Rorug02G0067800

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001719
Physical Location & Seq
Reverse (-)
5268155 .. 5270478
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0067800.1

Sequence Viewer

Length: 1005 bp
ATGACTATCCTCATTGAGCAACCAGATCTTAATGTGCCGCAATTGGAGGTCAAGAAGGTTTTGGACTCTGAGAACAATGAAGAAATGTGTGAATTGGTATTGGATGGTGGGTTTGTGATGCCAAAAGACATAGCCGACGACGATGGATGTGTGAAGCCAGAAGTTCTTGAGTCAAATGATGAGTTCATTGCCCCCGACATCAATGCATTTGGCCAGACATTCAGGGATTATAATGCAGAAAGTGAGAGGCAGAAAAGTGTGGAGGAATTCTATAGGTTGAACCACATTCATCAGACATATGATTTTGTGAAGAGGATGAGGGAGGAGTATAGGAAGATGGATAGAGTGGAAATGAGCATTTGGGAATGCTGTGAGCTCCTTAATGAGGTTGTGGATGAAAGTGATCCTGACTTGGATGAACCGCAAATCGAGCATCTGCTGCAGACAGCTGAAGCAATTAGGAAAGACTATCCTAATGAAGATTGGCTGCATTTGACTGCTCTTATTCATGATCTTGGAAAAGTGCTTCTTCTTCCGAGCTTTGGAGGGCTTCCTCAATGGGCTGTTGTTGGAGACACACATCCTCTTGGTTGTGCTTTTGATGAATCCATTGTTCACCACAAGTATTTCGAGGGCAATCCAGATCATCACAACCCTTCCTATAACACTAAGCACGGAATTTACTCTGAAGGCTGTGGACTAGAAAATGTCATGATTTCTTGGGGACACGATGACTACATGTACTTGGTGGCCAAGGAAAATGGAACAACTTTGCCTGAAGCTGGATTGTTTGTTATCCGTTATCACTCCTTTTATCCTCTTCACAAGTCAGGGGCATATAAACATTTAATGAACAAGGAGGATAAAGAGAACTTGAAGTGGCTTAACATATTCAACAAATATGATCTCTACAGCAAGAGCAAGGTTCGAGTTGATGTCGAAAAAGTCAAGCCCTACTATTTATCTCTTATTGACAAGTATTTCCCAGCAAAGCTGAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

334

Amino Acids

38.98

Weight (kDa)

