RchiOBHm_Chr3g0466621

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004926
Physical Location & Seq
Forward (+)
13038071 .. 13041027
2957 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2046 bp
ATGGGCAACGCCATCGCAAAGGTCTCTCACTGCTGCTCTGTCGCCGGCGATGTCTCCAGGCGCCACGACGTCGACGTCGTCTCCCCCGACGAGGGTCTCGGCCACTCCTTCTACTACATTCGCCGCCCAGAAATAATCTCCCCCACGACCTCGTTTGAGGCTTCCTCTGAGGAGTTCCCCAACCCGGCTACCACCACGTTCCGGTCAATCTCCGGCGCCTCCGTCAGCGCCAACACGTTCACGCCGCCGTCTACTACACTATATGATCTCTACCCCGACAGCTCCGGTGGTCTCGATCGTGCTGCTAGGTTTGAGAGCTCTACTTCGTTCGCTTCGATTCCTCTGCAGCCGGTTCCTCGGAACCCGGGACTTCCGGGGTCCGGTCCTGCTGAAAGAGGCTTCTTGTCCGGTCCGATCGAGCGGGGGTTCATGTCCGGTCCGATTGACCGGTTGGTGTGTTCGGGTCCGATTGAGAAGCAGTCCGAGAAGATGGAGAGAAGCCTCTCTTTTGGGCTTGAGAAAAAGCCCAAGAGAAGGAATTTGATGAGGATGATAAGAAGAGCGATTTCGAAGAGTATGAATCGGCGGCACAAGTCGGCTGTGACGCCGAAAGAAACGGAGTCGGAGAAAATCGGCGAGGAGTTGAATCCGAGTAGTACTAACTTGAGCAGCCATGCGAGTTTGAGTGGAAATGAGAATGGGATTGGGGATTTCTCAATGGAAAGTCAGAATCTTCAGTGGGCTCAAGGGAAGGCGGGTGAGGATCGAGTTCATGTTGTGGTTTCTGATGAGCATGGTTGGGTGTTCGTGGGGATTTATGATGGGTTTAATGGCCCTGATGCTCCTGATTATTTGTTGTCTAATCTTTACTCTGCTGTGCATGAGGAGCTCAAGGGATTGCTGTGGAGTGACATGTTGGAAATCGAATCCATGGATTTGAAATCATCGGAGTCGAATGTGGTTTCGTGTTCTTTTAGTGAGGTTGGCTGCATTGATGGAGTTGATAAGGAGAATTGTACAGTGGAGAATGTGTTGTCAAATAGAAAACAGAGCAGGCATTTGAGGAATGAAAGTAATGAGATGGATAATAAGTGGGAGGAGAGTCAGAGGAGATGGAAGTGTGAATGGGATAGAGAAAGGCCAGAGCTTGATAGGTTAACTAAGGAGAACTTGAAGCTTAAGGTGTCAAATGGTGTGGCAGCTGCGAACCCCTCGGATGTTCTCAAGGCTCTTTCGGAGGCATTAAGAAAAACTGAAGAGGCCTATTTGGATGTTGCGGATAAGATGGTTACGGAAAATCCTGAGCTGGCTCTGATGGGGTCTTGTGTGCTGGTGATGTTGATGAAGGGAGATGATGTTTATCTGATGAATGTTGGGGATAGTCGTGCGGTTTTAGCTCAGAAAGCCGATCCCAAGATCAAGCGAAACTTGGAACGGATTAATGAGGAAGCTTTATGCGAACGTCGATCATCTAGTGCTGATGAGCTTTACAGTTTAAACAATTTGACTTCGGTTCAGCTGACAATGGATCACAGCACAAGTGTGAAAGAGGAAGTTCGAAGAATAAGGAATGAACATCCAGATGATGCTACTGCAGTGATGAATAAGAGAGTGAAGGGTTATTTGAAGGTCACTCGGGCATTTGGTGCCGGCTTTCTAAAACAGCCAAAATGGAATGATGCACTGCTAGAGATGTTTAGACTCAATTATGTTGGGACTGCCCCCTACATTACATGTATACCATCACTGTATCACCACAAACTCAGACCAAGAGACAGACTCTTAATATTGTCCTCTGATGGACTCTATCAGTACTTCACCAATGAAGAAGCCGTCTCCGAAGTAGAGTTGTTCATCTCTTCCTTTCCTGATGGTGACCCTGCACAACATCTGATTGAAGAAGTGTTGTTTAGAGCAGCAAAGAAAGCTGGACTGGCCTTCCATGAACTGCTTGACATCCCACAAGGCGAACGTCGTCGGTACCATGATGACATTTCCATTCTTATTATTTCACTTGAGGGAAGAATATGGCGTTCATCGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

681

Amino Acids

76.18

Weight (kDa)

5.67

