Rorug02G0619100

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001719
Physical Location & Seq
Forward (+)
73643593 .. 73646156
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0619100.1

Sequence Viewer

Length: 822 bp
ATGGCTTGTGCGGCTTGGCTCCAGCCAATTTCCTCACCCCACTCTATCTTTCCATCTCTCTGCCGCCGCCCAATTTTTCCCGCCATCTTCCACCGCTTCACCACCTTCTCCTCCGCCGGCCATCCACTATCCGCCGCCAACCGCCTCACTCCGGTCCACGCGCAGGCCAAACGAGGCCCCTCACTCAAAGAAGACGAAGTAGCTTCTGCTGCGGATGTTGAATTTGAGACGCCGCTGAAAATTGTGGAGTATCCGGACCCTATACTGAGAGCAAAGAACAAGAGAATTGAATCGTTTGATGAAAATTTGAAGAAATTAGTGGAGGAAATGTTTGATATAATGTACAAAACTGATGGCATTGGGCTCTCGGCGCCGCAAGTTGGAATCAATGTGCAGCTGATGGTGTTCAATCCGGTTGGGGAGCGCGGCGAAGGGGAGGAGATTGTGCTTGTGAATCCAAGAGTTAGCAGATATTCGCAGAAAACGAGGCTCTTTGAAGAGGGTTGCTTGTCCTTCCCGGGGATTTATGCTGATGTTCAGAGACCAGAAACTGTGAAAATAGACGCACGGGACATCAGTGGTGCGAGGTTTACAGTCAACTTGTCAGGTCTTCCTGCACGGGTGTTCCAGCATGAATTTGACCATTTGCAGGGTATTCTTTTCTTTGATAGAATGACTGAAGGAGTTCTTGAAAGCATCTCTGAACAGCTACAGGCATTAGAGAAGAAGTATGAAGATAAAACAGGACTTCCAAGTCCTGAAAGGATAGAAAGCCGTAAAAGGATGAAGTTGAAGGCTGCTGCTGGTTTTGGGAAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

273

Amino Acids

30.58

Weight (kDa)

