Rw0G009730

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00387
OG0002307
Physical Location & Seq
Reverse (-)
11201 .. 13448
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G009730.1

Sequence Viewer

Length: 1512 bp
ATGAGCTCTGGAGCGCAGAACATTCAGATCCACCAAGGCCGTAGACTCCCAGATTTCTTGAACAGTGTCAACTTGAAGTATGTGAAGTTGGGTTACCATTACCTGATAACCAATCTTCTAAAGCTCTGCTTGATTCCTCTAATGGGGATCATTGTTATTGAAGCCGCTCAGATGAACCCGGATGACATTCACGAGCTCTGGCTCCAACTCCAGTATAATCTGGTCAGTGTCATCACTTTCTCTGCTGTTCTCGTTTTCGGATCCACTGTCTACATTATGACCCGACCCAGATCCGTTTACCTTGTGGACTATTCCTGTTACAAGCCCCCAGACCATTTGAAAGTGAAATACCACAAGTTTATGGACCATTCAAGATTGATTGGGGATTTTGATGAGTCCTCGCTCGAGTTTCAGCGCAAGATTCTTGAGAGGTCTGGCCTTGGAGAGGAGACCTATCTTCCTGAAGGAATGCATTCCATTCCGCCGAGGCCGACGATGGCGGCGGCGCGTGAGGAGGCAGAGCAGGTGATTTTTGGTGCATTGGATAATCTGTTTGCTAATACAAATATTAAGCCTAAGGACATTGGGATTCTTGTTGTGAATTGTAGCTTGTTTAACCCAACTCCTTCATTATCTGCTATGATTGTTAATAAGTATAAGTTGAGGGGTAACATTAGGAGTTTTAATTTGGGAGGTATGGGGTGTAGTGCTGGAGTTATAGCTGTTGATCTTGCTAAGGACATGTTGCAAGTTCATAGGAATACTTATGCAGTTGTTGTCAGCACTGAGAATATCACTCAGAATTGGTACTTTGGGAATAAGAAATCCATGTTGATACCAAATTGTTTGTTTCGTGTTGGTGGGTCTGCGGTTTTGCTCTCGAACAAGTCATTGGATAAGCGTCTGGCTAAGTATAAGCTTGTTCATGTTGTGAGGACTCATAAAGGGGCGGATGAGAAGGCGTTTAGGTGTGTTTATCAAGAGCAGGATGATATTGGCAAAACTGGGGTTTCTTTGTCAAAGGAACTCATGGCAATTGCTGGTGGAGCTCTTAAGACTAACATCACAACATTGGGTCCCCTTGTTCTGCCTCTAAGTGAGCAGCTTCTCTTCTTTGCTACTTTGGTTGCAAAGAAGCTGTTCAATGCAAAAGTCAAGCCTTATATCCCAGATTTTAAGCTTGCTTTCGATCATTTCTGCATACATGCTGGTGGAAGAGCTGTGATTGATGAGCTTGAGAAGAATCTGCAGCTTCTGCCAGTTCATGTTGAGGCGTCTCGGATGACACTCCACAGGTTTGGTAATACATCATCCAGTTCAATTTGGTATGAGTTGGCTTACACTGAGGCAAAGGGGAGGATGCAAAAGGGGAACCGTGTCTGGCAGATTGCATTTGGAAGCGGTTTCAAGTGTAACAGTGCTGTCTGGGAGGCTCTCCATAATGTAAAACCGTCTCCTAGTGGTCCATGGGAAGATTGCATTCACAACTATCCAGTGCAGAACATTGTTTAG

Protein Analysis

503

Amino Acids

56.37

Weight (kDa)

