RchiOBHm_Chr1g0318081

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002378
Physical Location & Seq
Forward (+)
5590981 .. 5591641
661 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 432 bp
ATGATGAGGGACGTGTCGAGCTGCAACACGTACGATTACGGAGACGCCCAATACTGGGACGCCCGCTACGTCCAAGAAGGTGACTCCTTATTCGACTGGTGCCAGCGCTACTCTGCTCTCCGCCCTTTCATCCGCAACTTCATTCCCCTCACCTCCTCTCCTGTTCTCGTCGTCGGCTCCGGCAATGCCGTTATGTCGGAGGACATGGCGAAAGATGGATATGAAGACATAATGAACATTGACATCTCATCAGTGGCTATCAACCTCATGAAGACCAGAAACAAGCACATTCCTCAGTTGAAAGACAAGCAAATGGATGTGAGGGATATGAGCTTCTTCCCAGATGAATCCTTTGACGGTATTATTGATAAAGGTACAGAGTCTGTTTATTTGTTGTGGTTGATTTTTAAAATACGCGGTATCGTATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

143

Amino Acids

16.54

Weight (kDa)

4.67

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_25 PF13649 55 - 123 1.8e-07 Methyltransferase domain
Methyltransf_11 PF08241 56 - 122 5.8e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 426
AccII CGCG 1 cut(s) 417
AciI CCGC 4 cut(s) 64, 121, 133, 417
AcyI GRCGYC 2 cut(s) 45, 60
AfaI GTAC 2 cut(s) 32, 376
AfeI AGCGCT 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 54, 55
AflIII ACRYGT 2 cut(s) 12, 27
AgsI TTSAA 1 cut(s) 301
AjiI CACGTC 1 cut(s) 13
AluBI AGCT 2 cut(s) 21, 333
AluI AGCT 2 cut(s) 21, 333
Alw26I GTCTC 1 cut(s) 36
AlwNI CAGNNNCTG 1 cut(s) 383
Aor51HI AGCGCT 1 cut(s) 107
ApeKI GCWGC 1 cut(s) 21
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 1 cut(s) 108
AsuHPI GGTGA 2 cut(s) 92, 142
BanI GGYRCC 1 cut(s) 99
BbsI GAAGAC 2 cut(s) 231, 278
BbvI GCAGC 1 cut(s) 8
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 36
BfoI RGCGCY 1 cut(s) 109
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
BmgBI CACGTC 1 cut(s) 13
BmiI GGNNCC 2 cut(s) 101, 178
BmrI ACTGGG 1 cut(s) 64
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 2 cut(s) 231, 278
BsaAI YACGTR 1 cut(s) 30
BsaHI GRCGYC 2 cut(s) 45, 60
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 55
Bse1I ACTGG 2 cut(s) 59, 101
Bse3DI GCAATG 1 cut(s) 190
BseGI GGATG 2 cut(s) 129, 322
BseLI CCNNNNNNNGG 2 cut(s) 54, 55
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 59, 101
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 1 cut(s) 8
Bsh1236I CGCG 1 cut(s) 417
BshNI GGYRCC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 180
BsiWI CGTACG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 23, 71
BslI CCNNNNNNNGG 2 cut(s) 54, 55
BsmAI GTCTC 1 cut(s) 36
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 2 cut(s) 23, 71
BspACI CCGC 4 cut(s) 64, 121, 133, 417
BspCNI CTCAG 1 cut(s) 307
BspFNI CGCG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 101, 178
BspT107I GGYRCC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 190
BsrI ACTGG 2 cut(s) 59, 101
BssNAI GTATAC 1 cut(s) 427
BssNI GRCGYC 2 cut(s) 45, 60
Bst1107I GTATAC 1 cut(s) 427
Bst4CI ACNGT 1 cut(s) 359
BstACI GRCGYC 2 cut(s) 45, 60
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 64, 104
BstDEI CTNAG 1 cut(s) 294
BstF5I GGATG 2 cut(s) 129, 322
BstFNI CGCG 1 cut(s) 417
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 1 cut(s) 108
