Rh2DG117900

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001719
Physical Location & Seq
Forward (+)
9771436 .. 9771957
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG117900.1

Sequence Viewer

Length: 522 bp
ATGGCTGGGGCAGTTCTTTGTACACTAAATGCTCGGCTTGATTCAGCTATGATATCTGTTCTTCTTTGTCATTCTGAAGCTAAAATCGTCTTTGTCGATCACCAATTACTTGGAATTGTTGATGGAGCACTTGAATTAATTACCGAAAAAGCTGTTTCAAAACCGCCTATTGTAGTTTTAATTGCTGATTCTGATGGATCATCATCTCCAAACAGCTCTACTTCAGAAACTGAACCACCTTATGAGTATGAAAGTCTTTTAGAAAGTGGAAATAGTGGATTTGAGATTAGGAGACCAAAAAGTGAATGGGATCCTATCAGTGTAAATTACACCTCTGGCACAACATCAAGGCCGAAAGGGGTTGTCTATAATCACCGCGGCGCTTATCTCAATTCACTGGCCACAGTTTTGCTTTGCAGAATAGGCTCAATGCCGGTTAATCTATGGACTGCGCCTATGTTTCATTGCCATGGATGGTCTCTCATTTGGGGTTTGGCTGCTCAGGGTGGTACTAAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

173

Amino Acids

18.65

Weight (kDa)

