RchiOBHm_Chr2g0097611

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001719
Physical Location & Seq
Forward (+)
10179561 .. 10181048
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47248

Sequence Viewer

Length: 1032 bp
ATGAACTCGCCTCGTTTGCACTCTCATAACAACTCTGAGCTTGAAGAGTTATGGAGTTCAAAGGAGGGTTTAGTTCGGTGCTCGGCAAATCACGTCCCTTTATCTCCAATAGGATTCTTGGAGCGAGCTGCAAAGGCCTACAGGGACAGAACATCTGTAATCTATGGCTCTCTGAAATACACTTGGGCTCAAACCCATCAACGTTGCCTTAAATTAGCTTCCGCTCTTACTCGGTTGGGAATTTCTCGCGGTGATGTGTTGAGTGGTTGCAACTTTGGCACCAATGTCCCAACTATGCATGAGCTTCATTTTGCAGCTCCAATGGCTGGGGCAGTTCTTTGTACACTAAATGCTCGGCTTGATTCAGCTATGATATCTGTTCTTCTTTGTCATTCTGAAGCTAAAATCGTCTTTGTCGATCACCAATTACTTGGAATTGTTGATGGAGCACTTGAATTAATTACCGAAAAAGCTGTTTCAAAACCGCCTATTGTAGTTTTAATTGCTGATTCTGATGGATCATCATCTCCAAACAGCTCTACTTCAGAAACTGAACCACCTTATGAGTATGAAAGTCTTTTAGAAAGTGGAAATAGTGGATTTGAGATTAGGAGACCAAAAAGTGAATGGGATCCTATCAGTGTAAATTACACCTCTGGCACAACATCAAGGCCGAAAGGGGTTGTCTATAATCACCGCGGCGCTTATCTCAATTCACTGGCCACAGTTTTGCTTTGCAGAATAGGCTCAATGCCGGTTAATCTATGGACTGCGCCTATGTTTCATTGCCATGGATGGTCTCTCATTTGGGGTTTGGCTGCTCAGGGTGGTACTAAGTATAGCTCATGGAAACCTGAGTGGGACTCTCTTCCTTCAGATGAGAGATCGAAGCTTAAAGCTCGGCAAGGCGTGCAACATGTTGCTTTGGAAGAGGTTGACATAAAAGATTCTGTCACTATGGAAAGTGTACCGCTTGATGGTAAGACAATGGGTGGAATTATGTTCAGGGGAAACACTGTGATGAGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

343

Amino Acids

37.47

Weight (kDa)