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 403 - 623 2.1e-27 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 2 cut(s) 72, 78
Acc65I GGTACC 1 cut(s) 1980
AccB1I GGYRCC 4 cut(s) 60, 215, 1646, 1980
AccBSI CCGCTC 1 cut(s) 421
AccI GTMKAC 3 cut(s) 72, 251, 1738
AciI CCGC 7 cut(s) 124, 245, 421, 586, 755, 1277, 1388
AclWI GGATC 3 cut(s) 771, 1403, 1536
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 2 cut(s) 719, 1275
AcyI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
AfaI GTAC 4 cut(s) 658, 1018, 1814, 1982
AfiI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
AflII CTTAAG 1 cut(s) 1178
AflIII ACRYGT 3 cut(s) 234, 912, 1733
AgeI ACCGGT 1 cut(s) 447
AgsI TTSAA 5 cut(s) 646, 940, 1174, 1627, 1898
AjnI CCWGG 1 cut(s) 56
AleI CACNNNNGTG 1 cut(s) 1541
Alw21I GWGCWC 2 cut(s) 320, 891
Alw26I GTCTC 7 cut(s) 28, 58, 85, 101, 296, 1767, 1840
AlwI GGATC 3 cut(s) 771, 1403, 1536
Ama87I CYCGRG 2 cut(s) 364, 1635
AoxI GGCC 5 cut(s) 100, 832, 1139, 1260, 1935
ApeKI GCWGC 8 cut(s) 33, 302, 346, 669, 987, 1199, 1202, 1916
ApoI RAATTY 1 cut(s) 538
AseI ATTAAT 1 cut(s) 1440
AsiGI ACCGGT 1 cut(s) 447
Asp718I GGTACC 1 cut(s) 1980
AspLEI GCGC 3 cut(s) 63, 218, 230
AspS9I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
AsuC2I CCSGG 4 cut(s) 185, 365, 366, 375
AsuHPI GGTGA 5 cut(s) 770, 1345, 1745, 1810, 1886
AsuII TTCGAA 2 cut(s) 569, 1558
AvaI CYCGRG 2 cut(s) 364, 1635
AvaII GGWCC 5 cut(s) 378, 383, 410, 437, 464
BanI GGYRCC 4 cut(s) 60, 215, 1646, 1980
BanII GRGCYC 3 cut(s) 320, 745, 891
Bbv12I GWGCWC 2 cut(s) 320, 891
BbvI GCAGC 8 cut(s) 20, 289, 358, 681, 974, 1189, 1211, 1928
BceAI ACGGC 2 cut(s) 232, 1817
BcgI CGANNNNNNTGC 5 cut(s) 29, 284, 318, 325, 359
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 4 cut(s) 185, 365, 366, 375
BcoDI GTCTC 7 cut(s) 28, 58, 85, 101, 296, 1767, 1840
BfaI CTAG 3 cut(s) 306, 1473, 1688
BfmI CTRYAG 2 cut(s) 344, 1593
BfoI RGCGCY 3 cut(s) 64, 219, 231
BfrI CTTAAG 1 cut(s) 1178
BmcAI AGTACT 2 cut(s) 658, 1814
Bme1390I CCNGG 5 cut(s) 58, 185, 365, 366, 375
Bme18I GGWCC 5 cut(s) 378, 383, 410, 437, 464
BmeT110I CYCGRG 2 cut(s) 364, 1635
BmgT120I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
BmiI GGNNCC 8 cut(s) 62, 217, 354, 362, 379, 465, 1648, 1982
BmrFI CCNGG 5 cut(s) 58, 185, 365, 366, 375
BmsI GCATC 3 cut(s) 829, 1576, 1669
BoxI GACNNNNGTC 1 cut(s) 93
BplI GAGNNNNNCTC 4 cut(s) 149, 181, 1764, 1796
BpmI CTGGAG 1 cut(s) 40
