RchiOBHm_Chr6g0264551

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002307
Physical Location & Seq
Forward (+)
19749234 .. 19751657
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23727

Sequence Viewer

Length: 1530 bp
ATGAGCTCTGAGTCTGTTGACAATGGTGGAGCTCAGAACATTCAGATCCACCAAGGCCGTAGAGTCCCAGATTTCTTGAACAGTGTCAACTTGAAGTATGTGAAGCTGGGTTACCATTACTTGATAACCAATCTTCTAAAGCTCTGCTTGATTCCTCTAATGGGGATCATTGTTATTGAAGCCGCTCAGATGAACCCGGGTGACATTCACCAGCTCTGGCTCCAACTCCAGTATAACCTGGTCAGTGTCATCACTTTCTCTGCTGTTCTCGTTTTCGGATCCACTGTCTACATTATGACCCGACCCAGATCCGTTTACCTTGTGGACTATTCCTGTTACAAGCCCCCAGACCATTTGAAAGTGAAATACCACAAGTTTATGGACCATTCAAGATTGATTGGGGATTTTGATGAGTCCTCGCTCGAGTTTCAGCGCAAGATTCTTGAGAGGTCTGGCCTTGGAGAGGAGACCTATCTTCCTGAAGGAATGCATTCCATTCCGCCGAGGCCGACGATGGCGGCGGCGCGTGAGGAGGCAGAGCAGGTGATTTTTGGTGCATTGGATAATCTGTTTGCTAATACAAATATTAAGCCTAAGGACATTGGGATTCTTGTTGTGAATTGTAGCTTGTTTAACCCAACTCCTTCATTATCTGCTATGATTGTTAACAAGTATAAGTTGAGGGGTAACATTAGGAGTTTTAATTTGGGAGGTATGGGGTGTAGTGCTGGAGTTATAGCTGTTGATCTTGCTAAGGACATGTTGCAAGTTCATAGGAATACTTATGCAGTTGTTGTCAGCACTGAGAATATCACTCAGAATTGGTACTTTGGGAATAAGAAATCCATGTTGATACCGAATTGTTTGTTTCGTGTTGGTGGGTCTGCGGTTTTGCTCTCGAACAAGTCATTGGATAAGCGTCTGGCTAAGTATAAGCTTGTTCATGTTGTGAGGACTCATAAAGGGGCGGATGAGAAGGCGTTTAGGTGTGTTTATCAAGAGCAGGATGATATTGGCAAAACTGGGGTTTCTTTGTCAAAGGAACTCATGGCAATTGCTGGTGGAGCTCTTAAGACTAACATCACAACATTGGGTCCCCTTGTTCTGCCTCTAAGTGAGCAGCTTCTCTTCTTTGCTACTTTGGTTGCAAAGAAGCTGTTCAATGCAAAAGTCAAGCCTTATATCCCAGATTTTAAGCTTGCTTTCGATCATTTCTGCATACATGCTGGTGGAAGAGCTGTGATTGATGAGCTTGAGAAGAATCTGCAGCTTCTGCCAGTTCATGTTGAGGCGTCTCGGATGACACTCCACAGGTTTGGTAATACATCATCCAGTTCAATTTGGTATGAGTTGGCTTACACTGAGGCAAAGGGGAGGATGCAAAAGGGGAACCGTGTCTGGCAGATTGCATTTGGAAGCGGTTTCAAGTGTAACAGTGCTGTCTGGGAGGCTCTCCATAATGTAAAACCGTCTCCTAGTGGTCCATGGGAAGATTGCATTCACAACTATCCAGTGCAGATCATTGTTTAG

Protein Analysis

509

Amino Acids

56.9

Weight (kDa)

9.1

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 97 - 386 2.1e-145 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 403 - 483 2.7e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 405 - 477 5.3e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 532
Acc36I ACCTGC 1 cut(s) 532
AccBSI CCGCTC 1 cut(s) 185
AccI GTMKAC 1 cut(s) 288
AccII CGCG 1 cut(s) 526
AciI CCGC 7 cut(s) 183, 500, 518, 521, 887, 968, 1419
AclWI GGATC 5 cut(s) 40, 173, 273, 286, 303
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 1292
