RchiOBHm_Chr2g0164781

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002307
Physical Location & Seq
Forward (+)
79819254 .. 79820732
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53280

Sequence Viewer

Length: 1443 bp
ATGAAGTATGTGAAGTTGGCTTCCCATTTCTTGACAACCAACCTCTTGAAACTCTTGTTAATTCTTGTAATAGCAGTCATTGTAATTGAAGCCTCTCAGATGAATCTAGATCACATTCATCAGCTCCGACTCCAACTCTGTTATAATCTGGTTAGTGTCATCACTAGCTCTGCTATTCTTGTTTTGGCTTCCACCATTTACATTATGACTCGACCCAAATCGGTTTACCTCGTAGACTACTCGTGCTATAAGCCCCCTAACAATTTGAAAATGAACTACCAGAAGTTTATTAATCAGTCCAGATTGACCGGGGCCTTTGATGAGTCCTCCCTCGAGTTTCAGCACAAGATTCTTGAGCGGTCTGGCCTTGGAGAGCAGACATATCTCCCTGAAGCAATGCATTTCATTCCGCCGAGGCTGTCAATGGCGGCAGCGCGTGAGGAGGCGGAGCAGGTGATTTTTGGAGCGTTGGATAATCTATTTGCAAATACTAATGTCAATCCTAAAGACATTGGGATTGTTGTTATGAATTCTAGTCTGTTTAATCCAATTCCTTCATTATCTTCTATGATTGTTAATAAGTACAAATTGAGGGGAAGTATTAAGAGTTATAATTTGGGAGGTATGGGATGTAGTGCTGGAGTTATAGCTGTAGATCTTGCTAAGGACCTGTTGCAAGTTCAAAAGAACACTTATGCAGTTGTTGTTAGCACTGAGAATATTACTAAAAATGGGTACCTTGGGAACAAGAAATCCATGTTAGTACAAAATTGTTTGTTTCGTGTGGGTGGGTCTGCGGTTTTGCTCTCGAACAAATCATCTGATATGTGTAGGGCCAAGTACAAGCTTGTGCATATCGTGAGGAATCATATGGGCTCGAATGATAGGGCATATAGGTGTGCTTATCAAGAGGAGGACGATGCTGGCAAAGCTGGGGTTTCGTTGTCGAAGGAGCTCATGGCGATTGCTGGTGTAGCGCTCAAGACTAATATCACAGCTTTGCTCGGTCCTCTTGTCCTACCTATAAGCGACCAGCTTCTCTTCATTGCTACTGTCCTTGCAAACAAGTTGTTCAATGCGAAAGTCAACCCTTACATCCCGAATTTCAAACTCGTTTTCGATCATTTTTGCATCCATGCTGGTGGAAGGTCTGTGATTGATGAGCTTGAGAAGAATTTGAAGCTTCTACCAGTTCATGTTGAGGCGTCACGGATGACACTCCACAGGTTTGGTAACACCTCCTCTAGCTCGATTTGGTATGAGTTGGCTTACACCGAGGCAAAGGGGAGGATACAAAAGGGGAACCGTGTGTGGCAGATTGCGCTCGGAAGTGGATTCAAGTGTAACAGTGTAGTCTGGGAGGCTCTCCGGAAAGCGAAACCTTCTCCCAGTAGCCCTTGGGAAGATTGCATTCATAGGTATCCGGTGCAGACTGTTCTCTAG

Protein Analysis

480

Amino Acids

53.34

Weight (kDa)