Bpu10I CCTNAGC 1 cut(s) 1302
Bpu14I TTCGAA 2 cut(s) 569, 1558
BpuEI CTTGAG 6 cut(s) 536, 685, 729, 875, 1208, 2036
BpuMI CCSGG 4 cut(s) 185, 365, 366, 375
BsaBI GATNNNNATC 1 cut(s) 1359
BsaHI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
BsaI GGTCTC 3 cut(s) 28, 101, 296
BsaJI CCNNGG 5 cut(s) 356, 364, 374, 930, 1212
BsaWI WCCGGW 6 cut(s) 201, 284, 380, 407, 434, 447
BsaXI ACNNNNNCTCC 6 cut(s) 65, 95, 1016, 1046, 1341, 1371
Bsc4I CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
Bse118I RCCGGY 4 cut(s) 44, 349, 447, 1649
Bse1I ACTGG 1 cut(s) 1938
Bse8I GATNNNNATC 1 cut(s) 1359
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 5 cut(s) 356, 364, 374, 930, 1212
BseGI GGATG 5 cut(s) 555, 1222, 1276, 1576, 1956
BseJI GATNNNNATC 1 cut(s) 1359
BseLI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
BseMII CTCAG 4 cut(s) 159, 1293, 1412, 1777
BseNI ACTGG 1 cut(s) 1938
BseRI GAGGAG 5 cut(s) 185, 653, 899, 1112, 1123
BseXI GCAGC 8 cut(s) 20, 289, 358, 681, 974, 1189, 1211, 1928
BsgI GTGCAG 1 cut(s) 1866
Bsh1285I CGRYCG 2 cut(s) 298, 417
BshFI GGCC 5 cut(s) 102, 834, 1141, 1262, 1937
BshNI GGYRCC 4 cut(s) 60, 215, 1646, 1980
BshTI ACCGGT 1 cut(s) 447
BsiEI CGRYCG 2 cut(s) 298, 417
BsiHKAI GWGCWC 2 cut(s) 320, 891
BsiHKCI CYCGRG 2 cut(s) 364, 1635
BslFI GGGAC 2 cut(s) 381, 1729
BslI CCNNNNNNNGG 6 cut(s) 91, 92, 184, 201, 380, 534
BsmAI GTCTC 7 cut(s) 28, 58, 85, 101, 296, 1767, 1840
BsmBI CGTCTC 2 cut(s) 85, 1840
BsmFI GGGAC 2 cut(s) 381, 1729
BsnI GGCC 5 cut(s) 102, 834, 1141, 1262, 1937
Bso31I GGTCTC 3 cut(s) 28, 101, 296
BsoBI CYCGRG 2 cut(s) 364, 1635
Bsp119I TTCGAA 2 cut(s) 569, 1558
Bsp1286I GDGCHC 3 cut(s) 320, 745, 891
Bsp1407I TGTACA 1 cut(s) 1016
Bsp143I GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
Bsp19I CCATGG 1 cut(s) 930
BspACI CCGC 7 cut(s) 124, 245, 421, 586, 755, 1277, 1388
BspANI GGCC 5 cut(s) 102, 834, 1141, 1262, 1937
BspCNI CTCAG 4 cut(s) 160, 1294, 1411, 1776
BspLI GGNNCC 8 cut(s) 62, 217, 354, 362, 379, 465, 1648, 1982
BspMAI CTGCAG 2 cut(s) 348, 1597
BspPI GGATC 3 cut(s) 771, 1403, 1536
BspQI GCTCTTC 1 cut(s) 553
BspT104I TTCGAA 2 cut(s) 569, 1558
BspT107I GGYRCC 4 cut(s) 60, 215, 1646, 1980
BspTI CTTAAG 1 cut(s) 1178
BspTNI GGTCTC 3 cut(s) 28, 101, 296
BsrBI CCGCTC 1 cut(s) 421
BsrFI RCCGGY 4 cut(s) 44, 349, 447, 1649
BsrGI TGTACA 1 cut(s) 1016
BsrI ACTGG 1 cut(s) 1938
