RchiOBHm_Chr3g0449271

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001719
Physical Location & Seq
Forward (+)
1128547 .. 1128912
366 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41663

Sequence Viewer

Length: 366 bp
ATGCCTGTTTATCTATGGACTGTGCCTATGTTTCATTGCAATGGACGGTGCCTCATTTGGGGTTTGGCTGCTCAGGGTGGTACTAACATTTGCCTAAGAAAAGTCACTCCAAAAGGCATATATGACAACATTGCTCTACACAATGTAACACACATGGGAGGGTCACCAACTGTGCTGAACATGATTGTTAATTCACCAGAGAGTGACCGAAGACCACTTCCTCACAAGGTGGAAGTAATAACAGGTGGCTCCCCACCCCCGCCACAGATCCTGTTCAATATGGAAGAGATGGGTTTTAGGGTGATTCACTTGTATGGTCTCACAGAGACTTATGGTCCAGGGACCTACATGCTCATGGAAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

121

Amino Acids

13.44

Weight (kDa)

6.95

Isoelectric Point (pI)

63.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 3 - 116 1.4e-22 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 48
AciI CCGC 1 cut(s) 260
AclWI GGATC 1 cut(s) 262
AdeI CACNNNGTG 1 cut(s) 229
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 1 cut(s) 277
AhdI GACNNNNNGTC 1 cut(s) 333
AjnI CCWGG 1 cut(s) 337
Alw26I GTCTC 2 cut(s) 320, 323
AlwI GGATC 1 cut(s) 262
AlwNI CAGNNNCTG 1 cut(s) 271
ApeKI GCWGC 1 cut(s) 68
AspS9I GGNCC 2 cut(s) 335, 342
AsuHPI GGTGA 3 cut(s) 156, 186, 313
AvaII GGWCC 2 cut(s) 335, 342
BanI GGYRCC 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 55
BccI CCATC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 339
BcoDI GTCTC 2 cut(s) 320, 323
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 2 cut(s) 335, 342
BmeRI GACNNNNNGTC 1 cut(s) 333
BmgT120I GGNCC 2 cut(s) 335, 342
BmiI GGNNCC 3 cut(s) 50, 250, 343
BmrFI CCNGG 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 217
Bpu10I CCTNAGC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 1 cut(s) 338
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse3DI GCAATG 3 cut(s) 34, 46, 129
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 338
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 3 cut(s) 34, 46, 129
BseMII CTCAG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 48
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 2 cut(s) 320, 323
BsmFI GGGAC 1 cut(s) 355
Bso31I GGTCTC 1 cut(s) 323
Bsp143I GATC 1 cut(s) 267
BspACI CCGC 1 cut(s) 260
BspCNI CTCAG 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 50, 250, 343
BspPI GGATC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 48
BspTNI GGTCTC 1 cut(s) 323
BsrDI GCAATG 3 cut(s) 34, 46, 129
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 22, 48, 172
Bst6I CTCTTC 1 cut(s) 279
BstDEI CTNAG 2 cut(s) 72, 95
BstEII GGTNACC 1 cut(s) 162
BstKTI GATC 1 cut(s) 270
BstMAI GTCTC 2 cut(s) 320, 323
BstMBI GATC 1 cut(s) 267
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 1 cut(s) 352
BstPI GGTNACC 1 cut(s) 162
BstSCI CCNGG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
CaiI CAGNNNCTG 1 cut(s) 271
Cfr13I GGNCC 2 cut(s) 335, 342
Csp6I GTAC 1 cut(s) 81
CviAII CATG 4 cut(s) 154, 181, 349, 355
CviJI RGCY 2 cut(s) 68, 249
CviKI_1 RGCY 2 cut(s) 68, 249
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 2 cut(s) 72, 95
DpnI GATC 1 cut(s) 269
DpnII GATC 1 cut(s) 267
DraIII CACNNNGTG 1 cut(s) 229
DriI GACNNNNNGTC 1 cut(s) 333
Eam1104I CTCTTC 1 cut(s) 279
Eam1105I GACNNNNNGTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 2 cut(s) 335, 342
Eco91I GGTNACC 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 342
EcoO65I GGTNACC 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 337
FaeI CATG 4 cut(s) 157, 184, 352, 358
FaqI GGGAC 1 cut(s) 355
FatI CATG 4 cut(s) 153, 180, 348, 354
FauI CCCGC 1 cut(s) 267
Fnu4HI GCNGC 1 cut(s) 69
Fsp4HI GCNGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 69
Hin1II CATG 4 cut(s) 157, 184, 352, 358
HinfI GANTC 1 cut(s) 304
HphI GGTGA 3 cut(s) 156, 186, 313
HpyCH4III ACNGT 3 cut(s) 22, 48, 172
HpyCH4V TGCA 1 cut(s) 39
HpyF3I CTNAG 2 cut(s) 72, 95
Hsp92II CATG 4 cut(s) 157, 184, 352, 358
Kzo9I GATC 1 cut(s) 267
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 7 cut(s) 18, 59, 210, 228, 284, 324, 351
Lsp1109I GCAGC 1 cut(s) 55
MaeIII GTNAC 4 cut(s) 103, 145, 162, 203
MalI GATC 1 cut(s) 269
MboI GATC 1 cut(s) 267
MboII GAAGA 2 cut(s) 222, 296
MflI RGATCY 1 cut(s) 267
MluCI AATT 1 cut(s) 190
MnlI CCTC 3 cut(s) 62, 152, 231
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 3 cut(s) 39, 312, 353
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
NdeII GATC 1 cut(s) 267
NlaIII CATG 4 cut(s) 157, 184, 352, 358
NlaIV GGNNCC 3 cut(s) 50, 250, 343
NmuCI GTSAC 3 cut(s) 103, 162, 203
NspI RCATGY 1 cut(s) 352
PfeI GAWTC 1 cut(s) 304
PkrI GCNGC 1 cut(s) 70
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 337
PspEI GGTNACC 1 cut(s) 162
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 3 cut(s) 50, 250, 343
PspPI GGNCC 2 cut(s) 335, 342
PspPPI RGGWCCY 1 cut(s) 342
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 1 cut(s) 267
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
RseI CAYNNNNRTG 3 cut(s) 39, 312, 353
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 1 cut(s) 267
Sau96I GGNCC 2 cut(s) 335, 342
ScrFI CCNGG 1 cut(s) 339
SetI ASST 4 cut(s) 231, 247, 347, 365
SinI GGWCC 2 cut(s) 335, 342
SmiMI CAYNNNNRTG 3 cut(s) 39, 312, 353
Sse9I AATT 1 cut(s) 190
SsiI CCGC 1 cut(s) 260
StyD4I CCNGG 1 cut(s) 337
TaaI ACNGT 3 cut(s) 22, 48, 172
TaqII GACCGA 1 cut(s) 222
TasI AATT 1 cut(s) 190
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseFI GTSAC 3 cut(s) 103, 162, 203
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 3 cut(s) 103, 162, 203
TspDTI ATGAA 1 cut(s) 23
VpaK11BI GGWCC 2 cut(s) 335, 342
XceI RCATGY 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.