047
NcoI CCATGG 1 cut(s) 566
NdeII GATC 7 cut(s) 391, 543, 712, 819, 1093, 1168, 1371
NlaIII CATG 8 cut(s) 56, 185, 392, 424, 500, 544, 570, 703
NlaIV GGNNCC 4 cut(s) 174, 399, 555, 1028
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 629, 674
NsiI ATGCAT 2 cut(s) 811, 1115
NspI RCATGY 2 cut(s) 56, 185
OliI CACNNNNGTG 1 cut(s) 318
PaqCI CACCTGC 1 cut(s) 1149
PciI ACATGT 1 cut(s) 181
PctI GAATGC 3 cut(s) 269, 1115, 1346
PdmI GAANNNNTTC 1 cut(s) 1444
PfeI GAWTC 3 cut(s) 526, 1264, 1279
PkrI GCNGC 9 cut(s) 58, 154, 201, 475, 583, 853, 884, 1186, 1475
Ple19I CGATCG 1 cut(s) 1374
PleI GAGTC 2 cut(s) 124, 598
PpsI GAGTC 2 cut(s) 124, 598
Ppu21I YACGTR 1 cut(s) 1493
PscI ACATGT 1 cut(s) 181
PsiI TTATAA 1 cut(s) 1428
Psp6I CCWGG 3 cut(s) 220, 328, 919
PspFI CCCAGC 2 cut(s) 383, 1023
PspGI CCWGG 3 cut(s) 220, 328, 919
PspN4I GGNNCC 4 cut(s) 174, 399, 555, 1028
PspOMI GGGCCC 1 cut(s) 1026
PspPI GGNCC 4 cut(s) 554, 1026, 1027, 1049
PstI CTGCAG 2 cut(s) 478, 1089
PsuI RGATCY 2 cut(s) 1093, 1168
PvuI CGATCG 1 cut(s) 1374
PvuII CAGCTG 3 cut(s) 145, 354, 473
RsaI GTAC 5 cut(s) 180, 189, 358, 1453, 1506
RsaNI GTAC 5 cut(s) 179, 188, 357, 1452, 1505
RseI CAYNNNNRTG 3 cut(s) 318, 708, 1491
SaqAI TTAA 7 cut(s) 21, 29, 437, 462, 1014, 1116, 1367
SatI GCNGC 9 cut(s) 57, 153, 200, 474, 582, 852, 883, 1185, 1474
Sau3AI GATC 7 cut(s) 391, 543, 712, 819, 1093, 1168, 1371
Sau96I GGNCC 4 cut(s) 554, 1026, 1027, 1049
SchI GAGTC 2 cut(s) 124, 598
ScrFI CCNGG 7 cut(s) 222, 330, 761, 902, 903, 921, 1047
SduI GDGCHC 1 cut(s) 1030
SfaNI GCATC 7 cut(s) 499, 523, 818, 880, 933, 1153, 1171
SfcI CTRYAG 4 cut(s) 258, 291, 474, 1085
SinI GGWCC 1 cut(s) 554
SmaI CCCGGG 1 cut(s) 903
SmiMI CAYNNNNRTG 3 cut(s) 318, 708, 1491
SmlI CTYRAG 1 cut(s) 547
SmoI CTYRAG 1 cut(s) 547
Sse9I AATT 5 cut(s) 459, 558, 572, 956, 1444
SspMI CTAG 2 cut(s) 1106, 1241
StyD4I CCNGG 7 cut(s) 220, 328, 759, 900, 901, 919, 1045
StyI CCWWGG 1 cut(s) 566
TaaI ACNGT 6 cut(s) 292, 308, 735, 950, 1163, 1253
TaiI ACGT 1 cut(s) 1495
TaqI TCGA 4 cut(s) 213, 602, 658, 1062
TasI AATT 5 cut(s) 459, 558, 572, 956, 1444
TatI WGTACW 2 cut(s) 178, 187
TfiI GAWTC 3 cut(s) 526, 1264, 1279
Tru1I TTAA 7 cut(s) 21, 29, 437, 462, 1014, 1116, 1367
Tru9I TTAA 7 cut(s) 21, 29, 437, 462, 1014, 1116, 1367
TscAI CASTG 2 cut(s) 799, 829
TseFI GTSAC 2 cut(s) 629, 674
TseI GCWGC 9 cut(s) 56, 152, 199, 473, 581, 851, 882, 1184, 1473
Tsp45I GTSAC 2 cut(s) 629, 674
TspDTI ATGAA 4 cut(s) 231, 378, 473, 1143
TspGWI ACGGA 2 cut(s) 374, 521
TspMI CCCGGG 1 cut(s) 901
TspRI CASTG 2 cut(s) 799, 829
VpaK11BI GGWCC 1 cut(s) 554
XagI CCTNNNNNAGG 1 cut(s) 918
XapI RAATTY 3 cut(s) 572, 956, 1444
XceI RCATGY 2 cut(s) 56, 185
XcmI CCANNNNNNNNNTGG 2 cut(s) 78, 564
XmaI CCCGGG 1 cut(s) 901
XmnI GAANNNNTTC 1 cut(s) 1444
XspI CTAG 2 cut(s) 1106, 1241
Zsp2I ATGCAT 2 cut(s) 811, 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.