6.64

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 40 - 278 1.4e-40 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AccB7I CCANNNNNTGG 1 cut(s) 326
AccBSI CCGCTC 1 cut(s) 224
AccII CGCG 2 cut(s) 249, 699
AciI CCGC 6 cut(s) 222, 249, 485, 697, 699, 971
AclI AACGTT 1 cut(s) 202
AclWI GGATC 3 cut(s) 526, 626, 639
AcoI YGGCCR 1 cut(s) 720
AcsI RAATTY 1 cut(s) 240
AcuI CTGAAG 3 cut(s) 417, 528, 858
AfaI GTAC 3 cut(s) 343, 832, 969
AfiI CCNNNNNNNGG 2 cut(s) 326, 977
AflIII ACRYGT 1 cut(s) 916
AgsI TTSAA 4 cut(s) 44, 60, 455, 480
AjiI CACGTC 1 cut(s) 94
Alw21I GWGCWC 2 cut(s) 83, 451
Alw26I GTCTC 2 cut(s) 607, 804
AlwI GGATC 3 cut(s) 526, 626, 639
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 3 cut(s) 135, 671, 720
ApeKI GCWGC 3 cut(s) 128, 314, 818
ApoI RAATTY 1 cut(s) 240
AseI ATTAAT 1 cut(s) 458
AspLEI GCGC 2 cut(s) 704, 775
AsuHPI GGTGA 3 cut(s) 263, 413, 686
BalI TGGCCA 1 cut(s) 722
BamHI GGATCC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 190
Bbv12I GWGCWC 2 cut(s) 83, 451
BbvI GCAGC 3 cut(s) 115, 326, 805
BccI CCATC 5 cut(s) 204, 437, 509, 789, 971
BcoDI GTCTC 2 cut(s) 607, 804
BfmI CTRYAG 1 cut(s) 139
BfoI RGCGCY 1 cut(s) 705
BisI GCNGC 4 cut(s) 129, 315, 700, 819
BlsI GCNGC 4 cut(s) 130, 316, 701, 820
BmgBI CACGTC 1 cut(s) 94
BmiI GGNNCC 2 cut(s) 280, 633
BplI GAGNNNNNCTC 2 cut(s) 848, 880
Bpu10I CCTNAGC 1 cut(s) 822
BsaBI GATNNNNATC 1 cut(s) 523
BsaI GGTCTC 2 cut(s) 607, 804
BsaJI CCNNGG 2 cut(s) 697, 790
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 977
Bse118I RCCGGY 1 cut(s) 754
Bse1I ACTGG 1 cut(s) 723
Bse3DI GCAATG 1 cut(s) 784
Bse8I GATNNNNATC 1 cut(s) 523
BseDI CCNNGG 2 cut(s) 697, 790
BseGI GGATG 1 cut(s) 800
BseJI GATNNNNATC 1 cut(s) 523
BseLI CCNNNNNNNGG 2 cut(s) 326, 977
BseMI GCAATG 1 cut(s) 784
BseMII CTCAG 3 cut(s) 27, 836, 846
BseNI ACTGG 1 cut(s) 723
BseXI GCAGC 3 cut(s) 115, 326, 805
BseYI CCCAGC 1 cut(s) 326
Bsh1236I CGCG 2 cut(s) 249, 699
BshFI GGCC 3 cut(s) 137, 673, 722
BshNI GGYRCC 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 83, 451
BsiSI CCGG 1 cut(s) 755
BslFI GGGAC 4 cut(s) 80, 158, 272, 875
BslI CCNNNNNNNGG 2 cut(s) 326, 977
BsmAI GTCTC 2 cut(s) 607, 804
BsmFI GGGAC 4 cut(s) 80, 158, 272, 875
BsnI GGCC 3 cut(s) 137, 673, 722
Bso31I GGTCTC 2 cut(s) 607, 804
Bsp1286I GDGCHC 3 cut(s) 83, 190, 451
Bsp1407I TGTACA 1 cut(s) 341
Bsp143I GATC 4 cut(s) 418, 518, 631, 884
Bsp19I CCATGG 1 cut(s) 790
BspACI CCGC 6 cut(s) 222, 249, 485, 697, 699, 971
BspANI GGCC 3 cut(s) 137, 673, 722
BspCNI CTCAG 3 cut(s) 28, 835, 847
BspFNI CGCG 2 cut(s) 249, 699
BspLI GGNNCC 2 cut(s) 280, 633
BspPI GGATC 3 cut(s) 526, 626, 639
BspT107I GGYRCC 1 cut(s) 278
BspTNI GGTCTC 2 cut(s) 607, 804
BsrBI CCGCTC 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 784
BsrFI RCCGGY 1 cut(s) 754
BsrGI TGTACA 1 cut(s) 341
BsrI ACTGG 1 cut(s) 723
BssAI RCCGGY 1 cut(s) 754
BssECI CCNNGG 2 cut(s) 697, 790
BssMI GATC 4 cut(s) 418, 518, 631, 884
