Rh2AG114200

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001719
Physical Location & Seq
Forward (+)
10190873 .. 10191280
408 bp
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UTR
Exon/CDS
Intron
Rh2AG114200.1

Sequence Viewer

Length: 408 bp
ATGGATGGTCTCTCATTTGGGGTTTGGCTGCTCAGGGTGGTACTGAGTACTAACATTTGCCTCAGAGAAGTCACTCCAAAAGGCATATTCGACAACATTGCTCTATTCAATGTGACACACATGGGAGGGGCACCAACTGTGCTGAACATGATTGTTAATTCACTAGAGAGTGACCGAAGGCCACTTCCTCACAAGGTGGAAGTAATGACAGGTGGCTCCCCACCACCACCACAGATCCTGTTCAATATGGAAGAGATGGGTTTTGGGATGATTCACTTGTATACAAAGACTTATGGTCCAGGTATTGCTCATGGAAACCTGAGTGGGACTCTCTTCCTTCAGATGAGAGATCAAAGCTTAAAGCTCAGCAAGGCGTGCAACATGTTGCTTTGGAAGAGGTTGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

135

Amino Acids

14.99

Weight (kDa)

9.1

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 12 - 95 5.1e-08 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 130
AccI GTMKAC 1 cut(s) 281
AclWI GGATC 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 323
AdeI CACNNNGTG 1 cut(s) 196
AfaI GTAC 2 cut(s) 42, 49
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 2 cut(s) 109, 244
AhdI GACNNNNNGTC 1 cut(s) 294
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 2 cut(s) 357, 364
AluI AGCT 2 cut(s) 357, 364
Alw26I GTCTC 1 cut(s) 14
AlwI GGATC 1 cut(s) 229
AlwNI CAGNNNCTG 1 cut(s) 238
AoxI GGCC 1 cut(s) 179
ApeKI GCWGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 296
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 130
BbvI GCAGC 1 cut(s) 15
BccI CCATC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 14
BfaI CTAG 1 cut(s) 164
BisI GCNGC 1 cut(s) 29
BlpI GCTNAGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 30
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 296
BmeRI GACNNNNNGTC 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 132, 217
BmrFI CCNGG 1 cut(s) 300
BplI GAGNNNNNCTC 2 cut(s) 313, 345
Bpu10I CCTNAGC 1 cut(s) 32
Bpu1102I GCTNAGC 1 cut(s) 365
BsaI GGTCTC 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 300
BseGI GGATG 2 cut(s) 10, 273
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 5 cut(s) 35, 46, 76, 311, 379
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 1 cut(s) 15
BshFI GGCC 1 cut(s) 181
BshNI GGYRCC 1 cut(s) 130
BslFI GGGAC 1 cut(s) 340
BsmAI GTCTC 1 cut(s) 14
BsmFI GGGAC 1 cut(s) 340
BsnI GGCC 1 cut(s) 181
Bso31I GGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 2 cut(s) 234, 349
Bsp1720I GCTNAGC 1 cut(s) 365
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 5 cut(s) 36, 45, 75, 312, 378
BspLI GGNNCC 2 cut(s) 132, 217
BspPI GGATC 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 130
BspTNI GGTCTC 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 96
BssMI GATC 2 cut(s) 234, 349
BssNAI GTATAC 1 cut(s) 282
Bst1107I GTATAC 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 3 cut(s) 246, 338, 389
BstAPI GCANNNNNTGC 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 5 cut(s) 32, 44, 62, 320, 365
BstF5I GGATG 2 cut(s) 10, 273
BstKTI GATC 2 cut(s) 237, 352
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 2 cut(s) 234, 349
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 298
BstSLI GKGCMC 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
BstZ17I GTATAC 1 cut(s) 282