AfaI GTAC 1 cut(s) 827
AfiI CCNNNNNNNGG 2 cut(s) 161, 463
AflII CTTAAG 1 cut(s) 1070
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 8 cut(s) 79, 94, 179, 358, 390, 1162, 1338, 1426
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
Alw21I GWGCWC 3 cut(s) 8, 34, 1069
Alw26I GTCTC 3 cut(s) 461, 1299, 1476
AlwI GGATC 5 cut(s) 40, 173, 273, 286, 303
AlwNI CAGNNNCTG 1 cut(s) 1273
Ama87I CYCGRG 2 cut(s) 196, 422
AoxI GGCC 3 cut(s) 55, 454, 506
ApeKI GCWGC 2 cut(s) 1120, 1267
Asp700I GAANNNNTTC 2 cut(s) 490, 1157
AspLEI GCGC 2 cut(s) 435, 526
AspS9I GGNCC 3 cut(s) 382, 1094, 1481
AsuC2I CCSGG 2 cut(s) 197, 198
AsuHPI GGTGA 3 cut(s) 200, 212, 556
AvaI CYCGRG 2 cut(s) 196, 422
AvaII GGWCC 3 cut(s) 382, 1094, 1481
AxyI CCTNAGG 1 cut(s) 594
BamHI GGATCC 1 cut(s) 278
BanII GRGCYC 3 cut(s) 8, 34, 1069
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
Bbv12I GWGCWC 3 cut(s) 8, 34, 1069
BbvI GCAGC 2 cut(s) 1132, 1279
BccI CCATC 1 cut(s) 508
BceAI ACGGC 1 cut(s) 42
BciT130I CCWGG 1 cut(s) 239
BcnI CCSGG 2 cut(s) 197, 198
BcoDI GTCTC 3 cut(s) 461, 1299, 1476
BfaI CTAG 1 cut(s) 1476
BfmI CTRYAG 1 cut(s) 1265
BfrI CTTAAG 1 cut(s) 1070
BfuAI ACCTGC 1 cut(s) 532
BisI GCNGC 5 cut(s) 183, 519, 522, 1121, 1268
BlsI GCNGC 5 cut(s) 184, 520, 523, 1122, 1269
Bme1390I CCNGG 3 cut(s) 197, 198, 239
Bme18I GGWCC 3 cut(s) 382, 1094, 1481
BmeT110I CYCGRG 2 cut(s) 196, 422
BmgT120I GGNCC 3 cut(s) 382, 1094, 1481
BmiI GGNNCC 5 cut(s) 221, 280, 1095, 1096, 1391
BmrFI CCNGG 3 cut(s) 197, 198, 239
BmrI ACTGGG 1 cut(s) 1032
BmsI GCATC 1 cut(s) 1368
BmuI ACTGGG 1 cut(s) 1032
BpmI CTGGAG 2 cut(s) 212, 750
Bpu10I CCTNAGC 1 cut(s) 753
BpuEI CTTGAG 2 cut(s) 464, 1274
BpuMI CCSGG 2 cut(s) 197, 198
BsaHI GRCGYC 1 cut(s) 1292
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 5 cut(s) 52, 196, 457, 503, 1484
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 463
Bse1I ACTGG 5 cut(s) 229, 1027, 1277, 1332, 1511
Bse21I CCTNAGG 1 cut(s) 594
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 5 cut(s) 52, 196, 457, 503, 1484
BseGI GGATG 5 cut(s) 976, 1012, 1305, 1328, 1383
BseLI CCNNNNNNNGG 2 cut(s) 161, 463
BseMII CTCAG 5 cut(s) 47, 200, 795, 830, 1353
BseNI ACTGG 5 cut(s) 229, 1027, 1277, 1332, 1511
BseRI GAGGAG 2 cut(s) 479, 545
BseXI GCAGC 2 cut(s) 1132, 1279
BseYI CCCAGC 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 526
