RchiOBHm_Chr1g0313661

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003709
Physical Location & Seq
Forward (+)
769726 .. 776342
6617 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1566 bp
ATGGGTGGTCGTCCTCTGCTCTTCTTCATCTTGATCTTTATCTGCCTCATACTCACTGCTCTTGTTGGCTCATCAGCAAGCAATGAGGCGGCTGCTGCTGCTGCTGCTGCTCCTCGTCATTATTCGATTCCATTCAATAGAAGTAGCTTCCCAGCTAGTTTCATCTTCGGAGCTGGTTCTGCTGCTTATCAGACTGAAGGAGCAGCACATATCCATGGAAAGGGACCAAGCATATGGGACACTTTCACTACAAAACATCAAGAAAAAATAACTGATAGTAGCAACGGGGATGTAGCAGATGACTTTTACCATCGTTATGAGGAAGATGTGAAATTGATGAAAAAAGTCGGTTTAGACTCCTTCAGATTTTCAATCTCATGGTCAAGATTACTACCTAAGGGAAAAGTCAAAGGTGGAGTGAACCAAGAAGGCGTAAAATTCTACAACAATCTTATAAATGAGCTCTTGTCGAATGGAATAATACCTTTTGTGACGCTTTTCCACTGGGATCTACCACAGGCTCTTGAAGATGAGTATGGTGGATTTTTAAATCATAAGATTGTTGGTGATTTTCAAGATTATGCCGATTTTTGTTTCAAAACATTTGGTGACCGAGTTAAGCATTGGATCACTCTGAACGAGCCAGTTACATACTGTGTCAACGGGTACAATGGTGGAACCAATGCACCTGGTCGATGCTCTAACTATGTCGGAAATTGCACCGCCGGTAACTCTGCTACAGAGCCTTACATTGTAGGCCATCATCTGCTGCTTGCTCACGCGCACTCTGCAAATTTATACAAGAAGAAGTATCAGGCTTCTCAAAAAGGGCAAATCGGGATTTCGGTTGTGACATACTGGTATTTAGCAAAATCGGAAACTGCTGCAAGCAAGAGGGCAGCTTCTAGGTCTCTTGATTTTGTTTTTGGATGGTTTGCTCATCCCGTTACATTCGGTGACTATCCTCAAACCATGAGAGCTTCTGTGGGTGATCGGTTGCCTAAATTCAGTAAAGAAGAAACTAAGCTCCTAAAAGGATCTCTAGATTTCCTTGGTGTCAATTACTACACAAGTAGTTATGCAGATGTTTCCTCACTCTCCACTAGTAATGTCAACCAAAGTTTCTTCGGAGATATGCATACTTCTCTCTCTACTGAGAAAAATGGGGTTCCTTTGGGTTCAGCGACTGCTTTGAGCTGGCTTTACATCTATCCAAAGGGAATTCGAGAGCTTATGTTATACATAAAAGAAAAGTACAATGATCCCGAGATTTACATTACTGAAAATGGTGTTGGTGAGGCAAATAATAGCTCCCAGCCCCTCAACGATGCCCTCAAGGGTAGTACAAGGATAAGATACCATTCTCTCCATCTTTCATATCTTTTGAAGTCCATCAAGGAGGGGGTGAAGGTGAGGGGATACTTCGCATGGTCATTTCTTGATGTTTATGAATGGGATGCAGGCTACACAGTTCGATTTGGACTCACCTACGTCGATTACAAGAATAACTTGAAGAGATATCTCAAGTACTCTGCATACTGGTTCAAGATGTTCCTCCTCAAATGA

Protein Analysis

521

Amino Acids

58.4

Weight (kDa)

8.87

Isoelectric Point (pI)