BssT1I CCWWGG 1 cut(s) 790
Bst4CI ACNGT 2 cut(s) 727, 1018
Bst6I CTCTTC 3 cut(s) 39, 873, 924
BstAPI GCANNNNNTGC 1 cut(s) 910
BstAUI TGTACA 1 cut(s) 341
BstC8I GCNNGC 2 cut(s) 126, 911
BstDEI CTNAG 4 cut(s) 36, 822, 834, 855
BstDSI CCRYGG 2 cut(s) 697, 790
BstF5I GGATG 1 cut(s) 800
BstFNI CGCG 2 cut(s) 249, 699
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 2 cut(s) 704, 775
BstKTI GATC 4 cut(s) 421, 521, 634, 887
BstMAI GTCTC 2 cut(s) 607, 804
BstMBI GATC 4 cut(s) 418, 518, 631, 884
BstMWI GCNNNNNNNGC 6 cut(s) 16, 134, 276, 323, 744, 910
BstNSI RCATGY 1 cut(s) 920
BstSFI CTRYAG 1 cut(s) 139
BstUI CGCG 2 cut(s) 249, 699
BstV1I GCAGC 3 cut(s) 115, 326, 805
BstX2I RGATCY 1 cut(s) 631
BstXI CCANNNNNNTGG 1 cut(s) 431
BstYI RGATCY 1 cut(s) 631
BsuRI GGCC 3 cut(s) 137, 673, 722
BtgI CCRYGG 2 cut(s) 697, 790
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 1 cut(s) 800
BtsIMutI CAGTG 3 cut(s) 646, 716, 1014
Cac8I GCNNGC 2 cut(s) 126, 911
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 2 cut(s) 704, 775
Cfr10I RCCGGY 1 cut(s) 754
Cfr42I CCGCGG 1 cut(s) 700
Csp6I GTAC 3 cut(s) 342, 831, 968
CviAII CATG 4 cut(s) 299, 791, 846, 917
CviQI GTAC 3 cut(s) 342, 831, 968
DdeI CTNAG 4 cut(s) 36, 822, 834, 855
DpnI GATC 4 cut(s) 420, 520, 633, 886
DpnII GATC 4 cut(s) 418, 518, 631, 884
EaeI YGGCCR 1 cut(s) 720
Eam1104I CTCTTC 3 cut(s) 39, 873, 924
EarI CTCTTC 3 cut(s) 39, 873, 924
Eco130I CCWWGG 1 cut(s) 790
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 2 cut(s) 607, 804
Eco32I GATATC 1 cut(s) 375
Eco57I CTGAAG 3 cut(s) 417, 528, 858
EcoRV GATATC 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 790
EcoT22I ATGCAT 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 790
FaeI CATG 4 cut(s) 302, 794, 849, 920
FaqI GGGAC 4 cut(s) 80, 158, 272, 875
FatI CATG 4 cut(s) 298, 790, 845, 916
Fnu4HI GCNGC 4 cut(s) 129, 315, 700, 819
FokI GGATG 1 cut(s) 807
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 4 cut(s) 129, 315, 700, 819
GlaI GCGC 2 cut(s) 703, 774
GluI GCNGC 4 cut(s) 129, 315, 700, 819
GsaI CCCAGC 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 3 cut(s) 137, 673, 722
HapII CCGG 1 cut(s) 755
HhaI GCGC 2 cut(s) 704, 775
Hin1II CATG 4 cut(s) 302, 794, 849, 920
Hin6I GCGC 2 cut(s) 702, 773
HinP1I GCGC 2 cut(s) 702, 773
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HindIII AAGCTT 1 cut(s) 890
HinfI GANTC 5 cut(s) 114, 362, 509, 863, 947
HpaII CCGG 1 cut(s) 755
HphI GGTGA 3 cut(s) 263, 413, 686
Hpy166II GTNNAC 3 cut(s) 344, 937, 968
Hpy188I TCNGA 6 cut(s) 37, 174, 397, 514, 547, 877
Hpy8I GTNNAC 3 cut(s) 344, 937, 968
HpyAV CCTTC 1 cut(s) 882
HpyCH4III ACNGT 2 cut(s) 727, 1018
HpyCH4IV ACGT 2 cut(s) 93, 202
HpyCH4V TGCA 7 cut(s) 19, 131, 270, 298, 314, 738, 913
HpyF10VI GCNNNNNNNGC 6 cut(s) 16, 134, 276, 323, 744, 910
HpyF3I CTNAG 4 cut(s) 36, 822, 834, 855
HpySE526I ACGT 2 cut(s) 93, 202
Hsp92II CATG 4 cut(s) 302, 794, 849, 920
