Rh2BG589600

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002307
Physical Location & Seq
Forward (+)
80621243 .. 80623092
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG589600.1

Sequence Viewer

Length: 1311 bp
ATGAAGTATGTGAAGTTGGCTTCCCATTTCTTGACAACCAACCTCTTGAAACTCTTGTTAATTCTTGTAATAGCAGTCATTGTAATTGAAGCCTCTCAGATGAATCTAGATCACATTCATCAGCTCCGACTCCAACTCTGTTATAATCTGAAGTTTATTAATCAGTCCAGATTGACCGGGGCCTTTGATGAGTCCTCCCTCGAGTTTCAGCACAAGATTCTTGAGCGGTCTGGCCTTGGAGAGCAGACATATCTCCCTGAAGCAATGCATTTCATTCCGCCGAGGCTGTCAATGGCGGCAGCGCGTGAGGAGGCGGAGCAGGTGATTTTTGGAGCGTTGGATAATCTATTTGCAAATACTAATGTCAATCCTAAAGACATTGGGATTGTTGTTATGAATTCTAGTCTGTTTAATCCAATTCCTTCATTATCTTCTATGATTGTTAATAAGTACAAATTGAGGGGAAGTATTAAGAGTTATAATTTGGGAGGTATGGGATGTAGTGCTGGAGTTATAGCTGTAGATCTTGCTAAGGACCTGTTGCAAGTTCAAAAGAACACTTATGCAGTTGTTGTTAGCACTGAGAATATTACTAAAAATGGGTACCTTGGGAACAAGAAATCCATGTTAGTACAAAATTGTTTGTTTCGTGTGGGTGGGTCTGCGGTTTTGCTCTCGAACAAATCATCTGATATGTGTAGGGCCAAGTACAAGCTTGTGCATATCGTGAGGAATCATATGGGCTCGAATGATAGGGCATATAGGTGTGCTTATCAAGAGGAGGACGATGCTGGCAAAGCTGGGGTTTCGTTGTCGAAGGAGCTCATGGCGATTGCTGGTGTAGCGCTCAAGACTAATATCACAGCTTTGCTCGGTCCTCTTGTCCTACCTATAAGCGACCAGCTTCTCTTCATTGCTACTGTCCTTGCAAACAAGTTGTTCAATGCGAAAGTCAACCCTTACATCCCGAATTTCAAACTCGTTTTCGATCATTTTTGCATCCATGCTGGTGGAAGGTCTGTGATTGATGAGCTTGAGAAGAATTTGAAGCTTCTACCAGTTCATGTTGAGGCGTCACGGATGACACTCCACAGGTTTGGTAACACCTCCTCTAGCTCGATTTGGTATGAGTTGGCTTACACCGAGGCAAAGGGGAGGATACAAAAGGGGAACCGTGTGTGGCAGATTGCGCTCGGAAGTGGATTCAAGTGTAACAGTGTAGTCTGGGAGGCTCTCCGGAAAGCGAAACCTTCTCCCAGTAGCCCTTGGGAAGATTGCATTCATAGGTATCCGGTGCAGACTGTTCTCTAG

Protein Analysis

436

Amino Acids

48.37

Weight (kDa)