BssAI RCCGGY 4 cut(s) 44, 349, 447, 1649
BssECI CCNNGG 5 cut(s) 356, 364, 374, 930, 1212
BssMI GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
BssNAI GTATAC 1 cut(s) 1739
BssNI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
BssT1I CCWWGG 1 cut(s) 930
Bst1107I GTATAC 1 cut(s) 1739
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 3 cut(s) 1021, 1493, 1749
Bst6I CTCTTC 4 cut(s) 553, 566, 1251, 1864
BstACI GRCGYC 5 cut(s) 61, 69, 75, 216, 605
BstAFI CTTAAG 1 cut(s) 1178
BstAPI GCANNNNNTGC 1 cut(s) 1646
BstAUI TGTACA 1 cut(s) 1016
BstBI TTCGAA 2 cut(s) 569, 1558
BstC8I GCNNGC 4 cut(s) 46, 1055, 1308, 1651
BstDEI CTNAG 5 cut(s) 168, 1161, 1302, 1398, 1763
BstDSI CCRYGG 1 cut(s) 930
BstEII GGTNACC 1 cut(s) 1874
BstF5I GGATG 5 cut(s) 555, 1222, 1276, 1576, 1956
BstH2I RGCGCY 3 cut(s) 64, 219, 231
BstHHI GCGC 3 cut(s) 63, 218, 230
BstKTI GATC 8 cut(s) 268, 298, 417, 766, 1411, 1419, 1469, 1531
BstMAI GTCTC 7 cut(s) 28, 58, 85, 101, 296, 1767, 1840
BstMBI GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
BstMCI CGRYCG 2 cut(s) 298, 417
BstMWI GCNNNNNNNGC 7 cut(s) 886, 1394, 1403, 1646, 1925, 1934, 2038
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 2 cut(s) 916, 1737
BstPAI GACNNNNGTC 1 cut(s) 93
BstPI GGTNACC 1 cut(s) 1874
BstSCI CCNGG 5 cut(s) 56, 183, 363, 364, 373
BstSFI CTRYAG 2 cut(s) 344, 1593
BstV1I GCAGC 8 cut(s) 20, 289, 358, 681, 974, 1189, 1211, 1928
BstZ17I GTATAC 1 cut(s) 1739
BsuRI GGCC 5 cut(s) 102, 834, 1141, 1262, 1937
BtgI CCRYGG 1 cut(s) 930
BtgZI GCGATG 2 cut(s) 63, 2022
BtsCI GGATG 5 cut(s) 555, 1222, 1276, 1576, 1956
BtsI GCAGTG 3 cut(s) 28, 1602, 1682
BtsIMutI CAGTG 6 cut(s) 28, 743, 1026, 1602, 1682, 1745
Cac8I GCNNGC 4 cut(s) 46, 1055, 1308, 1651
CfoI GCGC 3 cut(s) 63, 218, 230
Cfr10I RCCGGY 4 cut(s) 44, 349, 447, 1649
Cfr13I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
Cfr9I CCCGGG 1 cut(s) 364
CpoI CGGWCCG 2 cut(s) 410, 437
CseI GACGC 1 cut(s) 613
Csp6I GTAC 4 cut(s) 657, 1017, 1813, 1981
CspAI ACCGGT 1 cut(s) 447
CspCI CAANNNNNGTGG 2 cut(s) 1176, 1211
CspI CGGWCCG 2 cut(s) 410, 437
CviQI GTAC 4 cut(s) 657, 1017, 1813, 1981
DdeI CTNAG 5 cut(s) 168, 1161, 1302, 1398, 1763
DinI GGCGCC 2 cut(s) 62, 217
DpnI GATC 8 cut(s) 267, 297, 416, 765, 1410, 1418, 1468, 1530
DpnII GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
DraI TTTAAA 1 cut(s) 1497
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 4 cut(s) 553, 566, 1251, 1864
