RchiOBHm_Chr4g0425021

Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007539
Physical Location & Seq
Forward (+)
50670331 .. 50671792
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39426

Sequence Viewer

Length: 447 bp
ATGGATATGTTGTTAGTGGTTTCCAGGTTTTTGATGAATATATGGTACAAAGCGATTTCCAGTTTCTATATGAATGGTATATTGTGGTTTATATTTCCAAGTTCTGATGAATGTATGGTACATCATTGTGGTTTCCAGGGAGTGAGGACGGTATATGTTTTCCACCTCACCTTGCTAGTAAACTCGGCCGGTCACATCTGGGGAAAACAAGTATGGAACACCGGCGACCTGTCAAAGAACAATTGGTTGTTGGGATGGATCGCACTCGGAGAAGGGTGGCACAACAACCATCATGCATTTGAATACTCAGCTCGACAAGGTCTGGAATGGTGGCAAGTCGACTTGACTTGGTATGTAATCACAGCTCTTCAAGCAATTGGGTTGGCAACTGACGTCAAAGTTCCCACTGAATCTCAGATGCGTCGCAAAGCCCTCAAGAAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01040 ko01212 ko03320 ko04152 ko04212 map01040 map01212 map03320 map04152 map04212
Pfam Domains
Protein Families
FA_desaturase

Protein Analysis

148

Amino Acids

17.28

Weight (kDa)

8.59

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 28 - 120 3.9e-06 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 396
AccI GTMKAC 1 cut(s) 339
AclWI GGATC 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 186
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 2 cut(s) 47, 120
AgsI TTSAA 2 cut(s) 302, 371
AjnI CCWGG 2 cut(s) 23, 135
AluBI AGCT 2 cut(s) 311, 365
AluI AGCT 2 cut(s) 311, 365
AlwI GGATC 1 cut(s) 266
AoxI GGCC 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 160
BccI CCATC 2 cut(s) 249, 297
BciT130I CCWGG 2 cut(s) 25, 137
BfaI CTAG 1 cut(s) 176
Bme1390I CCNGG 2 cut(s) 25, 137
BmrFI CCNGG 2 cut(s) 25, 137
BmsI GCATC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 136
Bse118I RCCGGY 2 cut(s) 188, 221
Bse1I ACTGG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 25, 137
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 1 cut(s) 260
BseMII CTCAG 2 cut(s) 321, 428
BseNI ACTGG 1 cut(s) 60
BseX3I CGGCCG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 189
BsiSI CCGG 2 cut(s) 189, 222
BsnI GGCC 1 cut(s) 188
Bsp143I GATC 1 cut(s) 258
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 2 cut(s) 320, 427
BspPI GGATC 1 cut(s) 266
BspQI GCTCTTC 1 cut(s) 372
BsrFI RCCGGY 2 cut(s) 188, 221
BsrI ACTGG 1 cut(s) 60
BssAI RCCGGY 2 cut(s) 188, 221
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 1 cut(s) 258
BssNI GRCGYC 1 cut(s) 393
Bst2UI CCWGG 2 cut(s) 25, 137
Bst4CI ACNGT 1 cut(s) 151
Bst6I CTCTTC 1 cut(s) 372
BstACI GRCGYC 1 cut(s) 393
BstDEI CTNAG 2 cut(s) 307, 414
BstF5I GGATG 1 cut(s) 260
BstKTI GATC 1 cut(s) 261
BstMBI GATC 1 cut(s) 258
BstMCI CGRYCG 1 cut(s) 189
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstNI CCWGG 2 cut(s) 25, 137
BstSCI CCNGG 2 cut(s) 23, 135
BstZI CGGCCG 1 cut(s) 186
BsuRI GGCC 1 cut(s) 188
BtsCI GGATG 1 cut(s) 260
BtsIMutI CAGTG 1 cut(s) 405
Cfr10I RCCGGY 2 cut(s) 188, 221
CseI GACGC 1 cut(s) 410
Csp6I GTAC 2 cut(s) 46, 119
CviAII CATG 1 cut(s) 293
CviJI RGCY 4 cut(s) 188, 311, 365, 431
CviKI_1 RGCY 4 cut(s) 188, 311, 365, 431
CviQI GTAC 2 cut(s) 46, 119
DdeI CTNAG 2 cut(s) 307, 414
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
EaeI YGGCCR 1 cut(s) 186
EagI CGGCCG 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EclXI CGGCCG 1 cut(s) 186
Eco52I CGGCCG 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 23, 135
EcoT22I ATGCAT 1 cut(s) 298
FaeI CATG 1 cut(s) 296
FatI CATG 1 cut(s) 292
FblI GTMKAC 1 cut(s) 339
FokI GGATG 1 cut(s) 267
FspBI CTAG 1 cut(s) 176
HaeIII GGCC 1 cut(s) 188
HapII CCGG 2 cut(s) 189, 222
HgaI GACGC 1 cut(s) 410
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 1 cut(s) 296
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 1 cut(s) 410
HpaII CCGG 2 cut(s) 189, 222
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 181, 340
Hpy188I TCNGA 3 cut(s) 106, 269, 417
Hpy188III TCNNGA 2 cut(s) 323, 436
Hpy8I GTNNAC 2 cut(s) 181, 340
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 2 cut(s) 307, 414
HpySE526I ACGT 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 1 cut(s) 296
Kzo9I GATC 1 cut(s) 258
LguI GCTCTTC 1 cut(s) 372
LweI GCATC 1 cut(s) 408
MaeI CTAG 1 cut(s) 176
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 191
MalI GATC 1 cut(s) 260
MboI GATC 1 cut(s) 258
MboII GAAGA 1 cut(s) 359
MfeI CAATTG 2 cut(s) 241, 375
MluCI AATT 2 cut(s) 241, 375
MnlI CCTC 3 cut(s) 138, 176, 443
Mph1103I ATGCAT 1 cut(s) 298
MslI CAYNNNNRTG 1 cut(s) 126
MspI CCGG 2 cut(s) 189, 222
MspR9I CCNGG 2 cut(s) 25, 137
MunI CAATTG 2 cut(s) 241, 375
MvaI CCWGG 2 cut(s) 25, 137
MwoI GCNNNNNNNGC 1 cut(s) 371
NdeII GATC 1 cut(s) 258
NlaIII CATG 1 cut(s) 296
NmeAIII GCCGAG 1 cut(s) 164
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 410
PflFI GACNNNGTC 1 cut(s) 318
Psp6I CCWGG 2 cut(s) 23, 135
PspGI CCWGG 2 cut(s) 23, 135
PsyI GACNNNGTC 1 cut(s) 318
RsaI GTAC 2 cut(s) 47, 120
RsaNI GTAC 2 cut(s) 46, 119
RseI CAYNNNNRTG 1 cut(s) 126
SalI GTCGAC 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 372
Sau3AI GATC 1 cut(s) 258
ScrFI CCNGG 2 cut(s) 25, 137
SetI ASST 8 cut(s) 29, 168, 173, 231, 313, 322, 367, 396
SfaNI GCATC 1 cut(s) 408
SgrAI CRCCGGYG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 2 cut(s) 241, 375
SspI AATATT 1 cut(s) 443
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 2 cut(s) 23, 135
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 313, 339
TasI AATT 2 cut(s) 241, 375
TfiI GAWTC 1 cut(s) 410
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 3 cut(s) 50, 86, 123
TspRI CASTG 1 cut(s) 412
Tth111I GACNNNGTC 1 cut(s) 318
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 1 cut(s) 176
ZraI GACGTC 1 cut(s) 394
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.