9.1

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 91 - 380 2.1e-145 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 397 - 477 2.7e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 399 - 471 5.2e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 514
Acc36I ACCTGC 1 cut(s) 514
AccBSI CCGCTC 1 cut(s) 167
AccI GTMKAC 2 cut(s) 43, 270
AccII CGCG 1 cut(s) 508
AciI CCGC 7 cut(s) 165, 482, 500, 503, 869, 950, 1401
AclWI GGATC 5 cut(s) 22, 155, 255, 268, 285
AcuI CTGAAG 1 cut(s) 483
AcyI GRCGYC 1 cut(s) 1274
AfaI GTAC 1 cut(s) 809
AfiI CCNNNNNNNGG 2 cut(s) 143, 445
AflII CTTAAG 1 cut(s) 1052
AflIII ACRYGT 1 cut(s) 741
AgsI TTSAA 8 cut(s) 61, 76, 161, 340, 372, 1144, 1320, 1408
AjuI GAANNNNNNNTTGG 2 cut(s) 875, 907
Alw21I GWGCWC 3 cut(s) 8, 198, 1051
Alw26I GTCTC 3 cut(s) 443, 1281, 1458
AlwI GGATC 5 cut(s) 22, 155, 255, 268, 285
AlwNI CAGNNNCTG 1 cut(s) 1255
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 3 cut(s) 37, 436, 488
ApeKI GCWGC 2 cut(s) 1102, 1249
Asp700I GAANNNNTTC 2 cut(s) 472, 1139
AspLEI GCGC 3 cut(s) 16, 417, 508
AspS9I GGNCC 3 cut(s) 364, 1076, 1463
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 538
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 3 cut(s) 364, 1076, 1463
AxyI CCTNAGG 1 cut(s) 576
BamHI GGATCC 1 cut(s) 260
BanII GRGCYC 3 cut(s) 8, 198, 1051
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BauI CACGAG 1 cut(s) 191
Bbv12I GWGCWC 3 cut(s) 8, 198, 1051
BbvI GCAGC 2 cut(s) 1114, 1261
BccI CCATC 1 cut(s) 490
BceAI ACGGC 1 cut(s) 24
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 3 cut(s) 443, 1281, 1458
BfaI CTAG 1 cut(s) 1458
BfmI CTRYAG 1 cut(s) 1247
BfrI CTTAAG 1 cut(s) 1052
BfuAI ACCTGC 1 cut(s) 514
BisI GCNGC 5 cut(s) 165, 501, 504, 1103, 1250
BlsI GCNGC 5 cut(s) 166, 502, 505, 1104, 1251
Bme1390I CCNGG 1 cut(s) 179
Bme18I GGWCC 3 cut(s) 364, 1076, 1463
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 364, 1076, 1463
BmiI GGNNCC 5 cut(s) 203, 262, 1077, 1078, 1373
BmrFI CCNGG 1 cut(s) 179
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 1 cut(s) 1350
BmuI ACTGGG 1 cut(s) 1014
BpmI CTGGAG 3 cut(s) 30, 194, 732
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 2 cut(s) 446, 1256
BpuMI CCSGG 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 1274
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 4 cut(s) 34, 439, 485, 1466
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 445
Bse1I ACTGG 5 cut(s) 211, 1009, 1259, 1314, 1493
Bse21I CCTNAGG 1 cut(s) 576
BseDI CCNNGG 4 cut(s) 34, 439, 485, 1466
BseGI GGATG 6 cut(s) 187, 958, 994, 1287, 1310, 1365
BseLI CCNNNNNNNGG 2 cut(s) 143, 445
BseMII CTCAG 4 cut(s) 182, 777, 812, 1335
BseNI ACTGG 5 cut(s) 211, 1009, 1259, 1314, 1493
BseRI GAGGAG 2 cut(s) 461, 527
BseXI GCAGC 2 cut(s) 1114, 1261
Bsh1236I CGCG 1 cut(s) 508
BshFI GGCC 3 cut(s) 39, 438, 490
BsiHKAI GWGCWC 3 cut(s) 8, 198, 1051
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 1 cut(s) 179
