Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0003859.1

Orthogroup ID

OG0002307

Physical Location & Seq

Genomic Coordinates Forward (+)

153892 .. 155235

Gene Structure 1344 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0003859.1_g000044.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

atgaatctagttcacattcatcagctccgactccaactccattacaatctggttagtgtcatcactagctctgctattcttgttttggcttccaccatttacattatgactcgacccagatccgtttacctcgtagactactcgtgctataagccccctaacaattggaaagtgaaataccagaagtttattaatcactccagattgatcggggcctttgatgagtcctcgctcgagtttcagcacatgattcttaagcggtctggccttggacaggagacctatctccctgaagcaatgctttccattcctcggaggctgtcaatggcggcagcgcgtgaggaggcggagcaggtgatttttggagcgttggataatctatttgcaaatactaatgtcaatcctaaggacattgggattcttgttatgaattctagtctgtttaatccaactccttcattgtcttctatgattgttaataagtacaaattgaggggaagtattaagagttataatttgggaggtatgggatgtagtgctggagttatagctgtagatcttgctaagcacctgttgcaagttcaaaagaacacttatgcagttgttgtcagcactgagaatgttactcaaaattggtaccttgggaacaagaaatccatgttggtacaaaattgtttgtttcgtgtgggtgggtctgcggttttgctctcgaacaagttatctgataggtgtagggctaagtacaagcttgtgcatatcatgaggactcatataggctctaatgataaggcatataggtgtgcttatcaagaggaggatgatgccagaaaacctggggtttcgttatcgaaggagctcatggcgattgctggtgtagcactcaagactaatatcacagctttgctcggtcctcttgtcctacctataagcgaccagcttctcttcattgctactttccttgctgacaagttgttcaatgcgaaagtcaagccttacatcccgaatttcaaactcgttttcgatcatttctgcatccatgctggtggaaggtctgtgattaatgagcttgagaagaatttgcagcttctaccagttcatgttgaggcgtcacggatgacactccacaggtttggtaacacctcctccagctcgatttggtatgagttggcttacaccgaggcaaaagggaggatacaaaaggggaaccgtgtgtggcagattgcgctcggaagcggattcaagtgtaatagcgtagtctgggaggctctccagaaagtgaaaccttctcccaatagcccttgggaagattgcattgattggtatccagtgcaaacagttctctag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004312 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009611 GO:0009628 GO:0009698 GO:0009699 GO:0009922 GO:0009987 GO:0010345 GO:0012505 GO:0016740 GO:0016746 GO:0016747 GO:0019438 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0050896 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00062 ko01110 map00062 map01110

Pfam Domains

Protein Families

ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

447

Amino Acids

50.14

Weight (kDa)

9.47

Isoelectric Point (pI)

