Rorug02G0067900

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001719
Physical Location & Seq
Forward (+)
5290040 .. 5290983
944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0067900.1

Sequence Viewer

Length: 591 bp
ATGGACTTCTGCTTCTTTGTTCTACACAAAGAGAGAGAGAGGGGTGGAGAAAGGGAATGGGTTCTGCTTCCGCATCTAGGCCGATTGAGGGTTAAGGGACTCATCCAATTCGCAATAGCATCATGTTATCAAAATGAACATGAACTAAGAATCAGCGAGTGTGGAAGAGGAGGGATTTACTTGATGTCCTGCAAATATTGGTGTTACGCCATTTGCAGAGAAGAGTATGCAGGCTTGCGATCGCTGTTGAGGTCCAAAGACCAAAGGGCAAAACCATTTGGGTTCTGCGGACGAAGAGAAAAAGAGAGGAGACGAAAAAACAAAAAAAGGGAGAAGAGAACAGGGGAAGAAGGGAAGAAGGGAAAAAAAGAAGAGATTCAGAGGAAGAAAGAAAAGGGGAGGAAGAACAAAAGAAAGAAAAGAAGTGGCTCTCCCTCTCGGTATTTGATTTTATGTACGGCAAATCATCTATTGGAATTGGATTTATCATACGACTATGTGGTGGAGGCGAGGGCTACACAAAATTTTGAACCTTGGCGTAATGAGGATGAGGACGCGGATGAGGAGAAGCAGAAGAGGAAGACGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

196

Amino Acids

23.42

Weight (kDa)

9.73

Isoelectric Point (pI)