25.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 48 - 518 1.8e-154 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AccII CGCG 1 cut(s) 782
AciI CCGC 2 cut(s) 89, 723
AclWI GGATC 4 cut(s) 516, 635, 1045, 1256
AcsI RAATTY 4 cut(s) 437, 793, 1004, 1221
AcuI CTGAAG 2 cut(s) 216, 346
AfaI GTAC 4 cut(s) 668, 1256, 1345, 1529
AfiI CCNNNNNNNGG 1 cut(s) 220
AgsI TTSAA 8 cut(s) 136, 372, 527, 575, 598, 1387, 1513, 1546
AhlI ACTAGT 1 cut(s) 1103
AjnI CCWGG 1 cut(s) 688
AjuI GAANNNNNNNTTGG 2 cut(s) 1275, 1307
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 1 cut(s) 915
AlwI GGATC 4 cut(s) 516, 635, 1045, 1256
AlwNI CAGNNNCTG 1 cut(s) 1187
Ama87I CYCGRG 1 cut(s) 1265
AoxI GGCC 1 cut(s) 757
ApoI RAATTY 4 cut(s) 437, 793, 1004, 1221
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 1 cut(s) 224
AsuHPI GGTGA 8 cut(s) 578, 620, 968, 1001, 1307, 1417, 1423, 1477
AvaI CYCGRG 1 cut(s) 1265
AvaII GGWCC 1 cut(s) 224
AxyI CCTNAGG 1 cut(s) 396
BaeI ACNNNNGTAYC 2 cut(s) 658, 691
BanII GRGCYC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 465
BccI CCATC 5 cut(s) 318, 768, 924, 1377, 1400
BciT130I CCWGG 1 cut(s) 690
BciVI GTATCC 1 cut(s) 1412
BcoDI GTCTC 1 cut(s) 915
BcuI ACTAGT 1 cut(s) 1103
BfaI CTAG 4 cut(s) 156, 906, 1043, 1104
BfmI CTRYAG 1 cut(s) 738
BfuI GTATCC 1 cut(s) 1412
BmcAI AGTACT 1 cut(s) 1529
Bme1390I CCNGG 1 cut(s) 690
Bme18I GGWCC 1 cut(s) 224
BmeT110I CYCGRG 1 cut(s) 1265
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 3 cut(s) 225, 679, 1170
BmrFI CCNGG 1 cut(s) 690
BmrI ACTGGG 1 cut(s) 514
BmsI GCATC 3 cut(s) 686, 1318, 1447
BmuI ACTGGG 1 cut(s) 514
BpuEI CTTGAG 2 cut(s) 1319, 1508
BsaBI GATNNNNATC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 2 cut(s) 214, 1051
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse118I RCCGGY 1 cut(s) 725
Bse1I ACTGG 4 cut(s) 509, 644, 863, 1544
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 1 cut(s) 690
BseDI CCNNGG 2 cut(s) 214, 1051
BseGI GGATG 4 cut(s) 295, 935, 940, 1462
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 1146
BseNI ACTGG 4 cut(s) 509, 644, 863, 1544
BseRI GAGGAG 2 cut(s) 102, 1547
BseYI CCCAGC 2 cut(s) 151, 1314
Bsh1236I CGCG 1 cut(s) 782
BshFI GGCC 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 465
BsiHKCI CYCGRG 1 cut(s) 1265
BsiSI CCGG 1 cut(s) 726
BslFI GGGAC 2 cut(s) 237, 251
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 1 cut(s) 915
BsmFI GGGAC 2 cut(s) 237, 251