34.23

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16280 AT4G34510
fragaria_vesca	FvH4_2g08940 FvH4_2g08940
malus_domestica	MD05G1065300.v1.1 MD10G1075000.v1.1
prunus_persica	Prupe.8G096100_v2.0.a1
pyrus_communis	pycom05g05670 pycom10g05860
rosa_chinensis	RchiOBHm_Chr2g0164781 RchiOBHm_Chr6g0264551 RchiOBHm_Chr6g0266411 RchiOBHm_Chr6g0266471 RchiOBHm_Chr6g0266501
rosa_laevigata	RLG00000014100 RLG00000014245
rosa_multiflora	Rmu_co8165060.1_g000001 Rmu_sc0000578.1_g000014 Rmu_sc0000946.1_g000023 Rmu_sc0002235.1_g000037 Rmu_sc0003605.1_g000018 Rmu_sc0003859.1_g000044 Rmu_sc0008317.1_g000012
rosa_roxburghii	Rroxscaffold_175G00431910 Rroxscaffold_7G00201710
rosa_rugosa	Rorug06G0015600
rosa_samantha	Rh2BG589600 Rh2CG560200 Rh6AG133300 Rh6AG145800 Rh6AG146600 Rh6BG130800 Rh6BG146200 Rh6BG146600 Rh6CG129000 Rh6CG142700 Rh6DG116200 Rh6DG130800 Rh6DG131300
rosa_wichuraiana	Rw0G009730 Rw2G048020 Rw6G012740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	513
AarI	CACCTGC	1 cut(s)	343
Acc36I	ACCTGC	1 cut(s)	343
Acc65I	GGTACC	1 cut(s)	636
AccB1I	GGYRCC	1 cut(s)	636
AccI	GTMKAC	1 cut(s)	135
AccII	CGCG	1 cut(s)	337
AciI	CCGC	5 cut(s)	259, 329, 347, 698, 1233
AclWI	GGATC	1 cut(s)	114
AcsI	RAATTY	3 cut(s)	430, 1003, 1075
AcuI	CTGAAG	1 cut(s)	312
AcyI	GRCGYC	1 cut(s)	1106
AfaI	GTAC	4 cut(s)	485, 638, 666, 743
AfiI	CCNNNNNNNGG	2 cut(s)	274, 312
AflII	CTTAAG	1 cut(s)	254
AgsI	TTSAA	4 cut(s)	584, 976, 1009, 1240
AjnI	CCWGG	1 cut(s)	832
AjuI	GAANNNNNNNTTGG	2 cut(s)	644, 676
AloI	GAACNNNNNNTCC	2 cut(s)	638, 670
Alw21I	GWGCWC	1 cut(s)	858
Alw26I	GTCTC	1 cut(s)	272
AlwI	GGATC	1 cut(s)	114
Ama87I	CYCGRG	1 cut(s)	233
AoxI	GGCC	2 cut(s)	213, 265
ApeKI	GCWGC	2 cut(s)	332, 1081
ApoI	RAATTY	3 cut(s)	430, 1003, 1075
AseI	ATTAAT	2 cut(s)	192, 1059
Asp718I	GGTACC	1 cut(s)	636
AspLEI	GCGC	2 cut(s)	337, 1225
AspS9I	GGNCC	2 cut(s)	213, 908
AsuHPI	GGTGA	1 cut(s)	367
AvaI	CYCGRG	1 cut(s)	233
AvaII	GGWCC	1 cut(s)	908
AxyI	CCTNAGG	1 cut(s)	405
BanI	GGYRCC	1 cut(s)	636
BanII	GRGCYC	1 cut(s)	858
BauI	CACGAG	1 cut(s)	142
BbsI	GAAGAC	1 cut(s)	456
Bbv12I	GWGCWC	1 cut(s)	858
BbvI	GCAGC	2 cut(s)	344, 1093
BciT130I	CCWGG	1 cut(s)	834
BciVI	GTATCC	2 cut(s)	1185, 1332