9.36

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 67 - 356 3.5e-133 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 374 - 454 8.7e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 376 - 434 3.1e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 144, 612
AarI CACCTGC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 442
Acc65I GGTACC 1 cut(s) 735
AccB1I GGYRCC 1 cut(s) 735
AccBSI CCGCTC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 234
AccII CGCG 1 cut(s) 436
AccIII TCCGGA 1 cut(s) 1368
AciI CCGC 5 cut(s) 358, 410, 428, 446, 797
AcsI RAATTY 3 cut(s) 529, 1102, 1174
AcuI CTGAAG 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 1205
AfaI GTAC 4 cut(s) 584, 737, 765, 842
AfeI AGCGCT 1 cut(s) 978
AgsI TTSAA 8 cut(s) 49, 89, 268, 683, 1075, 1108, 1180, 1339
AloI GAACNNNNNNTCC 2 cut(s) 737, 769
Alw21I GWGCWC 1 cut(s) 957
Ama87I CYCGRG 1 cut(s) 332
Aor13HI TCCGGA 1 cut(s) 1368
Aor51HI AGCGCT 1 cut(s) 978
AoxI GGCC 3 cut(s) 312, 364, 834
ApeKI GCWGC 1 cut(s) 431
ApoI RAATTY 3 cut(s) 529, 1102, 1174
AseI ATTAAT 1 cut(s) 291
Asp718I GGTACC 1 cut(s) 735
AspLEI GCGC 3 cut(s) 436, 979, 1324
AspS9I GGNCC 4 cut(s) 312, 667, 834, 1007
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 466
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 2 cut(s) 667, 1007
BanI GGYRCC 1 cut(s) 735
BanII GRGCYC 2 cut(s) 878, 957
BauI CACGAG 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 957
BbvI GCAGC 1 cut(s) 443
BciVI GTATCC 2 cut(s) 1284, 1431
BcnI CCSGG 1 cut(s) 310
BfaI CTAG 5 cut(s) 107, 165, 534, 1245, 1441
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 1 cut(s) 980
BfuAI ACCTGC 1 cut(s) 442
BfuI GTATCC 2 cut(s) 1284, 1431
BglII AGATCT 1 cut(s) 655
BisI GCNGC 2 cut(s) 429, 432
BlsI GCNGC 2 cut(s) 430, 433
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 2 cut(s) 667, 1007
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 4 cut(s) 312, 667, 834, 1007
BmiI GGNNCC 3 cut(s) 313, 737, 1304
BmrFI CCNGG 1 cut(s) 310
BmrI ACTGGG 1 cut(s) 1383
BmsI GCATC 2 cut(s) 910, 1140
BmuI ACTGGG 1 cut(s) 1383
BpmI CTGGAG 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 663
BpuEI CTTGAG 3 cut(s) 374, 965, 1187
BpuMI CCSGG 1 cut(s) 310
BsaHI GRCGYC 1 cut(s) 1205
BsaJI CCNNGG 6 cut(s) 309, 367, 413, 739, 1275, 1397
BsaWI WCCGGW 2 cut(s) 1368, 1423
Bse1I ACTGG 2 cut(s) 1190, 1389
Bse3DI GCAATG 2 cut(s) 402, 1044
BseAI TCCGGA 1 cut(s) 1368
BseDI CCNNGG 6 cut(s) 309, 367, 413, 739, 1275, 1397
BseGI GGATG 4 cut(s) 635, 1095, 1131, 1218