BsnI GGCC 1 cut(s) 759
Bso31I GGTCTC 1 cut(s) 915
BsoBI CYCGRG 1 cut(s) 1265
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 6 cut(s) 33, 508, 627, 991, 1037, 1261
Bsp19I CCATGG 1 cut(s) 214
BspACI CCGC 2 cut(s) 89, 723
BspANI GGCC 1 cut(s) 759
BspCNI CTCAG 1 cut(s) 1147
BspFNI CGCG 1 cut(s) 782
BspLI GGNNCC 3 cut(s) 225, 679, 1170
BspPI GGATC 4 cut(s) 516, 635, 1045, 1256
BspQI GCTCTTC 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 915
BsrDI GCAATG 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 725
BsrI ACTGG 4 cut(s) 509, 644, 863, 1544
BssAI RCCGGY 1 cut(s) 725
BssECI CCNNGG 2 cut(s) 214, 1051
BssMI GATC 6 cut(s) 33, 508, 627, 991, 1037, 1261
BssT1I CCWWGG 2 cut(s) 214, 1051
Bst2UI CCWGG 1 cut(s) 690
Bst4CI ACNGT 2 cut(s) 656, 1471
Bst6I CTCTTC 2 cut(s) 26, 1508
BstC8I GCNNGC 5 cut(s) 79, 774, 889, 1199, 1462
BstDEI CTNAG 3 cut(s) 396, 1023, 1155
BstDSI CCRYGG 1 cut(s) 214
BstEII GGTNACC 1 cut(s) 608
BstF5I GGATG 4 cut(s) 295, 935, 940, 1462
BstFNI CGCG 1 cut(s) 782
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 6 cut(s) 36, 511, 630, 994, 1040, 1264
BstMAI GTCTC 1 cut(s) 915
BstMBI GATC 6 cut(s) 33, 508, 627, 991, 1037, 1261
BstMWI GCNNNNNNNGC 7 cut(s) 95, 98, 101, 104, 107, 179, 788
BstNI CCWGG 1 cut(s) 690
BstPI GGTNACC 1 cut(s) 608
BstSCI CCNGG 1 cut(s) 688
BstSFI CTRYAG 1 cut(s) 738
BstUI CGCG 1 cut(s) 782
BstX2I RGATCY 2 cut(s) 508, 1037
BstXI CCANNNNNNTGG 1 cut(s) 234
BstYI RGATCY 2 cut(s) 508, 1037
Bsu36I CCTNAGG 1 cut(s) 396
BsuI GTATCC 1 cut(s) 1412
BsuRI GGCC 1 cut(s) 759
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 4 cut(s) 295, 935, 940, 1462
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 54, 502
Cac8I GCNNGC 5 cut(s) 79, 774, 889, 1199, 1462
CaiI CAGNNNCTG 1 cut(s) 1187
CfoI GCGC 1 cut(s) 784
Cfr10I RCCGGY 1 cut(s) 725
Cfr13I GGNCC 1 cut(s) 224
CseI GACGC 1 cut(s) 502
CsiI ACCWGGT 1 cut(s) 688
Csp6I GTAC 4 cut(s) 667, 1255, 1344, 1528
CviAII CATG 4 cut(s) 215, 378, 973, 1428
CviQI GTAC 4 cut(s) 667, 1255, 1344, 1528
DdeI CTNAG 3 cut(s) 396, 1023, 1155
DpnI GATC 6 cut(s) 35, 510, 629, 993, 1039, 1263
DpnII GATC 6 cut(s) 33, 508, 627, 991, 1037, 1261
DraI TTTAAA 1 cut(s) 549
Eam1104I CTCTTC 2 cut(s) 26, 1508
EarI CTCTTC 2 cut(s) 26, 1508
Ecl136II GAGCTC 1 cut(s) 463
Eco130I CCWWGG 2 cut(s) 214, 1051
Eco24I GRGCYC 1 cut(s) 465
Eco31I GGTCTC 1 cut(s) 915
Eco32I GATATC 1 cut(s) 1520
Eco47I GGWCC 1 cut(s) 224
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 2 cut(s) 216, 346
