RchiOBHm_Chr3g0477761

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0009701
Physical Location & Seq
Reverse (-)
23696015 .. 23705486
9472 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44304

Sequence Viewer

Length: 645 bp
ATGGAAATCATTTATTTTCTCACTTTCATATTTTTTTTATTCCCCGCAACTGTGCCAGCACAGATAAACAAACCTTACGATATAGTCATGCTGGTCATCCAAAATCCTTATAACCAAGGTAGCGCTAGTCCTTGGACCATACATGGCCTGTGCCAGTTAATAATTGTCCTAACTATTATTTTGTGCCAGTTAATAATTGTCCATCAGCTCCTCCATTTAACCCTCGGTAGCGACGTTCAAGCTGCACTAACTAAAGCTTGGCCTGGTATTTCGGGTAAAGCAGACCTTAAATTTTGGGAACATGAATATGAGAAACACGGTTCATGTACCATAGATTTATTACCAACTTCTAATGACTACCTACTGAAAGGAGTTTACCTGTGGGAGCAAATCAAGTTTGATGATTTGATGGGTGTTGGTCCGGGTGCCCAATTTATGCCAAATACTCAATATGATGCGAACACGGTGGTGGCGGCCATCACAAGCAAATATAAGGTCAAAACAATATTGATCTGTGCGGATGACGCCATAGTGGATGTTAGGTTTTGTTATGTAGGAAGTTGGGTACTGGTAGATTGCCTGGACACCCAACCAGATCCCCAAACAGTTTGTACGGGTTTTATTCAGTATATTAGAAAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

214

Amino Acids

24.14

Weight (kDa)

5.19

Isoelectric Point (pI)