BseMI GCAATG 2 cut(s) 402, 1044
BseMII CTCAG 2 cut(s) 110, 705
BseNI ACTGG 2 cut(s) 1190, 1389
BseRI GAGGAG 3 cut(s) 455, 926, 1231
BseXI GCAGC 1 cut(s) 443
BseYI CCCAGC 1 cut(s) 932
Bsh1236I CGCG 1 cut(s) 436
BshFI GGCC 3 cut(s) 314, 366, 836
BshNI GGYRCC 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 957
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 3 cut(s) 309, 1369, 1424
BsmI GAATGC 1 cut(s) 1410
BsnI GGCC 3 cut(s) 314, 366, 836
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 878, 957
Bsp13I TCCGGA 1 cut(s) 1368
Bsp143I GATC 3 cut(s) 109, 655, 1120
BspACI CCGC 5 cut(s) 358, 410, 428, 446, 797
BspANI GGCC 3 cut(s) 314, 366, 836
BspCNI CTCAG 2 cut(s) 109, 706
BspEI TCCGGA 1 cut(s) 1368
BspFNI CGCG 1 cut(s) 436
BspLI GGNNCC 3 cut(s) 313, 737, 1304
BspMI ACCTGC 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 735
BsrBI CCGCTC 1 cut(s) 358
BsrDI GCAATG 2 cut(s) 402, 1044
BsrI ACTGG 2 cut(s) 1190, 1389
BssECI CCNNGG 6 cut(s) 309, 367, 413, 739, 1275, 1397
BssMI GATC 3 cut(s) 109, 655, 1120
BssNI GRCGYC 1 cut(s) 1205
BssSI CACGAG 1 cut(s) 241
BssT1I CCWWGG 3 cut(s) 367, 739, 1397
Bst2BI CACGAG 1 cut(s) 241
Bst4CI ACNGT 4 cut(s) 1054, 1307, 1349, 1435
Bst6I CTCTTC 1 cut(s) 1046
BstACI GRCGYC 1 cut(s) 1205
BstC8I GCNNGC 1 cut(s) 925
BstDEI CTNAG 3 cut(s) 96, 663, 714
BstF5I GGATG 4 cut(s) 635, 1095, 1131, 1218
BstFNI CGCG 1 cut(s) 436
BstH2I RGCGCY 1 cut(s) 980
BstHHI GCGC 3 cut(s) 436, 979, 1324
BstKTI GATC 3 cut(s) 112, 658, 1123
BstMBI GATC 3 cut(s) 109, 655, 1120
BstMWI GCNNNNNNNGC 3 cut(s) 929, 974, 1321
BstSCI CCNGG 1 cut(s) 308
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 655
BstXI CCANNNNNNTGG 2 cut(s) 1142, 1229
BstYI RGATCY 1 cut(s) 655
BsuI GTATCC 2 cut(s) 1284, 1431
BsuRI GGCC 3 cut(s) 314, 366, 836
BtsCI GGATG 4 cut(s) 635, 1095, 1131, 1218
BtsIMutI CAGTG 2 cut(s) 711, 1354
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 925
CfoI GCGC 3 cut(s) 436, 979, 1324
Cfr13I GGNCC 4 cut(s) 312, 667, 834, 1007
CseI GACGC 1 cut(s) 1194
Csp6I GTAC 4 cut(s) 583, 736, 764, 841
CviAII CATG 4 cut(s) 757, 958, 1136, 1196
CviQI GTAC 4 cut(s) 583, 736, 764, 841
DdeI CTNAG 3 cut(s) 96, 663, 714
DpnI GATC 3 cut(s) 111, 657, 1122
DpnII GATC 3 cut(s) 109, 655, 1120
Eam1104I CTCTTC 1 cut(s) 1046
EarI CTCTTC 1 cut(s) 1046
EciI GGCGGA 2 cut(s) 399, 461
Ecl136II GAGCTC 1 cut(s) 955
Eco130I CCWWGG 3 cut(s) 367, 739, 1397
Eco24I GRGCYC 2 cut(s) 878, 957
Eco47I GGWCC 2 cut(s) 667, 1007
Eco47III AGCGCT 1 cut(s) 978
Eco53kI GAGCTC 1 cut(s) 955