Eco81I CCTNAGG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 1265
Eco91I GGTNACC 1 cut(s) 608
EcoICRI GAGCTC 1 cut(s) 463
EcoO65I GGTNACC 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 1221
EcoRII CCWGG 1 cut(s) 688
EcoRV GATATC 1 cut(s) 1520
EcoT14I CCWWGG 2 cut(s) 214, 1051
EcoT22I ATGCAT 1 cut(s) 1140
EcoT38I GRGCYC 1 cut(s) 465
ErhI CCWWGG 2 cut(s) 214, 1051
FaeI CATG 4 cut(s) 218, 381, 976, 1431
FalI AAGNNNNNCTT 2 cut(s) 1493, 1525
FaqI GGGAC 2 cut(s) 237, 251
FatI CATG 4 cut(s) 214, 377, 972, 1427
FauNDI CATATG 1 cut(s) 233
FokI GGATG 4 cut(s) 302, 927, 942, 1469
FriOI GRGCYC 1 cut(s) 465
FspBI CTAG 4 cut(s) 156, 906, 1043, 1104
GlaI GCGC 1 cut(s) 783
GsaI CCCAGC 2 cut(s) 155, 1318
HaeIII GGCC 1 cut(s) 759
HapII CCGG 1 cut(s) 726
HgaI GACGC 1 cut(s) 502
HhaI GCGC 1 cut(s) 784
Hin1II CATG 4 cut(s) 218, 381, 976, 1431
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HincII GTYRAC 2 cut(s) 661, 1114
HindII GTYRAC 2 cut(s) 661, 1114
HinfI GANTC 3 cut(s) 127, 356, 1482
HpaII CCGG 1 cut(s) 726
HphI GGTGA 8 cut(s) 578, 620, 968, 1001, 1307, 1417, 1423, 1477
Hpy166II GTNNAC 3 cut(s) 421, 661, 1114
Hpy188I TCNGA 7 cut(s) 170, 192, 365, 636, 713, 877, 1130
Hpy8I GTNNAC 3 cut(s) 421, 661, 1114
Hpy99I CGWCG 1 cut(s) 1496
HpyAV CCTTC 4 cut(s) 191, 370, 422, 1402
HpyCH4III ACNGT 2 cut(s) 656, 1471
HpyCH4IV ACGT 1 cut(s) 1491
HpyCH4V TGCA 8 cut(s) 686, 720, 791, 887, 1082, 1138, 1460, 1535
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 98, 101, 104, 107, 179, 788
HpyF3I CTNAG 3 cut(s) 396, 1023, 1155
HpySE526I ACGT 1 cut(s) 1491
Hsp92II CATG 4 cut(s) 218, 381, 976, 1431
HspAI GCGC 1 cut(s) 782
Kzo9I GATC 6 cut(s) 33, 508, 627, 991, 1037, 1261
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 5 cut(s) 115, 170, 200, 1032, 1316
LweI GCATC 3 cut(s) 686, 1318, 1447
MabI ACCWGGT 1 cut(s) 688
MaeI CTAG 4 cut(s) 156, 906, 1043, 1104
MaeII ACGT 1 cut(s) 1491
MaeIII GTNAC 7 cut(s) 490, 608, 646, 728, 850, 946, 956
MalI GATC 6 cut(s) 35, 510, 629, 993, 1039, 1263
MboI GATC 6 cut(s) 33, 508, 627, 991, 1037, 1261
MboII GAAGA 9 cut(s) 13, 16, 157, 335, 539, 817, 1028, 1117, 1525
MflI RGATCY 2 cut(s) 508, 1037
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 7 cut(s) 332, 437, 715, 793, 1004, 1060, 1221
MlyI GAGTC 2 cut(s) 350, 1476
MmeI TCCRAC 1 cut(s) 691
Mph1103I ATGCAT 1 cut(s) 1140
MseI TTAA 2 cut(s) 548, 618
MslI CAYNNNNRTG 2 cut(s) 213, 315