BcoDI	GTCTC	1 cut(s)	272
BfaI	CTAG	4 cut(s)	8, 66, 435, 1342
BfmI	CTRYAG	1 cut(s)	552
BfrI	CTTAAG	1 cut(s)	254
BfuAI	ACCTGC	1 cut(s)	343
BfuI	GTATCC	2 cut(s)	1185, 1332
BglII	AGATCT	1 cut(s)	556
BisI	GCNGC	3 cut(s)	330, 333, 1082
BlpI	GCTNAGC	1 cut(s)	564
BlsI	GCNGC	3 cut(s)	331, 334, 1083
Bme1390I	CCNGG	1 cut(s)	834
Bme18I	GGWCC	1 cut(s)	908
BmeT110I	CYCGRG	1 cut(s)	233
BmgT120I	GGNCC	2 cut(s)	213, 908
BmiI	GGNNCC	3 cut(s)	214, 638, 1205
BmrFI	CCNGG	1 cut(s)	834
BmsI	GCATC	2 cut(s)	811, 1041
BpiI	GAAGAC	1 cut(s)	456
BpmI	CTGGAG	4 cut(s)	184, 561, 1129, 1253
Bpu1102I	GCTNAGC	1 cut(s)	564
BpuEI	CTTGAG	2 cut(s)	866, 1088
BsaBI	GATNNNNATC	1 cut(s)	1320
BsaHI	GRCGYC	1 cut(s)	1106
BsaI	GGTCTC	1 cut(s)	272
BsaJI	CCNNGG	6 cut(s)	268, 311, 640, 833, 1176, 1298
BsaXI	ACNNNNNCTCC	4 cut(s)	21, 51, 1127, 1157
Bsc4I	CCNNNNNNNGG	2 cut(s)	274, 312
Bse1I	ACTGG	2 cut(s)	1091, 1325
Bse21I	CCTNAGG	1 cut(s)	405
Bse3DI	GCAATG	2 cut(s)	303, 945
Bse8I	GATNNNNATC	1 cut(s)	1320
BseBI	CCWGG	1 cut(s)	834
BseDI	CCNNGG	6 cut(s)	268, 311, 640, 833, 1176, 1298
BseGI	GGATG	5 cut(s)	536, 823, 996, 1032, 1119
BseJI	GATNNNNATC	1 cut(s)	1320
BseLI	CCNNNNNNNGG	2 cut(s)	274, 312
BseMI	GCAATG	2 cut(s)	303, 945
BseMII	CTCAG	1 cut(s)	606
BseNI	ACTGG	2 cut(s)	1091, 1325
BseRI	GAGGAG	3 cut(s)	356, 827, 1132
BseXI	GCAGC	2 cut(s)	344, 1093
Bsh1236I	CGCG	1 cut(s)	337
BshFI	GGCC	2 cut(s)	215, 267
BshNI	GGYRCC	1 cut(s)	636
BsiHKAI	GWGCWC	1 cut(s)	858
BsiHKCI	CYCGRG	1 cut(s)	233
BslI	CCNNNNNNNGG	2 cut(s)	274, 312
BsmAI	GTCTC	1 cut(s)	272
BsnI	GGCC	2 cut(s)	215, 267
Bso31I	GGTCTC	1 cut(s)	272
BsoBI	CYCGRG	1 cut(s)	233
Bsp1286I	GDGCHC	1 cut(s)	858
Bsp143I	GATC	4 cut(s)	119, 207, 556, 1021
Bsp1720I	GCTNAGC	1 cut(s)	564
BspACI	CCGC	5 cut(s)	259, 329, 347, 698, 1233
BspANI	GGCC	2 cut(s)	215, 267
BspCNI	CTCAG	1 cut(s)	607
BspFNI	CGCG	1 cut(s)	337
BspHI	TCATGA	1 cut(s)	759
BspLI	GGNNCC	3 cut(s)	214, 638, 1205
BspMI	ACCTGC	1 cut(s)	343
BspPI	GGATC	1 cut(s)	114
BspT107I	GGYRCC	1 cut(s)	636
BspTI	CTTAAG	1 cut(s)	254
BspTNI	GGTCTC	1 cut(s)	272
BsrDI	GCAATG	2 cut(s)	303, 945
BsrI	ACTGG	2 cut(s)	1091, 1325
BssECI	CCNNGG	6 cut(s)	268, 311, 640, 833, 1176, 1298
BssMI	GATC	4 cut(s)	119, 207, 556, 1021