7.03

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pep_deformylase PF01327 79 - 227 2.5e-52 Polypeptide deformylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 753
AccB1I GGYRCC 1 cut(s) 370
AccII CGCG 2 cut(s) 161, 426
AccIII TCCGGA 1 cut(s) 253
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 3 cut(s) 221, 304, 635
AcuI CTGAAG 1 cut(s) 699
AcyI GRCGYC 2 cut(s) 230, 371
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 5 cut(s) 151, 163, 380, 519, 649
AgsI TTSAA 7 cut(s) 221, 290, 310, 409, 497, 692, 793
AluBI AGCT 3 cut(s) 203, 397, 709
AluI AGCT 3 cut(s) 203, 397, 709
Alw26I GTCTC 2 cut(s) 221, 535
AlwNI CAGNNNCTG 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 517
Aor13HI TCCGGA 1 cut(s) 253
AoxI GGCC 3 cut(s) 118, 165, 175
ApeKI GCWGC 4 cut(s) 209, 394, 797, 800
ApoI RAATTY 3 cut(s) 221, 304, 635
Asp700I GAANNNNTTC 1 cut(s) 684
AspLEI GCGC 3 cut(s) 163, 373, 426
AspS9I GGNCC 3 cut(s) 154, 176, 256
AsuC2I CCSGG 2 cut(s) 518, 519
AsuHPI GGTGA 2 cut(s) 27, 91
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 2 cut(s) 154, 256
BanI GGYRCC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 366
BbsI GAAGAC 2 cut(s) 198, 602
BbvI GCAGC 4 cut(s) 196, 406, 784, 787
BccI CCATC 5 cut(s) 61, 92, 129, 347, 394
BceAI ACGGC 1 cut(s) 759
BciVI GTATCC 1 cut(s) 261
BcnI CCSGG 2 cut(s) 518, 519
BcoDI GTCTC 2 cut(s) 221, 535
BfmI CTRYAG 1 cut(s) 710
BfoI RGCGCY 1 cut(s) 374
BfuI GTATCC 1 cut(s) 261
Bme1390I CCNGG 2 cut(s) 518, 519
Bme18I GGWCC 2 cut(s) 154, 256
BmeT110I CYCGRG 1 cut(s) 517
BmgT120I GGNCC 3 cut(s) 154, 176, 256
BmiI GGNNCC 4 cut(s) 20, 178, 258, 372
BmrFI CCNGG 2 cut(s) 518, 519
BmsI GCATC 1 cut(s) 705
BpiI GAAGAC 2 cut(s) 198, 602
BpmI CTGGAG 1 cut(s) 5
BpuMI CCSGG 2 cut(s) 518, 519
BsaHI GRCGYC 2 cut(s) 230, 371
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 2 cut(s) 517, 518
BsaWI WCCGGW 3 cut(s) 151, 253, 412
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 163, 380, 519, 649
Bse118I RCCGGY 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 253
BseDI CCNNGG 2 cut(s) 517, 518
BseGI GGATG 3 cut(s) 121, 220, 789
BseLI CCNNNNNNNGG 5 cut(s) 151, 163, 380, 519, 649
BseMII CTCAG 1 cut(s) 257
BseRI GAGGAG 2 cut(s) 100, 452
BseXI GCAGC 4 cut(s) 196, 406, 784, 787
BsgI GTGCAG 2 cut(s) 413, 600
Bsh1236I CGCG 2 cut(s) 161, 426
BshFI GGCC 3 cut(s) 120, 167, 177
BshNI GGYRCC 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 5 cut(s) 117, 152, 254, 413, 518
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 5 cut(s) 151, 163, 380, 519, 649
BsmAI GTCTC 2 cut(s) 221, 535
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 584
BsnI GGCC 3 cut(s) 120, 167, 177
Bso31I GGTCTC 1 cut(s) 535
BsoBI CYCGRG 1 cut(s) 517
Bsp1286I GDGCHC 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 342
BspANI GGCC 3 cut(s) 120, 167, 177
BspCNI CTCAG 1 cut(s) 258
BspEI TCCGGA 1 cut(s) 253
BspFNI CGCG 2 cut(s) 161, 426
BspLI GGNNCC 4 cut(s) 20, 178, 258, 372
BspT107I GGYRCC 1 cut(s) 370
BspTNI GGTCTC 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 342
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 2 cut(s) 517, 518
BssNI GRCGYC 2 cut(s) 230, 371
Bst4CI ACNGT 2 cut(s) 553, 595
Bst6I CTCTTC 1 cut(s) 492
BstACI GRCGYC 2 cut(s) 230, 371
BstAUI TGTACA 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 118, 165
BstDEI CTNAG 1 cut(s) 266
BstF5I GGATG 3 cut(s) 121, 220, 789
BstFNI CGCG 2 cut(s) 161, 426
BstH2I RGCGCY 1 cut(s) 374
BstHHI GCGC 3 cut(s) 163, 373, 426
BstMAI GTCTC 2 cut(s) 221, 535
BstMWI GCNNNNNNNGC 3 cut(s) 11, 209, 370
BstSCI CCNGG 2 cut(s) 516, 517
BstSFI CTRYAG 1 cut(s) 710
BstUI CGCG 2 cut(s) 161, 426
BstV1I GCAGC 4 cut(s) 196, 406, 784, 787
BstV2I GAAGAC 2 cut(s) 198, 602
BsuI GTATCC 1 cut(s) 261
BsuRI GGCC 3 cut(s) 120, 167, 177
BtsCI GGATG 3 cut(s) 121, 220, 789
BtsIMutI CAGTG 1 cut(s) 583
Cac8I GCNNGC 2 cut(s) 118, 165
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 3 cut(s) 163, 373, 426
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 154, 176, 256