18.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 40 - 184 1.2e-15 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 425
AciI CCGC 3 cut(s) 45, 473, 518
AclWI GGATC 1 cut(s) 590
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 4 cut(s) 328, 567, 613, 641
AfeI AGCGCT 1 cut(s) 124
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 2 cut(s) 262, 579
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 3 cut(s) 208, 242, 257
AluI AGCT 3 cut(s) 208, 242, 257
AlwI GGATC 1 cut(s) 590
Aor51HI AGCGCT 1 cut(s) 124
AoxI GGCC 3 cut(s) 145, 260, 474
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 1 cut(s) 290
ArsI GACNNNNNNTTYG 2 cut(s) 480, 512
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 135, 419
AsuC2I CCSGG 1 cut(s) 423
AvaII GGWCC 2 cut(s) 135, 419
BaeGI GKGCMC 1 cut(s) 430
BanI GGYRCC 1 cut(s) 425
BbvI GCAGC 1 cut(s) 229
BccI CCATC 3 cut(s) 210, 403, 485
BciT130I CCWGG 2 cut(s) 264, 581
BcnI CCSGG 1 cut(s) 423
BfaI CTAG 2 cut(s) 126, 643
BfoI RGCGCY 1 cut(s) 126
BisI GCNGC 2 cut(s) 243, 474
BlsI GCNGC 2 cut(s) 244, 475
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 3 cut(s) 264, 423, 581
Bme18I GGWCC 2 cut(s) 135, 419
BmgT120I GGNCC 2 cut(s) 135, 419
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 3 cut(s) 264, 423, 581
BmsI GCATC 1 cut(s) 445
BpuMI CCSGG 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 3 cut(s) 115, 131, 223
Bse1I ACTGG 3 cut(s) 154, 187, 573
BseBI CCWGG 2 cut(s) 264, 581
BseDI CCNNGG 3 cut(s) 115, 131, 223
BseGI GGATG 3 cut(s) 96, 526, 541
BseNI ACTGG 3 cut(s) 154, 187, 573
BseRI GAGGAG 1 cut(s) 200
BseSI GKGCMC 1 cut(s) 430
BseXI GCAGC 1 cut(s) 229
BsgI GTGCAG 1 cut(s) 228
BshFI GGCC 3 cut(s) 147, 262, 476
BshNI GGYRCC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 422
BsnI GGCC 3 cut(s) 147, 262, 476
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 2 cut(s) 510, 595
BspACI CCGC 3 cut(s) 45, 473, 518
BspANI GGCC 3 cut(s) 147, 262, 476
BspLI GGNNCC 1 cut(s) 427
BspPI GGATC 1 cut(s) 590
BspT107I GGYRCC 1 cut(s) 425
BsrI ACTGG 3 cut(s) 154, 187, 573
BssECI CCNNGG 3 cut(s) 115, 131, 223
BssMI GATC 2 cut(s) 510, 595
BssNI GRCGYC 1 cut(s) 525
BssT1I CCWWGG 2 cut(s) 115, 131
Bst2UI CCWGG 2 cut(s) 264, 581
Bst4CI ACNGT 4 cut(s) 52, 320, 466, 607
BstACI GRCGYC 1 cut(s) 525
BstC8I GCNNGC 1 cut(s) 57
BstF5I GGATG 3 cut(s) 96, 526, 541
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 2 cut(s) 513, 598
BstMBI GATC 2 cut(s) 510, 595
BstMWI GCNNNNNNNGC 1 cut(s) 524
BstNI CCWGG 2 cut(s) 264, 581
BstSCI CCNGG 3 cut(s) 262, 421, 579
BstSLI GKGCMC 1 cut(s) 430
BstV1I GCAGC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BsuRI GGCC 3 cut(s) 147, 262, 476
BtsCI GGATG 3 cut(s) 96, 526, 541
Cac8I GCNNGC 1 cut(s) 57
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 135, 419
CseI GACGC 1 cut(s) 533
Csp6I GTAC 4 cut(s) 327, 566, 612, 640
CviAII CATG 4 cut(s) 88, 143, 302, 324
CviJI RGCY 6 cut(s) 147, 208, 242, 257, 262, 476
CviKI_1 RGCY 6 cut(s) 147, 208, 242, 257, 262, 476
CviQI GTAC 4 cut(s) 327, 566, 612, 640
DpnI GATC 2 cut(s) 512, 597
DpnII GATC 2 cut(s) 510, 595
EaeI YGGCCR 1 cut(s) 474
Eco130I CCWWGG 2 cut(s) 115, 131
Eco47I GGWCC 2 cut(s) 135, 419
Eco47III AGCGCT 1 cut(s) 124
EcoRII CCWGG 2 cut(s) 262, 579
EcoT14I CCWWGG 2 cut(s) 115, 131
ErhI CCWWGG 2 cut(s) 115, 131
FaeI CATG 4 cut(s) 91, 146, 305, 327
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 4 cut(s) 87, 142, 301, 323
FauI CCCGC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 243, 474
FokI GGATG 3 cut(s) 83, 533, 548
Fsp4HI GCNGC 2 cut(s) 243, 474
FspBI CTAG 2 cut(s) 126, 643
GlaI GCGC 1 cut(s) 124
GluI GCNGC 2 cut(s) 243, 474
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 3 cut(s) 147, 262, 476
HapII CCGG 1 cut(s) 422
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 4 cut(s) 91, 146, 305, 327
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 255
HpaII CCGG 1 cut(s) 422
Hpy166II GTNNAC 1 cut(s) 376
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 1 cut(s) 236
HpyCH4III ACNGT 4 cut(s) 52, 320, 466, 607
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 524
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 4 cut(s) 91, 146, 305, 327
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 2 cut(s) 510, 595
LmnI GCTCC 2 cut(s) 213, 385
Lsp1109I GCAGC 1 cut(s) 229
LweI GCATC 1 cut(s) 445
MaeI CTAG 2 cut(s) 126, 643
MaeII ACGT 1 cut(s) 234
MalI GATC 2 cut(s) 512, 597
MboI GATC 2 cut(s) 510, 595
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 430
MluCI AATT 4 cut(s) 162, 195, 290, 431
MnlI CCTC 2 cut(s) 221, 233
MseI TTAA 4 cut(s) 158, 191, 218, 288
MslI CAYNNNNRTG 2 cut(s) 306, 467
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 3 cut(s) 264, 423, 581
MvaI CCWGG 2 cut(s) 264, 581
MwoI GCNNNNNNNGC 1 cut(s) 524
NciI CCSGG 1 cut(s) 423
NdeII GATC 2 cut(s) 510, 595
NlaIII CATG 4 cut(s) 91, 146, 305, 327
NlaIV GGNNCC 1 cut(s) 427
OliI CACNNNNGTG 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PkrI GCNGC 2 cut(s) 244, 475
PsiI TTATAA 1 cut(s) 111
Psp6I CCWGG 2 cut(s) 262, 579
PspGI CCWGG 2 cut(s) 262, 579
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 2 cut(s) 135, 419
PsuI RGATCY 1 cut(s) 595
RsaI GTAC 4 cut(s) 328, 567, 613, 641
RsaNI GTAC 4 cut(s) 327, 566, 612, 640
RseI CAYNNNNRTG 2 cut(s) 306, 467
SaqAI TTAA 4 cut(s) 158, 191, 218, 288
SatI GCNGC 2 cut(s) 243, 474
Sau3AI GATC 2 cut(s) 510, 595
Sau96I GGNCC 2 cut(s) 135, 419
ScaI AGTACT 1 cut(s) 641
ScrFI CCNGG 3 cut(s) 264, 423, 581
SduI GDGCHC 1 cut(s) 430
SfaNI GCATC 1 cut(s) 445
SinI GGWCC 2 cut(s) 135, 419
SmiMI CAYNNNNRTG 2 cut(s) 306, 467
Sse9I AATT 4 cut(s) 162, 195, 290, 431
SsiI CCGC 3 cut(s) 45, 473, 518
SspI AATATT 1 cut(s) 507
SspMI CTAG 2 cut(s) 126, 643
StyD4I CCNGG 3 cut(s) 262, 421, 579
StyI CCWWGG 2 cut(s) 115, 131
TaaI ACNGT 4 cut(s) 52, 320, 466, 607
TaiI ACGT 1 cut(s) 237
TasI AATT 4 cut(s) 162, 195, 290, 431
TatI WGTACW 1 cut(s) 639
TauI GCSGC 1 cut(s) 476
Tru1I TTAA 4 cut(s) 158, 191, 218, 288
Tru9I TTAA 4 cut(s) 158, 191, 218, 288
TseI GCWGC 1 cut(s) 242
TspDTI ATGAA 3 cut(s) 16, 312, 318
VpaK11BI GGWCC 2 cut(s) 135, 419
XapI RAATTY 1 cut(s) 290
XspI CTAG 2 cut(s) 126, 643
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.