RchiOBHm_Chr1g0356681

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004611
Physical Location & Seq
Reverse (-)
49091302 .. 49094399
3098 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58200

Sequence Viewer

Length: 552 bp
ATGTCTTCCCCAAGCAAACGCAGAGAGATGGACTTGATGAAACTGATGATGAGTGATTACAAGGTGGACATGATTAATGATGGAATGCATGAGTTCTATGTAGATTTCCATGGACCCAGCGAGAGTCCTTATCAGGGAGGCGTGTGGAGGATAAGAGTTGAACTACCAGATGCTTATCCCTATAAATCTCCCTCAATAGGCTTTATCAATAAGATCTACCACCCAAATGTTGATGAGATGTCAGGTTCGGTTTGCTTAGATGTTATCAATCAAACATGGAGCCCCATGTTTGATTTGGTAAATGTATTTGAAGTATTCCTGCCACAGCTTCTGCTGTATCCCAATCCATCAGACCCTTTAAATGGAGAGGCTGCAGCTTTAATGATGCGTGACCGAGCTTCTTATGAACAAAGAGTCAAAGAATACTGTCTGAAGTATGCCAAGCCTGAAGATATAGGAGCTGTCCCAGAAGACAAATCAAGTGATGAAGAACTGAGTGAAGATGAATCTGACTCCTGTGATGACCAAGTGGCCGGTCAAGCAGATCCATAG

Protein Analysis

183

Amino Acids

20.84

Weight (kDa)

4.34

Isoelectric Point (pI)