Cfr9I CCCGGG 1 cut(s) 517
CseI GACGC 2 cut(s) 238, 572
Csp6I GTAC 1 cut(s) 343
CviAII CATG 1 cut(s) 632
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 1 cut(s) 266
DinI GGCGCC 1 cut(s) 372
DrdI GACNNNNNNGTC 1 cut(s) 753
DseDI GACNNNNNNGTC 1 cut(s) 753
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
EciI GGCGGA 2 cut(s) 103, 121
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 535
Eco47I GGWCC 2 cut(s) 154, 256
Eco57I CTGAAG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 517
EcoO109I RGGNCCY 1 cut(s) 176
EcoT38I GRGCYC 1 cut(s) 366
EgeI GGCGCC 1 cut(s) 372
EheI GGCGCC 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 1 cut(s) 635
FaiI YATR 6 cut(s) 263, 338, 528, 633, 732, 820
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 1 cut(s) 584
FatI CATG 1 cut(s) 631
FauI CCCGC 1 cut(s) 88
FokI GGATG 3 cut(s) 108, 227, 796
FriOI GRGCYC 1 cut(s) 366
GlaI GCGC 3 cut(s) 162, 372, 425
GsuI CTGGAG 1 cut(s) 5
HaeII RGCGCY 1 cut(s) 374
HaeIII GGCC 3 cut(s) 120, 167, 177
HapII CCGG 5 cut(s) 117, 152, 254, 413, 518
HgaI GACGC 2 cut(s) 238, 572
HhaI GCGC 3 cut(s) 163, 373, 426
Hin1I GRCGYC 2 cut(s) 230, 371
Hin1II CATG 1 cut(s) 635
Hin6I GCGC 3 cut(s) 161, 371, 424
HinP1I GCGC 3 cut(s) 161, 371, 424
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 3 cut(s) 290, 384, 454
HpaII CCGG 5 cut(s) 117, 152, 254, 413, 518
HphI GGTGA 2 cut(s) 27, 91
Hpy166II GTNNAC 3 cut(s) 157, 591, 598
Hpy188I TCNGA 2 cut(s) 540, 703
Hpy188III TCNNGA 3 cut(s) 254, 689, 758
Hpy8I GTNNAC 3 cut(s) 157, 591, 598
HpyAV CCTTC 5 cut(s) 115, 425, 523, 674, 787
HpyCH4III ACNGT 2 cut(s) 553, 595
HpyCH4V TGCA 3 cut(s) 394, 617, 649
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 209, 370
HpyF3I CTNAG 1 cut(s) 266
Hsp92I GRCGYC 2 cut(s) 230, 371
Hsp92II CATG 1 cut(s) 635
HspAI GCGC 3 cut(s) 161, 371, 424
KasI GGCGCC 1 cut(s) 370
Kpn2I TCCGGA 1 cut(s) 253
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
LmnI GCTCC 2 cut(s) 24, 421
Lsp1109I GCAGC 4 cut(s) 196, 406, 784, 787
LweI GCATC 1 cut(s) 705
MboII GAAGA 7 cut(s) 79, 203, 322, 509, 602, 736, 746
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 8 cut(s) 27, 72, 221, 240, 285, 304, 314, 635
Mly113I GGCGCC 1 cut(s) 371
MmeI TCCRAC 1 cut(s) 361
MroI TCCGGA 1 cut(s) 253
MroNI GCCGGC 1 cut(s) 116
MroXI GAANNNNTTC 1 cut(s) 684
MspA1I CMGCKG 2 cut(s) 235, 397
MspI CCGG 5 cut(s) 117, 152, 254, 413, 518
MspR9I CCNGG 2 cut(s) 518, 519
MvnI CGCG 2 cut(s) 161, 426
MwoI GCNNNNNNNGC 3 cut(s) 11, 209, 370
NaeI GCCGGC 1 cut(s) 118
NarI GGCGCC 1 cut(s) 371
NciI CCSGG 2 cut(s) 518, 519
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 635
NlaIV GGNNCC 4 cut(s) 20, 178, 258, 372
NmeAIII GCCGAG 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 482
PdiI GCCGGC 1 cut(s) 118
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 3 cut(s) 290, 384, 454
PluTI GGCGCC 1 cut(s) 374
PspN4I GGNNCC 4 cut(s) 20, 178, 258, 372
PspPI GGNCC 3 cut(s) 154, 176, 256
PstNI CAGNNNCTG 1 cut(s) 551
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
Sau96I GGNCC 3 cut(s) 154, 176, 256
ScrFI CCNGG 2 cut(s) 518, 519
SduI GDGCHC 1 cut(s) 366
SetI ASST 6 cut(s) 107, 205, 399, 590, 610, 711
SfaNI GCATC 1 cut(s) 705
SfcI CTRYAG 1 cut(s) 710
SfoI GGCGCC 1 cut(s) 372
SinI GGWCC 2 cut(s) 154, 256
SmaI CCCGGG 1 cut(s) 519
Sse9I AATT 8 cut(s) 27, 72, 221, 240, 285, 304, 314, 635
SspDI GGCGCC 1 cut(s) 370
StyD4I CCNGG 2 cut(s) 516, 517
TaaI ACNGT 2 cut(s) 553, 595
TasI AATT 8 cut(s) 27, 72, 221, 240, 285, 304, 314, 635
TatI WGTACW 1 cut(s) 342
TauI GCSGC 7 cut(s) 14, 66, 69, 137, 235, 376, 429
TfiI GAWTC 3 cut(s) 290, 384, 454
TscAI CASTG 1 cut(s) 583
TseI GCWGC 4 cut(s) 209, 394, 797, 800
TspDTI ATGAA 4 cut(s) 315, 648, 747, 800
TspMI CCCGGG 1 cut(s) 517
TspRI CASTG 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 154, 256
XapI RAATTY 3 cut(s) 221, 304, 635
XmaI CCCGGG 1 cut(s) 517
XmnI GAANNNNTTC 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.