BshFI GGCC 3 cut(s) 57, 456, 508
BsiHKAI GWGCWC 3 cut(s) 8, 34, 1069
BsiHKCI CYCGRG 2 cut(s) 196, 422
BsiSI CCGG 1 cut(s) 197
BslFI GGGAC 2 cut(s) 50, 1080
BslI CCNNNNNNNGG 2 cut(s) 161, 463
BsmAI GTCTC 3 cut(s) 461, 1299, 1476
BsmBI CGTCTC 2 cut(s) 1299, 1476
BsmFI GGGAC 2 cut(s) 50, 1080
BsmI GAATGC 3 cut(s) 490, 492, 1497
BsnI GGCC 3 cut(s) 57, 456, 508
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 2 cut(s) 196, 422
Bsp1286I GDGCHC 3 cut(s) 8, 34, 1069
Bsp143I GATC 7 cut(s) 45, 165, 278, 308, 745, 1207, 1518
Bsp19I CCATGG 1 cut(s) 1484
BspACI CCGC 7 cut(s) 183, 500, 518, 521, 887, 968, 1419
BspANI GGCC 3 cut(s) 57, 456, 508
BspCNI CTCAG 5 cut(s) 46, 199, 796, 829, 1354
BspFNI CGCG 1 cut(s) 526
BspLI GGNNCC 5 cut(s) 221, 280, 1095, 1096, 1391
BspMAI CTGCAG 1 cut(s) 1269
BspMI ACCTGC 1 cut(s) 532
BspPI GGATC 5 cut(s) 40, 173, 273, 286, 303
BspQI GCTCTTC 1 cut(s) 1228
BspTI CTTAAG 1 cut(s) 1070
BspTNI GGTCTC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 185
BsrI ACTGG 5 cut(s) 229, 1027, 1277, 1332, 1511
BssECI CCNNGG 5 cut(s) 52, 196, 457, 503, 1484
BssMI GATC 7 cut(s) 45, 165, 278, 308, 745, 1207, 1518
BssNI GRCGYC 1 cut(s) 1292
BssT1I CCWWGG 3 cut(s) 52, 457, 1484
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 5 cut(s) 83, 286, 1394, 1436, 1470
Bst6I CTCTTC 2 cut(s) 1133, 1228
BstACI GRCGYC 1 cut(s) 1292
BstAFI CTTAAG 1 cut(s) 1070
BstAPI GCANNNNNTGC 1 cut(s) 1273
BstC8I GCNNGC 1 cut(s) 1200
BstDSI CCRYGG 1 cut(s) 1484
BstEII GGTNACC 1 cut(s) 110
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 5 cut(s) 976, 1012, 1305, 1328, 1383
BstFNI CGCG 1 cut(s) 526
BstHHI GCGC 2 cut(s) 435, 526
BstKTI GATC 7 cut(s) 48, 168, 281, 311, 748, 1210, 1521
BstMAI GTCTC 3 cut(s) 461, 1299, 1476
BstMBI GATC 7 cut(s) 45, 165, 278, 308, 745, 1207, 1518
BstMWI GCNNNNNNNGC 2 cut(s) 1064, 1273
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 2 cut(s) 763, 1226
BstPI GGTNACC 1 cut(s) 110
BstSCI CCNGG 3 cut(s) 195, 196, 237
BstSFI CTRYAG 1 cut(s) 1265
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 2 cut(s) 1132, 1279
BstX2I RGATCY 3 cut(s) 45, 278, 308
BstXI CCANNNNNNTGG 1 cut(s) 1316
BstYI RGATCY 3 cut(s) 45, 278, 308
Bsu36I CCTNAGG 1 cut(s) 594
BsuRI GGCC 3 cut(s) 57, 456, 508
BtgI CCRYGG 1 cut(s) 1484
BtsCI GGATG 5 cut(s) 976, 1012, 1305, 1328, 1383