BssNI	GRCGYC	1 cut(s)	1106
BssSI	CACGAG	1 cut(s)	142
BssT1I	CCWWGG	3 cut(s)	268, 640, 1298
Bst2BI	CACGAG	1 cut(s)	142
Bst2UI	CCWGG	1 cut(s)	834
Bst4CI	ACNGT	2 cut(s)	1208, 1336
Bst6I	CTCTTC	1 cut(s)	947
BstACI	GRCGYC	1 cut(s)	1106
BstAFI	CTTAAG	1 cut(s)	254
BstAPI	GCANNNNNTGC	1 cut(s)	574
BstDEI	CTNAG	4 cut(s)	405, 564, 615, 738
BstENI	CCTNNNNNAGG	1 cut(s)	272
BstF5I	GGATG	5 cut(s)	536, 823, 996, 1032, 1119
BstFNI	CGCG	1 cut(s)	337
BstHHI	GCGC	2 cut(s)	337, 1225
BstKTI	GATC	4 cut(s)	122, 210, 559, 1024
BstMAI	GTCTC	1 cut(s)	272
BstMBI	GATC	4 cut(s)	119, 207, 556, 1021
BstMWI	GCNNNNNNNGC	3 cut(s)	574, 875, 1222
BstNI	CCWGG	1 cut(s)	834
BstSCI	CCNGG	1 cut(s)	832
BstSFI	CTRYAG	1 cut(s)	552
BstUI	CGCG	1 cut(s)	337
BstV1I	GCAGC	2 cut(s)	344, 1093
BstV2I	GAAGAC	1 cut(s)	456
BstX2I	RGATCY	2 cut(s)	119, 556
BstXI	CCANNNNNNTGG	2 cut(s)	1043, 1130
BstYI	RGATCY	2 cut(s)	119, 556
Bsu36I	CCTNAGG	1 cut(s)	405
BsuI	GTATCC	2 cut(s)	1185, 1332
BsuRI	GGCC	2 cut(s)	215, 267
BtsCI	GGATG	5 cut(s)	536, 823, 996, 1032, 1119
BtsIMutI	CAGTG	2 cut(s)	612, 1332
BveI	ACCTGC	1 cut(s)	343
CciI	TCATGA	1 cut(s)	759
CfoI	GCGC	2 cut(s)	337, 1225
Cfr13I	GGNCC	2 cut(s)	213, 908
CseI	GACGC	1 cut(s)	1095
Csp6I	GTAC	4 cut(s)	484, 637, 665, 742
CviAII	CATG	6 cut(s)	247, 658, 760, 859, 1037, 1097
CviQI	GTAC	4 cut(s)	484, 637, 665, 742
DdeI	CTNAG	4 cut(s)	405, 564, 615, 738
DpnI	GATC	4 cut(s)	121, 209, 558, 1023
DpnII	GATC	4 cut(s)	119, 207, 556, 1021
Eam1104I	CTCTTC	1 cut(s)	947
EarI	CTCTTC	1 cut(s)	947
EciI	GGCGGA	1 cut(s)	362
Ecl136II	GAGCTC	1 cut(s)	856
Eco130I	CCWWGG	3 cut(s)	268, 640, 1298
Eco24I	GRGCYC	1 cut(s)	858
Eco31I	GGTCTC	1 cut(s)	272
Eco47I	GGWCC	1 cut(s)	908
Eco53kI	GAGCTC	1 cut(s)	856
Eco57I	CTGAAG	1 cut(s)	312
Eco81I	CCTNAGG	1 cut(s)	405
Eco88I	CYCGRG	1 cut(s)	233
EcoICRI	GAGCTC	1 cut(s)	856
EcoNI	CCTNNNNNAGG	1 cut(s)	272
EcoO109I	RGGNCCY	1 cut(s)	213
EcoRI	GAATTC	1 cut(s)	430
EcoRII	CCWGG	1 cut(s)	832
EcoT14I	CCWWGG	3 cut(s)	268, 640, 1298
EcoT38I	GRGCYC	1 cut(s)	858
ErhI	CCWWGG	3 cut(s)	268, 640, 1298
FaeI	CATG	6 cut(s)	250, 661, 763, 862, 1040, 1100
FalI	AAGNNNNNCTT	2 cut(s)	285, 317
FatI	CATG	6 cut(s)	246, 657, 759, 858, 1036, 1096
FblI	GTMKAC	1 cut(s)	135