EarI CTCTTC 4 cut(s) 553, 566, 1251, 1864
Ecl136II GAGCTC 2 cut(s) 318, 889
Eco130I CCWWGG 1 cut(s) 930
Eco147I AGGCCT 1 cut(s) 1262
Eco24I GRGCYC 3 cut(s) 320, 745, 891
Eco31I GGTCTC 3 cut(s) 28, 101, 296
Eco47I GGWCC 5 cut(s) 378, 383, 410, 437, 464
Eco53kI GAGCTC 2 cut(s) 318, 889
Eco57I CTGAAG 2 cut(s) 719, 1275
Eco88I CYCGRG 2 cut(s) 364, 1635
Eco91I GGTNACC 1 cut(s) 1874
EcoICRI GAGCTC 2 cut(s) 318, 889
EcoO65I GGTNACC 1 cut(s) 1874
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 930
EcoT38I GRGCYC 3 cut(s) 320, 745, 891
EgeI GGCGCC 2 cut(s) 62, 217
EheI GGCGCC 2 cut(s) 62, 217
ErhI CCWWGG 1 cut(s) 930
Esp3I CGTCTC 2 cut(s) 85, 1840
FalI AAGNNNNNCTT 6 cut(s) 490, 522, 1154, 1186, 1412, 1444
FaqI GGGAC 2 cut(s) 381, 1729
FauI CCCGC 2 cut(s) 414, 748
FblI GTMKAC 3 cut(s) 72, 251, 1738
FokI GGATG 5 cut(s) 562, 1229, 1283, 1563, 1943
FriOI GRGCYC 3 cut(s) 320, 745, 891
FspBI CTAG 3 cut(s) 306, 1473, 1688
GlaI GCGC 3 cut(s) 62, 217, 229
GsuI CTGGAG 1 cut(s) 40
HaeII RGCGCY 3 cut(s) 64, 219, 231
HaeIII GGCC 5 cut(s) 102, 834, 1141, 1262, 1937
HgaI GACGC 1 cut(s) 613
HhaI GCGC 3 cut(s) 63, 218, 230
Hin1I GRCGYC 5 cut(s) 61, 69, 75, 216, 605
Hin6I GCGC 3 cut(s) 61, 216, 228
HinP1I GCGC 3 cut(s) 61, 216, 228
HincII GTYRAC 2 cut(s) 73, 1158
HindII GTYRAC 2 cut(s) 73, 1158
HindIII AAGCTT 2 cut(s) 1175, 1449
HpaI GTTAAC 1 cut(s) 1158
HphI GGTGA 5 cut(s) 770, 1345, 1745, 1810, 1886
Hpy166II GTNNAC 5 cut(s) 73, 240, 252, 1158, 1739
Hpy188III TCNNGA 5 cut(s) 293, 845, 1301, 1580, 1868
Hpy8I GTNNAC 5 cut(s) 73, 240, 252, 1158, 1739
Hpy99I CGWCG 8 cut(s) 71, 74, 77, 80, 92, 1467, 1977, 1980
HpyAV CCTTC 7 cut(s) 118, 528, 745, 1339, 1609, 1621, 1948
HpyCH4III ACNGT 3 cut(s) 1021, 1493, 1749
HpyCH4IV ACGT 6 cut(s) 69, 75, 197, 236, 1462, 1972
HpyCH4V TGCA 6 cut(s) 346, 880, 990, 1595, 1682, 1883
HpyF10VI GCNNNNNNNGC 7 cut(s) 886, 1394, 1403, 1646, 1925, 1934, 2038
HpyF3I CTNAG 5 cut(s) 168, 1161, 1302, 1398, 1763
HpySE526I ACGT 6 cut(s) 69, 75, 197, 236, 1462, 1972
Hsp92I GRCGYC 5 cut(s) 61, 69, 75, 216, 605
HspAI GCGC 3 cut(s) 61, 216, 228
KasI GGCGCC 2 cut(s) 60, 215
KpnI GGTACC 1 cut(s) 1984
KroI GCCGGC 2 cut(s) 44, 1649
KroNI GCCGGC 2 cut(s) 46, 1651
KspAI GTTAAC 1 cut(s) 1158
Kzo9I GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
LguI GCTCTTC 1 cut(s) 553
LmnI GCTCC 3 cut(s) 287, 847, 886