BslFI GGGAC 1 cut(s) 1062
BslI CCNNNNNNNGG 2 cut(s) 143, 445
BsmAI GTCTC 3 cut(s) 443, 1281, 1458
BsmBI CGTCTC 2 cut(s) 1281, 1458
BsmFI GGGAC 1 cut(s) 1062
BsmI GAATGC 3 cut(s) 472, 474, 1479
BsnI GGCC 3 cut(s) 39, 438, 490
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 404
Bsp1286I GDGCHC 3 cut(s) 8, 198, 1051
Bsp143I GATC 6 cut(s) 27, 147, 260, 290, 727, 1189
Bsp19I CCATGG 1 cut(s) 1466
BspACI CCGC 7 cut(s) 165, 482, 500, 503, 869, 950, 1401
BspANI GGCC 3 cut(s) 39, 438, 490
BspCNI CTCAG 4 cut(s) 181, 778, 811, 1336
BspFNI CGCG 1 cut(s) 508
BspLI GGNNCC 5 cut(s) 203, 262, 1077, 1078, 1373
BspMAI CTGCAG 1 cut(s) 1251
BspMI ACCTGC 1 cut(s) 514
BspPI GGATC 5 cut(s) 22, 155, 255, 268, 285
BspQI GCTCTTC 1 cut(s) 1210
BspTI CTTAAG 1 cut(s) 1052
BspTNI GGTCTC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 167
BsrI ACTGG 5 cut(s) 211, 1009, 1259, 1314, 1493
BssECI CCNNGG 4 cut(s) 34, 439, 485, 1466
BssMI GATC 6 cut(s) 27, 147, 260, 290, 727, 1189
BssNI GRCGYC 1 cut(s) 1274
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 3 cut(s) 34, 439, 1466
Bst2BI CACGAG 1 cut(s) 191
Bst4CI ACNGT 5 cut(s) 65, 268, 1376, 1418, 1452
Bst6I CTCTTC 2 cut(s) 1115, 1210
BstACI GRCGYC 1 cut(s) 1274
BstAFI CTTAAG 1 cut(s) 1052
BstAPI GCANNNNNTGC 1 cut(s) 1255
BstC8I GCNNGC 1 cut(s) 1182
BstDEI CTNAG 8 cut(s) 168, 576, 735, 786, 798, 909, 1094, 1344
BstDSI CCRYGG 1 cut(s) 1466
BstEII GGTNACC 1 cut(s) 92
BstENI CCTNNNNNAGG 1 cut(s) 443
BstF5I GGATG 6 cut(s) 187, 958, 994, 1287, 1310, 1365
BstFNI CGCG 1 cut(s) 508
BstHHI GCGC 3 cut(s) 16, 417, 508
BstKTI GATC 6 cut(s) 30, 150, 263, 293, 730, 1192
BstMAI GTCTC 3 cut(s) 443, 1281, 1458
BstMBI GATC 6 cut(s) 27, 147, 260, 290, 727, 1189
BstMWI GCNNNNNNNGC 2 cut(s) 1046, 1255
BstNSI RCATGY 2 cut(s) 745, 1208
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 1247
BstUI CGCG 1 cut(s) 508
BstV1I GCAGC 2 cut(s) 1114, 1261
BstX2I RGATCY 3 cut(s) 27, 260, 290
BstXI CCANNNNNNTGG 1 cut(s) 1298
BstYI RGATCY 3 cut(s) 27, 260, 290
Bsu36I CCTNAGG 1 cut(s) 576
BsuRI GGCC 3 cut(s) 39, 438, 490
BtgI CCRYGG 1 cut(s) 1466
BtsCI GGATG 6 cut(s) 187, 958, 994, 1287, 1310, 1365
BtsIMutI CAGTG 7 cut(s) 70, 232, 264, 783, 1341, 1423, 1500
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 1 cut(s) 1182
CaiI CAGNNNCTG 1 cut(s) 1255
CfoI GCGC 3 cut(s) 16, 417, 508
Cfr13I GGNCC 3 cut(s) 364, 1076, 1463
CseI GACGC 2 cut(s) 890, 1263
Csp6I GTAC 1 cut(s) 808
CviAII CATG 7 cut(s) 742, 829, 926, 1030, 1205, 1265, 1467
CviQI GTAC 1 cut(s) 808
DdeI CTNAG 8 cut(s) 168, 576, 735, 786, 798, 909, 1094, 1344
DpnI GATC 6 cut(s) 29, 149, 262, 292, 729, 1191
DpnII GATC 6 cut(s) 27, 147, 260, 290, 727, 1189
Eam1104I CTCTTC 2 cut(s) 1115, 1210
EarI CTCTTC 2 cut(s) 1115, 1210
EciI GGCGGA 2 cut(s) 471, 965
Ecl136II GAGCTC 3 cut(s) 6, 196, 1049
Eco130I CCWWGG 3 cut(s) 34, 439, 1466
Eco24I GRGCYC 3 cut(s) 8, 198, 1051
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 3 cut(s) 364, 1076, 1463