5.68

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 1 - 171 2.8e-25 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 378
AciI CCGC 3 cut(s) 164, 376, 378
AclWI GGATC 3 cut(s) 205, 305, 318
AcoI YGGCCR 1 cut(s) 399
AcuI CTGAAG 2 cut(s) 96, 207
AfaI GTAC 3 cut(s) 22, 511, 518
AgsI TTSAA 2 cut(s) 134, 159
AluBI AGCT 4 cut(s) 47, 80, 152, 216
AluI AGCT 4 cut(s) 47, 80, 152, 216
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 2 cut(s) 286, 483
AlwI GGATC 3 cut(s) 205, 305, 318
AlwNI CAGNNNCTG 1 cut(s) 230
AoxI GGCC 2 cut(s) 350, 399
ApeKI GCWGC 1 cut(s) 497
AseI ATTAAT 1 cut(s) 137
AspLEI GCGC 2 cut(s) 383, 454
AsuHPI GGTGA 2 cut(s) 92, 365
BalI TGGCCA 1 cut(s) 401
BamHI GGATCC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 1 cut(s) 484
BccI CCATC 3 cut(s) 116, 188, 468
BcoDI GTCTC 2 cut(s) 286, 483
BfoI RGCGCY 1 cut(s) 384
BisI GCNGC 2 cut(s) 379, 498
BlsI GCNGC 2 cut(s) 380, 499
BmcAI AGTACT 1 cut(s) 518
BmiI GGNNCC 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 501
BsaBI GATNNNNATC 1 cut(s) 202
BsaI GGTCTC 2 cut(s) 286, 483
BsaJI CCNNGG 2 cut(s) 376, 469
Bse118I RCCGGY 1 cut(s) 433
Bse1I ACTGG 1 cut(s) 402
Bse3DI GCAATG 1 cut(s) 463
Bse8I GATNNNNATC 1 cut(s) 202
BseDI CCNNGG 2 cut(s) 376, 469
BseGI GGATG 1 cut(s) 479
BseJI GATNNNNATC 1 cut(s) 202
BseMI GCAATG 1 cut(s) 463
BseMII CTCAG 1 cut(s) 515
BseNI ACTGG 1 cut(s) 402
BseXI GCAGC 1 cut(s) 484
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 2 cut(s) 352, 401
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 434
BsmAI GTCTC 2 cut(s) 286, 483
BsnI GGCC 2 cut(s) 352, 401
Bso31I GGTCTC 2 cut(s) 286, 483
Bsp1286I GDGCHC 1 cut(s) 130
Bsp1407I TGTACA 1 cut(s) 20
Bsp143I GATC 3 cut(s) 97, 197, 310
Bsp19I CCATGG 1 cut(s) 469
BspACI CCGC 3 cut(s) 164, 376, 378
BspANI GGCC 2 cut(s) 352, 401
BspCNI CTCAG 1 cut(s) 514
BspFNI CGCG 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 3 cut(s) 205, 305, 318
BspTNI GGTCTC 2 cut(s) 286, 483
BsrDI GCAATG 1 cut(s) 463
BsrFI RCCGGY 1 cut(s) 433
BsrGI TGTACA 1 cut(s) 20
BsrI ACTGG 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 376, 469
BssMI GATC 3 cut(s) 97, 197, 310
BssT1I CCWWGG 1 cut(s) 469
Bst4CI ACNGT 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 20
BstDEI CTNAG 2 cut(s) 501, 513
BstDSI CCRYGG 2 cut(s) 376, 469
BstF5I GGATG 1 cut(s) 479
BstFNI CGCG 1 cut(s) 378
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 2 cut(s) 383, 454
BstKTI GATC 3 cut(s) 100, 200, 313
BstMAI GTCTC 2 cut(s) 286, 483
BstMBI GATC 3 cut(s) 97, 197, 310
BstMWI GCNNNNNNNGC 1 cut(s) 423
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 310
BstXI CCANNNNNNTGG 1 cut(s) 110
BstYI RGATCY 1 cut(s) 310
BsuRI GGCC 2 cut(s) 352, 401
BtgI CCRYGG 2 cut(s) 376, 469
BtsCI GGATG 1 cut(s) 479
BtsIMutI CAGTG 2 cut(s) 325, 395
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 2 cut(s) 383, 454
Cfr10I RCCGGY 1 cut(s) 433
Cfr42I CCGCGG 1 cut(s) 379
Csp6I GTAC 3 cut(s) 21, 510, 517
CviAII CATG 1 cut(s) 470
CviQI GTAC 3 cut(s) 21, 510, 517
DdeI CTNAG 2 cut(s) 501, 513
DpnI GATC 3 cut(s) 99, 199, 312
DpnII GATC 3 cut(s) 97, 197, 310
EaeI YGGCCR 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 469
Eco31I GGTCTC 2 cut(s) 286, 483
Eco32I GATATC 1 cut(s) 54
Eco57I CTGAAG 2 cut(s) 96, 207
EcoRV GATATC 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 469
FaeI CATG 1 cut(s) 473
FaiI YATR 7 cut(s) 50, 243, 249, 369, 445, 458, 471
FatI CATG 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 379, 498
FokI GGATG 1 cut(s) 486
Fsp4HI GCNGC 2 cut(s) 379, 498
GlaI GCGC 2 cut(s) 382, 453
GluI GCNGC 2 cut(s) 379, 498
GsaI CCCAGC 1 cut(s) 9
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 2 cut(s) 352, 401
HapII CCGG 1 cut(s) 434
HhaI GCGC 2 cut(s) 383, 454
Hin1II CATG 1 cut(s) 473
Hin6I GCGC 2 cut(s) 381, 452
HinP1I GCGC 2 cut(s) 381, 452
HinfI GANTC 2 cut(s) 41, 188
HpaII CCGG 1 cut(s) 434
HphI GGTGA 2 cut(s) 92, 365
Hpy166II GTNNAC 1 cut(s) 23
Hpy188I TCNGA 3 cut(s) 76, 193, 226
Hpy8I GTNNAC 1 cut(s) 23
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4V TGCA 1 cut(s) 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 423
HpyF3I CTNAG 2 cut(s) 501, 513
Hsp92II CATG 1 cut(s) 473
HspAI GCGC 2 cut(s) 381, 452
KspI CCGCGG 1 cut(s) 379
Kzo9I GATC 3 cut(s) 97, 197, 310
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 4 cut(s) 321, 383, 447, 488
Lsp1109I GCAGC 1 cut(s) 484
MalI GATC 3 cut(s) 99, 199, 312
MboI GATC 3 cut(s) 97, 197, 310
MboII GAAGA 1 cut(s) 53
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 130
MlsI TGGCCA 1 cut(s) 401
MluCI AATT 7 cut(s) 104, 114, 134, 138, 180, 325, 391
MluNI TGGCCA 1 cut(s) 401
MnlI CCTC 1 cut(s) 343
Mox20I TGGCCA 1 cut(s) 401
MscI TGGCCA 1 cut(s) 401
MseI TTAA 3 cut(s) 137, 179, 438
MslI CAYNNNNRTG 1 cut(s) 468
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 378
MspI CCGG 1 cut(s) 434
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 1 cut(s) 423
NcoI CCATGG 1 cut(s) 469
NdeII GATC 3 cut(s) 97, 197, 310
NlaIII CATG 1 cut(s) 473
NlaIV GGNNCC 1 cut(s) 312
NmeAIII GCCGAG 1 cut(s) 13
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 2 cut(s) 41, 188
PkrI GCNGC 2 cut(s) 380, 499
PshBI ATTAAT 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 1 cut(s) 310
RsaI GTAC 3 cut(s) 22, 511, 518
RsaNI GTAC 3 cut(s) 21, 510, 517
RseI CAYNNNNRTG 1 cut(s) 468
SacII CCGCGG 1 cut(s) 379
SaqAI TTAA 3 cut(s) 137, 179, 438
SatI GCNGC 2 cut(s) 379, 498
Sau3AI GATC 3 cut(s) 97, 197, 310
ScaI AGTACT 1 cut(s) 518
SduI GDGCHC 1 cut(s) 130
SetI ASST 6 cut(s) 49, 82, 154, 218, 241, 335
Sfr303I CCGCGG 1 cut(s) 379
SgrBI CCGCGG 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 468
Sse9I AATT 7 cut(s) 104, 114, 134, 138, 180, 325, 391
SsiI CCGC 3 cut(s) 164, 376, 378
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 1 cut(s) 406
TaqI TCGA 1 cut(s) 96
TasI AATT 7 cut(s) 104, 114, 134, 138, 180, 325, 391
TatI WGTACW 2 cut(s) 20, 516
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 2 cut(s) 41, 188
Tru1I TTAA 3 cut(s) 137, 179, 438
Tru9I TTAA 3 cut(s) 137, 179, 438
TscAI CASTG 2 cut(s) 325, 402
TseI GCWGC 1 cut(s) 497
TspDTI ATGAA 2 cut(s) 264, 452
TspRI CASTG 2 cut(s) 325, 402
VspI ATTAAT 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 303
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.