Lsp1109I GCAGC 8 cut(s) 20, 289, 358, 681, 974, 1189, 1211, 1928
LweI GCATC 3 cut(s) 829, 1576, 1669
MaeI CTAG 3 cut(s) 306, 1473, 1688
MaeII ACGT 6 cut(s) 69, 75, 197, 236, 1462, 1972
MaeIII GTNAC 5 cut(s) 601, 908, 1288, 1630, 1874
MalI GATC 8 cut(s) 267, 297, 416, 765, 1410, 1418, 1468, 1530
MbiI CCGCTC 1 cut(s) 421
MboI GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
MhlI GDGCHC 3 cut(s) 320, 745, 891
MluCI AATT 4 cut(s) 538, 1012, 1501, 1705
Mly113I GGCGCC 2 cut(s) 61, 216
MlyI GAGTC 6 cut(s) 629, 959, 1111, 1695, 1773, 1797
MmeI TCCRAC 2 cut(s) 603, 897
MreI CGCCGGCG 1 cut(s) 44
MroNI GCCGGC 2 cut(s) 44, 1649
MseI TTAA 7 cut(s) 828, 1157, 1179, 1244, 1440, 1496, 1784
MslI CAYNNNNRTG 2 cut(s) 1541, 1581
MspA1I CMGCKG 2 cut(s) 1202, 1519
MspCI CTTAAG 1 cut(s) 1178
MspR9I CCNGG 5 cut(s) 58, 185, 365, 366, 375
MssI GTTTAAAC 1 cut(s) 1497
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 7 cut(s) 886, 1394, 1403, 1646, 1925, 1934, 2038
NaeI GCCGGC 2 cut(s) 46, 1651
NarI GGCGCC 2 cut(s) 61, 216
NciI CCSGG 4 cut(s) 185, 365, 366, 375
NcoI CCATGG 1 cut(s) 930
NdeII GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
NgoMIV GCCGGC 2 cut(s) 44, 1649
NlaIV GGNNCC 8 cut(s) 62, 217, 354, 362, 379, 465, 1648, 1982
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 4 cut(s) 601, 908, 1630, 1874
NspI RCATGY 2 cut(s) 916, 1737
NspV TTCGAA 2 cut(s) 569, 1558
OliI CACNNNNGTG 1 cut(s) 1541
PceI AGGCCT 1 cut(s) 1262
PciI ACATGT 2 cut(s) 912, 1733
PciSI GCTCTTC 1 cut(s) 553
PcsI WCGNNNNNNNCGW 4 cut(s) 72, 75, 84, 332
PdiI GCCGGC 2 cut(s) 46, 1651
PfeI GAWTC 5 cut(s) 337, 580, 646, 730, 926
PflFI GACNNNGTC 1 cut(s) 77
PinAI ACCGGT 1 cut(s) 447
Ple19I CGATCG 2 cut(s) 298, 417
PleI GAGTC 6 cut(s) 628, 958, 1110, 1695, 1773, 1797
PluTI GGCGCC 2 cut(s) 64, 219
PmeI GTTTAAAC 1 cut(s) 1497
PpsI GAGTC 6 cut(s) 628, 958, 1110, 1695, 1773, 1797
PscI ACATGT 2 cut(s) 912, 1733
PshAI GACNNNNGTC 1 cut(s) 93
PshBI ATTAAT 1 cut(s) 1440
Psp124BI GAGCTC 2 cut(s) 320, 891
Psp6I CCWGG 1 cut(s) 56
PspEI GGTNACC 1 cut(s) 1874
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 8 cut(s) 62, 217, 354, 362, 379, 465, 1648, 1982
PspPI GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
PstI CTGCAG 2 cut(s) 348, 1597
PsyI GACNNNGTC 1 cut(s) 77
PvuI CGATCG 2 cut(s) 298, 417
PvuII CAGCTG 2 cut(s) 1202, 1519
RsaI GTAC 4 cut(s) 658, 1018, 1814, 1982
RsaNI GTAC 4 cut(s) 657, 1017, 1813, 1981