Fnu4HI	GCNGC	3 cut(s)	330, 333, 1082
FokI	GGATG	5 cut(s)	543, 830, 983, 1019, 1126
FriOI	GRGCYC	1 cut(s)	858
Fsp4HI	GCNGC	3 cut(s)	330, 333, 1082
FspBI	CTAG	4 cut(s)	8, 66, 435, 1342
GlaI	GCGC	2 cut(s)	336, 1224
GluI	GCNGC	3 cut(s)	330, 333, 1082
GsuI	CTGGAG	4 cut(s)	184, 561, 1129, 1253
HaeIII	GGCC	2 cut(s)	215, 267
HgaI	GACGC	1 cut(s)	1095
HhaI	GCGC	2 cut(s)	337, 1225
Hin1I	GRCGYC	1 cut(s)	1106
Hin1II	CATG	6 cut(s)	250, 661, 763, 862, 1040, 1100
Hin6I	GCGC	2 cut(s)	335, 1223
HinP1I	GCGC	2 cut(s)	335, 1223
HindIII	AAGCTT	1 cut(s)	746
HinfI	GANTC	8 cut(s)	4, 30, 109, 224, 250, 418, 766, 1236
HphI	GGTGA	1 cut(s)	367
Hpy166II	GTNNAC	3 cut(s)	13, 127, 136
Hpy188I	TCNGA	4 cut(s)	29, 315, 724, 1229
Hpy188III	TCNNGA	7 cut(s)	201, 709, 760, 809, 883, 1000, 1270
Hpy8I	GTNNAC	3 cut(s)	13, 127, 136
HpyAV	CCTTC	4 cut(s)	465, 844, 1041, 1293
HpyCH4III	ACNGT	2 cut(s)	1208, 1336
HpyCH4V	TGCA	8 cut(s)	386, 577, 599, 754, 1032, 1081, 1311, 1330
HpyF10VI	GCNNNNNNNGC	3 cut(s)	574, 875, 1222
HpyF3I	CTNAG	4 cut(s)	405, 564, 615, 738
Hsp92I	GRCGYC	1 cut(s)	1106
Hsp92II	CATG	6 cut(s)	250, 661, 763, 862, 1040, 1100
HspAI	GCGC	2 cut(s)	335, 1223
KpnI	GGTACC	1 cut(s)	640
Kzo9I	GATC	4 cut(s)	119, 207, 556, 1021
LmnI	GCTCC	4 cut(s)	30, 349, 365, 853
Lsp1109I	GCAGC	2 cut(s)	344, 1093
LweI	GCATC	2 cut(s)	811, 1041
MaeI	CTAG	4 cut(s)	8, 66, 435, 1342
MaeIII	GTNAC	3 cut(s)	622, 1107, 1133
MalI	GATC	4 cut(s)	121, 209, 558, 1023
MboI	GATC	4 cut(s)	119, 207, 556, 1021
MboII	GAAGA	4 cut(s)	456, 934, 1084, 1316
MfeI	CAATTG	1 cut(s)	163
MflI	RGATCY	2 cut(s)	119, 556
MhlI	GDGCHC	1 cut(s)	858
MluCI	AATT	8 cut(s)	163, 430, 488, 514, 631, 670, 1003, 1075
MlyI	GAGTC	4 cut(s)	24, 103, 233, 760
MmeI	TCCRAC	4 cut(s)	52, 58, 351, 473
MseI	TTAA	6 cut(s)	192, 255, 444, 477, 504, 1059
MslI	CAYNNNNRTG	2 cut(s)	796, 1041
MspCI	CTTAAG	1 cut(s)	254
MspR9I	CCNGG	1 cut(s)	834
MunI	CAATTG	1 cut(s)	163
MvaI	CCWGG	1 cut(s)	834
MvnI	CGCG	1 cut(s)	337
MwoI	GCNNNNNNNGC	3 cut(s)	574, 875, 1222
NdeII	GATC	4 cut(s)	119, 207, 556, 1021
NlaIII	CATG	6 cut(s)	250, 661, 763, 862, 1040, 1100
NlaIV	GGNNCC	3 cut(s)	214, 638, 1205
NmuCI	GTSAC	1 cut(s)	1107
PaeR7I	CTCGAG	1 cut(s)	233
PagI	TCATGA	1 cut(s)	759