4.9

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIOX PF05153 85 - 334 2.4e-126 Myo-inositol oxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
AciI CCGC 2 cut(s) 38, 422
AclWI GGATC 1 cut(s) 398
AcoI YGGCCR 2 cut(s) 211, 750
AcsI RAATTY 2 cut(s) 266, 678
AcuI CTGAAG 3 cut(s) 471, 708, 798
AfaI GTAC 1 cut(s) 743
AfiI CCNNNNNNNGG 3 cut(s) 385, 542, 782
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 3 cut(s) 280, 877, 895
AluBI AGCT 5 cut(s) 376, 449, 540, 782, 994
AluI AGCT 5 cut(s) 376, 449, 540, 782, 994
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 1 cut(s) 398
AoxI GGCC 2 cut(s) 211, 750
ApeKI GCWGC 2 cut(s) 439, 487
ApoI RAATTY 2 cut(s) 266, 678
AsuHPI GGTGA 1 cut(s) 608
BalI TGGCCA 2 cut(s) 213, 752
BanII GRGCYC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 2 cut(s) 426, 474
BccI CCATC 4 cut(s) 98, 137, 331, 993
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 1 cut(s) 701
BfmI CTRYAG 3 cut(s) 271, 440, 910
BglII AGATCT 1 cut(s) 25
BisI GCNGC 3 cut(s) 38, 440, 488
BlsI GCNGC 3 cut(s) 39, 441, 489
BmsI GCATC 2 cut(s) 108, 442
BpuEI CTTGAG 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 753
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 542, 782
Bse3DI GCAATG 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 753
BseGI GGATG 6 cut(s) 109, 152, 321, 400, 421, 580
BseLI CCNNNNNNNGG 3 cut(s) 385, 542, 782
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 60, 986
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 2 cut(s) 426, 474
BseYI CCCAGC 1 cut(s) 985
BshFI GGCC 2 cut(s) 213, 752
BsiHKAI GWGCWC 1 cut(s) 378
BslFI GGGAC 1 cut(s) 738
BslI CCNNNNNNNGG 3 cut(s) 385, 542, 782
BsmAI GTCTC 1 cut(s) 567
BsmFI GGGAC 1 cut(s) 738
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 2 cut(s) 213, 752
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 5 cut(s) 25, 403, 511, 643, 904
BspACI CCGC 2 cut(s) 38, 422
BspANI GGCC 2 cut(s) 213, 752
BspCNI CTCAG 2 cut(s) 61, 987
BspHI TCATGA 2 cut(s) 508, 711
BspMAI CTGCAG 1 cut(s) 444
BspPI GGATC 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 186
BssECI CCNNGG 1 cut(s) 753
BssMI GATC 5 cut(s) 25, 403, 511, 643, 904
BssT1I CCWWGG 1 cut(s) 753
Bst6I CTCTTC 2 cut(s) 305, 825
BstAPI GCANNNNNTGC 1 cut(s) 439
BstDEI CTNAG 3 cut(s) 69, 669, 995
BstENI CCTNNNNNAGG 1 cut(s) 383
BstF5I GGATG 6 cut(s) 109, 152, 321, 400, 421, 580
BstKTI GATC 5 cut(s) 28, 406, 514, 646, 907
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 5 cut(s) 25, 403, 511, 643, 904
BstMWI GCNNNNNNNGC 2 cut(s) 430, 439
BstNSI RCATGY 1 cut(s) 742
BstSFI CTRYAG 3 cut(s) 271, 440, 910
BstV1I GCAGC 2 cut(s) 426, 474
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BsuRI GGCC 2 cut(s) 213, 752
BtsCI GGATG 6 cut(s) 109, 152, 321, 400, 421, 580
CciI TCATGA 2 cut(s) 508, 711
Csp6I GTAC 1 cut(s) 742
CviAII CATG 3 cut(s) 509, 712, 739
CviQI GTAC 1 cut(s) 742
DdeI CTNAG 3 cut(s) 69, 669, 995
DpnI GATC 5 cut(s) 27, 405, 513, 645, 906
DpnII GATC 5 cut(s) 25, 403, 511, 643, 904
EaeI YGGCCR 2 cut(s) 211, 750
Eam1104I CTCTTC 2 cut(s) 305, 825
EarI CTCTTC 2 cut(s) 305, 825
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 753
Eco24I GRGCYC 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 3 cut(s) 471, 708, 798
EcoICRI GAGCTC 1 cut(s) 376
EcoNI CCTNNNNNAGG 1 cut(s) 383
EcoRI GAATTC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 753