Eco53kI GAGCTC 3 cut(s) 6, 196, 1049
Eco57I CTGAAG 1 cut(s) 483
Eco81I CCTNAGG 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 404
Eco91I GGTNACC 1 cut(s) 92
EcoICRI GAGCTC 3 cut(s) 6, 196, 1049
EcoNI CCTNNNNNAGG 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 1076
EcoO65I GGTNACC 1 cut(s) 92
EcoT14I CCWWGG 3 cut(s) 34, 439, 1466
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 3 cut(s) 8, 198, 1051
ErhI CCWWGG 3 cut(s) 34, 439, 1466
Esp3I CGTCTC 2 cut(s) 1281, 1458
FaeI CATG 7 cut(s) 745, 832, 929, 1033, 1208, 1268, 1470
FalI AAGNNNNNCTT 2 cut(s) 113, 145
FaqI GGGAC 1 cut(s) 1062
FatI CATG 7 cut(s) 741, 828, 925, 1029, 1204, 1264, 1466
FblI GTMKAC 2 cut(s) 43, 270
Fnu4HI GCNGC 5 cut(s) 165, 501, 504, 1103, 1250
FokI GGATG 6 cut(s) 194, 965, 1001, 1294, 1297, 1372
FriOI GRGCYC 3 cut(s) 8, 198, 1051
Fsp4HI GCNGC 5 cut(s) 165, 501, 504, 1103, 1250
FspBI CTAG 1 cut(s) 1458
GlaI GCGC 3 cut(s) 15, 416, 507
GluI GCNGC 5 cut(s) 165, 501, 504, 1103, 1250
GsuI CTGGAG 3 cut(s) 30, 194, 732
HaeIII GGCC 3 cut(s) 39, 438, 490
HapII CCGG 1 cut(s) 179
HgaI GACGC 2 cut(s) 890, 1263
HhaI GCGC 3 cut(s) 16, 417, 508
Hin1I GRCGYC 1 cut(s) 1274
Hin1II CATG 7 cut(s) 745, 832, 929, 1033, 1208, 1268, 1470
Hin6I GCGC 3 cut(s) 14, 415, 506
HinP1I GCGC 3 cut(s) 14, 415, 506
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 2 cut(s) 917, 1178
HinfI GANTC 7 cut(s) 45, 133, 395, 421, 589, 937, 1243
HpaII CCGG 1 cut(s) 179
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 5 cut(s) 44, 70, 271, 298, 307
Hpy188I TCNGA 5 cut(s) 27, 171, 260, 801, 1281
Hpy188III TCNNGA 8 cut(s) 9, 58, 191, 372, 425, 461, 880, 980
Hpy8I GTNNAC 5 cut(s) 44, 70, 271, 298, 307
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 3 cut(s) 458, 636, 952
HpyCH4III ACNGT 5 cut(s) 65, 268, 1376, 1418, 1452
HpyF10VI GCNNNNNNNGC 2 cut(s) 1046, 1255
HpyF3I CTNAG 8 cut(s) 168, 576, 735, 786, 798, 909, 1094, 1344
Hsp92I GRCGYC 1 cut(s) 1274
Hsp92II CATG 7 cut(s) 745, 832, 929, 1033, 1208, 1268, 1470
HspAI GCGC 3 cut(s) 14, 415, 506
KflI GGGWCCC 1 cut(s) 1076
Kzo9I GATC 6 cut(s) 27, 147, 260, 290, 727, 1189
LguI GCTCTTC 1 cut(s) 1210
LmnI GCTCC 3 cut(s) 11, 207, 1046
Lsp1109I GCAGC 2 cut(s) 1114, 1261
LweI GCATC 1 cut(s) 1350
MaeI CTAG 1 cut(s) 1458
MaeIII GTNAC 4 cut(s) 92, 317, 668, 1412
MalI GATC 6 cut(s) 29, 149, 262, 292, 729, 1191
MbiI CCGCTC 1 cut(s) 167
MboI GATC 6 cut(s) 27, 147, 260, 290, 727, 1189
MboII GAAGA 6 cut(s) 107, 449, 1102, 1227, 1252, 1484
MfeI CAATTG 1 cut(s) 1035
MflI RGATCY 3 cut(s) 27, 260, 290
MhlI GDGCHC 3 cut(s) 8, 198, 1051
MluCI AATT 6 cut(s) 601, 685, 802, 841, 1035, 1320
MlyI GAGTC 3 cut(s) 39, 404, 931
MmeI TCCRAC 1 cut(s) 229
Mph1103I ATGCAT 1 cut(s) 474
MroXI GAANNNNTTC 2 cut(s) 472, 1139
MseI TTAA 6 cut(s) 570, 615, 648, 684, 1053, 1176
MslI CAYNNNNRTG 1 cut(s) 1209
MspCI CTTAAG 1 cut(s) 1052
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 179