PaqCI	CACCTGC	1 cut(s)	343
PfeI	GAWTC	4 cut(s)	4, 250, 418, 1236
PkrI	GCNGC	3 cut(s)	331, 334, 1083
PleI	GAGTC	4 cut(s)	24, 103, 232, 760
PpsI	GAGTC	4 cut(s)	24, 103, 232, 760
PshBI	ATTAAT	2 cut(s)	192, 1059
PsiI	TTATAA	1 cut(s)	513
Psp124BI	GAGCTC	1 cut(s)	858
Psp6I	CCWGG	1 cut(s)	832
PspGI	CCWGG	1 cut(s)	832
PspN4I	GGNNCC	3 cut(s)	214, 638, 1205
PspPI	GGNCC	2 cut(s)	213, 908
PspXI	VCTCGAGB	1 cut(s)	233
PsuI	RGATCY	2 cut(s)	119, 556
RsaI	GTAC	4 cut(s)	485, 638, 666, 743
RsaNI	GTAC	4 cut(s)	484, 637, 665, 742
RseI	CAYNNNNRTG	2 cut(s)	796, 1041
SacI	GAGCTC	1 cut(s)	858
SaqAI	TTAA	6 cut(s)	192, 255, 444, 477, 504, 1059
SatI	GCNGC	3 cut(s)	330, 333, 1082
Sau3AI	GATC	4 cut(s)	119, 207, 556, 1021
Sau96I	GGNCC	2 cut(s)	213, 908
SchI	GAGTC	4 cut(s)	24, 103, 233, 760
ScrFI	CCNGG	1 cut(s)	834
SduI	GDGCHC	1 cut(s)	858
SfaNI	GCATC	2 cut(s)	811, 1041
SfcI	CTRYAG	1 cut(s)	552
Sfr274I	CTCGAG	1 cut(s)	233
SinI	GGWCC	1 cut(s)	908
SlaI	CTCGAG	1 cut(s)	233
SmiMI	CAYNNNNRTG	2 cut(s)	796, 1041
SmlI	CTYRAG	4 cut(s)	233, 254, 881, 1067
SmoI	CTYRAG	4 cut(s)	233, 254, 881, 1067
Sse9I	AATT	8 cut(s)	163, 430, 488, 514, 631, 670, 1003, 1075
SsiI	CCGC	5 cut(s)	259, 329, 347, 698, 1233
SspMI	CTAG	4 cut(s)	8, 66, 435, 1342
SstI	GAGCTC	1 cut(s)	858
StyD4I	CCNGG	1 cut(s)	832
StyI	CCWWGG	3 cut(s)	268, 640, 1298
TaaI	ACNGT	2 cut(s)	1208, 1336
TaqI	TCGA	6 cut(s)	112, 234, 710, 848, 1020, 1151
TaqII	GACCGA	1 cut(s)	896
TasI	AATT	8 cut(s)	163, 430, 488, 514, 631, 670, 1003, 1075
TatI	WGTACW	2 cut(s)	483, 741
TauI	GCSGC	1 cut(s)	332
TfiI	GAWTC	4 cut(s)	4, 250, 418, 1236
Tru1I	TTAA	6 cut(s)	192, 255, 444, 477, 504, 1059
Tru9I	TTAA	6 cut(s)	192, 255, 444, 477, 504, 1059
TscAI	CASTG	2 cut(s)	619, 1332
TseFI	GTSAC	1 cut(s)	1107
TseI	GCWGC	2 cut(s)	332, 1081
Tsp45I	GTSAC	1 cut(s)	1107
TspDTI	ATGAA	6 cut(s)	8, 17, 443, 447, 934, 1085
TspGWI	ACGGA	2 cut(s)	112, 1126
TspRI	CASTG	2 cut(s)	619, 1332
Vha464I	CTTAAG	1 cut(s)	254
VpaK11BI	GGWCC	1 cut(s)	908
VspI	ATTAAT	2 cut(s)	192, 1059
XagI	CCTNNNNNAGG	1 cut(s)	272
XapI	RAATTY	3 cut(s)	430, 1003, 1075
XhoI	CTCGAG	1 cut(s)	233
XmiI	GTMKAC	1 cut(s)	135
XspI	CTAG	4 cut(s)	8, 66, 435, 1342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.