MspI CCGG 1 cut(s) 726
MspR9I CCNGG 1 cut(s) 690
MvaI CCWGG 1 cut(s) 690
MvnI CGCG 1 cut(s) 782
MwoI GCNNNNNNNGC 7 cut(s) 95, 98, 101, 104, 107, 179, 788
NcoI CCATGG 1 cut(s) 214
NdeI CATATG 1 cut(s) 233
NdeII GATC 6 cut(s) 33, 508, 627, 991, 1037, 1261
NlaIII CATG 4 cut(s) 218, 381, 976, 1431
NlaIV GGNNCC 3 cut(s) 225, 679, 1170
NmuCI GTSAC 4 cut(s) 490, 608, 850, 956
NsiI ATGCAT 1 cut(s) 1140
PciSI GCTCTTC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 127
PleI GAGTC 2 cut(s) 350, 1476
PpsI GAGTC 2 cut(s) 350, 1476
PsiI TTATAA 1 cut(s) 455
Psp124BI GAGCTC 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 688
PspEI GGTNACC 1 cut(s) 608
PspFI CCCAGC 2 cut(s) 151, 1314
PspGI CCWGG 1 cut(s) 688
PspN4I GGNNCC 3 cut(s) 225, 679, 1170
PspPI GGNCC 1 cut(s) 224
PstNI CAGNNNCTG 1 cut(s) 1187
PsuI RGATCY 2 cut(s) 508, 1037
RsaI GTAC 4 cut(s) 668, 1256, 1345, 1529
RsaNI GTAC 4 cut(s) 667, 1255, 1344, 1528
RseI CAYNNNNRTG 2 cut(s) 213, 315
SacI GAGCTC 1 cut(s) 465
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 548, 618
Sau3AI GATC 6 cut(s) 33, 508, 627, 991, 1037, 1261
Sau96I GGNCC 1 cut(s) 224
ScaI AGTACT 1 cut(s) 1529
SchI GAGTC 2 cut(s) 350, 1476
ScrFI CCNGG 1 cut(s) 690
SduI GDGCHC 1 cut(s) 465
SexAI ACCWGGT 1 cut(s) 688
SfaNI GCATC 3 cut(s) 686, 1318, 1447
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 1 cut(s) 224
SmiMI CAYNNNNRTG 2 cut(s) 213, 315
SmlI CTYRAG 2 cut(s) 1334, 1523
SmoI CTYRAG 2 cut(s) 1334, 1523
SpeI ACTAGT 1 cut(s) 1103
Sse9I AATT 7 cut(s) 332, 437, 715, 793, 1004, 1060, 1221
SsiI CCGC 2 cut(s) 89, 723
SspMI CTAG 4 cut(s) 156, 906, 1043, 1104
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 1 cut(s) 688
StyI CCWWGG 2 cut(s) 214, 1051
TaaI ACNGT 2 cut(s) 656, 1471
TaiI ACGT 1 cut(s) 1494
TaqI TCGA 6 cut(s) 125, 470, 694, 1225, 1474, 1494
TaqII GACCGA 1 cut(s) 627
TasI AATT 7 cut(s) 332, 437, 715, 793, 1004, 1060, 1221
TatI WGTACW 3 cut(s) 1254, 1343, 1527
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 548, 618
Tru9I TTAA 2 cut(s) 548, 618
TscAI CASTG 2 cut(s) 61, 509
TseFI GTSAC 4 cut(s) 490, 608, 850, 956
Tsp45I GTSAC 4 cut(s) 490, 608, 850, 956
TspDTI ATGAA 5 cut(s) 16, 151, 353, 1365, 1464
TspRI CASTG 2 cut(s) 61, 509
VpaK11BI GGWCC 1 cut(s) 224
XapI RAATTY 4 cut(s) 437, 793, 1004, 1221
XbaI TCTAGA 1 cut(s) 1042
XspI CTAG 4 cut(s) 156, 906, 1043, 1104
ZrmI AGTACT 1 cut(s) 1529
Zsp2I ATGCAT 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.