58.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 9 - 142 4.7e-40 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 539
AcoI YGGCCR 1 cut(s) 531
AcuI CTGAAG 2 cut(s) 452, 468
AfiI CCNNNNNNNGG 3 cut(s) 134, 197, 362
AgsI TTSAA 2 cut(s) 161, 311
AluBI AGCT 4 cut(s) 328, 377, 398, 461
AluI AGCT 4 cut(s) 328, 377, 398, 461
AlwI GGATC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 331
AoxI GGCC 1 cut(s) 531
ApeKI GCWGC 2 cut(s) 371, 374
AseI ATTAAT 1 cut(s) 75
AspS9I GGNCC 1 cut(s) 113
AvaII GGWCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 477
BbvI GCAGC 2 cut(s) 358, 386
BccI CCATC 3 cut(s) 22, 74, 355
BciVI GTATCC 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 372
BfuI GTATCC 1 cut(s) 348
BglII AGATCT 1 cut(s) 213
BisI GCNGC 2 cut(s) 372, 375
BlsI GCNGC 2 cut(s) 373, 376
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 113
BmiI GGNNCC 2 cut(s) 115, 281
BmsI GCATC 2 cut(s) 160, 375
BpiI GAAGAC 1 cut(s) 477
BsaBI GATNNNNATC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 109
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 197, 362
Bse118I RCCGGY 1 cut(s) 533
Bse8I GATNNNNATC 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 109
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 3 cut(s) 134, 197, 362
BseMII CTCAG 1 cut(s) 485
BseXI GCAGC 2 cut(s) 358, 386
BseYI CCCAGC 1 cut(s) 116
BshFI GGCC 1 cut(s) 533
BsiSI CCGG 1 cut(s) 534
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 3 cut(s) 134, 197, 362
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 2 cut(s) 213, 544
Bsp19I CCATGG 1 cut(s) 109
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 1 cut(s) 486
BspLI GGNNCC 2 cut(s) 115, 281
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 1 cut(s) 539
BsrFI RCCGGY 1 cut(s) 533
BssAI RCCGGY 1 cut(s) 533
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 2 cut(s) 213, 544
BssT1I CCWWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 428
BstDEI CTNAG 2 cut(s) 256, 494
BstDSI CCRYGG 1 cut(s) 109
BstKTI GATC 2 cut(s) 216, 547
BstMBI GATC 2 cut(s) 213, 544
BstMWI GCNNNNNNNGC 1 cut(s) 539
BstSFI CTRYAG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 358, 386
BstV2I GAAGAC 1 cut(s) 477
BstX2I RGATCY 2 cut(s) 213, 544
BstYI RGATCY 2 cut(s) 213, 544
BsuI GTATCC 1 cut(s) 348
BsuRI GGCC 1 cut(s) 533
BtgI CCRYGG 1 cut(s) 109
CaiI CAGNNNCTG 1 cut(s) 331
Cfr10I RCCGGY 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 113
CviAII CATG 5 cut(s) 70, 89, 110, 276, 286
CviJI RGCY 9 cut(s) 201, 282, 328, 371, 377, 398, 445, 461, 533
CviKI_1 RGCY 9 cut(s) 201, 282, 328, 371, 377, 398, 445, 461, 533
DdeI CTNAG 2 cut(s) 256, 494
DpnI GATC 2 cut(s) 215, 546
DpnII GATC 2 cut(s) 213, 544
DraI TTTAAA 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 113
Eco57I CTGAAG 2 cut(s) 452, 468
EcoT14I CCWWGG 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 109
FaeI CATG 5 cut(s) 73, 92, 113, 279, 289
FaqI GGGAC 1 cut(s) 449
FatI CATG 5 cut(s) 69, 88, 109, 275, 285
Fnu4HI GCNGC 2 cut(s) 372, 375
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 2 cut(s) 372, 375
GluI GCNGC 2 cut(s) 372, 375
GsaI CCCAGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 533
HapII CCGG 1 cut(s) 534
Hin1II CATG 5 cut(s) 73, 92, 113, 279, 289
HinfI GANTC 4 cut(s) 124, 414, 506, 512
HpaII CCGG 1 cut(s) 534
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 352, 432, 511
Hpy8I GTNNAC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4V TGCA 2 cut(s) 88, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 539
HpyF3I CTNAG 2 cut(s) 256, 494
Hsp92II CATG 5 cut(s) 73, 92, 113, 279, 289
Kzo9I GATC 2 cut(s) 213, 544
LmnI GCTCC 2 cut(s) 279, 458
LpnPI CCDG 9 cut(s) 119, 130, 180, 228, 332, 459, 480, 529, 547
Lsp1109I GCAGC 2 cut(s) 358, 386
LweI GCATC 2 cut(s) 160, 375
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 215, 546
MboI GATC 2 cut(s) 213, 544
MboII GAAGA 4 cut(s) 461, 482, 500, 512
MflI RGATCY 2 cut(s) 213, 544
MhlI GDGCHC 1 cut(s) 284
MlyI GAGTC 3 cut(s) 133, 423, 506
MnlI CCTC 4 cut(s) 131, 141, 202, 361
Mph1103I ATGCAT 1 cut(s) 90
MseI TTAA 3 cut(s) 75, 359, 380
MslI CAYNNNNRTG 1 cut(s) 225
MspI CCGG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 90
MwoI GCNNNNNNNGC 1 cut(s) 539
NcoI CCATGG 1 cut(s) 109
NdeII GATC 2 cut(s) 213, 544
NlaIII CATG 5 cut(s) 73, 92, 113, 279, 289
NlaIV GGNNCC 2 cut(s) 115, 281
NmuCI GTSAC 1 cut(s) 389
NsiI ATGCAT 1 cut(s) 90
PctI GAATGC 1 cut(s) 90
PfeI GAWTC 1 cut(s) 506
PkrI GCNGC 2 cut(s) 373, 376
PleI GAGTC 3 cut(s) 132, 422, 506
PpsI GAGTC 3 cut(s) 132, 422, 506
PshBI ATTAAT 1 cut(s) 75
PspFI CCCAGC 1 cut(s) 116
PspN4I GGNNCC 2 cut(s) 115, 281
PspPI GGNCC 1 cut(s) 113
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 2 cut(s) 213, 544
RseI CAYNNNNRTG 1 cut(s) 225
SaqAI TTAA 3 cut(s) 75, 359, 380
SatI GCNGC 2 cut(s) 372, 375
Sau3AI GATC 2 cut(s) 213, 544
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 3 cut(s) 133, 423, 506
SduI GDGCHC 1 cut(s) 284
SetI ASST 6 cut(s) 66, 247, 330, 379, 400, 463
SfaNI GCATC 2 cut(s) 160, 375
SfcI CTRYAG 1 cut(s) 372
SinI GGWCC 1 cut(s) 113
SmiMI CAYNNNNRTG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 428
TaqII GACCGA 1 cut(s) 408
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 3 cut(s) 75, 359, 380
Tru9I TTAA 3 cut(s) 75, 359, 380
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 2 cut(s) 371, 374
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 4 cut(s) 53, 420, 501, 519
VpaK11BI GGWCC 1 cut(s) 113
VspI ATTAAT 1 cut(s) 75
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.