BsuRI GGCC 1 cut(s) 181
BtsCI GGATG 2 cut(s) 10, 273
Cac8I GCNNGC 1 cut(s) 376
CaiI CAGNNNCTG 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 41, 48
CviAII CATG 4 cut(s) 121, 148, 311, 382
CviJI RGCY 5 cut(s) 28, 181, 216, 357, 364
CviKI_1 RGCY 5 cut(s) 28, 181, 216, 357, 364
CviQI GTAC 2 cut(s) 41, 48
DdeI CTNAG 5 cut(s) 32, 44, 62, 320, 365
DpnI GATC 2 cut(s) 236, 351
DpnII GATC 2 cut(s) 234, 349
DraIII CACNNNGTG 1 cut(s) 196
DriI GACNNNNNGTC 1 cut(s) 294
Eam1104I CTCTTC 3 cut(s) 246, 338, 389
Eam1105I GACNNNNNGTC 1 cut(s) 294
EarI CTCTTC 3 cut(s) 246, 338, 389
Eco31I GGTCTC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 298
FaeI CATG 4 cut(s) 124, 151, 314, 385
FaiI YATR 9 cut(s) 86, 122, 149, 248, 282, 294, 312, 383, 406
FaqI GGGAC 1 cut(s) 340
FatI CATG 4 cut(s) 120, 147, 310, 381
FblI GTMKAC 1 cut(s) 281
Fnu4HI GCNGC 1 cut(s) 29
FokI GGATG 2 cut(s) 17, 280
Fsp4HI GCNGC 1 cut(s) 29
FspBI CTAG 1 cut(s) 164
GluI GCNGC 1 cut(s) 29
HaeIII GGCC 1 cut(s) 181
Hin1II CATG 4 cut(s) 124, 151, 314, 385
HincII GTYRAC 1 cut(s) 402
HindII GTYRAC 1 cut(s) 402
HindIII AAGCTT 1 cut(s) 355
HinfI GANTC 2 cut(s) 271, 328
Hpy166II GTNNAC 2 cut(s) 282, 402
Hpy188I TCNGA 2 cut(s) 65, 342
Hpy8I GTNNAC 2 cut(s) 282, 402
HpyAV CCTTC 2 cut(s) 171, 347
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 378
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpyF3I CTNAG 5 cut(s) 32, 44, 62, 320, 365
Hsp92II CATG 4 cut(s) 124, 151, 314, 385
Kzo9I GATC 2 cut(s) 234, 349
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 6 cut(s) 19, 195, 251, 285, 312, 332
Lsp1109I GCAGC 1 cut(s) 15
MaeI CTAG 1 cut(s) 164
MaeIII GTNAC 3 cut(s) 70, 112, 170
MalI GATC 2 cut(s) 236, 351
MboI GATC 2 cut(s) 234, 349
MboII GAAGA 3 cut(s) 263, 325, 406
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 133
MluCI AATT 1 cut(s) 157
MlyI GAGTC 1 cut(s) 322
MnlI CCTC 4 cut(s) 71, 119, 198, 390
MseI TTAA 2 cut(s) 156, 359
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 375
NdeII GATC 2 cut(s) 234, 349
NlaIII CATG 4 cut(s) 124, 151, 314, 385
NlaIV GGNNCC 2 cut(s) 132, 217
NmuCI GTSAC 3 cut(s) 70, 112, 170
NspI RCATGY 1 cut(s) 385
PciI ACATGT 1 cut(s) 381
PfeI GAWTC 1 cut(s) 271
PkrI GCNGC 1 cut(s) 30
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PscI ACATGT 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 2 cut(s) 132, 217
PspPI GGNCC 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 234
RsaI GTAC 2 cut(s) 42, 49
RsaNI GTAC 2 cut(s) 41, 48
SaqAI TTAA 2 cut(s) 156, 359
SatI GCNGC 1 cut(s) 29
Sau3AI GATC 2 cut(s) 234, 349
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 1 cut(s) 133
SetI ASST 7 cut(s) 198, 214, 304, 321, 359, 366, 401
SinI GGWCC 1 cut(s) 296
Sse9I AATT 1 cut(s) 157
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 90
TaqII GACCGA 1 cut(s) 189
TasI AATT 1 cut(s) 157
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 2 cut(s) 156, 359
Tru9I TTAA 2 cut(s) 156, 359
TseFI GTSAC 3 cut(s) 70, 112, 170
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 3 cut(s) 70, 112, 170
VpaK11BI GGWCC 1 cut(s) 296
XceI RCATGY 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 281
XspI CTAG 1 cut(s) 164
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.