BtsIMutI CAGTG 7 cut(s) 88, 250, 282, 801, 1359, 1441, 1518
BveI ACCTGC 1 cut(s) 532
Cac8I GCNNGC 1 cut(s) 1200
CaiI CAGNNNCTG 1 cut(s) 1273
CfoI GCGC 2 cut(s) 435, 526
Cfr13I GGNCC 3 cut(s) 382, 1094, 1481
Cfr9I CCCGGG 1 cut(s) 196
CseI GACGC 2 cut(s) 908, 1281
CsiI ACCWGGT 1 cut(s) 237
Csp6I GTAC 1 cut(s) 826
CviAII CATG 7 cut(s) 760, 847, 944, 1048, 1223, 1283, 1485
CviQI GTAC 1 cut(s) 826
DpnI GATC 7 cut(s) 47, 167, 280, 310, 747, 1209, 1520
DpnII GATC 7 cut(s) 45, 165, 278, 308, 745, 1207, 1518
Eam1104I CTCTTC 2 cut(s) 1133, 1228
EarI CTCTTC 2 cut(s) 1133, 1228
EciI GGCGGA 2 cut(s) 489, 983
Ecl136II GAGCTC 3 cut(s) 6, 32, 1067
Eco130I CCWWGG 3 cut(s) 52, 457, 1484
Eco24I GRGCYC 3 cut(s) 8, 34, 1069
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 3 cut(s) 382, 1094, 1481
Eco53kI GAGCTC 3 cut(s) 6, 32, 1067
Eco57I CTGAAG 1 cut(s) 501
Eco81I CCTNAGG 1 cut(s) 594
Eco88I CYCGRG 2 cut(s) 196, 422
Eco91I GGTNACC 1 cut(s) 110
EcoICRI GAGCTC 3 cut(s) 6, 32, 1067
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 1094
EcoO65I GGTNACC 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 3 cut(s) 52, 457, 1484
EcoT22I ATGCAT 1 cut(s) 492
EcoT38I GRGCYC 3 cut(s) 8, 34, 1069
ErhI CCWWGG 3 cut(s) 52, 457, 1484
Esp3I CGTCTC 2 cut(s) 1299, 1476
FaeI CATG 7 cut(s) 763, 850, 947, 1051, 1226, 1286, 1488
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 2 cut(s) 50, 1080
FatI CATG 7 cut(s) 759, 846, 943, 1047, 1222, 1282, 1484
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 5 cut(s) 183, 519, 522, 1121, 1268
FokI GGATG 5 cut(s) 983, 1019, 1312, 1315, 1390
FriOI GRGCYC 3 cut(s) 8, 34, 1069
Fsp4HI GCNGC 5 cut(s) 183, 519, 522, 1121, 1268
FspBI CTAG 1 cut(s) 1476
GlaI GCGC 2 cut(s) 434, 525
GluI GCNGC 5 cut(s) 183, 519, 522, 1121, 1268
GsaI CCCAGC 1 cut(s) 110
GsuI CTGGAG 2 cut(s) 212, 750
HaeIII GGCC 3 cut(s) 57, 456, 508
HapII CCGG 1 cut(s) 197
HgaI GACGC 2 cut(s) 908, 1281
HhaI GCGC 2 cut(s) 435, 526
Hin1I GRCGYC 1 cut(s) 1292
Hin1II CATG 7 cut(s) 763, 850, 947, 1051, 1226, 1286, 1488
Hin6I GCGC 2 cut(s) 433, 524
HinP1I GCGC 2 cut(s) 433, 524
HincII GTYRAC 3 cut(s) 19, 88, 667
HindII GTYRAC 3 cut(s) 19, 88, 667
HindIII AAGCTT 2 cut(s) 935, 1196
HinfI GANTC 8 cut(s) 11, 63, 151, 413, 439, 607, 955, 1261
HpaI GTTAAC 1 cut(s) 667
HpaII CCGG 1 cut(s) 197
HphI GGTGA 3 cut(s) 200, 212, 556