MunI CAATTG 1 cut(s) 1035
Mva1269I GAATGC 3 cut(s) 472, 474, 1479
MvnI CGCG 1 cut(s) 508
MwoI GCNNNNNNNGC 2 cut(s) 1046, 1255
NciI CCSGG 1 cut(s) 179
NcoI CCATGG 1 cut(s) 1466
NdeII GATC 6 cut(s) 27, 147, 260, 290, 727, 1189
NlaIII CATG 7 cut(s) 745, 832, 929, 1033, 1208, 1268, 1470
NlaIV GGNNCC 5 cut(s) 203, 262, 1077, 1078, 1373
NmeAIII GCCGAG 1 cut(s) 510
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 2 cut(s) 745, 1208
PaeR7I CTCGAG 1 cut(s) 404
PaqCI CACCTGC 1 cut(s) 514
PciI ACATGT 1 cut(s) 741
PciSI GCTCTTC 1 cut(s) 1210
PctI GAATGC 3 cut(s) 472, 474, 1479
PdmI GAANNNNTTC 2 cut(s) 472, 1139
PfeI GAWTC 4 cut(s) 133, 421, 589, 1243
PkrI GCNGC 5 cut(s) 166, 502, 505, 1104, 1251
PleI GAGTC 3 cut(s) 39, 403, 931
PpsI GAGTC 3 cut(s) 39, 403, 931
PpuMI RGGWCCY 1 cut(s) 1076
PscI ACATGT 1 cut(s) 741
Psp124BI GAGCTC 3 cut(s) 8, 198, 1051
Psp5II RGGWCCY 1 cut(s) 1076
PspEI GGTNACC 1 cut(s) 92
PspN4I GGNNCC 5 cut(s) 203, 262, 1077, 1078, 1373
PspPI GGNCC 3 cut(s) 364, 1076, 1463
PspPPI RGGWCCY 1 cut(s) 1076
PspXI VCTCGAGB 1 cut(s) 404
PstI CTGCAG 1 cut(s) 1251
PstNI CAGNNNCTG 1 cut(s) 1255
PsuI RGATCY 3 cut(s) 27, 260, 290
RsaI GTAC 1 cut(s) 809
RsaNI GTAC 1 cut(s) 808
RseI CAYNNNNRTG 1 cut(s) 1209
SacI GAGCTC 3 cut(s) 8, 198, 1051
SapI GCTCTTC 1 cut(s) 1210
SaqAI TTAA 6 cut(s) 570, 615, 648, 684, 1053, 1176
SatI GCNGC 5 cut(s) 165, 501, 504, 1103, 1250
Sau3AI GATC 6 cut(s) 27, 147, 260, 290, 727, 1189
Sau96I GGNCC 3 cut(s) 364, 1076, 1463
SchI GAGTC 3 cut(s) 39, 404, 931
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 3 cut(s) 8, 198, 1051
SfaNI GCATC 1 cut(s) 1350
SfcI CTRYAG 1 cut(s) 1247
Sfr274I CTCGAG 1 cut(s) 404
SinI GGWCC 3 cut(s) 364, 1076, 1463
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 1209
SmlI CTYRAG 4 cut(s) 404, 425, 1052, 1235
SmoI CTYRAG 4 cut(s) 404, 425, 1052, 1235
Sse9I AATT 6 cut(s) 601, 685, 802, 841, 1035, 1320
SsiI CCGC 7 cut(s) 165, 482, 500, 503, 869, 950, 1401
SspI AATATT 1 cut(s) 568
SspMI CTAG 1 cut(s) 1458
SstI GAGCTC 3 cut(s) 8, 198, 1051
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 3 cut(s) 34, 439, 1466
TaaI ACNGT 5 cut(s) 65, 268, 1376, 1418, 1452
TaqI TCGA 3 cut(s) 405, 881, 1188
TasI AATT 6 cut(s) 601, 685, 802, 841, 1035, 1320
TauI GCSGC 3 cut(s) 167, 503, 506
TfiI GAWTC 4 cut(s) 133, 421, 589, 1243
Tru1I TTAA 6 cut(s) 570, 615, 648, 684, 1053, 1176
Tru9I TTAA 6 cut(s) 570, 615, 648, 684, 1053, 1176
TscAI CASTG 7 cut(s) 70, 232, 271, 790, 1348, 1423, 1500
TseI GCWGC 2 cut(s) 1102, 1249
TspDTI ATGAA 5 cut(s) 188, 618, 743, 914, 1253
TspGWI ACGGA 1 cut(s) 283
TspRI CASTG 7 cut(s) 70, 232, 271, 790, 1348, 1423, 1500
Vha464I CTTAAG 1 cut(s) 1052
VpaK11BI GGWCC 3 cut(s) 364, 1076, 1463
XagI CCTNNNNNAGG 1 cut(s) 443
XceI RCATGY 2 cut(s) 745, 1208
XhoI CTCGAG 1 cut(s) 404
XmiI GTMKAC 2 cut(s) 43, 270
XmnI GAANNNNTTC 2 cut(s) 472, 1139
XspI CTAG 1 cut(s) 1458
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.