Eco57I CTGAAG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 955
EcoO109I RGGNCCY 2 cut(s) 312, 667
EcoRI GAATTC 1 cut(s) 529
EcoT14I CCWWGG 3 cut(s) 367, 739, 1397
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 2 cut(s) 878, 957
ErhI CCWWGG 3 cut(s) 367, 739, 1397
FaeI CATG 4 cut(s) 760, 961, 1139, 1199
FatI CATG 4 cut(s) 756, 957, 1135, 1195
FauNDI CATATG 1 cut(s) 870
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 2 cut(s) 429, 432
FokI GGATG 4 cut(s) 642, 1082, 1118, 1225
FriOI GRGCYC 2 cut(s) 878, 957
Fsp4HI GCNGC 2 cut(s) 429, 432
FspBI CTAG 5 cut(s) 107, 165, 534, 1245, 1441
GlaI GCGC 3 cut(s) 435, 978, 1323
GluI GCNGC 2 cut(s) 429, 432
GsaI CCCAGC 1 cut(s) 936
GsuI CTGGAG 1 cut(s) 660
HaeII RGCGCY 1 cut(s) 980
HaeIII GGCC 3 cut(s) 314, 366, 836
HapII CCGG 3 cut(s) 309, 1369, 1424
HgaI GACGC 1 cut(s) 1194
HhaI GCGC 3 cut(s) 436, 979, 1324
Hin1I GRCGYC 1 cut(s) 1205
Hin1II CATG 4 cut(s) 760, 961, 1139, 1199
Hin6I GCGC 3 cut(s) 434, 977, 1322
HinP1I GCGC 3 cut(s) 434, 977, 1322
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HindIII AAGCTT 2 cut(s) 845, 1181
HinfI GANTC 7 cut(s) 103, 129, 208, 323, 349, 865, 1335
HpaII CCGG 3 cut(s) 309, 1369, 1424
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 3 cut(s) 226, 235, 1087
Hpy188I TCNGA 4 cut(s) 99, 128, 823, 1328
Hpy8I GTNNAC 3 cut(s) 226, 235, 1087
HpyAV CCTTC 4 cut(s) 564, 943, 1140, 1392
HpyCH4III ACNGT 4 cut(s) 1054, 1307, 1349, 1435
HpyCH4V TGCA 9 cut(s) 400, 485, 676, 698, 853, 1061, 1131, 1410, 1429
HpyF10VI GCNNNNNNNGC 3 cut(s) 929, 974, 1321
HpyF3I CTNAG 3 cut(s) 96, 663, 714
Hsp92I GRCGYC 1 cut(s) 1205
Hsp92II CATG 4 cut(s) 760, 961, 1139, 1199
HspAI GCGC 3 cut(s) 434, 977, 1322
Kpn2I TCCGGA 1 cut(s) 1368
KpnI GGTACC 1 cut(s) 739
Kzo9I GATC 3 cut(s) 109, 655, 1120
LmnI GCTCC 4 cut(s) 129, 448, 464, 952
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 2 cut(s) 910, 1140
MaeI CTAG 5 cut(s) 107, 165, 534, 1245, 1441
MaeIII GTNAC 3 cut(s) 1206, 1232, 1343
MalI GATC 3 cut(s) 111, 657, 1122
MbiI CCGCTC 1 cut(s) 358
MboI GATC 3 cut(s) 109, 655, 1120
MboII GAAGA 4 cut(s) 555, 1033, 1183, 1415
MflI RGATCY 1 cut(s) 655
MhlI GDGCHC 2 cut(s) 878, 957
MlyI GAGTC 3 cut(s) 123, 202, 332
MmeI TCCRAC 3 cut(s) 151, 157, 450
Mph1103I ATGCAT 1 cut(s) 402
MroI TCCGGA 1 cut(s) 1368
MseI TTAA 5 cut(s) 59, 291, 543, 576, 603
MslI CAYNNNNRTG 2 cut(s) 895, 1140
MspI CCGG 3 cut(s) 309, 1369, 1424
MspR9I CCNGG 1 cut(s) 310
Mva1269I GAATGC 1 cut(s) 1410
MvnI CGCG 1 cut(s) 436