9.32

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 51 - 312 7.4e-120 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 330 - 410 7.5e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 332 - 390 2.6e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 144, 480
AarI CACCTGC 1 cut(s) 310
Acc36I ACCTGC 1 cut(s) 310
Acc65I GGTACC 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 603
AccBSI CCGCTC 1 cut(s) 226
AccII CGCG 1 cut(s) 304
AccIII TCCGGA 1 cut(s) 1236
AciI CCGC 5 cut(s) 226, 278, 296, 314, 665
AcsI RAATTY 3 cut(s) 397, 970, 1042
AcuI CTGAAG 2 cut(s) 170, 279
AcyI GRCGYC 1 cut(s) 1073
AfaI GTAC 4 cut(s) 452, 605, 633, 710
AfeI AGCGCT 1 cut(s) 846
AgsI TTSAA 7 cut(s) 49, 89, 551, 943, 976, 1048, 1207
AloI GAACNNNNNNTCC 2 cut(s) 605, 637
Alw21I GWGCWC 1 cut(s) 825
Ama87I CYCGRG 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 1236
Aor51HI AGCGCT 1 cut(s) 846
AoxI GGCC 3 cut(s) 180, 232, 702
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 3 cut(s) 397, 970, 1042
AseI ATTAAT 1 cut(s) 159
Asp718I GGTACC 1 cut(s) 603
AspLEI GCGC 3 cut(s) 304, 847, 1192
AspS9I GGNCC 4 cut(s) 180, 535, 702, 875
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 334
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 2 cut(s) 535, 875
BanI GGYRCC 1 cut(s) 603
BanII GRGCYC 2 cut(s) 746, 825
Bbv12I GWGCWC 1 cut(s) 825
BbvI GCAGC 1 cut(s) 311
BciVI GTATCC 2 cut(s) 1152, 1299
BcnI CCSGG 1 cut(s) 178
BfaI CTAG 4 cut(s) 107, 402, 1113, 1309
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 1 cut(s) 848
BfuAI ACCTGC 1 cut(s) 310
BfuI GTATCC 2 cut(s) 1152, 1299
BglII AGATCT 1 cut(s) 523
BisI GCNGC 2 cut(s) 297, 300
BlsI GCNGC 2 cut(s) 298, 301
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 2 cut(s) 535, 875
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 4 cut(s) 180, 535, 702, 875
BmiI GGNNCC 3 cut(s) 181, 605, 1172
BmrFI CCNGG 1 cut(s) 178
BmrI ACTGGG 1 cut(s) 1251
BmsI GCATC 2 cut(s) 778, 1008
BmuI ACTGGG 1 cut(s) 1251
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 1 cut(s) 531
BpuEI CTTGAG 3 cut(s) 242, 833, 1055
BpuMI CCSGG 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 1073
BsaJI CCNNGG 6 cut(s) 177, 235, 281, 607, 1143, 1265
BsaWI WCCGGW 2 cut(s) 1236, 1291
Bse1I ACTGG 2 cut(s) 1058, 1257
Bse3DI GCAATG 2 cut(s) 270, 912
BseAI TCCGGA 1 cut(s) 1236
BseDI CCNNGG 6 cut(s) 177, 235, 281, 607, 1143, 1265
BseGI GGATG 4 cut(s) 503, 963, 999, 1086
BseMI GCAATG 2 cut(s) 270, 912
BseMII CTCAG 2 cut(s) 110, 573
BseNI ACTGG 2 cut(s) 1058, 1257
BseRI GAGGAG 3 cut(s) 323, 794, 1099
BseXI GCAGC 1 cut(s) 311
BseYI CCCAGC 1 cut(s) 800
Bsh1236I CGCG 1 cut(s) 304
BshFI GGCC 3 cut(s) 182, 234, 704
BshNI GGYRCC 1 cut(s) 603
BsiHKAI GWGCWC 1 cut(s) 825
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 3 cut(s) 177, 1237, 1292
BsmI GAATGC 1 cut(s) 1278
BsnI GGCC 3 cut(s) 182, 234, 704
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 746, 825
Bsp13I TCCGGA 1 cut(s) 1236
Bsp143I GATC 3 cut(s) 109, 523, 988
BspACI CCGC 5 cut(s) 226, 278, 296, 314, 665
BspANI GGCC 3 cut(s) 182, 234, 704
BspCNI CTCAG 2 cut(s) 109, 574
BspEI TCCGGA 1 cut(s) 1236
BspFNI CGCG 1 cut(s) 304
BspLI GGNNCC 3 cut(s) 181, 605, 1172
BspMI ACCTGC 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 603
BsrBI CCGCTC 1 cut(s) 226
BsrDI GCAATG 2 cut(s) 270, 912
BsrI ACTGG 2 cut(s) 1058, 1257
