RchiOBHm_Chr6g0301121

Maf-like protein DDB_G0281937 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002073
Physical Location & Seq
Forward (+)
61663251 .. 61666513
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 624 bp
ATGGAAACCAATTCTTCTCCTTCGTTCAAGATAATCTTGGGCTCATCTTCGAATGCTCGCCGGAAAATTTTGGCTGAAATGGGATATGAATTCACACTCATGACTGCAGACATTGACGAAAAAAGCATCCGAAAGGAGAAACCGGAGGAGTTGGTTATGGTTCTTGCTCAGGCAAAGGCTGATGCAATCATATCAAAAATACAAACTACCCATAGTAAAGAGAAGGATGCTGAACCTGAACCAACAATTGTAATTGCCGCAGATACAGTGGTAGTCTATGAAGGTGCTGTCAGGGAAAAACCATCCAGCAAGGAAGAAGCACGGCAATTTTTGAAAGACTATTCTGGAGGACATGCAGCAACAGTGGGATCTGTGCTTGTTACAAACCTTAAAACAGGATTCAGTAAAGGAGAATGGGACCGAGTGGAGATCTATTTCCATGAAATACCAGATGAAGCCATTGAGAAGCTGATTGAGGAAGGGACGGTACTCAAAGTTGCCGGGGGTCTGATAATAGAGCATCCTCTGATTCTTCCCTTTGTCAAGGAAGTGGTAGGGACTACTGATAGTGTGATGGGACTTCCAAAAGACATAACGGAGAGACTTCTTAAAGAAGCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf

Protein Analysis

207

Amino Acids

22.77

Weight (kDa)