MwoI GCNNNNNNNGC 3 cut(s) 929, 974, 1321
NciI CCSGG 1 cut(s) 310
NdeI CATATG 1 cut(s) 870
NdeII GATC 3 cut(s) 109, 655, 1120
NlaIII CATG 4 cut(s) 760, 961, 1139, 1199
NlaIV GGNNCC 3 cut(s) 313, 737, 1304
NmeAIII GCCGAG 1 cut(s) 438
NmuCI GTSAC 1 cut(s) 1206
NsiI ATGCAT 1 cut(s) 402
PaeR7I CTCGAG 1 cut(s) 332
PaqCI CACCTGC 1 cut(s) 442
PctI GAATGC 1 cut(s) 1410
PfeI GAWTC 4 cut(s) 103, 349, 865, 1335
PkrI GCNGC 2 cut(s) 430, 433
PleI GAGTC 3 cut(s) 123, 202, 331
PpsI GAGTC 3 cut(s) 123, 202, 331
PpuMI RGGWCCY 1 cut(s) 667
PshBI ATTAAT 1 cut(s) 291
PsiI TTATAA 2 cut(s) 144, 612
Psp124BI GAGCTC 1 cut(s) 957
Psp5II RGGWCCY 1 cut(s) 667
PspFI CCCAGC 1 cut(s) 932
PspN4I GGNNCC 3 cut(s) 313, 737, 1304
PspPI GGNCC 4 cut(s) 312, 667, 834, 1007
PspPPI RGGWCCY 1 cut(s) 667
PspXI VCTCGAGB 1 cut(s) 332
PsuI RGATCY 1 cut(s) 655
RsaI GTAC 4 cut(s) 584, 737, 765, 842
RsaNI GTAC 4 cut(s) 583, 736, 764, 841
RseI CAYNNNNRTG 2 cut(s) 895, 1140
SacI GAGCTC 1 cut(s) 957
SaqAI TTAA 5 cut(s) 59, 291, 543, 576, 603
SatI GCNGC 2 cut(s) 429, 432
Sau3AI GATC 3 cut(s) 109, 655, 1120
Sau96I GGNCC 4 cut(s) 312, 667, 834, 1007
SchI GAGTC 3 cut(s) 123, 202, 332
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 2 cut(s) 878, 957
SfaNI GCATC 2 cut(s) 910, 1140
SfcI CTRYAG 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 332
SinI GGWCC 2 cut(s) 667, 1007
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 2 cut(s) 895, 1140
SmlI CTYRAG 4 cut(s) 332, 353, 980, 1166
SmoI CTYRAG 4 cut(s) 332, 353, 980, 1166
SsiI CCGC 5 cut(s) 358, 410, 428, 446, 797
SspI AATATT 1 cut(s) 721
SspMI CTAG 5 cut(s) 107, 165, 534, 1245, 1441
SstI GAGCTC 1 cut(s) 957
StyD4I CCNGG 1 cut(s) 308
StyI CCWWGG 3 cut(s) 367, 739, 1397
TaaI ACNGT 4 cut(s) 1054, 1307, 1349, 1435
TaqI TCGA 7 cut(s) 211, 333, 809, 878, 947, 1119, 1250
TaqII GACCGA 1 cut(s) 995
TatI WGTACW 3 cut(s) 582, 763, 840
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 4 cut(s) 103, 349, 865, 1335
Tru1I TTAA 5 cut(s) 59, 291, 543, 576, 603
Tru9I TTAA 5 cut(s) 59, 291, 543, 576, 603
TscAI CASTG 2 cut(s) 718, 1354
TseFI GTSAC 1 cut(s) 1206
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 1 cut(s) 1206
TspGWI ACGGA 1 cut(s) 1225
TspRI CASTG 2 cut(s) 718, 1354
VpaK11BI GGWCC 2 cut(s) 667, 1007
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 3 cut(s) 529, 1102, 1174
XbaI TCTAGA 1 cut(s) 106
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 5 cut(s) 107, 165, 534, 1245, 1441
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.