BssECI CCNNGG 6 cut(s) 177, 235, 281, 607, 1143, 1265
BssMI GATC 3 cut(s) 109, 523, 988
BssNI GRCGYC 1 cut(s) 1073
BssT1I CCWWGG 3 cut(s) 235, 607, 1265
Bst4CI ACNGT 4 cut(s) 922, 1175, 1217, 1303
Bst6I CTCTTC 1 cut(s) 914
BstACI GRCGYC 1 cut(s) 1073
BstC8I GCNNGC 1 cut(s) 793
BstDEI CTNAG 3 cut(s) 96, 531, 582
BstF5I GGATG 4 cut(s) 503, 963, 999, 1086
BstFNI CGCG 1 cut(s) 304
BstH2I RGCGCY 1 cut(s) 848
BstHHI GCGC 3 cut(s) 304, 847, 1192
BstKTI GATC 3 cut(s) 112, 526, 991
BstMBI GATC 3 cut(s) 109, 523, 988
BstMWI GCNNNNNNNGC 3 cut(s) 797, 842, 1189
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 1 cut(s) 304
BstV1I GCAGC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 523
BstXI CCANNNNNNTGG 2 cut(s) 1010, 1097
BstYI RGATCY 1 cut(s) 523
BsuI GTATCC 2 cut(s) 1152, 1299
BsuRI GGCC 3 cut(s) 182, 234, 704
BtsCI GGATG 4 cut(s) 503, 963, 999, 1086
BtsIMutI CAGTG 2 cut(s) 579, 1222
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 793
CfoI GCGC 3 cut(s) 304, 847, 1192
Cfr13I GGNCC 4 cut(s) 180, 535, 702, 875
CseI GACGC 1 cut(s) 1062
Csp6I GTAC 4 cut(s) 451, 604, 632, 709
CviAII CATG 4 cut(s) 625, 826, 1004, 1064
CviQI GTAC 4 cut(s) 451, 604, 632, 709
DdeI CTNAG 3 cut(s) 96, 531, 582
DpnI GATC 3 cut(s) 111, 525, 990
DpnII GATC 3 cut(s) 109, 523, 988
Eam1104I CTCTTC 1 cut(s) 914
EarI CTCTTC 1 cut(s) 914
EciI GGCGGA 2 cut(s) 267, 329
Ecl136II GAGCTC 1 cut(s) 823
Eco130I CCWWGG 3 cut(s) 235, 607, 1265
Eco24I GRGCYC 2 cut(s) 746, 825
Eco47I GGWCC 2 cut(s) 535, 875
Eco47III AGCGCT 1 cut(s) 846
Eco53kI GAGCTC 1 cut(s) 823
Eco57I CTGAAG 2 cut(s) 170, 279
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 823
EcoO109I RGGNCCY 2 cut(s) 180, 535
EcoRI GAATTC 1 cut(s) 397
EcoT14I CCWWGG 3 cut(s) 235, 607, 1265
EcoT22I ATGCAT 1 cut(s) 270
EcoT38I GRGCYC 2 cut(s) 746, 825
ErhI CCWWGG 3 cut(s) 235, 607, 1265
FaeI CATG 4 cut(s) 628, 829, 1007, 1067
FatI CATG 4 cut(s) 624, 825, 1003, 1063
FauNDI CATATG 1 cut(s) 738
Fnu4HI GCNGC 2 cut(s) 297, 300
FokI GGATG 4 cut(s) 510, 950, 986, 1093
FriOI GRGCYC 2 cut(s) 746, 825
Fsp4HI GCNGC 2 cut(s) 297, 300
FspBI CTAG 4 cut(s) 107, 402, 1113, 1309
GlaI GCGC 3 cut(s) 303, 846, 1191
GluI GCNGC 2 cut(s) 297, 300
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 1 cut(s) 848
HaeIII GGCC 3 cut(s) 182, 234, 704
HapII CCGG 3 cut(s) 177, 1237, 1292
HgaI GACGC 1 cut(s) 1062
HhaI GCGC 3 cut(s) 304, 847, 1192
Hin1I GRCGYC 1 cut(s) 1073
Hin1II CATG 4 cut(s) 628, 829, 1007, 1067
Hin6I GCGC 3 cut(s) 302, 845, 1190
HinP1I GCGC 3 cut(s) 302, 845, 1190
HincII GTYRAC 1 cut(s) 955
HindII GTYRAC 1 cut(s) 955
HindIII AAGCTT 2 cut(s) 713, 1049
HinfI GANTC 6 cut(s) 103, 129, 191, 217, 733, 1203
HpaII CCGG 3 cut(s) 177, 1237, 1292
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 955
Hpy188I TCNGA 5 cut(s) 99, 128, 150, 691, 1196
Hpy8I GTNNAC 1 cut(s) 955
HpyAV CCTTC 4 cut(s) 432, 811, 1008, 1260
HpyCH4III ACNGT 4 cut(s) 922, 1175, 1217, 1303
HpyCH4V TGCA 9 cut(s) 268, 353, 544, 566, 721, 929, 999, 1278, 1297
HpyF10VI GCNNNNNNNGC 3 cut(s) 797, 842, 1189
HpyF3I CTNAG 3 cut(s) 96, 531, 582
Hsp92I GRCGYC 1 cut(s) 1073
Hsp92II CATG 4 cut(s) 628, 829, 1007, 1067
HspAI GCGC 3 cut(s) 302, 845, 1190
Kpn2I TCCGGA 1 cut(s) 1236
KpnI GGTACC 1 cut(s) 607
Kzo9I GATC 3 cut(s) 109, 523, 988
LmnI GCTCC 4 cut(s) 129, 316, 332, 820
Lsp1109I GCAGC 1 cut(s) 311
