RchiOBHm_Chr2g0159461

Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007539
Physical Location & Seq
Forward (+)
75571933 .. 75572719
787 bp
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UTR
Exon/CDS
Intron
PRQ52803

Sequence Viewer

Length: 333 bp
ATGGGCGTGAGGATGGTTTATGTTTTCCACGGCACATTGCTAGTGAATTCGGCCGGCCACATATGGGGATATCAAGCATGGAAGACCAGTGATCTGTCTAAGAATCTCTGGTGGTTGGCATTGGTTGCATTTGGAGAAGGGTGGCACAACAATCACCATGCTTTCGAATATTCGGCTCGACAGGGCCTCGAATGGTGGCAGTTTGACCTGACTTGGTACATCATAAAATTTCTTGAAGCTCTAGGGTTGGCAACTGATGTGAAAGTACCATCTGAGGCTCATAAGAAACGTAAAGCTTTGGAGACCAAAACAACCATGGCTGCCATGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FA_desaturase

Protein Analysis

110

Amino Acids

12.77

Weight (kDa)

9.26

Isoelectric Point (pI)

26.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 4 - 75 9.3e-07 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 51, 55
AcsI RAATTY 2 cut(s) 46, 227
AfaI GTAC 2 cut(s) 218, 267
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 1 cut(s) 236
AluBI AGCT 2 cut(s) 239, 296
AluI AGCT 2 cut(s) 239, 296
Alw26I GTCTC 1 cut(s) 296
AoxI GGCC 3 cut(s) 51, 55, 184
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 2 cut(s) 46, 227
AspS9I GGNCC 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 146
AsuII TTCGAA 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 89
BbvI GCAGC 1 cut(s) 307
BccI CCATC 2 cut(s) 7, 277
BceAI ACGGC 1 cut(s) 46
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 2 cut(s) 41, 242
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 184
BpiI GAAGAC 1 cut(s) 89
Bpu14I TTCGAA 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 28, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 87
Bse3DI GCAATG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 28, 315
BseGI GGATG 1 cut(s) 18
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 87
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 307
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 53, 57, 186
BsiEI CGRYCG 1 cut(s) 54
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 296
BsnI GGCC 3 cut(s) 53, 57, 186
Bso31I GGTCTC 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 165
Bsp143I GATC 1 cut(s) 91
Bsp19I CCATGG 1 cut(s) 315
BspANI GGCC 3 cut(s) 53, 57, 186
BspCNI CTCAG 1 cut(s) 265
BspT104I TTCGAA 1 cut(s) 165
BspTNI GGTCTC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 2 cut(s) 28, 315
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 2 cut(s) 99, 273
BstDSI CCRYGG 2 cut(s) 28, 315
BstF5I GGATG 1 cut(s) 18
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 1 cut(s) 91
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstV1I GCAGC 1 cut(s) 307
BstV2I GAAGAC 1 cut(s) 89
BstZI CGGCCG 1 cut(s) 51
BsuRI GGCC 3 cut(s) 53, 57, 186
BtgI CCRYGG 2 cut(s) 28, 315
BtsCI GGATG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 94
Cac8I GCNNGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 217, 266
CviAII CATG 4 cut(s) 78, 158, 316, 325
CviJI RGCY 8 cut(s) 53, 57, 176, 186, 239, 278, 296, 320
CviKI_1 RGCY 8 cut(s) 53, 57, 176, 186, 239, 278, 296, 320
CviQI GTAC 2 cut(s) 217, 266
DdeI CTNAG 2 cut(s) 99, 273
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
EaeI YGGCCR 2 cut(s) 51, 55
EagI CGGCCG 1 cut(s) 51
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 71
Eco52I CGGCCG 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 46
EcoRV GATATC 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 4 cut(s) 81, 161, 319, 328
FaiI YATR 9 cut(s) 21, 62, 64, 79, 159, 224, 282, 317, 326
FatI CATG 4 cut(s) 77, 157, 315, 324
FauNDI CATATG 1 cut(s) 62
Fnu4HI GCNGC 1 cut(s) 321
FokI GGATG 1 cut(s) 25
FseI GGCCGGCC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 2 cut(s) 41, 242
GluI GCNGC 1 cut(s) 321
HaeIII GGCC 3 cut(s) 53, 57, 186
HapII CCGG 1 cut(s) 54
Hin1II CATG 4 cut(s) 81, 161, 319, 328
HindIII AAGCTT 1 cut(s) 294
HinfI GANTC 1 cut(s) 103
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 146
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 1 cut(s) 233
HpyAV CCTTC 1 cut(s) 131
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 1 cut(s) 128
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 2 cut(s) 99, 273
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 4 cut(s) 81, 161, 319, 328
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 1 cut(s) 91
LpnPI CCDG 5 cut(s) 67, 94, 100, 167, 221
Lsp1109I GCAGC 1 cut(s) 307
MaeI CTAG 2 cut(s) 41, 242
MaeII ACGT 1 cut(s) 289
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 1 cut(s) 94
MluCI AATT 2 cut(s) 46, 227
MnlI CCTC 3 cut(s) 3, 197, 268
MroNI GCCGGC 1 cut(s) 53
MspI CCGG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 125
NaeI GCCGGC 1 cut(s) 55
NcoI CCATGG 1 cut(s) 315
NdeI CATATG 1 cut(s) 62
NdeII GATC 1 cut(s) 91
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 4 cut(s) 81, 161, 319, 328
NspV TTCGAA 1 cut(s) 165
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 1 cut(s) 322
PspPI GGNCC 1 cut(s) 184
RigI GGCCGGCC 1 cut(s) 57
RsaI GTAC 2 cut(s) 218, 267
RsaNI GTAC 2 cut(s) 217, 266
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 184
SetI ASST 4 cut(s) 210, 241, 292, 298
SfuI TTCGAA 1 cut(s) 165
Sse9I AATT 2 cut(s) 46, 227
SspI AATATT 1 cut(s) 170
SspMI CTAG 2 cut(s) 41, 242
StyI CCWWGG 1 cut(s) 315
TaiI ACGT 1 cut(s) 292
TaqI TCGA 3 cut(s) 165, 178, 189
TasI AATT 2 cut(s) 46, 227
TfiI GAWTC 1 cut(s) 103
TscAI CASTG 1 cut(s) 94
TseI GCWGC 1 cut(s) 320
TspRI CASTG 1 cut(s) 94
XapI RAATTY 2 cut(s) 46, 227
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XspI CTAG 2 cut(s) 41, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.