BstMAI GTCTC 1 cut(s) 36
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 8
BstV2I GAAGAC 2 cut(s) 231, 278
BstZ17I GTATAC 1 cut(s) 427
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 2 cut(s) 129, 322
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 64, 104
CaiI CAGNNNCTG 1 cut(s) 383
CciI TCATGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 108
CseI GACGC 2 cut(s) 53, 68
Csp6I GTAC 2 cut(s) 31, 375
CviAII CATG 2 cut(s) 205, 268
CviJI RGCY 4 cut(s) 21, 177, 257, 333
CviKI_1 RGCY 4 cut(s) 21, 177, 257, 333
CviQI GTAC 2 cut(s) 31, 375
DdeI CTNAG 1 cut(s) 294
DraI TTTAAA 1 cut(s) 409
EciI GGCGGA 1 cut(s) 110
Eco47III AGCGCT 1 cut(s) 107
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 2 cut(s) 208, 271
FaiI YATR 7 cut(s) 194, 206, 222, 230, 269, 329, 427
FaqI GGGAC 2 cut(s) 23, 71
FatI CATG 2 cut(s) 204, 267
FauI CCCGC 1 cut(s) 71
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 1 cut(s) 22
FokI GGATG 2 cut(s) 116, 329
Fsp4HI GCNGC 1 cut(s) 22
GlaI GCGC 1 cut(s) 107
GluI GCNGC 1 cut(s) 22
HaeII RGCGCY 1 cut(s) 109
HapII CCGG 1 cut(s) 180
HgaI GACGC 2 cut(s) 53, 68
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 2 cut(s) 45, 60
Hin1II CATG 2 cut(s) 208, 271
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HinfI GANTC 3 cut(s) 83, 347, 380
HpaII CCGG 1 cut(s) 180
HphI GGTGA 2 cut(s) 92, 142
Hpy166II GTNNAC 1 cut(s) 427
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 1 cut(s) 268
Hpy8I GTNNAC 1 cut(s) 427
Hpy99I CGWCG 2 cut(s) 173, 176
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4IV ACGT 3 cut(s) 12, 29, 69
HpyCH4V TGCA 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 3 cut(s) 12, 29, 69
Hsp92I GRCGYC 2 cut(s) 45, 60
Hsp92II CATG 2 cut(s) 208, 271
HspAI GCGC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 7 cut(s) 40, 82, 116, 174, 193, 289, 354
Lsp1109I GCAGC 1 cut(s) 8
MaeII ACGT 3 cut(s) 12, 29, 69
MaeIII GTNAC 1 cut(s) 80
MboII GAAGA 3 cut(s) 236, 283, 328
MlyI GAGTC 2 cut(s) 77, 389
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 7 cut(s) 158, 163, 166, 193, 275, 303, 315
MseI TTAA 1 cut(s) 408
MspI CCGG 1 cut(s) 180
MvnI CGCG 1 cut(s) 417
NlaIII CATG 2 cut(s) 208, 271
NlaIV GGNNCC 2 cut(s) 101, 178
NmuCI GTSAC 1 cut(s) 80
PagI TCATGA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PfeI GAWTC 1 cut(s) 347
Pfl23II CGTACG 1 cut(s) 30
PkrI GCNGC 1 cut(s) 23
PleI GAGTC 2 cut(s) 77, 388
PpsI GAGTC 2 cut(s) 77, 388
Ppu21I YACGTR 1 cut(s) 30
PspLI CGTACG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 101, 178
PstNI CAGNNNCTG 1 cut(s) 383
RsaI GTAC 2 cut(s) 32, 376
RsaNI GTAC 2 cut(s) 31, 375
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 1 cut(s) 22
SchI GAGTC 2 cut(s) 77, 389
SetI ASST 9 cut(s) 15, 23, 32, 72, 82, 155, 267, 335, 376
SsiI CCGC 4 cut(s) 64, 121, 133, 417
TaaI ACNGT 1 cut(s) 359
TaiI ACGT 3 cut(s) 15, 32, 72
TaqI TCGA 2 cut(s) 17, 93
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 6 cut(s) 118, 130, 237, 248, 284, 360
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.