RchiOBHm_Chr6g0244551

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006047
Physical Location & Seq
Reverse (-)
598062 .. 598637
576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGGAGAAGGTAGTTCCAAAGCTTTTCTTCATAGCTCTGTTCACGTTCATACCATTACTAGGGCACTGTAGCAGCATCGAAGAAAATAGAGAATTTGACCAATGGTTTCAGAAGCTCGACAACTCAAAACCCAAGCTGACCCGATTCCACTTCTACTTTCACGACATCGTTTCAGGCTATGGCCAGACATCATTCGTAGTGGTTCCACCACCCAAGATCATCAGGCCATCGACCACCTTGTTCGGACAAGTCAACATGTTTGACAACCCACAAACCAAGGGACCCGAACTCACGTCCGGGCTTGTAGGCAGGGTTCAGGGGCTATACAGTTTCGCGTCCCAAGAAGAGCTCTGTCTACTTATGGCCATGACCATTGTTTTCACCAGCGGCAAACACAACGGTAGCAGTGTCACTATTTTGGGGCGCAACGCAGTGTTTCAATCCCGGCGAGAGTTTCCAATTGTCGGCGGAACTGGCGATTTCCGGTTGGCCCGTGGATTTGCGTCAGTCTCGAGCTATTTTATTAATGCTACTCTTGGTATTCTGGAATATAATCTTATGGTTATACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

191

Amino Acids

21.5

Weight (kDa)