Hpy166II GTNNAC 6 cut(s) 19, 88, 289, 316, 325, 667
Hpy188I TCNGA 7 cut(s) 10, 36, 45, 189, 278, 819, 1299
Hpy188III TCNNGA 6 cut(s) 76, 390, 443, 479, 898, 998
Hpy8I GTNNAC 6 cut(s) 19, 88, 289, 316, 325, 667
Hpy99I CGWCG 1 cut(s) 514
HpyAV CCTTC 3 cut(s) 476, 654, 970
HpyCH4III ACNGT 5 cut(s) 83, 286, 1394, 1436, 1470
HpyF10VI GCNNNNNNNGC 2 cut(s) 1064, 1273
Hsp92I GRCGYC 1 cut(s) 1292
Hsp92II CATG 7 cut(s) 763, 850, 947, 1051, 1226, 1286, 1488
HspAI GCGC 2 cut(s) 433, 524
KflI GGGWCCC 1 cut(s) 1094
KspAI GTTAAC 1 cut(s) 667
Kzo9I GATC 7 cut(s) 45, 165, 278, 308, 745, 1207, 1518
LguI GCTCTTC 1 cut(s) 1228
LmnI GCTCC 3 cut(s) 29, 225, 1064
Lsp1109I GCAGC 2 cut(s) 1132, 1279
LweI GCATC 1 cut(s) 1368
MabI ACCWGGT 1 cut(s) 237
MaeI CTAG 1 cut(s) 1476
MaeIII GTNAC 5 cut(s) 110, 200, 335, 686, 1430
MalI GATC 7 cut(s) 47, 167, 280, 310, 747, 1209, 1520
MbiI CCGCTC 1 cut(s) 185
MboI GATC 7 cut(s) 45, 165, 278, 308, 745, 1207, 1518
MboII GAAGA 6 cut(s) 125, 467, 1120, 1245, 1270, 1502
MfeI CAATTG 1 cut(s) 1053
MflI RGATCY 3 cut(s) 45, 278, 308
MhlI GDGCHC 3 cut(s) 8, 34, 1069
MluCI AATT 6 cut(s) 619, 703, 820, 859, 1053, 1338
MlyI GAGTC 4 cut(s) 20, 72, 422, 949
MmeI TCCRAC 1 cut(s) 247
Mph1103I ATGCAT 1 cut(s) 492
MroXI GAANNNNTTC 2 cut(s) 490, 1157
MseI TTAA 6 cut(s) 588, 633, 666, 702, 1071, 1194
MslI CAYNNNNRTG 1 cut(s) 1227
MspCI CTTAAG 1 cut(s) 1070
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 3 cut(s) 197, 198, 239
MunI CAATTG 1 cut(s) 1053
Mva1269I GAATGC 3 cut(s) 490, 492, 1497
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 2 cut(s) 1064, 1273
NciI CCSGG 2 cut(s) 197, 198
NcoI CCATGG 1 cut(s) 1484
NdeII GATC 7 cut(s) 45, 165, 278, 308, 745, 1207, 1518
NlaIII CATG 7 cut(s) 763, 850, 947, 1051, 1226, 1286, 1488
NlaIV GGNNCC 5 cut(s) 221, 280, 1095, 1096, 1391
NmeAIII GCCGAG 1 cut(s) 528
NmuCI GTSAC 1 cut(s) 200
NsiI ATGCAT 1 cut(s) 492
NspI RCATGY 2 cut(s) 763, 1226
PaeR7I CTCGAG 1 cut(s) 422
PaqCI CACCTGC 1 cut(s) 532
PciI ACATGT 1 cut(s) 759
PciSI GCTCTTC 1 cut(s) 1228
PctI GAATGC 3 cut(s) 490, 492, 1497
PdmI GAANNNNTTC 2 cut(s) 490, 1157
PfeI GAWTC 4 cut(s) 151, 439, 607, 1261
PkrI GCNGC 5 cut(s) 184, 520, 523, 1122, 1269
PleI GAGTC 4 cut(s) 19, 71, 421, 949
PpsI GAGTC 4 cut(s) 19, 71, 421, 949
PpuMI RGGWCCY 1 cut(s) 1094
PscI ACATGT 1 cut(s) 759
Psp124BI GAGCTC 3 cut(s) 8, 34, 1069