EcoT22I ATGCAT 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 753
FaeI CATG 3 cut(s) 512, 715, 742
FalI AAGNNNNNCTT 2 cut(s) 513, 545
FaqI GGGAC 1 cut(s) 738
FatI CATG 3 cut(s) 508, 711, 738
FauNDI CATATG 1 cut(s) 298
Fnu4HI GCNGC 3 cut(s) 38, 440, 488
FokI GGATG 6 cut(s) 116, 159, 328, 407, 428, 567
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 3 cut(s) 38, 440, 488
FspBI CTAG 1 cut(s) 701
GluI GCNGC 3 cut(s) 38, 440, 488
GsaI CCCAGC 1 cut(s) 989
HaeIII GGCC 2 cut(s) 213, 752
Hin1II CATG 3 cut(s) 512, 715, 742
HinfI GANTC 3 cut(s) 65, 170, 605
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 2 cut(s) 616, 698
Hpy188I TCNGA 4 cut(s) 70, 294, 537, 688
Hpy188III TCNNGA 6 cut(s) 52, 167, 407, 509, 641, 712
Hpy8I GTNNAC 2 cut(s) 616, 698
Hpy99I CGWCG 2 cut(s) 140, 143
HpyAV CCTTC 3 cut(s) 49, 666, 683
HpyCH4V TGCA 4 cut(s) 206, 236, 442, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 430, 439
HpyF3I CTNAG 3 cut(s) 69, 669, 995
Hsp92II CATG 3 cut(s) 512, 715, 742
Kzo9I GATC 5 cut(s) 25, 403, 511, 643, 904
LmnI GCTCC 1 cut(s) 381
Lsp1109I GCAGC 2 cut(s) 426, 474
LweI GCATC 2 cut(s) 108, 442
MaeI CTAG 1 cut(s) 701
MalI GATC 5 cut(s) 27, 405, 513, 645, 906
MboI GATC 5 cut(s) 25, 403, 511, 643, 904
MboII GAAGA 7 cut(s) 92, 322, 346, 491, 521, 524, 812
MfeI CAATTG 1 cut(s) 41
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 1 cut(s) 378
MlsI TGGCCA 2 cut(s) 213, 752
MluCI AATT 5 cut(s) 41, 92, 266, 456, 678
MluNI TGGCCA 2 cut(s) 213, 752
MlyI GAGTC 2 cut(s) 59, 179
MmeI TCCRAC 1 cut(s) 550
Mox20I TGGCCA 2 cut(s) 213, 752
Mph1103I ATGCAT 1 cut(s) 208
MscI TGGCCA 2 cut(s) 213, 752
MseI TTAA 4 cut(s) 30, 381, 848, 885
Msp20I TGGCCA 2 cut(s) 213, 752
MspA1I CMGCKG 1 cut(s) 449
MunI CAATTG 1 cut(s) 41
Mva1269I GAATGC 1 cut(s) 371
MwoI GCNNNNNNNGC 2 cut(s) 430, 439
NdeI CATATG 1 cut(s) 298
NdeII GATC 5 cut(s) 25, 403, 511, 643, 904
NlaIII CATG 3 cut(s) 512, 715, 742
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 1 cut(s) 742
PagI TCATGA 2 cut(s) 508, 711
PciI ACATGT 1 cut(s) 738
PctI GAATGC 1 cut(s) 371
PfeI GAWTC 1 cut(s) 605
PkrI GCNGC 3 cut(s) 39, 441, 489
PleI GAGTC 2 cut(s) 59, 178
PpsI GAGTC 2 cut(s) 59, 178
PscI ACATGT 1 cut(s) 738
PsiI TTATAA 1 cut(s) 231
Psp124BI GAGCTC 1 cut(s) 378
PspFI CCCAGC 1 cut(s) 985
PstI CTGCAG 1 cut(s) 444
PsuI RGATCY 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 1 cut(s) 743
RsaNI GTAC 1 cut(s) 742
SacI GAGCTC 1 cut(s) 378
SaqAI TTAA 4 cut(s) 30, 381, 848, 885
SatI GCNGC 3 cut(s) 38, 440, 488
Sau3AI GATC 5 cut(s) 25, 403, 511, 643, 904
SchI GAGTC 2 cut(s) 59, 179
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 2 cut(s) 108, 442
SfcI CTRYAG 3 cut(s) 271, 440, 910
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 5 cut(s) 41, 92, 266, 456, 678
SsiI CCGC 2 cut(s) 38, 422
SspMI CTAG 1 cut(s) 701
SstI GAGCTC 1 cut(s) 378
StyI CCWWGG 1 cut(s) 753
TaqI TCGA 4 cut(s) 429, 630, 928, 939
TasI AATT 5 cut(s) 41, 92, 266, 456, 678
TatI WGTACW 1 cut(s) 741
TauI GCSGC 1 cut(s) 40
TfiI GAWTC 1 cut(s) 605
Tru1I TTAA 4 cut(s) 30, 381, 848, 885
Tru9I TTAA 4 cut(s) 30, 381, 848, 885
TseI GCWGC 2 cut(s) 439, 487
TspDTI ATGAA 9 cut(s) 93, 175, 278, 411, 432, 492, 497, 618, 866
TspGWI ACGGA 2 cut(s) 690, 788
XagI CCTNNNNNAGG 1 cut(s) 383
XapI RAATTY 2 cut(s) 266, 678
XceI RCATGY 1 cut(s) 742
XspI CTAG 1 cut(s) 701
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.