8.93

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 47 - 188 8.9e-48 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 355
AccII CGCG 1 cut(s) 335
AciI CCGC 2 cut(s) 387, 468
AcoI YGGCCR 2 cut(s) 181, 363
AcsI RAATTY 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 59, 464
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 1 cut(s) 440
AjiI CACGTC 1 cut(s) 294
AluBI AGCT 6 cut(s) 22, 35, 115, 136, 349, 516
AluI AGCT 6 cut(s) 22, 35, 115, 136, 349, 516
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 1 cut(s) 514
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 4 cut(s) 181, 224, 363, 489
ApeKI GCWGC 1 cut(s) 72
ApoI RAATTY 1 cut(s) 92
AseI ATTAAT 1 cut(s) 525
AspLEI GCGC 1 cut(s) 426
AspS9I GGNCC 2 cut(s) 281, 490
AsuC2I CCSGG 2 cut(s) 298, 445
AsuHPI GGTGA 1 cut(s) 373
AvaI CYCGRG 1 cut(s) 511
AvaII GGWCC 1 cut(s) 281
BaeGI GKGCMC 1 cut(s) 66
BalI TGGCCA 2 cut(s) 183, 365
BanII GRGCYC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 235
BcnI CCSGG 2 cut(s) 298, 445
BcoDI GTCTC 1 cut(s) 514
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 67
BisI GCNGC 2 cut(s) 73, 388
BlsI GCNGC 2 cut(s) 74, 389
Bme1390I CCNGG 2 cut(s) 298, 445
Bme18I GGWCC 1 cut(s) 281
BmeT110I CYCGRG 1 cut(s) 511
BmgBI CACGTC 1 cut(s) 294
BmgT120I GGNCC 2 cut(s) 281, 490
BmiI GGNNCC 3 cut(s) 204, 282, 283
BmrFI CCNGG 2 cut(s) 298, 445
BmsI GCATC 1 cut(s) 84
BpuMI CCSGG 2 cut(s) 298, 445
BsaJI CCNNGG 2 cut(s) 276, 493
BsaWI WCCGGW 1 cut(s) 483
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 464
Bse1I ACTGG 1 cut(s) 478
BseDI CCNNGG 2 cut(s) 276, 493
BseLI CCNNNNNNNGG 2 cut(s) 59, 464
BseNI ACTGG 1 cut(s) 478
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 4 cut(s) 183, 226, 365, 491
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 3 cut(s) 297, 445, 484
BslFI GGGAC 2 cut(s) 294, 322
BslI CCNNNNNNNGG 2 cut(s) 59, 464
BsmAI GTCTC 1 cut(s) 514
BsmFI GGGAC 2 cut(s) 294, 322
BsnI GGCC 4 cut(s) 183, 226, 365, 491
BsoBI CYCGRG 1 cut(s) 511
Bsp1286I GDGCHC 2 cut(s) 66, 351
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 2 cut(s) 387, 468
BspANI GGCC 4 cut(s) 183, 226, 365, 491
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 3 cut(s) 204, 282, 283
BspQI GCTCTTC 1 cut(s) 339
BsrI ACTGG 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 276, 493
BssMI GATC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 276
Bst4CI ACNGT 3 cut(s) 68, 329, 401
Bst6I CTCTTC 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 493
BstFNI CGCG 1 cut(s) 335
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 514
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 1 cut(s) 474
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 2 cut(s) 296, 443
BstSFI CTRYAG 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 66
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 1 cut(s) 84
BsuRI GGCC 4 cut(s) 183, 226, 365, 491
BtgI CCRYGG 1 cut(s) 493
BtrI CACGTC 1 cut(s) 294
BtsI GCAGTG 2 cut(s) 412, 438
BtsIMutI CAGTG 3 cut(s) 64, 412, 438
CfoI GCGC 1 cut(s) 426
Cfr13I GGNCC 2 cut(s) 281, 490
CseI GACGC 2 cut(s) 324, 492
CviAII CATG 2 cut(s) 256, 367
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
EaeI YGGCCR 2 cut(s) 181, 363
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
EciI GGCGGA 1 cut(s) 483
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 511
EcoICRI GAGCTC 1 cut(s) 349
EcoO109I RGGNCCY 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 2 cut(s) 259, 370
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 2 cut(s) 294, 322
FatI CATG 2 cut(s) 255, 366
FblI GTMKAC 1 cut(s) 355
Fnu4HI GCNGC 2 cut(s) 73, 388
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 2 cut(s) 73, 388
FspBI CTAG 1 cut(s) 59
GlaI GCGC 1 cut(s) 425
GluI GCNGC 2 cut(s) 73, 388
HaeIII GGCC 4 cut(s) 183, 226, 365, 491
HapII CCGG 3 cut(s) 297, 445, 484
HgaI GACGC 2 cut(s) 324, 492
HhaI GCGC 1 cut(s) 426
Hin1II CATG 2 cut(s) 259, 370
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 1 cut(s) 144
HpaII CCGG 3 cut(s) 297, 445, 484
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 3 cut(s) 42, 253, 356
Hpy188I TCNGA 2 cut(s) 111, 245
Hpy188III TCNNGA 3 cut(s) 161, 511, 545
Hpy8I GTNNAC 3 cut(s) 42, 253, 356
HpyCH4III ACNGT 3 cut(s) 68, 329, 401
HpyCH4IV ACGT 2 cut(s) 44, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 474
HpySE526I ACGT 2 cut(s) 44, 293
Hsp92II CATG 2 cut(s) 259, 370
HspAI GCGC 1 cut(s) 424
KflI GGGWCCC 1 cut(s) 281
Kzo9I GATC 1 cut(s) 216
LguI GCTCTTC 1 cut(s) 339
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 1 cut(s) 84
MaeI CTAG 1 cut(s) 59
MaeII ACGT 2 cut(s) 44, 293
MaeIII GTNAC 1 cut(s) 409
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 3 cut(s) 19, 92, 356
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 66, 351
MlsI TGGCCA 2 cut(s) 183, 365
MluCI AATT 2 cut(s) 92, 459
MluNI TGGCCA 2 cut(s) 183, 365
Mox20I TGGCCA 2 cut(s) 183, 365
MscI TGGCCA 2 cut(s) 183, 365
MseI TTAA 1 cut(s) 525
Msp20I TGGCCA 2 cut(s) 183, 365
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 3 cut(s) 297, 445, 484
MspR9I CCNGG 2 cut(s) 298, 445
MunI CAATTG 1 cut(s) 459
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 474
NciI CCSGG 2 cut(s) 298, 445
NdeII GATC 1 cut(s) 216
NlaIII CATG 2 cut(s) 259, 370
NlaIV GGNNCC 3 cut(s) 204, 282, 283
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 511
PciI ACATGT 1 cut(s) 255
PciSI GCTCTTC 1 cut(s) 339
PfeI GAWTC 1 cut(s) 144
PkrI GCNGC 2 cut(s) 74, 389
PpuMI RGGWCCY 1 cut(s) 281
PscI ACATGT 1 cut(s) 255
PshBI ATTAAT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 281
PspN4I GGNNCC 3 cut(s) 204, 282, 283
PspPI GGNCC 2 cut(s) 281, 490
PspPPI RGGWCCY 1 cut(s) 281
PsrI GAACNNNNNNTAC 2 cut(s) 297, 329
SacI GAGCTC 1 cut(s) 351
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 2 cut(s) 73, 388
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 2 cut(s) 281, 490
ScrFI CCNGG 2 cut(s) 298, 445
SduI GDGCHC 2 cut(s) 66, 351
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 1 cut(s) 67
Sfr274I CTCGAG 1 cut(s) 511
SinI GGWCC 1 cut(s) 281
SlaI CTCGAG 1 cut(s) 511
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 2 cut(s) 92, 459
SsiI CCGC 2 cut(s) 387, 468
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 2 cut(s) 296, 443
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 3 cut(s) 68, 329, 401
TaiI ACGT 2 cut(s) 47, 296
TaqI TCGA 4 cut(s) 78, 117, 230, 512
TasI AATT 2 cut(s) 92, 459
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 3 cut(s) 71, 412, 438
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 19, 37
TspRI CASTG 3 cut(s) 71, 412, 438
VpaK11BI GGWCC 1 cut(s) 281
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 1 cut(s) 92
XceI RCATGY 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 355
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.