RLG00000015820

Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007539
Physical Location & Seq
Reverse (-)
1655273 .. 1657157
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015820

Sequence Viewer

Length: 564 bp
ATGAAATTTTTATGTAAACATAATATTTTGCTTCTATTGACAAGTAGGTCTAAATGGGGAAGCTATTATAGTGAACAATATGGAGGACTGAAGAATGTTGAAGATTTGAAAATGCAGCCATTCTATAGGTTTCTTCATCATACGTTTCTTCTACACTCGTTTATTCTTGGAGGAACTCTATATGCCCTTGGTGGGTTTCCCTTCATTGTTTGGGGAATGGGAGTGAGGATGCTAGTTGTTTTCCACGGCACCATGCTAGTAAATTCGGCCTGCCACACATGGGGGTATCAAGCATGGAATACCGGTGATCTATCTAAGAACCTTTGGTGGTTGGGAGTGCTTGGACTAGGAGAAGGATGGCACAGCAATCACCACGCTTTCGAATACTCGGCTCGACAAGGCCTCGAATGGTGGCAGATTGACGTGACTTGGTACGTCATAAAATTTCTTGAAGCTCTACGATTGGCAAAAGATGTGAAACTACCATCCGAAGATCATAAGAAACGCAAAGCTTTAGAGACCAGAACAACGATGGTTTCTCATGAAATCAGCCACGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FA_desaturase

Protein Analysis

188

Amino Acids

21.83

Weight (kDa)