66.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 557
AciI CCGC 3 cut(s) 71, 288, 557
AcsI RAATTY 1 cut(s) 523
AcyI GRCGYC 1 cut(s) 584
AfaI GTAC 1 cut(s) 457
AfiI CCNNNNNNNGG 2 cut(s) 77, 88
AgsI TTSAA 1 cut(s) 530
Alw26I GTCTC 1 cut(s) 304
AoxI GGCC 1 cut(s) 79
ApoI RAATTY 1 cut(s) 523
AsiSI GCGATCGC 1 cut(s) 242
Asp700I GAANNNNTTC 2 cut(s) 60, 375
AspS9I GGNCC 1 cut(s) 252
AvaII GGWCC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 587
BceAI ACGGC 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 252
BmsI GCATC 2 cut(s) 82, 128
BpiI GAAGAC 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 584
BsaJI CCNNGG 1 cut(s) 533
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 88
BseDI CCNNGG 1 cut(s) 533
BseGI GGATG 3 cut(s) 102, 553, 565
BseLI CCNNNNNNNGG 2 cut(s) 77, 88
BseRI GAGGAG 3 cut(s) 183, 322, 578
Bsh1236I CGCG 1 cut(s) 557
Bsh1285I CGRYCG 1 cut(s) 242
BshFI GGCC 1 cut(s) 81
BsiEI CGRYCG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 2 cut(s) 77, 88
BsmAI GTCTC 1 cut(s) 304
BsmBI CGTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 111
BsnI GGCC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 239
BspACI CCGC 3 cut(s) 71, 288, 557
BspANI GGCC 1 cut(s) 81
BspFNI CGCG 1 cut(s) 557
BssECI CCNNGG 1 cut(s) 533
BssMI GATC 1 cut(s) 239
BssNI GRCGYC 1 cut(s) 584
BssT1I CCWWGG 1 cut(s) 533
Bst6I CTCTTC 6 cut(s) 160, 216, 289, 329, 366, 569
BstACI GRCGYC 1 cut(s) 584
BstC8I GCNNGC 2 cut(s) 232, 236
BstDEI CTNAG 1 cut(s) 146
BstF5I GGATG 3 cut(s) 102, 553, 565
BstFNI CGCG 1 cut(s) 557
BstKTI GATC 1 cut(s) 242
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 239
BstMCI CGRYCG 1 cut(s) 242
BstUI CGCG 1 cut(s) 557
BstV2I GAAGAC 1 cut(s) 587
BsuRI GGCC 1 cut(s) 81
BtsCI GGATG 3 cut(s) 102, 553, 565
Cac8I GCNNGC 2 cut(s) 232, 236
Cfr13I GGNCC 1 cut(s) 252
CseI GACGC 1 cut(s) 563
Csp6I GTAC 1 cut(s) 456
CviAII CATG 3 cut(s) 123, 140, 588
CviJI RGCY 4 cut(s) 81, 234, 429, 515
CviKI_1 RGCY 4 cut(s) 81, 234, 429, 515
CviQI GTAC 1 cut(s) 456
DdeI CTNAG 1 cut(s) 146
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
Eam1104I CTCTTC 6 cut(s) 160, 216, 289, 329, 366, 569
EarI CTCTTC 6 cut(s) 160, 216, 289, 329, 366, 569
Eco130I CCWWGG 1 cut(s) 533
Eco47I GGWCC 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 533
ErhI CCWWGG 1 cut(s) 533
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 3 cut(s) 126, 143, 591
FaiI YATR 7 cut(s) 124, 141, 228, 454, 490, 498, 589
FaqI GGGAC 1 cut(s) 111
FatI CATG 3 cut(s) 122, 139, 587
FokI GGATG 3 cut(s) 89, 560, 572
FspBI CTAG 1 cut(s) 77
HaeIII GGCC 1 cut(s) 81
HgaI GACGC 1 cut(s) 563
Hin1I GRCGYC 1 cut(s) 584
Hin1II CATG 3 cut(s) 126, 143, 591
HinfI GANTC 3 cut(s) 99, 150, 376
Hpy188I TCNGA 1 cut(s) 381
HpyAV CCTTC 2 cut(s) 344, 352
HpyCH4V TGCA 3 cut(s) 192, 216, 230
HpyF3I CTNAG 1 cut(s) 146
Hsp92I GRCGYC 1 cut(s) 584
Hsp92II CATG 3 cut(s) 126, 143, 591
Kzo9I GATC 1 cut(s) 239
LpnPI CCDG 3 cut(s) 202, 216, 327
LweI GCATC 2 cut(s) 82, 128
MaeI CTAG 1 cut(s) 77
MaeIII GTNAC 1 cut(s) 203
MalI GATC 1 cut(s) 241
MboI GATC 1 cut(s) 239
MluCI AATT 3 cut(s) 107, 476, 523
MlyI GAGTC 1 cut(s) 93
MroXI GAANNNNTTC 2 cut(s) 60, 375
MseI TTAA 1 cut(s) 93
MvnI CGCG 1 cut(s) 557
NdeII GATC 1 cut(s) 239
NlaIII CATG 3 cut(s) 126, 143, 591
PdmI GAANNNNTTC 2 cut(s) 60, 375
PfeI GAWTC 2 cut(s) 150, 376
Ple19I CGATCG 1 cut(s) 242
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 252
PvuI CGATCG 1 cut(s) 242
RgaI GCGATCGC 1 cut(s) 242
RsaI GTAC 1 cut(s) 457
RsaNI GTAC 1 cut(s) 456
SaqAI TTAA 1 cut(s) 93
Sau3AI GATC 1 cut(s) 239
Sau96I GGNCC 1 cut(s) 252
SchI GAGTC 1 cut(s) 93
SetI ASST 2 cut(s) 254, 535
SfaAI GCGATCGC 1 cut(s) 242
SfaNI GCATC 2 cut(s) 82, 128
SgfI GCGATCGC 1 cut(s) 242
SinI GGWCC 1 cut(s) 252
Sse9I AATT 3 cut(s) 107, 476, 523
SsiI CCGC 3 cut(s) 71, 288, 557
SspI AATATT 1 cut(s) 197
SspMI CTAG 1 cut(s) 77
StyI CCWWGG 1 cut(s) 533
TasI AATT 3 cut(s) 107, 476, 523
TfiI GAWTC 2 cut(s) 150, 376
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TspDTI ATGAA 2 cut(s) 150, 156
VpaK11BI GGWCC 1 cut(s) 252
XapI RAATTY 1 cut(s) 523
XmnI GAANNNNTTC 2 cut(s) 60, 375
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.