Psp5II RGGWCCY 1 cut(s) 1094
Psp6I CCWGG 1 cut(s) 237
PspEI GGTNACC 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 5 cut(s) 221, 280, 1095, 1096, 1391
PspPI GGNCC 3 cut(s) 382, 1094, 1481
PspPPI RGGWCCY 1 cut(s) 1094
PspXI VCTCGAGB 1 cut(s) 422
PstI CTGCAG 1 cut(s) 1269
PstNI CAGNNNCTG 1 cut(s) 1273
PsuI RGATCY 3 cut(s) 45, 278, 308
RsaI GTAC 1 cut(s) 827
RsaNI GTAC 1 cut(s) 826
RseI CAYNNNNRTG 1 cut(s) 1227
SacI GAGCTC 3 cut(s) 8, 34, 1069
SapI GCTCTTC 1 cut(s) 1228
SaqAI TTAA 6 cut(s) 588, 633, 666, 702, 1071, 1194
SatI GCNGC 5 cut(s) 183, 519, 522, 1121, 1268
Sau3AI GATC 7 cut(s) 45, 165, 278, 308, 745, 1207, 1518
Sau96I GGNCC 3 cut(s) 382, 1094, 1481
SchI GAGTC 4 cut(s) 20, 72, 422, 949
ScrFI CCNGG 3 cut(s) 197, 198, 239
SduI GDGCHC 3 cut(s) 8, 34, 1069
SexAI ACCWGGT 1 cut(s) 237
SfaNI GCATC 1 cut(s) 1368
SfcI CTRYAG 1 cut(s) 1265
Sfr274I CTCGAG 1 cut(s) 422
SinI GGWCC 3 cut(s) 382, 1094, 1481
SlaI CTCGAG 1 cut(s) 422
SmaI CCCGGG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 1227
SmlI CTYRAG 4 cut(s) 422, 443, 1070, 1253
SmoI CTYRAG 4 cut(s) 422, 443, 1070, 1253
Sse9I AATT 6 cut(s) 619, 703, 820, 859, 1053, 1338
SsiI CCGC 7 cut(s) 183, 500, 518, 521, 887, 968, 1419
SspI AATATT 1 cut(s) 586
SspMI CTAG 1 cut(s) 1476
SstI GAGCTC 3 cut(s) 8, 34, 1069
StyD4I CCNGG 3 cut(s) 195, 196, 237
StyI CCWWGG 3 cut(s) 52, 457, 1484
TaaI ACNGT 5 cut(s) 83, 286, 1394, 1436, 1470
TaqI TCGA 3 cut(s) 423, 899, 1206
TasI AATT 6 cut(s) 619, 703, 820, 859, 1053, 1338
TauI GCSGC 3 cut(s) 185, 521, 524
TfiI GAWTC 4 cut(s) 151, 439, 607, 1261
Tru1I TTAA 6 cut(s) 588, 633, 666, 702, 1071, 1194
Tru9I TTAA 6 cut(s) 588, 633, 666, 702, 1071, 1194
TscAI CASTG 7 cut(s) 88, 250, 289, 808, 1366, 1441, 1518
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 2 cut(s) 1120, 1267
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 5 cut(s) 206, 636, 761, 932, 1271
TspGWI ACGGA 1 cut(s) 301
TspMI CCCGGG 1 cut(s) 196
TspRI CASTG 7 cut(s) 88, 250, 289, 808, 1366, 1441, 1518
Vha464I CTTAAG 1 cut(s) 1070
VpaK11BI GGWCC 3 cut(s) 382, 1094, 1481
XagI CCTNNNNNAGG 1 cut(s) 461
XceI RCATGY 2 cut(s) 763, 1226
XhoI CTCGAG 1 cut(s) 422
XmaI CCCGGG 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 288
XmnI GAANNNNTTC 2 cut(s) 490, 1157
XspI CTAG 1 cut(s) 1476
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.