HspAI GCGC 2 cut(s) 702, 773
KspI CCGCGG 1 cut(s) 700
Kzo9I GATC 4 cut(s) 418, 518, 631, 884
LmnI GCTCC 3 cut(s) 121, 322, 446
LpnPI CCDG 8 cut(s) 127, 312, 642, 704, 768, 809, 867, 991
Lsp1109I GCAGC 3 cut(s) 115, 326, 805
MaeII ACGT 2 cut(s) 93, 202
MaeIII GTNAC 1 cut(s) 952
MalI GATC 4 cut(s) 420, 520, 633, 886
MbiI CCGCTC 1 cut(s) 224
MboI GATC 4 cut(s) 418, 518, 631, 884
MboII GAAGA 4 cut(s) 56, 374, 860, 941
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 3 cut(s) 83, 190, 451
MlsI TGGCCA 1 cut(s) 722
MluNI TGGCCA 1 cut(s) 722
MlyI GAGTC 1 cut(s) 857
MnlI CCTC 4 cut(s) 21, 58, 664, 925
Mox20I TGGCCA 1 cut(s) 722
Mph1103I ATGCAT 1 cut(s) 300
MscI TGGCCA 1 cut(s) 722
MseI TTAA 6 cut(s) 210, 458, 500, 759, 894, 1030
MslI CAYNNNNRTG 2 cut(s) 789, 1019
Msp20I TGGCCA 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 699
MspI CCGG 1 cut(s) 755
MvnI CGCG 2 cut(s) 249, 699
MwoI GCNNNNNNNGC 6 cut(s) 16, 134, 276, 323, 744, 910
NcoI CCATGG 1 cut(s) 790
NdeII GATC 4 cut(s) 418, 518, 631, 884
NlaIII CATG 4 cut(s) 302, 794, 849, 920
NlaIV GGNNCC 2 cut(s) 280, 633
NmeAIII GCCGAG 3 cut(s) 62, 334, 880
NmuCI GTSAC 1 cut(s) 952
NsiI ATGCAT 1 cut(s) 300
NspI RCATGY 1 cut(s) 920
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 916
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 4 cut(s) 114, 362, 509, 947
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 4 cut(s) 130, 316, 701, 820
PleI GAGTC 1 cut(s) 857
PpsI GAGTC 1 cut(s) 857
PscI ACATGT 1 cut(s) 916
PshBI ATTAAT 1 cut(s) 458
Psp1406I AACGTT 1 cut(s) 202
PspFI CCCAGC 1 cut(s) 326
PspN4I GGNNCC 2 cut(s) 280, 633
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 631
RsaI GTAC 3 cut(s) 343, 832, 969
RsaNI GTAC 3 cut(s) 342, 831, 968
RseI CAYNNNNRTG 2 cut(s) 789, 1019
SacII CCGCGG 1 cut(s) 700
SaqAI TTAA 6 cut(s) 210, 458, 500, 759, 894, 1030
SatI GCNGC 4 cut(s) 129, 315, 700, 819
Sau3AI GATC 4 cut(s) 418, 518, 631, 884
SchI GAGTC 1 cut(s) 857
SduI GDGCHC 3 cut(s) 83, 190, 451
SfcI CTRYAG 1 cut(s) 139
Sfr303I CCGCGG 1 cut(s) 700
SgrBI CCGCGG 1 cut(s) 700
SmiMI CAYNNNNRTG 2 cut(s) 789, 1019
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 6 cut(s) 222, 249, 485, 697, 699, 971
StuI AGGCCT 1 cut(s) 137
StyI CCWWGG 1 cut(s) 790
TaaI ACNGT 2 cut(s) 727, 1018
TaiI ACGT 2 cut(s) 96, 205
TaqI TCGA 2 cut(s) 417, 887
TatI WGTACW 1 cut(s) 341
TauI GCSGC 1 cut(s) 702
TfiI GAWTC 4 cut(s) 114, 362, 509, 947
Tru1I TTAA 6 cut(s) 210, 458, 500, 759, 894, 1030
Tru9I TTAA 6 cut(s) 210, 458, 500, 759, 894, 1030
TscAI CASTG 3 cut(s) 646, 723, 1021
TseFI GTSAC 1 cut(s) 952
TseI GCWGC 3 cut(s) 128, 314, 818
Tsp45I GTSAC 1 cut(s) 952
TspDTI ATGAA 4 cut(s) 17, 296, 585, 773
TspRI CASTG 3 cut(s) 646, 723, 1021
Van91I CCANNNNNTGG 1 cut(s) 326
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 1 cut(s) 240
XceI RCATGY 1 cut(s) 920
XcmI CCANNNNNNNNNTGG 1 cut(s) 624
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.