8.82

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 49 - 141 8e-06 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 248
AcsI RAATTY 3 cut(s) 5, 262, 443
AcuI CTGAAG 1 cut(s) 110
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 192, 280
AgeI ACCGGT 1 cut(s) 302
AgsI TTSAA 3 cut(s) 101, 109, 452
AjiI CACGTC 1 cut(s) 424
AluBI AGCT 3 cut(s) 63, 455, 512
AluI AGCT 3 cut(s) 63, 455, 512
Alw26I GTCTC 1 cut(s) 512
AoxI GGCC 2 cut(s) 267, 400
ApeKI GCWGC 1 cut(s) 115
ApoI RAATTY 3 cut(s) 5, 262, 443
AsiGI ACCGGT 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 317, 362
AsuII TTCGAA 1 cut(s) 381
BanI GGYRCC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 127
BccI CCATC 3 cut(s) 351, 493, 526
BceAI ACGGC 1 cut(s) 262
BcoDI GTCTC 1 cut(s) 512
BfaI CTAG 3 cut(s) 233, 257, 347
BfmI CTRYAG 1 cut(s) 124
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
BmgBI CACGTC 1 cut(s) 424
BmiI GGNNCC 1 cut(s) 250
BmsI GCATC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 2 cut(s) 187, 244
BsaWI WCCGGW 1 cut(s) 302
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 280
Bse118I RCCGGY 1 cut(s) 302
BseDI CCNNGG 2 cut(s) 187, 244
BseGI GGATG 3 cut(s) 234, 362, 485
BseLI CCNNNNNNNGG 2 cut(s) 192, 280
BseXI GCAGC 1 cut(s) 127
BshFI GGCC 2 cut(s) 269, 402
BshNI GGYRCC 1 cut(s) 248
BshTI ACCGGT 1 cut(s) 302
BsiSI CCGG 1 cut(s) 303
BslI CCNNNNNNNGG 2 cut(s) 192, 280
BsmAI GTCTC 1 cut(s) 512
BsnI GGCC 2 cut(s) 269, 402
Bso31I GGTCTC 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 381
Bsp143I GATC 2 cut(s) 307, 493
BspANI GGCC 2 cut(s) 269, 402
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 1 cut(s) 250
BspT104I TTCGAA 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 2 cut(s) 187, 244
BssMI GATC 2 cut(s) 307, 493
BssT1I CCWWGG 1 cut(s) 187
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 1 cut(s) 315
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 3 cut(s) 234, 362, 485
BstKTI GATC 2 cut(s) 310, 496
BstMAI GTCTC 1 cut(s) 512
BstMBI GATC 2 cut(s) 307, 493
BstSFI CTRYAG 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 127
BsuRI GGCC 2 cut(s) 269, 402
BtgI CCRYGG 1 cut(s) 244
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 3 cut(s) 234, 362, 485
Cac8I GCNNGC 1 cut(s) 271
CciI TCATGA 1 cut(s) 541
Cfr10I RCCGGY 1 cut(s) 302
Csp6I GTAC 1 cut(s) 433
CspAI ACCGGT 1 cut(s) 302
CviAII CATG 4 cut(s) 253, 279, 294, 542
CviJI RGCY 8 cut(s) 63, 118, 269, 392, 402, 455, 512, 552
CviKI_1 RGCY 8 cut(s) 63, 118, 269, 392, 402, 455, 512, 552
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 1 cut(s) 315
DpnI GATC 2 cut(s) 309, 495
DpnII GATC 2 cut(s) 307, 493
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 187
Eco147I AGGCCT 1 cut(s) 402
Eco31I GGTCTC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 187
FaeI CATG 4 cut(s) 256, 282, 297, 545
FatI CATG 4 cut(s) 252, 278, 293, 541
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 3 cut(s) 241, 369, 472
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 3 cut(s) 233, 257, 347
GluI GCNGC 1 cut(s) 116
HaeIII GGCC 2 cut(s) 269, 402
HapII CCGG 1 cut(s) 303
Hin1II CATG 4 cut(s) 256, 282, 297, 545
HindIII AAGCTT 1 cut(s) 510
HpaII CCGG 1 cut(s) 303
HphI GGTGA 2 cut(s) 317, 362
Hpy166II GTNNAC 2 cut(s) 17, 74
Hpy188I TCNGA 1 cut(s) 490
Hpy188III TCNNGA 2 cut(s) 449, 542
Hpy8I GTNNAC 2 cut(s) 17, 74
HpyAV CCTTC 2 cut(s) 211, 347
HpyCH4IV ACGT 3 cut(s) 143, 423, 435
HpyCH4V TGCA 1 cut(s) 115
HpyF3I CTNAG 1 cut(s) 315
HpySE526I ACGT 3 cut(s) 143, 423, 435
Hsp92II CATG 4 cut(s) 256, 282, 297, 545
Kzo9I GATC 2 cut(s) 307, 493
LpnPI CCDG 3 cut(s) 283, 316, 535
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 219
MaeI CTAG 3 cut(s) 233, 257, 347
MaeII ACGT 3 cut(s) 143, 423, 435
MaeIII GTNAC 1 cut(s) 424
MalI GATC 2 cut(s) 309, 495
MboI GATC 2 cut(s) 307, 493
MboII GAAGA 5 cut(s) 103, 113, 125, 140, 503
MluCI AATT 3 cut(s) 5, 262, 443
MnlI CCTC 4 cut(s) 77, 164, 219, 413
MseI TTAA 1 cut(s) 562
MspI CCGG 1 cut(s) 303
NdeII GATC 2 cut(s) 307, 493
NlaIII CATG 4 cut(s) 256, 282, 297, 545
NlaIV GGNNCC 1 cut(s) 250
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 1 cut(s) 424
NspV TTCGAA 1 cut(s) 381
PagI TCATGA 1 cut(s) 541
PceI AGGCCT 1 cut(s) 402
PinAI ACCGGT 1 cut(s) 302
PkrI GCNGC 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 250
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
SaqAI TTAA 1 cut(s) 562
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 2 cut(s) 307, 493
SetI ASST 9 cut(s) 50, 65, 131, 146, 324, 426, 438, 457, 514
SfaNI GCATC 1 cut(s) 219
SfcI CTRYAG 1 cut(s) 124
SfuI TTCGAA 1 cut(s) 381
Sse9I AATT 3 cut(s) 5, 262, 443
SseBI AGGCCT 1 cut(s) 402
SspI AATATT 1 cut(s) 25
SspMI CTAG 3 cut(s) 233, 257, 347
StuI AGGCCT 1 cut(s) 402
StyI CCWWGG 1 cut(s) 187
TaiI ACGT 3 cut(s) 146, 426, 438
TaqI TCGA 3 cut(s) 381, 394, 405
TasI AATT 3 cut(s) 5, 262, 443
Tru1I TTAA 1 cut(s) 562
Tru9I TTAA 1 cut(s) 562
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 4 cut(s) 17, 125, 193, 558
XapI RAATTY 3 cut(s) 5, 262, 443
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XspI CTAG 3 cut(s) 233, 257, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.