LweI GCATC 2 cut(s) 778, 1008
MaeI CTAG 4 cut(s) 107, 402, 1113, 1309
MaeIII GTNAC 3 cut(s) 1074, 1100, 1211
MalI GATC 3 cut(s) 111, 525, 990
MbiI CCGCTC 1 cut(s) 226
MboI GATC 3 cut(s) 109, 523, 988
MboII GAAGA 4 cut(s) 423, 901, 1051, 1283
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 2 cut(s) 746, 825
MluCI AATT 9 cut(s) 60, 84, 397, 417, 455, 481, 637, 970, 1042
MlyI GAGTC 2 cut(s) 123, 200
MmeI TCCRAC 3 cut(s) 151, 157, 318
Mph1103I ATGCAT 1 cut(s) 270
MroI TCCGGA 1 cut(s) 1236
MseI TTAA 5 cut(s) 59, 159, 411, 444, 471
MslI CAYNNNNRTG 2 cut(s) 763, 1008
MspI CCGG 3 cut(s) 177, 1237, 1292
MspR9I CCNGG 1 cut(s) 178
Mva1269I GAATGC 1 cut(s) 1278
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 3 cut(s) 797, 842, 1189
NciI CCSGG 1 cut(s) 178
NdeI CATATG 1 cut(s) 738
NdeII GATC 3 cut(s) 109, 523, 988
NlaIII CATG 4 cut(s) 628, 829, 1007, 1067
NlaIV GGNNCC 3 cut(s) 181, 605, 1172
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 1 cut(s) 1074
NsiI ATGCAT 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 200
PaqCI CACCTGC 1 cut(s) 310
PctI GAATGC 1 cut(s) 1278
PfeI GAWTC 4 cut(s) 103, 217, 733, 1203
PkrI GCNGC 2 cut(s) 298, 301
PleI GAGTC 2 cut(s) 123, 199
PpsI GAGTC 2 cut(s) 123, 199
PpuMI RGGWCCY 1 cut(s) 535
PshBI ATTAAT 1 cut(s) 159
PsiI TTATAA 2 cut(s) 144, 480
Psp124BI GAGCTC 1 cut(s) 825
Psp5II RGGWCCY 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 800
PspN4I GGNNCC 3 cut(s) 181, 605, 1172
PspPI GGNCC 4 cut(s) 180, 535, 702, 875
PspPPI RGGWCCY 1 cut(s) 535
PspXI VCTCGAGB 1 cut(s) 200
PsuI RGATCY 1 cut(s) 523
RsaI GTAC 4 cut(s) 452, 605, 633, 710
RsaNI GTAC 4 cut(s) 451, 604, 632, 709
RseI CAYNNNNRTG 2 cut(s) 763, 1008
SacI GAGCTC 1 cut(s) 825
SaqAI TTAA 5 cut(s) 59, 159, 411, 444, 471
SatI GCNGC 2 cut(s) 297, 300
Sau3AI GATC 3 cut(s) 109, 523, 988
Sau96I GGNCC 4 cut(s) 180, 535, 702, 875
SchI GAGTC 2 cut(s) 123, 200
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 2 cut(s) 746, 825
SfaNI GCATC 2 cut(s) 778, 1008
SfcI CTRYAG 1 cut(s) 519
Sfr274I CTCGAG 1 cut(s) 200
SinI GGWCC 2 cut(s) 535, 875
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 2 cut(s) 763, 1008
SmlI CTYRAG 4 cut(s) 200, 221, 848, 1034
SmoI CTYRAG 4 cut(s) 200, 221, 848, 1034
Sse9I AATT 9 cut(s) 60, 84, 397, 417, 455, 481, 637, 970, 1042
SsiI CCGC 5 cut(s) 226, 278, 296, 314, 665
SspI AATATT 1 cut(s) 589
SspMI CTAG 4 cut(s) 107, 402, 1113, 1309
SstI GAGCTC 1 cut(s) 825
StyD4I CCNGG 1 cut(s) 176
StyI CCWWGG 3 cut(s) 235, 607, 1265
TaaI ACNGT 4 cut(s) 922, 1175, 1217, 1303
TaqI TCGA 6 cut(s) 201, 677, 746, 815, 987, 1118
TaqII GACCGA 1 cut(s) 863
TasI AATT 9 cut(s) 60, 84, 397, 417, 455, 481, 637, 970, 1042
TatI WGTACW 3 cut(s) 450, 631, 708
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 4 cut(s) 103, 217, 733, 1203
Tru1I TTAA 5 cut(s) 59, 159, 411, 444, 471
Tru9I TTAA 5 cut(s) 59, 159, 411, 444, 471
TscAI CASTG 2 cut(s) 586, 1222
TseFI GTSAC 1 cut(s) 1074
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 1 cut(s) 1074
TspDTI ATGAA 9 cut(s) 17, 107, 116, 262, 410, 414, 901, 1052, 1271
TspGWI ACGGA 1 cut(s) 1093
TspRI CASTG 2 cut(s) 586, 1222
VpaK11BI GGWCC 2 cut(s) 535, 875
VspI ATTAAT 1 cut(s) 159
XapI RAATTY 3 cut(s) 397, 970, 1042
XbaI TCTAGA 1 cut(s) 106
XhoI CTCGAG 1 cut(s) 200
XspI CTAG 4 cut(s) 107, 402, 1113, 1309
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.