5.09

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf PF02545 10 - 205 8.4e-45 Maf-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 258
AclWI GGATC 1 cut(s) 376
AcsI RAATTY 2 cut(s) 66, 89
AfaI GTAC 1 cut(s) 489
AgsI TTSAA 2 cut(s) 28, 334
AluBI AGCT 2 cut(s) 469, 617
AluI AGCT 2 cut(s) 469, 617
Alw26I GTCTC 1 cut(s) 595
AlwI GGATC 1 cut(s) 376
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 66, 89
AspS9I GGNCC 1 cut(s) 418
AsuC2I CCSGG 1 cut(s) 502
AsuII TTCGAA 1 cut(s) 50
AvaII GGWCC 1 cut(s) 418
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 2 cut(s) 471, 503
BbvI GCAGC 1 cut(s) 368
BccI CCATC 2 cut(s) 310, 568
BceAI ACGGC 1 cut(s) 338
BcnI CCSGG 1 cut(s) 502
BcoDI GTCTC 1 cut(s) 595
BfmI CTRYAG 2 cut(s) 105, 620
BglII AGATCT 1 cut(s) 429
BisI GCNGC 2 cut(s) 258, 357
BlsI GCNGC 2 cut(s) 259, 358
Bme1390I CCNGG 1 cut(s) 502
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 1 cut(s) 502
BmsI GCATC 4 cut(s) 135, 172, 217, 529
BpmI CTGGAG 1 cut(s) 366
Bpu10I CCTNAGC 1 cut(s) 168
Bpu14I TTCGAA 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 502
BsaJI CCNNGG 1 cut(s) 501
BsaWI WCCGGW 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 4 cut(s) 126, 232, 302, 520
BseMII CTCAG 1 cut(s) 182
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 368
BsiSI CCGG 3 cut(s) 61, 143, 501
BslFI GGGAC 4 cut(s) 431, 496, 571, 591
BsmAI GTCTC 1 cut(s) 595
BsmFI GGGAC 4 cut(s) 431, 496, 571, 591
BsmI GAATGC 1 cut(s) 58
Bsp119I TTCGAA 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 2 cut(s) 368, 429
BspACI CCGC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 181
BspHI TCATGA 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 419
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 1 cut(s) 376
BspT104I TTCGAA 1 cut(s) 50
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 2 cut(s) 368, 429
Bst4CI ACNGT 3 cut(s) 268, 364, 487
BstBI TTCGAA 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 4 cut(s) 126, 232, 302, 520
BstKTI GATC 2 cut(s) 371, 432
BstMAI GTCTC 1 cut(s) 595
BstMBI GATC 2 cut(s) 368, 429
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 500
BstSFI CTRYAG 2 cut(s) 105, 620
BstV1I GCAGC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 368, 429
BstYI RGATCY 2 cut(s) 368, 429
BtsCI GGATG 4 cut(s) 126, 232, 302, 520
BtsIMutI CAGTG 2 cut(s) 273, 369
Cac8I GCNNGC 1 cut(s) 58
CciI TCATGA 1 cut(s) 99
Cfr13I GGNCC 1 cut(s) 418
Csp6I GTAC 1 cut(s) 488
CviAII CATG 3 cut(s) 100, 353, 440
CviJI RGCY 6 cut(s) 42, 74, 179, 458, 469, 617
CviKI_1 RGCY 6 cut(s) 42, 74, 179, 458, 469, 617
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 1 cut(s) 168
DpnI GATC 2 cut(s) 370, 431
DpnII GATC 2 cut(s) 368, 429
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 3 cut(s) 103, 356, 443
FaiI YATR 9 cut(s) 87, 101, 158, 191, 213, 279, 354, 441, 593
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FaqI GGGAC 4 cut(s) 431, 496, 571, 591
FatI CATG 3 cut(s) 99, 352, 439
Fnu4HI GCNGC 2 cut(s) 258, 357
FokI GGATG 4 cut(s) 113, 239, 289, 507
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 258, 357
GluI GCNGC 2 cut(s) 258, 357
GsuI CTGGAG 1 cut(s) 366
HapII CCGG 3 cut(s) 61, 143, 501
Hin1II CATG 3 cut(s) 103, 356, 443
HinfI GANTC 2 cut(s) 399, 529
HpaII CCGG 3 cut(s) 61, 143, 501
Hpy188I TCNGA 3 cut(s) 131, 510, 528
Hpy188III TCNNGA 3 cut(s) 28, 100, 345
HpyAV CCTTC 4 cut(s) 30, 217, 275, 473
HpyCH4III ACNGT 3 cut(s) 268, 364, 487
HpyCH4V TGCA 3 cut(s) 107, 185, 356
HpyF3I CTNAG 1 cut(s) 168
Hsp92II CATG 3 cut(s) 103, 356, 443
Kzo9I GATC 2 cut(s) 368, 429
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 4 cut(s) 135, 172, 217, 529
MaeIII GTNAC 1 cut(s) 379
MalI GATC 2 cut(s) 370, 431
MboI GATC 2 cut(s) 368, 429
MboII GAAGA 4 cut(s) 6, 39, 326, 524
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 2 cut(s) 368, 429
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 6 cut(s) 10, 66, 89, 246, 252, 326
MnlI CCTC 4 cut(s) 139, 341, 469, 534
MseI TTAA 2 cut(s) 390, 609
MslI CAYNNNNRTG 1 cut(s) 98
MspI CCGG 3 cut(s) 61, 143, 501
MspR9I CCNGG 1 cut(s) 502
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 58
NciI CCSGG 1 cut(s) 502
NdeII GATC 2 cut(s) 368, 429
NlaIII CATG 3 cut(s) 103, 356, 443
NlaIV GGNNCC 1 cut(s) 419
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 1 cut(s) 50
PagI TCATGA 1 cut(s) 99
PctI GAATGC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 399, 529
PkrI GCNGC 2 cut(s) 259, 358
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 1 cut(s) 418
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 2 cut(s) 368, 429
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 2 cut(s) 390, 609
SatI GCNGC 2 cut(s) 258, 357
Sau3AI GATC 2 cut(s) 368, 429
Sau96I GGNCC 1 cut(s) 418
ScrFI CCNGG 1 cut(s) 502
SduI GDGCHC 1 cut(s) 44
SetI ASST 5 cut(s) 238, 286, 390, 471, 619
SfaNI GCATC 4 cut(s) 135, 172, 217, 529
SfcI CTRYAG 2 cut(s) 105, 620
SfuI TTCGAA 1 cut(s) 50
SinI GGWCC 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 6 cut(s) 10, 66, 89, 246, 252, 326
SsiI CCGC 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 500
TaaI ACNGT 3 cut(s) 268, 364, 487
TaqI TCGA 1 cut(s) 50
TaqII GACCGA 1 cut(s) 435
TasI AATT 6 cut(s) 10, 66, 89, 246, 252, 326
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 2 cut(s) 399, 529
Tru1I TTAA 2 cut(s) 390, 609
Tru9I TTAA 2 cut(s) 390, 609
TscAI CASTG 2 cut(s) 273, 369
TseI GCWGC 1 cut(s) 356
TspDTI ATGAA 4 cut(s) 102, 294, 456, 468
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 2 cut(s) 273, 369
VpaK11BI GGWCC 1 cut(s) 418
XapI RAATTY 2 cut(s) 66, 89
XceI RCATGY 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.