RseI CAYNNNNRTG 2 cut(s) 1541, 1581
Rsr2I CGGWCCG 2 cut(s) 410, 437
RsrII CGGWCCG 2 cut(s) 410, 437
SacI GAGCTC 2 cut(s) 320, 891
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 553
SaqAI TTAA 7 cut(s) 828, 1157, 1179, 1244, 1440, 1496, 1784
Sau3AI GATC 8 cut(s) 265, 295, 414, 763, 1408, 1416, 1466, 1528
Sau96I GGNCC 6 cut(s) 378, 383, 410, 437, 464, 833
ScaI AGTACT 2 cut(s) 658, 1814
SchI GAGTC 6 cut(s) 629, 959, 1111, 1695, 1773, 1797
ScrFI CCNGG 5 cut(s) 58, 185, 365, 366, 375
SduI GDGCHC 3 cut(s) 320, 745, 891
SfaNI GCATC 3 cut(s) 829, 1576, 1669
SfcI CTRYAG 2 cut(s) 344, 1593
SfoI GGCGCC 2 cut(s) 62, 217
SfuI TTCGAA 2 cut(s) 569, 1558
SgrAI CRCCGGYG 1 cut(s) 44
SgrDI CGTCGACG 1 cut(s) 71
SinI GGWCC 5 cut(s) 378, 383, 410, 437, 464
SmaI CCCGGG 1 cut(s) 366
SmiMI CAYNNNNRTG 2 cut(s) 1541, 1581
SmlI CTYRAG 7 cut(s) 515, 664, 744, 890, 1178, 1223, 2015
SmoI CTYRAG 7 cut(s) 515, 664, 744, 890, 1178, 1223, 2015
Sse9I AATT 4 cut(s) 538, 1012, 1501, 1705
SseBI AGGCCT 1 cut(s) 1262
SsiI CCGC 7 cut(s) 124, 245, 421, 586, 755, 1277, 1388
SspDI GGCGCC 2 cut(s) 60, 215
SspI AATATT 1 cut(s) 1788
SspMI CTAG 3 cut(s) 306, 1473, 1688
SstI GAGCTC 2 cut(s) 320, 891
StuI AGGCCT 1 cut(s) 1262
StyD4I CCNGG 5 cut(s) 56, 183, 363, 364, 373
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 3 cut(s) 1021, 1493, 1749
TaiI ACGT 6 cut(s) 72, 78, 200, 239, 1465, 1975
TasI AATT 4 cut(s) 538, 1012, 1501, 1705
TatI WGTACW 3 cut(s) 656, 1016, 1812
TauI GCSGC 3 cut(s) 126, 247, 589
TfiI GAWTC 5 cut(s) 337, 580, 646, 730, 926
Tru1I TTAA 7 cut(s) 828, 1157, 1179, 1244, 1440, 1496, 1784
Tru9I TTAA 7 cut(s) 828, 1157, 1179, 1244, 1440, 1496, 1784
TscAI CASTG 6 cut(s) 35, 743, 1026, 1602, 1689, 1752
TseFI GTSAC 4 cut(s) 601, 908, 1630, 1874
TseI GCWGC 8 cut(s) 33, 302, 346, 669, 987, 1199, 1202, 1916
Tsp45I GTSAC 4 cut(s) 601, 908, 1630, 1874
TspGWI ACGGA 4 cut(s) 211, 632, 1307, 1450
TspMI CCCGGG 1 cut(s) 364
TspRI CASTG 6 cut(s) 35, 743, 1026, 1602, 1689, 1752
Tth111I GACNNNGTC 1 cut(s) 77
Vha464I CTTAAG 1 cut(s) 1178
VpaK11BI GGWCC 5 cut(s) 378, 383, 410, 437, 464
VspI ATTAAT 1 cut(s) 1440
XapI RAATTY 1 cut(s) 538
XceI RCATGY 2 cut(s) 916, 1737
XmaI CCCGGG 1 cut(s) 364
XmiI GTMKAC 3 cut(s) 72, 251, 1738
XspI CTAG 3 cut(s) 306, 1473, 1688
ZraI GACGTC 2 cut(s) 70, 76
ZrmI AGTACT 2 cut(s) 658, 1814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.